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P43791

- DYR_HAEIN

UniProt

P43791 - DYR_HAEIN

Protein

Dihydrofolate reductase

Gene

folA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei6 – 61Substrate; via carbonyl oxygenBy similarity
    Binding sitei8 – 81NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei28 – 281SubstrateBy similarity
    Binding sitei53 – 531SubstrateBy similarity
    Binding sitei58 – 581SubstrateBy similarity
    Binding sitei114 – 1141SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 207NADPBy similarity
    Nucleotide bindingi46 – 472NADPBy similarity
    Nucleotide bindingi64 – 652NADPBy similarity
    Nucleotide bindingi96 – 1038NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. response to antibiotic Source: UniProtKB-KW
    5. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic resistance, One-carbon metabolism, Trimethoprim resistance

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:folA
    Synonyms:folH
    Ordered Locus Names:HI_0899
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 160160Dihydrofolate reductasePRO_0000186391Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0899.

    Structurei

    3D structure databases

    ProteinModelPortaliP43791.
    SMRiP43791. Positions 3-160.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 159158DHFRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    KOiK00287.
    OMAiLTHIDLT.
    OrthoDBiEOG6KT2V2.
    PhylomeDBiP43791.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000194. DHFR. 1 hit.
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43791-1 [UniParc]FASTAAdd to Basket

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    MTFSLIVATT LNNVIGKDNQ MPWHLPADLA WFRQNTTGKP VIMGRKTFES    50
    IGRPLPKRTN IVLSRQLFEH EGVIWKDSFE SAVNFVRDFD EIMLIGGGEL 100
    FKQYLPKADK LYLTQIQTEL DGDTFFPQLN WEEWEIEFDE YRKADEQNRY 150
    DCRFLILTRK 160
    Length:160
    Mass (Da):18,913
    Last modified:November 1, 1995 - v1
    Checksum:i384A1D64092B8C4F
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti13 – 131N → S in strain: Isolate R1042 and Isolate R1047.
    Natural varianti21 – 211M → I in strain: Isolate R1047.
    Natural varianti54 – 541P → A in strain: Isolate R1047.
    Natural varianti56 – 561P → A in strain: Isolate R906.
    Natural varianti67 – 671L → P in strain: Isolate R1042.
    Natural varianti69 – 691E → K in strain: Isolate R1042.
    Natural varianti74 – 741I → V in strain: Isolate R1042.
    Natural varianti77 – 771D → N in strain: Isolate R1042.
    Natural varianti79 – 791F → L in strain: Isolate R1042.
    Natural varianti95 – 951I → L in strain: Isolate R1047.
    Natural varianti135 – 1351E → K in strain: Isolate R1042.
    Natural varianti142 – 1421R → H in strain: Isolate R1042.
    Natural varianti154 – 1541F → S in strain: Isolate R1042.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22559.1.
    X84207 Genomic DNA. Translation: CAA58993.1.
    X84205 Genomic DNA. Translation: CAA58991.1.
    X84206 Genomic DNA. Translation: CAA58992.1.
    PIRiC64101.
    S52336.
    S52337.
    S52338.
    RefSeqiNP_439060.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22559; AAC22559; HI_0899.
    GeneIDi949905.
    KEGGihin:HI0899.
    PATRICi20190455. VBIHaeInf48452_0941.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22559.1 .
    X84207 Genomic DNA. Translation: CAA58993.1 .
    X84205 Genomic DNA. Translation: CAA58991.1 .
    X84206 Genomic DNA. Translation: CAA58992.1 .
    PIRi C64101.
    S52336.
    S52337.
    S52338.
    RefSeqi NP_439060.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P43791.
    SMRi P43791. Positions 3-160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22559 ; AAC22559 ; HI_0899 .
    GeneIDi 949905.
    KEGGi hin:HI0899.
    PATRICi 20190455. VBIHaeInf48452_0941.

    Phylogenomic databases

    eggNOGi COG0262.
    KOi K00287.
    OMAi LTHIDLT.
    OrthoDBi EOG6KT2V2.
    PhylomeDBi P43791.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000194. DHFR. 1 hit.
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. de Groot R.
      Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Isolate R1042, Isolate R1047 and Isolate R906.

    Entry informationi

    Entry nameiDYR_HAEIN
    AccessioniPrimary (citable) accession number: P43791
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Isolates R906, R1042, and R1047 are trimethoprim-resistant.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3