Reviewed, UniProtKB/Swiss-Prot P43783 (GSHR_HAEIN)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:	gor
Ordered Locus Names:	HI0161

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Glutathione reductase		PRO_0000067976
Regions
	Nucleotide binding	34 – 42	9	FAD By similarity
Sites
	Active site	445	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	42 ↔ 47		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43783-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 846988C215FE949A	FASTA	456	49,329
	10 20 30 40 50 60 MTKHYDYIAI GGGSGGIASL NRAASYGKKC AIIEAKHLGG TCVNVGCVPK KVMFYGAHIA 70 80 90 100 110 120 EAINNYAPDY GFDVEVKKFD FSKLIESRQA YISRIHTSYN NVLAKNNIDV INGFGKFVDA 130 140 150 160 170 180 HTIEVTLADG TKEQVTADHI LIATGGRPYR PNIKGQEYGI DSDGFFALTE LPKRAAVIGA 190 200 210 220 230 240 GYIAVELSGV LNSLGVETHL LVRRHAPMRN QDPLIVETLV EVLAQDGIQL HTNSTPSEIV 250 260 270 280 290 300 KNADGSLTVR CDGQSDVTVD CVIWAAGRVP TTDKIGLENA GVETNEHGYV KVDKYQNTNV 310 320 330 340 350 360 KGIYAVGDII ENGIELTPVA VAAGRRLSER LFNNKPTEYL DYSLVPTVVF SHPPIGTVGL 370 380 390 400 410 420 TEPQAIEQYG AENVKVYKSS FTAMYTAVTQ HRQPCKMKLV CVGKDEKVVG LHGIGFGVDE 430 440 450 MIQGFAVAIK MGATKADFDN TVAIHPTGSE EFVTMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Barcak G.J., Heimer S.R. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U20229 Genomic DNA. Translation: AAA62137.1. L42023 Genomic DNA. Translation: AAC21833.1.
PIR	A64052.
RefSeq	NP_438331.1.
3D structure databases
HSSP	HSSP built from PDB template 1ALG based on UniProtKB P00390.
SMR	P43783. Positions 2-456.
ModBase	Search...
Genome annotation databases
GeneID	951071.
GenomeReviews	Gene locus HI0161 in contig L42023_GR.
KEGG	hin:HI0161.
NMPDR	fig|71421.1.peg.152.
TIGR	HI0161.
Phylogenomic databases
HOGENOM	P43783.
OMA	P43783. HRQPCKM.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0161-MON.
BRENDA	1.8.1.7. 109.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glut_reduct_1. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01421. gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_HAEIN

Accession

Primary (citable) accession number: P43783

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)