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Protein

ATP-dependent protease subunit HslV

Gene

hslV

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.

Catalytic activityi

ATP-dependent cleavage of peptide bonds with broad specificity.UniRule annotation

Enzyme regulationi

Allosterically activated by HslU binding.UniRule annotation1 Publication

Kineticsi

Arc-H(6)-SulA(CT) is a construct consisting of the Arc repressor protein fused to 6 His residues and the 11 carboxy terminal residues of SulA.

  1. KM=2.3 µM for Arc-H(6)-SulA(CT)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21
    Metal bindingi158 – 1581Sodium; via carbonyl oxygen
    Metal bindingi161 – 1611Sodium; via carbonyl oxygen
    Metal bindingi164 – 1641Sodium; via carbonyl oxygen

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Keywords - Ligandi

    Metal-binding, Sodium

    Protein family/group databases

    MEROPSiT01.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent protease subunit HslVUniRule annotation (EC:3.4.25.2UniRule annotation)
    Gene namesi
    Name:hslVUniRule annotation
    Ordered Locus Names:HI_0496
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    Proteomesi
    • UP000000579 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemovedBy similarity
    Chaini2 – 175174ATP-dependent protease subunit HslVPRO_0000148111Add
    BLAST

    Interactioni

    Subunit structurei

    A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.UniRule annotation4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    hslUP437734EBI-1030290,EBI-1030296

    Protein-protein interaction databases

    DIPiDIP-6176N.
    IntActiP43772. 1 interaction.
    STRINGi71421.HI0496.

    Structurei

    Secondary structure

    1
    175
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96Combined sources
    Beta strandi12 – 176Combined sources
    Beta strandi21 – 233Combined sources
    Beta strandi26 – 305Combined sources
    Beta strandi35 – 384Combined sources
    Turni39 – 424Combined sources
    Beta strandi43 – 497Combined sources
    Helixi51 – 6717Combined sources
    Turni68 – 703Combined sources
    Helixi72 – 8514Combined sources
    Helixi89 – 913Combined sources
    Beta strandi95 – 995Combined sources
    Beta strandi104 – 1085Combined sources
    Turni109 – 1113Combined sources
    Beta strandi112 – 1143Combined sources
    Beta strandi121 – 1255Combined sources
    Helixi128 – 14114Combined sources
    Helixi146 – 16015Combined sources
    Beta strandi169 – 1735Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G3IX-ray3.41G/H/I/J/K/L/M/N/O/P/Q/R2-175[»]
    1G3KX-ray1.90A/B/C2-175[»]
    1JJWX-ray1.90A/B/C2-175[»]
    1KYIX-ray3.10G/H/I/J/K/L/M/N/O/P/Q/R2-175[»]
    1OFHX-ray2.50G/H/I/L/M/N2-175[»]
    1OFIX-ray3.20G/H/I/L/M/N2-175[»]
    ProteinModelPortaliP43772.
    SMRiP43772. Positions 2-174.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43772.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family. HslV subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4108R5P. Bacteria.
    COG5405. LUCA.
    KOiK01419.
    OMAiIMKGNAR.
    PhylomeDBiP43772.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00248. HslV. 1 hit.
    InterProiIPR022281. ATP-dep_Prtase_HsIV_su.
    IPR029055. Ntn_hydrolases_N.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PIRSFiPIRSF039093. HslV. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03692. ATP_dep_HslV. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43772-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTTIVSVRRN GQVVVGGDGQ VSLGNTVMKG NARKVRRLYN GKVLAGFAGG
    60 70 80 90 100
    TADAFTLFEL FERKLEMHQG HLLKSAVELA KDWRTDRALR KLEAMLIVAD
    110 120 130 140 150
    EKESLIITGI GDVVQPEEDQ ILAIGSGGNY ALSAARALVE NTELSAHEIV
    160 170
    EKSLRIAGDI CVFTNTNFTI EELPN
    Length:175
    Mass (Da):19,012
    Last modified:January 23, 2007 - v2
    Checksum:iCEA1C77B7AB2724C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L42023 Genomic DNA. Translation: AAC22153.1.
    PIRiC64072.
    RefSeqiNP_438654.1. NC_000907.1.
    WP_005649427.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22153; AAC22153; HI_0496.
    GeneIDi949571.
    KEGGihin:HI0496.
    PATRICi20189543. VBIHaeInf48452_0514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L42023 Genomic DNA. Translation: AAC22153.1.
    PIRiC64072.
    RefSeqiNP_438654.1. NC_000907.1.
    WP_005649427.1. NC_000907.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G3IX-ray3.41G/H/I/J/K/L/M/N/O/P/Q/R2-175[»]
    1G3KX-ray1.90A/B/C2-175[»]
    1JJWX-ray1.90A/B/C2-175[»]
    1KYIX-ray3.10G/H/I/J/K/L/M/N/O/P/Q/R2-175[»]
    1OFHX-ray2.50G/H/I/L/M/N2-175[»]
    1OFIX-ray3.20G/H/I/L/M/N2-175[»]
    ProteinModelPortaliP43772.
    SMRiP43772. Positions 2-174.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-6176N.
    IntActiP43772. 1 interaction.
    STRINGi71421.HI0496.

    Protein family/group databases

    MEROPSiT01.006.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC22153; AAC22153; HI_0496.
    GeneIDi949571.
    KEGGihin:HI0496.
    PATRICi20189543. VBIHaeInf48452_0514.

    Phylogenomic databases

    eggNOGiENOG4108R5P. Bacteria.
    COG5405. LUCA.
    KOiK01419.
    OMAiIMKGNAR.
    PhylomeDBiP43772.

    Miscellaneous databases

    EvolutionaryTraceiP43772.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00248. HslV. 1 hit.
    InterProiIPR022281. ATP-dep_Prtase_HsIV_su.
    IPR029055. Ntn_hydrolases_N.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PIRSFiPIRSF039093. HslV. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03692. ATP_dep_HslV. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHSLV_HAEIN
    AccessioniPrimary (citable) accession number: P43772
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: September 7, 2016
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.