Reviewed, UniProtKB/Swiss-Prot P43702 (PARC_HAEIN)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 4 subunit A
    EC=5.99.1.-
Alternative name(s):
    Topoisomerase IV subunit A

Gene names

Name:	parC
Ordered Locus Names:	HI1529

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It has relaxation of supercoiled DNA activity. Performs the decatenation events required during the replication of a circular DNA molecule By similarity.
Subunit structure	Composed of two subunits: parC and parE.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	DNA-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	DNA topoisomerase 4 subunit A		PRO_0000145400
Sites
	Active site	124	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43702-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 433038EA479C8D01	FASTA	747	83,367
	10 20 30 40 50 60 MTNINYEGIE QMPLRTFTEK AYLNYSMYVI MDRALPFIGD GLKPVQRRIV YAMSELGLNA 70 80 90 100 110 120 TAKYKKSART VGDVLGKFHP HGDSACYEAM VLMAQPFSYR YPLVDGQGNW GAPDDPKSFA 130 140 150 160 170 180 AMRYTESRLS KISEILLNEL GQGTVDYQPN FDGTLAEPQY LPARLPHILL NGTTGIAVGM 190 200 210 220 230 240 ATDIPPHNIN EIADAAVMLL DNPKAGLDDV LEIVQGPDFP TEAEIISPKS EIRKIYEQGR 250 260 270 280 290 300 GSIKMRATWK KEDGEIIISA LPHQSSPSKV IAQIAEQMTA KKLPMLEDIR DEADHENPIR 310 320 330 340 350 360 IVLVPRSNRV DTDALMAHLF ATTDLEKSYR VNMNMIGLDH KPAVKGLLEI LNEWLDFRRT 370 380 390 400 410 420 TVTRRLQYRL DKVLSRLHIL EGLMIAFLNI DEVIEIIRHE DDPKAELMAR FNLSDEQADA 430 440 450 460 470 480 ILNLRLRHLA KLEENQLKAE QDELEKERLN LEAILGSERR LNTLIKKEIQ EDAKKYANPR 490 500 510 520 530 540 MSQLVEREEA KMISESDMTP AEPVTVILSE MGWVRCAKGH DIDPKSLSYK AGDSYLAHAC 550 560 570 580 590 600 GKSNQAVVFI DSTGRSYALD PLSLPSARSQ GEPLTGKLNL PTGATIEYVV MASEQQELLM 610 620 630 640 650 660 ASDAGYGFIC KFEDLIARNK AGKALISLPE NAKVLKPKTL INSTALVVAI TSAGRMLIFP 670 680 690 700 710 720 AQDLPVLSKG KGNKMITIPA ANAKDRSELL TKLLLISDQA SLEFYSGKRK IVLKPEDLQK 730 740 FRAERGRKGS TLPRGLHTNL EIMVIEP

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC23175.1.
PIR	F64127.
RefSeq	NP_439678.1.
3D structure databases
SMR	P43702. Positions 32-737.
ModBase	Search...
Genome annotation databases
GeneID	950391.
GenomeReviews	Gene locus HI1529 in contig L42023_GR.
KEGG	hin:HI1529.
NMPDR	fig|71421.1.peg.1456.
TIGR	HI1529.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	ILAITNE.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1529-MONOMER.
Family and domain databases
InterPro	IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. IPR005742. TopoIV_A_Gneg. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 2 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01062. parC_Gneg. 1 hit.
ProtoNet	Search...
Other Resources
DrugBank	DB00537. Ciprofloxacin. DB00467. Enoxacin. DB01044. Gatifloxacin. DB01155. Gemifloxacin. DB00365. Grepafloxacin. DB01137. Levofloxacin. DB00978. Lomefloxacin. DB00218. Moxifloxacin. DB01059. Norfloxacin. DB01165. Ofloxacin. DB00487. Pefloxacin. DB01208. Sparfloxacin. DB01405. Temafloxacin. DB00685. Trovafloxacin.

Entry information

Entry name

PARC_HAEIN

Accession

Primary (citable) accession number: P43702

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)