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P43754

- RIR1_HAEIN

UniProt

P43754 - RIR1_HAEIN

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene
nrdA, HI_1659
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the alpha subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Allosteric activator By similarity
Binding sitei55 – 551Allosteric activator By similarity
Binding sitei91 – 911Allosteric activator By similarity
Binding sitei209 – 2091Substrate By similarity
Sitei225 – 2251Important for hydrogen atom transfer By similarity
Sitei232 – 2321Allosteric effector binding By similarity
Binding sitei253 – 2531Substrate; via amide nitrogen By similarity
Sitei262 – 2621Allosteric effector binding By similarity
Active sitei437 – 4371Proton acceptor By similarity
Active sitei439 – 4391Cysteine radical intermediate By similarity
Active sitei441 – 4411Proton acceptor By similarity
Sitei462 – 4621Important for hydrogen atom transfer By similarity
Sitei730 – 7301Important for electron transfer By similarity
Sitei731 – 7311Important for electron transfer By similarity
Sitei749 – 7491Interacts with thioredoxin/glutaredoxin By similarity
Sitei754 – 7541Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:HI_1659
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 756756Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi225 ↔ 462Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP43754.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi71421.HI1659.

Structurei

3D structure databases

ProteinModelPortaliP43754.
SMRiP43754. Positions 5-737.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 9591ATP-coneAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 217Allosteric activator binding By similarity
Regioni224 – 2252Substrate binding By similarity
Regioni437 – 4415Substrate binding By similarity
Regioni621 – 6255Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Phylogenomic databases

eggNOGiCOG0209.
KOiK00525.
OMAiLLWQMPS.
OrthoDBiEOG6J48HC.
PhylomeDBiP43754.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43754-1 [UniParc]FASTAAdd to Basket

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MNKSLMVTKR DGTQEQINLD KIHRVITWAA EGLDNVSVSQ VELRSHIQFY    50
EGIRTSDIHE TIIKAAADLI SKDSPDYQYL AARLAIFHLR KKAYGHFDPP 100
RLYDHVKKLV RMEKYDQALL DDYTREEWDT MDGFIDHWRD MTFSYAAVKQ 150
LEGKYLVQNR VTGEIYESAQ FLYLLVSASL FSKYPKETRL DYVKRFYDAT 200
STFKISLPTP IMAGVRTPTR QFSSCVLIEC DDSLDSINAT ASAIVKYVSQ 250
RAGIGINAGA IRALGSEIRG GEAFHTGCIP FYKYFQTAVK SCSQGGVRGG 300
AATLYYPIWH LEAENLLVLK NNRGVEDNRV RHMDYGVQLN KLMYQRLIKG 350
SEITLFSPSD VPGLYEAFFA DQDKFEELYV KYEQDPTIRK RTVKAVEIFS 400
LLMQERASTG RIYIQNVDHC NTHSPFDPQV APVRQSNLCL EIALPTKPLQ 450
HINDENGEIA LCTLSAFNLG KIENLDELEE LADLAVRSLD ALLDYQDYPV 500
VAAKRSSLAR RSLGIGVINY AYYLAKNGVR YSDGSANDLT HRTFEAIQYY 550
LLKASMNLAK EQGACEYFNE TTYAKGILPI DTYKKDLDSL TQEPLHYDWE 600
SLRKDIQEFG LRNSTLTALM PSETSSQISN ATNGIEPPRG HVSIKASKDG 650
ILKQVVPEYE NLMDNYELLW DIPSNDGYLH LVGIMQKFVD QAISANTNYD 700
PKRFEDGKVP MKVLLKDLLT AYKYGLKTLY YQNTRDGAED VQEDLDDGCA 750
GGACKI 756
Length:756
Mass (Da):85,696
Last modified:November 1, 1995 - v1
Checksum:i839D02FA61E185D3
GO

Sequence cautioni

The sequence AAC23305.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC23305.1. Different initiation.
PIRiB64135.
RefSeqiNP_439801.2. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23305; AAC23305; HI_1659.
GeneIDi950495.
KEGGihin:HI1659.
PATRICi20192067. VBIHaeInf48452_1737.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC23305.1 . Different initiation.
PIRi B64135.
RefSeqi NP_439801.2. NC_000907.1.

3D structure databases

ProteinModelPortali P43754.
SMRi P43754. Positions 5-737.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1659.

Proteomic databases

PRIDEi P43754.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC23305 ; AAC23305 ; HI_1659 .
GeneIDi 950495.
KEGGi hin:HI1659.
PATRICi 20192067. VBIHaeInf48452_1737.

Phylogenomic databases

eggNOGi COG0209.
KOi K00525.
OMAi LLWQMPS.
OrthoDBi EOG6J48HC.
PhylomeDBi P43754.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiRIR1_HAEIN
AccessioniPrimary (citable) accession number: P43754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi