P43748 (HOLB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit delta'
EC=2.7.7.7

Gene names

Name:	holB
Ordered Locus Names:	HI_0455

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Ontologies

Keywords
Biological process	DNA replication
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	DNA polymerase III subunit delta'		PRO_0000105514

Sequences

	Sequence		Length	Mass (Da)
	P43748 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: CEE4D452E371A298	FASTA	327	37,039
	10 20 30 40 50 60 MTALYPWLMP IYHQIAQTFD EGLGHHAVLI KADSGLGVES LFNALAQKIM CVAQGDKPCG 70 80 90 100 110 120 QCHSCHLMQA HSHPDYHELS PINGKDIGVD QVRDINEMVA QHAQQNGNKV VYVQGAERLT 130 140 150 160 170 180 EAAANALLKT LEEPRPNTYF LLQADSSASL LATIYSRCQV WNLSVPNEEI AFEWLKSKSA 190 200 210 220 230 240 VENQEILTAL AMNLGRPLLA LETLQEGFIE QRKNFLRQFW VFYRRRSPLE LLPLFDKERY 250 260 270 280 290 300 VQQVDWILAF LSDCLKHKLE IDSHRQVADL GRGIEQFSDE QTALGLLQAI KIMQKVRSDL 310 320 LTINGVNVEL MLLDGLTRLV TEVFETQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22113.1.
RefSeq	NP_438616.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P43748.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949546.
GenomeReviews	Gene locus HI_0455 in contig L42023_GR.
KEGG	hin:HI0455.
NMPDR	fig|71421.1.peg.429.
PATRIC	20189465. VBIHaeInf48452_0475.
TIGR	HI_0455.
Phylogenomic databases
HOGENOM	HBG729459.
OMA	CHSCHLM.
ProtClustDB	PRK06871.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0455-MONOMER.
Family and domain databases
InterPro	IPR008921. DNA_pol3_clamp-load_cplx_C. IPR004622. DNA_pol_HolB. IPR015199. DNA_pol_III_delta_C. [Graphical view]
KO	K02341.
Pfam	PF09115. DNApol3-delta_C. 1 hit. [Graphical view]
SUPFAM	SSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMs	TIGR00678. HolB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HOLB_HAEIN

Accession

Primary (citable) accession number: P43748

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program