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P43751

- PFLA_HAEIN

UniProt

P43751 - PFLA_HAEIN

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Protein
Pyruvate formate-lyase 1-activating enzyme
Gene
pflA, act, HI_0179
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Synonyms:act
Ordered Locus Names:HI_0179
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 246245Pyruvate formate-lyase 1-activating enzyme
PRO_0000200525Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0179.

Structurei

3D structure databases

ProteinModelPortaliP43751.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
KOiK04069.
OMAiQNLIGVP.
OrthoDBiEOG64FKHC.
PhylomeDBiP43751.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43751-1 [UniParc]FASTAAdd to Basket

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MSVLGRIHSF ESCGTVDGPG IRFILFMQGC LMRCKYCHNR DTWDLEGGKE    50
ISVEDLMKEV VTYRHFMNAT GGGVTASGGE AVLQAEFVRD WFRACKEEGI 100
NTCLDTNGFV RHYDHIIDEL LDVTDLVLLD LKELNDQVHQ NLIGVPNKRT 150
LEFAKYLQKR NQHTWIRYVV VPGYTDSDHD VHLLGQFIEG MTNIEKVELL 200
PYHRLGVHKW KTLGLDYELE NVLPPTKESL EHIKTILEGY GHTVKF 246
Length:246
Mass (Da):28,235
Last modified:January 23, 2007 - v2
Checksum:i884313BD5B84376F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC21848.1.
PIRiE64052.
RefSeqiNP_438347.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21848; AAC21848; HI_0179.
GeneIDi951086.
KEGGihin:HI0179.
PATRICi20188853. VBIHaeInf48452_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC21848.1 .
PIRi E64052.
RefSeqi NP_438347.1. NC_000907.1.

3D structure databases

ProteinModelPortali P43751.
ModBasei Search...

Protein-protein interaction databases

STRINGi 71421.HI0179.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC21848 ; AAC21848 ; HI_0179 .
GeneIDi 951086.
KEGGi hin:HI0179.
PATRICi 20188853. VBIHaeInf48452_0183.

Phylogenomic databases

eggNOGi COG1180.
KOi K04069.
OMAi QNLIGVP.
OrthoDBi EOG64FKHC.
PhylomeDBi P43751.

Family and domain databases

InterProi IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiPFLA_HAEIN
AccessioniPrimary (citable) accession number: P43751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi