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P43751

- PFLA_HAEIN

UniProt

P43751 - PFLA_HAEIN

Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
    Alternative name(s):
    Formate-C-acetyltransferase-activating enzyme 1
    PFL-activating enzyme 1
    Gene namesi
    Name:pflA
    Synonyms:act
    Ordered Locus Names:HI_0179
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 246245Pyruvate formate-lyase 1-activating enzymePRO_0000200525Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0179.

    Structurei

    3D structure databases

    ProteinModelPortaliP43751.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1180.
    KOiK04069.
    OMAiQNLIGVP.
    OrthoDBiEOG64FKHC.
    PhylomeDBiP43751.

    Family and domain databases

    InterProiIPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02493. PFLA. 1 hit.
    PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43751-1 [UniParc]FASTAAdd to Basket

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    MSVLGRIHSF ESCGTVDGPG IRFILFMQGC LMRCKYCHNR DTWDLEGGKE    50
    ISVEDLMKEV VTYRHFMNAT GGGVTASGGE AVLQAEFVRD WFRACKEEGI 100
    NTCLDTNGFV RHYDHIIDEL LDVTDLVLLD LKELNDQVHQ NLIGVPNKRT 150
    LEFAKYLQKR NQHTWIRYVV VPGYTDSDHD VHLLGQFIEG MTNIEKVELL 200
    PYHRLGVHKW KTLGLDYELE NVLPPTKESL EHIKTILEGY GHTVKF 246
    Length:246
    Mass (Da):28,235
    Last modified:January 23, 2007 - v2
    Checksum:i884313BD5B84376F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21848.1.
    PIRiE64052.
    RefSeqiNP_438347.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC21848; AAC21848; HI_0179.
    GeneIDi951086.
    KEGGihin:HI0179.
    PATRICi20188853. VBIHaeInf48452_0183.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21848.1 .
    PIRi E64052.
    RefSeqi NP_438347.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P43751.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0179.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC21848 ; AAC21848 ; HI_0179 .
    GeneIDi 951086.
    KEGGi hin:HI0179.
    PATRICi 20188853. VBIHaeInf48452_0183.

    Phylogenomic databases

    eggNOGi COG1180.
    KOi K04069.
    OMAi QNLIGVP.
    OrthoDBi EOG64FKHC.
    PhylomeDBi P43751.

    Family and domain databases

    InterProi IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02493. PFLA. 1 hit.
    PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiPFLA_HAEIN
    AccessioniPrimary (citable) accession number: P43751
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3