Reviewed, UniProtKB/Swiss-Prot P43732 (GRPE_HAEIN)

Names and origin

Protein names

Recommended name:
    Protein grpE
Alternative name(s):
    HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	HI0071

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the grpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro protein import into mitochondrial matrix Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: InterPro
Molecular function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro chaperone binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Protein grpE HAMAP MF_01151		PRO_0000113793

Sequences

	Sequence		Length	Mass (Da)
	P43732-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: DDCA4C1B03D50EC0	FASTA	198	22,298
	10 20 30 40 50 60 MSEQEQKVEI PEVEKQEEVV VEETQQAEHS QEFDPLEEAI ARVQELEEQL KTQIEEAANK 70 80 90 100 110 120 EQDILLRSRA EIENLRRRTE QDVEKAHKFA LEKFSKDILN TIDNLERALA TPANKEDESV 130 140 150 160 170 180 KALFDGVELT LKELVSTVGR FGVEAVGVVG EAFNPDLHQA ISMQPAEGFE TNQISVVLQK 190 GYTLNGRVIR PAMVMVAA

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21750.1. Different initiation.
PIR	I64046.
RefSeq	NP_438245.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950971.
GenomeReviews	Gene locus HI0071 in contig L42023_GR.
KEGG	hin:HI0071.
NMPDR	fig|71421.1.peg.71.
TIGR	HI0071.
Phylogenomic databases
HOGENOM	HBG732630.
OMA	NLHQAVM.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0071-MON.
Family and domain databases
HAMAP	MF_01151. [Tree]
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
Gene3D	G3DSA:3.90.20.20. GrpE_coiled_coil. 1 hit. G3DSA:2.30.22.10. GrpE_head. 1 hit.
PANTHER	PTHR21237. GrpE. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_HAEIN

Accession

Primary (citable) accession number: P43732

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 30, 2000
Last modified:	December 15, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)