Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydrofolate reductase

Gene

folA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei6Substrate; via carbonyl oxygenBy similarity1
Binding sitei8NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei28SubstrateBy similarity1
Binding sitei53SubstrateBy similarity1
Binding sitei58SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 20NADPBy similarity7
Nucleotide bindingi46 – 47NADPBy similarity2
Nucleotide bindingi64 – 65NADPBy similarity2
Nucleotide bindingi96 – 103NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:folH
Ordered Locus Names:HI_0899
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863911 – 160Dihydrofolate reductaseAdd BLAST160

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0899.

Structurei

3D structure databases

ProteinModelPortaliP43791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 159DHFRPROSITE-ProRule annotationAdd BLAST158

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YYV. Bacteria.
COG0262. LUCA.
KOiK00287.
OMAiDTQFPDW.
PhylomeDBiP43791.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFSLIVATT LNNVIGKDNQ MPWHLPADLA WFRQNTTGKP VIMGRKTFES
60 70 80 90 100
IGRPLPKRTN IVLSRQLFEH EGVIWKDSFE SAVNFVRDFD EIMLIGGGEL
110 120 130 140 150
FKQYLPKADK LYLTQIQTEL DGDTFFPQLN WEEWEIEFDE YRKADEQNRY
160
DCRFLILTRK
Length:160
Mass (Da):18,913
Last modified:November 1, 1995 - v1
Checksum:i384A1D64092B8C4F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti13N → S in strain: Isolate R1042 and Isolate R1047. 1
Natural varianti21M → I in strain: Isolate R1047. 1
Natural varianti54P → A in strain: Isolate R1047. 1
Natural varianti56P → A in strain: Isolate R906. 1
Natural varianti67L → P in strain: Isolate R1042. 1
Natural varianti69E → K in strain: Isolate R1042. 1
Natural varianti74I → V in strain: Isolate R1042. 1
Natural varianti77D → N in strain: Isolate R1042. 1
Natural varianti79F → L in strain: Isolate R1042. 1
Natural varianti95I → L in strain: Isolate R1047. 1
Natural varianti135E → K in strain: Isolate R1042. 1
Natural varianti142R → H in strain: Isolate R1042. 1
Natural varianti154F → S in strain: Isolate R1042. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22559.1.
X84207 Genomic DNA. Translation: CAA58993.1.
X84205 Genomic DNA. Translation: CAA58991.1.
X84206 Genomic DNA. Translation: CAA58992.1.
PIRiC64101.
S52336.
S52337.
S52338.
RefSeqiNP_439060.1. NC_000907.1.
WP_005648125.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22559; AAC22559; HI_0899.
GeneIDi949905.
KEGGihin:HI0899.
PATRICi20190455. VBIHaeInf48452_0941.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22559.1.
X84207 Genomic DNA. Translation: CAA58993.1.
X84205 Genomic DNA. Translation: CAA58991.1.
X84206 Genomic DNA. Translation: CAA58992.1.
PIRiC64101.
S52336.
S52337.
S52338.
RefSeqiNP_439060.1. NC_000907.1.
WP_005648125.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43791.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22559; AAC22559; HI_0899.
GeneIDi949905.
KEGGihin:HI0899.
PATRICi20190455. VBIHaeInf48452_0941.

Phylogenomic databases

eggNOGiENOG4108YYV. Bacteria.
COG0262. LUCA.
KOiK00287.
OMAiDTQFPDW.
PhylomeDBiP43791.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYR_HAEIN
AccessioniPrimary (citable) accession number: P43791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolates R906, R1042, and R1047 are trimethoprim-resistant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.