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P43791 (DYR_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Synonyms:folH
Ordered Locus Names:HI_0899
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length160 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Miscellaneous

Isolates R906, R1042, and R1047 are trimethoprim-resistant.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 160160Dihydrofolate reductase
PRO_0000186391

Regions

Domain2 – 159158DHFR
Nucleotide binding14 – 207NADP By similarity
Nucleotide binding46 – 472NADP By similarity
Nucleotide binding64 – 652NADP By similarity
Nucleotide binding96 – 1038NADP By similarity

Sites

Binding site61Substrate; via carbonyl oxygen By similarity
Binding site81NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site281Substrate By similarity
Binding site531Substrate By similarity
Binding site581Substrate By similarity
Binding site1141Substrate By similarity

Natural variations

Natural variant131N → S in strain: Isolate R1042 and Isolate R1047.
Natural variant211M → I in strain: Isolate R1047.
Natural variant541P → A in strain: Isolate R1047.
Natural variant561P → A in strain: Isolate R906.
Natural variant671L → P in strain: Isolate R1042.
Natural variant691E → K in strain: Isolate R1042.
Natural variant741I → V in strain: Isolate R1042.
Natural variant771D → N in strain: Isolate R1042.
Natural variant791F → L in strain: Isolate R1042.
Natural variant951I → L in strain: Isolate R1047.
Natural variant1351E → K in strain: Isolate R1042.
Natural variant1421R → H in strain: Isolate R1042.
Natural variant1541F → S in strain: Isolate R1042.

Sequences

Sequence LengthMass (Da)Tools
P43791 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 384A1D64092B8C4F

FASTA16018,913
        10         20         30         40         50         60 
MTFSLIVATT LNNVIGKDNQ MPWHLPADLA WFRQNTTGKP VIMGRKTFES IGRPLPKRTN 

        70         80         90        100        110        120 
IVLSRQLFEH EGVIWKDSFE SAVNFVRDFD EIMLIGGGEL FKQYLPKADK LYLTQIQTEL 

       130        140        150        160 
DGDTFFPQLN WEEWEIEFDE YRKADEQNRY DCRFLILTRK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22559.1.
X84207 Genomic DNA. Translation: CAA58993.1.
X84205 Genomic DNA. Translation: CAA58991.1.
X84206 Genomic DNA. Translation: CAA58992.1.
PIRC64101.
S52336.
S52337.
S52338.
RefSeqNP_439060.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43791.
SMRP43791. Positions 3-160.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0899.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22559; AAC22559; HI_0899.
GeneID949905.
KEGGhin:HI0899.
PATRIC20190455. VBIHaeInf48452_0941.

Phylogenomic databases

eggNOGCOG0262.
KOK00287.
OMADTQFPDW.
OrthoDBEOG6KT2V2.
ProtClustDBCLSK870655.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_HAEIN
AccessionPrimary (citable) accession number: P43791
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names