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Protein

ATP-dependent protease ATPase subunit HslU

Gene

hslU

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18ATP; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei257ATPBy similarity1
Binding sitei322ATPBy similarity1
Binding sitei394ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 65ATP1 Publication6
Nucleotide bindingi306 – 309ATP1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent protease ATPase subunit HslU
Alternative name(s):
Unfoldase HslU
Gene namesi
Name:hslU
Ordered Locus Names:HI_0497
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001605081 – 444ATP-dependent protease ATPase subunit HslUAdd BLAST444

Interactioni

Subunit structurei

A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
hslVP437724EBI-1030296,EBI-1030290

Protein-protein interaction databases

DIPiDIP-6175N.
IntActiP43773. 1 interactor.
STRINGi71421.HI0497.

Structurei

Secondary structure

1444
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 14Combined sources9
Helixi21 – 39Combined sources19
Turni42 – 47Combined sources6
Beta strandi53 – 56Combined sources4
Beta strandi58 – 62Combined sources5
Helixi63 – 73Combined sources11
Beta strandi78 – 82Combined sources5
Helixi83 – 86Combined sources4
Helixi92 – 94Combined sources3
Helixi97 – 117Combined sources21
Beta strandi123 – 128Combined sources6
Helixi228 – 230Combined sources3
Beta strandi231 – 234Combined sources4
Helixi237 – 251Combined sources15
Beta strandi253 – 257Combined sources5
Helixi259 – 262Combined sources4
Beta strandi269 – 271Combined sources3
Helixi272 – 287Combined sources16
Beta strandi290 – 293Combined sources4
Beta strandi296 – 299Combined sources4
Helixi300 – 302Combined sources3
Beta strandi304 – 309Combined sources6
Beta strandi312 – 314Combined sources3
Helixi316 – 318Combined sources3
Helixi321 – 324Combined sources4
Beta strandi329 – 332Combined sources4
Helixi338 – 346Combined sources9
Helixi352 – 361Combined sources10
Turni362 – 364Combined sources3
Beta strandi366 – 369Combined sources4
Helixi371 – 387Combined sources17
Helixi392 – 394Combined sources3
Helixi395 – 410Combined sources16
Helixi411 – 413Combined sources3
Beta strandi418 – 421Combined sources4
Helixi423 – 430Combined sources8
Turni431 – 435Combined sources5
Helixi437 – 443Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G3IX-ray3.41A/B/C/D/E/F/S/T/U/V/W/X1-444[»]
1G41X-ray2.30A1-444[»]
1IM2X-ray2.80A1-444[»]
1KYIX-ray3.10A/B/C/D/E/F/S/T/U/V/W/X1-444[»]
1OFHX-ray2.50A/B/C1-107[»]
A/B/C244-444[»]
1OFIX-ray3.20A/B/C1-107[»]
A/B/C244-444[»]
ProteinModelPortaliP43773.
SMRiP43773.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43773.

Family & Domainsi

Sequence similaritiesi

Belongs to the ClpX chaperone family. HslU subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C4N. Bacteria.
COG1220. LUCA.
KOiK03667.
OMAiMGGRTKK.
PhylomeDBiP43773.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00249. HslU. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004491. HslU.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11262:SF3. PTHR11262:SF3. 2 hits.
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00390. hslU. 1 hit.

Sequencei

Sequence statusi: Complete.

P43773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEMTPREIV SELDQHIIGQ ADAKRAVAIA LRNRWRRMQL QEPLRHEVTP
60 70 80 90 100
KNILMIGPTG VGKTEIARRL AKLANAPFIK VEATKFTEVG YVGKEVDSII
110 120 130 140 150
RDLTDSAMKL VRQQEIAKNR ARAEDVAEER ILDALLPPAK NQWGEVENHD
160 170 180 190 200
SHSSTRQAFR KKLREGQLDD KEIEIDVSAG VSMGVEIMAP PGMEEMTNQL
210 220 230 240 250
QSLFQNLGSD KTKKRKMKIK DALKALIDDE AAKLINPEEL KQKAIDAVEQ
260 270 280 290 300
NGIVFIDEID KICKKGEYSG ADVSREGVQR DLLPLVEGST VSTKHGMVKT
310 320 330 340 350
DHILFIASGA FQVARPSDLI PELQGRLPIR VELTALSAAD FERILTEPHA
360 370 380 390 400
SLTEQYKALM ATEGVNIAFT TDAVKKIAEA AFRVNEKTEN IGARRLHTVM
410 420 430 440
ERLMDKISFS ASDMNGQTVN IDAAYVADAL GEVVENEDLS RFIL
Length:444
Mass (Da):49,372
Last modified:November 1, 1995 - v1
Checksum:iC346EA478E4094CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22154.1.
RefSeqiNP_438655.1. NC_000907.1.
WP_010868991.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22154; AAC22154; HI_0497.
GeneIDi950614.
KEGGihin:HI0497.
PATRICi20189545. VBIHaeInf48452_0515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22154.1.
RefSeqiNP_438655.1. NC_000907.1.
WP_010868991.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G3IX-ray3.41A/B/C/D/E/F/S/T/U/V/W/X1-444[»]
1G41X-ray2.30A1-444[»]
1IM2X-ray2.80A1-444[»]
1KYIX-ray3.10A/B/C/D/E/F/S/T/U/V/W/X1-444[»]
1OFHX-ray2.50A/B/C1-107[»]
A/B/C244-444[»]
1OFIX-ray3.20A/B/C1-107[»]
A/B/C244-444[»]
ProteinModelPortaliP43773.
SMRiP43773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6175N.
IntActiP43773. 1 interactor.
STRINGi71421.HI0497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22154; AAC22154; HI_0497.
GeneIDi950614.
KEGGihin:HI0497.
PATRICi20189545. VBIHaeInf48452_0515.

Phylogenomic databases

eggNOGiENOG4105C4N. Bacteria.
COG1220. LUCA.
KOiK03667.
OMAiMGGRTKK.
PhylomeDBiP43773.

Miscellaneous databases

EvolutionaryTraceiP43773.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00249. HslU. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004491. HslU.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11262:SF3. PTHR11262:SF3. 2 hits.
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00390. hslU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHSLU_HAEIN
AccessioniPrimary (citable) accession number: P43773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.