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Protein

Dihydrofolate reductase

Gene

folA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei6 – 61Substrate; via carbonyl oxygenBy similarity
Binding sitei8 – 81NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei28 – 281SubstrateBy similarity
Binding sitei53 – 531SubstrateBy similarity
Binding sitei58 – 581SubstrateBy similarity
Binding sitei114 – 1141SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 207NADPBy similarity
Nucleotide bindingi46 – 472NADPBy similarity
Nucleotide bindingi64 – 652NADPBy similarity
Nucleotide bindingi96 – 1038NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
  5. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:folH
Ordered Locus Names:HI_0899
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160Dihydrofolate reductasePRO_0000186391Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0899.

Structurei

3D structure databases

ProteinModelPortaliP43791.
SMRiP43791. Positions 3-160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 159158DHFRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
KOiK00287.
OMAiDTQFPDW.
OrthoDBiEOG6KT2V2.
PhylomeDBiP43791.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43791-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTFSLIVATT LNNVIGKDNQ MPWHLPADLA WFRQNTTGKP VIMGRKTFES
60 70 80 90 100
IGRPLPKRTN IVLSRQLFEH EGVIWKDSFE SAVNFVRDFD EIMLIGGGEL
110 120 130 140 150
FKQYLPKADK LYLTQIQTEL DGDTFFPQLN WEEWEIEFDE YRKADEQNRY
160
DCRFLILTRK
Length:160
Mass (Da):18,913
Last modified:November 1, 1995 - v1
Checksum:i384A1D64092B8C4F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131N → S in strain: Isolate R1042 and Isolate R1047.
Natural varianti21 – 211M → I in strain: Isolate R1047.
Natural varianti54 – 541P → A in strain: Isolate R1047.
Natural varianti56 – 561P → A in strain: Isolate R906.
Natural varianti67 – 671L → P in strain: Isolate R1042.
Natural varianti69 – 691E → K in strain: Isolate R1042.
Natural varianti74 – 741I → V in strain: Isolate R1042.
Natural varianti77 – 771D → N in strain: Isolate R1042.
Natural varianti79 – 791F → L in strain: Isolate R1042.
Natural varianti95 – 951I → L in strain: Isolate R1047.
Natural varianti135 – 1351E → K in strain: Isolate R1042.
Natural varianti142 – 1421R → H in strain: Isolate R1042.
Natural varianti154 – 1541F → S in strain: Isolate R1042.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22559.1.
X84207 Genomic DNA. Translation: CAA58993.1.
X84205 Genomic DNA. Translation: CAA58991.1.
X84206 Genomic DNA. Translation: CAA58992.1.
PIRiC64101.
S52336.
S52337.
S52338.
RefSeqiNP_439060.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22559; AAC22559; HI_0899.
GeneIDi949905.
KEGGihin:HI0899.
PATRICi20190455. VBIHaeInf48452_0941.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22559.1.
X84207 Genomic DNA. Translation: CAA58993.1.
X84205 Genomic DNA. Translation: CAA58991.1.
X84206 Genomic DNA. Translation: CAA58992.1.
PIRiC64101.
S52336.
S52337.
S52338.
RefSeqiNP_439060.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43791.
SMRiP43791. Positions 3-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22559; AAC22559; HI_0899.
GeneIDi949905.
KEGGihin:HI0899.
PATRICi20190455. VBIHaeInf48452_0941.

Phylogenomic databases

eggNOGiCOG0262.
KOiK00287.
OMAiDTQFPDW.
OrthoDBiEOG6KT2V2.
PhylomeDBiP43791.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. de Groot R.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Isolate R1042, Isolate R1047 and Isolate R906.

Entry informationi

Entry nameiDYR_HAEIN
AccessioniPrimary (citable) accession number: P43791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolates R906, R1042, and R1047 are trimethoprim-resistant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.