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P43704 (TOP3_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:HI_0444
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length651 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 651651DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000145187

Regions

Domain1 – 134134Toprim
Region194 – 1996Interaction with DNA By similarity

Sites

Active site3371O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding71Magnesium 1; catalytic By similarity
Metal binding1031Magnesium 1; catalytic By similarity
Metal binding1031Magnesium 2 By similarity
Metal binding1051Magnesium 2 By similarity
Site611Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site1781Interaction with DNA By similarity
Site1851Interaction with DNA By similarity
Site3391Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P43704 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 73D1A25F0C6C4229

FASTA65174,067
        10         20         30         40         50         60 
MRLFIAEKPS LARAIADVLP KPHQRGDGFI KCGDNDVVTW CVGHLLEQAE PDAYDPKFKQ 

        70         80         90        100        110        120 
WRLEHLPIIP EKWQLLPRKE VKKQLSVVEK LIHQADTLVN AGDPDREGQL LVDEVFSYAN 

       130        140        150        160        170        180 
LSAEKRDKIL RCLISDLNPS AVEKAVKKLQ PNRNFIPLAT SALARARADW LYGINMTRAY 

       190        200        210        220        230        240 
TIRGRQTGYD GVLSVGRVQT PVLGLIVRRD LEIEHFQPKD FFEVQAWVNP ESKEEKTPEK 

       250        260        270        280        290        300 
STALFSALWQ PSKACEDYQD DDGRVLSKGL AEKVVKRITN QPAEVTEYKD VREKETAPLP 

       310        320        330        340        350        360 
YSLSALQIDA AKRFGMSAQA VLDTCQRLYE THRLITYPRS DCRYLPEEHF AERHNVLNAI 

       370        380        390        400        410        420 
STHCEAYQVL PNVILTEQRN RCWNDKKVEA HHAIIPTAKN RPVNLTQEER NIYSLIARQY 

       430        440        450        460        470        480 
LMQFCPDAEY RKSKITLNIA GGTFIAQARN LQTAGWKELL GKEDDTENQE PLLPIVKKGQ 

       490        500        510        520        530        540 
ILHCERGEVM SKKTQPPKPF TDATLLSAMT GIARFVQDKE LKKILRETDG LGTEATRAGI 

       550        560        570        580        590        600 
IELLFKRGFL TKKGRNIHST ETGRILIQAL PNIATQPDMT AHWESQLTDI SQKQATYQQF 

       610        620        630        640        650 
MHNLNQILPD LVRFVDLNAL RQLSRIKMIK SDRAKPKSAV KKSSKSNGET D 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22103.1.
PIRG64068.
RefSeqNP_438605.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43704.
SMRP43704. Positions 1-612.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0444.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22103; AAC22103; HI_0444.
GeneID949541.
KEGGhin:HI0444.
PATRIC20189441. VBIHaeInf48452_0464.

Phylogenomic databases

eggNOGCOG0550.
KOK03169.
OMAFCPDAEY.
OrthoDBEOG60CWM6.
ProtClustDBPRK07726.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_HAEIN
AccessionPrimary (citable) accession number: P43704
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names