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Protein

Dihydrofolate reductase

Gene

folA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Miscellaneous

Isolates R906, R1042, and R1047 are trimethoprim-resistant.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei6Substrate; via carbonyl oxygenBy similarity1
Binding sitei8NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei28SubstrateBy similarity1
Binding sitei53SubstrateBy similarity1
Binding sitei58SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 20NADPBy similarity7
Nucleotide bindingi46 – 47NADPBy similarity2
Nucleotide bindingi64 – 65NADPBy similarity2
Nucleotide bindingi96 – 103NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic resistance, One-carbon metabolism, Trimethoprim resistance
LigandNADP

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-939-MONOMER
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:folH
Ordered Locus Names:HI_0899
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863911 – 160Dihydrofolate reductaseAdd BLAST160

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0899

Structurei

3D structure databases

ProteinModelPortaliP43791
SMRiP43791
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 159DHFRPROSITE-ProRule annotationAdd BLAST158

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108YYV Bacteria
COG0262 LUCA
KOiK00287
OMAiRDNQLPW
PhylomeDBiP43791

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P43791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFSLIVATT LNNVIGKDNQ MPWHLPADLA WFRQNTTGKP VIMGRKTFES
60 70 80 90 100
IGRPLPKRTN IVLSRQLFEH EGVIWKDSFE SAVNFVRDFD EIMLIGGGEL
110 120 130 140 150
FKQYLPKADK LYLTQIQTEL DGDTFFPQLN WEEWEIEFDE YRKADEQNRY
160
DCRFLILTRK
Length:160
Mass (Da):18,913
Last modified:November 1, 1995 - v1
Checksum:i384A1D64092B8C4F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti13N → S in strain: Isolate R1042 and Isolate R1047. 1
Natural varianti21M → I in strain: Isolate R1047. 1
Natural varianti54P → A in strain: Isolate R1047. 1
Natural varianti56P → A in strain: Isolate R906. 1
Natural varianti67L → P in strain: Isolate R1042. 1
Natural varianti69E → K in strain: Isolate R1042. 1
Natural varianti74I → V in strain: Isolate R1042. 1
Natural varianti77D → N in strain: Isolate R1042. 1
Natural varianti79F → L in strain: Isolate R1042. 1
Natural varianti95I → L in strain: Isolate R1047. 1
Natural varianti135E → K in strain: Isolate R1042. 1
Natural varianti142R → H in strain: Isolate R1042. 1
Natural varianti154F → S in strain: Isolate R1042. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22559.1
X84207 Genomic DNA Translation: CAA58993.1
X84205 Genomic DNA Translation: CAA58991.1
X84206 Genomic DNA Translation: CAA58992.1
PIRiC64101
S52336
S52337
S52338
RefSeqiNP_439060.1, NC_000907.1
WP_005648125.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22559; AAC22559; HI_0899
GeneIDi949905
KEGGihin:HI0899
PATRICifig|71421.8.peg.941

Similar proteinsi

Entry informationi

Entry nameiDYR_HAEIN
AccessioniPrimary (citable) accession number: P43791
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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