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Protein

Pyruvate formate-lyase 1-activating enzyme

Gene

pflA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi34Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi37Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei203S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 1-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 1
PFL-activating enzyme 1
Gene namesi
Name:pflA
Synonyms:act
Ordered Locus Names:HI_0179
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002005252 – 246Pyruvate formate-lyase 1-activating enzymeAdd BLAST245

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0179.

Structurei

3D structure databases

ProteinModelPortaliP43751.
SMRiP43751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 38S-adenosyl-L-methionine bindingBy similarity3
Regioni130 – 132S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A. Bacteria.
COG1180. LUCA.
KOiK04069.
OMAiIGVPNKR.
PhylomeDBiP43751.

Family and domain databases

InterProiView protein in InterPro
IPR034457. Organic_radical-activating.
IPR012839. Organic_radical_activase.
IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
SFLDiSFLDG01066. organic_radical-activating_enz. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiView protein in PROSITE
PS01087. RADICAL_ACTIVATING. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLGRIHSF ESCGTVDGPG IRFILFMQGC LMRCKYCHNR DTWDLEGGKE
60 70 80 90 100
ISVEDLMKEV VTYRHFMNAT GGGVTASGGE AVLQAEFVRD WFRACKEEGI
110 120 130 140 150
NTCLDTNGFV RHYDHIIDEL LDVTDLVLLD LKELNDQVHQ NLIGVPNKRT
160 170 180 190 200
LEFAKYLQKR NQHTWIRYVV VPGYTDSDHD VHLLGQFIEG MTNIEKVELL
210 220 230 240
PYHRLGVHKW KTLGLDYELE NVLPPTKESL EHIKTILEGY GHTVKF
Length:246
Mass (Da):28,235
Last modified:January 23, 2007 - v2
Checksum:i884313BD5B84376F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21848.1.
PIRiE64052.
RefSeqiNP_438347.1. NC_000907.1.
WP_005694108.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21848; AAC21848; HI_0179.
GeneIDi951086.
KEGGihin:HI0179.
PATRICifig|71421.8.peg.183.

Similar proteinsi

Entry informationi

Entry nameiPFLA_HAEIN
AccessioniPrimary (citable) accession number: P43751
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families