Ribonucleoside-diphosphate reductase subunit alpha

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdA
Ordered Locus Names:	HI1659

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

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Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H₂O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 1 ATP-cone domain.

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Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 756

756

Ribonucleoside-diphosphate reductase subunit alpha

PRO_0000187214

Regions

Domain

5 – 95

91

ATP-cone

Sites

Active site

225

1

Hydrogen atom transfer By similarity

Active site

437

1

Proton acceptor By similarity

Active site

439

1

Proton acceptor By similarity

Active site

441

1

Proton acceptor By similarity

Active site

462

1

Hydrogen atom transfer By similarity

Active site

730

1

Electron transfer By similarity

Active site

731

1

Electron transfer By similarity

Site

232

1

Allosteric effector binding By similarity

Site

262

1

Allosteric effector binding By similarity

Site

749

1

Interacts with thioredoxin/glutaredoxin By similarity

Site

754

1

Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond

225 ↔ 462

Redox-active By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P43754-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 839D02FA61E185D3
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FASTA

756

85,696

        10         20         30         40         50         60 
MNKSLMVTKR DGTQEQINLD KIHRVITWAA EGLDNVSVSQ VELRSHIQFY EGIRTSDIHE 

        70         80         90        100        110        120 
TIIKAAADLI SKDSPDYQYL AARLAIFHLR KKAYGHFDPP RLYDHVKKLV RMEKYDQALL 

       130        140        150        160        170        180 
DDYTREEWDT MDGFIDHWRD MTFSYAAVKQ LEGKYLVQNR VTGEIYESAQ FLYLLVSASL 

       190        200        210        220        230        240 
FSKYPKETRL DYVKRFYDAT STFKISLPTP IMAGVRTPTR QFSSCVLIEC DDSLDSINAT 

       250        260        270        280        290        300 
ASAIVKYVSQ RAGIGINAGA IRALGSEIRG GEAFHTGCIP FYKYFQTAVK SCSQGGVRGG 

       310        320        330        340        350        360 
AATLYYPIWH LEAENLLVLK NNRGVEDNRV RHMDYGVQLN KLMYQRLIKG SEITLFSPSD 

       370        380        390        400        410        420 
VPGLYEAFFA DQDKFEELYV KYEQDPTIRK RTVKAVEIFS LLMQERASTG RIYIQNVDHC 

       430        440        450        460        470        480 
NTHSPFDPQV APVRQSNLCL EIALPTKPLQ HINDENGEIA LCTLSAFNLG KIENLDELEE 

       490        500        510        520        530        540 
LADLAVRSLD ALLDYQDYPV VAAKRSSLAR RSLGIGVINY AYYLAKNGVR YSDGSANDLT 

       550        560        570        580        590        600 
HRTFEAIQYY LLKASMNLAK EQGACEYFNE TTYAKGILPI DTYKKDLDSL TQEPLHYDWE 

       610        620        630        640        650        660 
SLRKDIQEFG LRNSTLTALM PSETSSQISN ATNGIEPPRG HVSIKASKDG ILKQVVPEYE 

       670        680        690        700        710        720 
NLMDNYELLW DIPSNDGYLH LVGIMQKFVD QAISANTNYD PKRFEDGKVP MKVLLKDLLT 

       730        740        750 
AYKYGLKTLY YQNTRDGAED VQEDLDDGCA GGACKI

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References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd."
Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I.

Venter J.C.
Science 269:496-512(1995) [PubMed: 7542800] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

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Cross-references

Sequence databases
	L42023 Genomic DNA. Translation: AAC23305.1. Different initiation.
PIR	B64135.
RefSeq	NP_439801.2.
3D structure databases
HSSP	HSSP built from PDB template 5R1R based on UniProtKB P00452.
SMR	P43754. Positions 4-737.
ModBase	Search...
Genome annotation databases
GeneID	950495.
GenomeReviews	Gene locus HI1659 in contig L42023_GR.
KEGG	hin:HI1659.
NMPDR	fig\|71421.1.peg.1573.
TIGR	HI1659.
Phylogenomic databases
HOGENOM	P43754.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1659-MON.
BRENDA	1.17.4.1. 109.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF03477. ATP-cone. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS51161. ATP_CONE. 1 hit. PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

RIR1_HAEIN

Accession

Primary (citable) accession number: P43754

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents