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P43751 (PFLA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase 1-activating enzyme

EC=1.97.1.4
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene names
Name:pflA
Synonyms:act
Ordered Locus Names:HI_0179
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 246245Pyruvate formate-lyase 1-activating enzyme
PRO_0000200525

Sites

Metal binding301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding341Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding371Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
P43751 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 884313BD5B84376F

FASTA24628,235
        10         20         30         40         50         60 
MSVLGRIHSF ESCGTVDGPG IRFILFMQGC LMRCKYCHNR DTWDLEGGKE ISVEDLMKEV 

        70         80         90        100        110        120 
VTYRHFMNAT GGGVTASGGE AVLQAEFVRD WFRACKEEGI NTCLDTNGFV RHYDHIIDEL 

       130        140        150        160        170        180 
LDVTDLVLLD LKELNDQVHQ NLIGVPNKRT LEFAKYLQKR NQHTWIRYVV VPGYTDSDHD 

       190        200        210        220        230        240 
VHLLGQFIEG MTNIEKVELL PYHRLGVHKW KTLGLDYELE NVLPPTKESL EHIKTILEGY 


GHTVKF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC21848.1.
PIRE64052.
RefSeqNP_438347.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43751.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC21848; AAC21848; HI_0179.
GeneID951086.
KEGGhin:HI0179.
PATRIC20188853. VBIHaeInf48452_0183.

Phylogenomic databases

eggNOGCOG1180.
KOK04069.
OMAQNLIGVP.
OrthoDBEOG64FKHC.
PhylomeDBP43751.

Family and domain databases

InterProIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsTIGR02493. PFLA. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFLA_HAEIN
AccessionPrimary (citable) accession number: P43751
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names