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Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi34 – 341Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi37 – 371Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Synonyms:act
Ordered Locus Names:HI_0179
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 246245Pyruvate formate-lyase 1-activating enzymePRO_0000200525Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0179.

Structurei

3D structure databases

ProteinModelPortaliP43751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
KOiK04069.
OMAiCHNPDCR.
OrthoDBiEOG64FKHC.
PhylomeDBiP43751.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLGRIHSF ESCGTVDGPG IRFILFMQGC LMRCKYCHNR DTWDLEGGKE
60 70 80 90 100
ISVEDLMKEV VTYRHFMNAT GGGVTASGGE AVLQAEFVRD WFRACKEEGI
110 120 130 140 150
NTCLDTNGFV RHYDHIIDEL LDVTDLVLLD LKELNDQVHQ NLIGVPNKRT
160 170 180 190 200
LEFAKYLQKR NQHTWIRYVV VPGYTDSDHD VHLLGQFIEG MTNIEKVELL
210 220 230 240
PYHRLGVHKW KTLGLDYELE NVLPPTKESL EHIKTILEGY GHTVKF
Length:246
Mass (Da):28,235
Last modified:January 23, 2007 - v2
Checksum:i884313BD5B84376F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21848.1.
PIRiE64052.
RefSeqiNP_438347.1. NC_000907.1.
WP_005694108.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21848; AAC21848; HI_0179.
GeneIDi951086.
KEGGihin:HI0179.
PATRICi20188853. VBIHaeInf48452_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21848.1.
PIRiE64052.
RefSeqiNP_438347.1. NC_000907.1.
WP_005694108.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21848; AAC21848; HI_0179.
GeneIDi951086.
KEGGihin:HI0179.
PATRICi20188853. VBIHaeInf48452_0183.

Phylogenomic databases

eggNOGiCOG1180.
KOiK04069.
OMAiCHNPDCR.
OrthoDBiEOG64FKHC.
PhylomeDBiP43751.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiPFLA_HAEIN
AccessioniPrimary (citable) accession number: P43751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.