Reviewed, UniProtKB/Swiss-Prot P43700 (GYRA_HAEIN)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	HI1264

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	DNA gyrase subunit A		PRO_0000145235
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43700-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0B2E9DD34155A322	FASTA	880	97,818
	10 20 30 40 50 60 MTDSIQSSIT PVNIEEELKS SYLDYAMSVI VGRALPDVRD GLKPVHRRVL FSMDREGNTA 70 80 90 100 110 120 NKKYVKSARV VGDVIGKYHP HGDSAVYDTI VRMAQPFSLR YMLVDGQGNF GSIDGDAPAA 130 140 150 160 170 180 MRYTEVRMQK ITQALLTDLD KETVNFSPNY DGELMIPDVL PTRIPALLAN GSSGIAVGMA 190 200 210 220 230 240 TNIPPHNLNE VLNGCLAYID KNEITIDELM QHIPGPDFPT AALINGRKGI EEAYRTGRGK 250 260 270 280 290 300 VYVRARATVE TNEKGREQII VSELPYQVNK AKLVEKIAEL IREKKIEGIS NITDLSNKEG 310 320 330 340 350 360 IRIEIDIKRD AVGEVVLNHL YSLTQMQVTF GINMVALDHG QPRLFNLKEI IEAFVLHRRE 370 380 390 400 410 420 VVTRRSIFEL RKARERTHIL EGLAVARSNI DEMIAIIRNS KNREEAATSI SSRSWTLHSD 430 440 450 460 470 480 IINLLDASAR PDELEENLGI QGEQYYLSPA QVNAILELRL HRLTGIAFEE VIKEYEELLV 490 500 510 520 530 540 KIADLLHILS SAERLMEVIR EELEEVKAQF GDDRLTEITA ASGDIDLEDL IAQEDVVVTL 550 560 570 580 590 600 SHEGYVKYQP LTDYEAQRRG GKGKSATKMK EEDFIEKLLV ANTHDTILCF SSRGRLYWLK 610 620 630 640 650 660 VYQLPQASRG ARGRPIVNIL PLQENERITA ILPVSAYEED KFVVMATAGG IVKKIALTEF 670 680 690 700 710 720 SRPRSNGIIA LNLRDEDELI GVDITDGSNE IMLFSSQGRV VRFAENAVRA MGRLATGVRG 730 740 750 760 770 780 IKLALTNDIS DDESAVEIED ISDDNAEASL DLNIDKVVSL VVPKGEGAIL TATQNGYGKR 790 800 810 820 830 840 TQLSEYPTKS RNTKGVISIK VSERNGKVVA ATQVEETDQI MLITDAGTLV RTRVSEVSIV 850 860 870 880 GRNTQGVRLI RTADDEHVVS LERVCDADED DSLEESSSEE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC22917.1.
PIR	E64113.
RefSeq	NP_439419.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
SMR	P43700. Positions 533-866.
ModBase	Search...
Genome annotation databases
GeneID	950199.
GenomeReviews	Gene locus HI1264 in contig L42023_GR.
KEGG	hin:HI1264.
NMPDR	fig|71421.1.peg.1209.
TIGR	HI1264.
Phylogenomic databases
HOGENOM	P43700.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1264-MON.
BRENDA	5.99.1.3. 109.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...
Other Resources
DrugBank	DB00537. Ciprofloxacin. DB00467. Enoxacin. DB01044. Gatifloxacin. DB01155. Gemifloxacin. DB00365. Grepafloxacin. DB01137. Levofloxacin. DB00978. Lomefloxacin. DB00218. Moxifloxacin. DB01059. Norfloxacin. DB01165. Ofloxacin. DB00487. Pefloxacin. DB01208. Sparfloxacin. DB01405. Temafloxacin. DB00685. Trovafloxacin.

Entry information

Entry name

GYRA_HAEIN

Accession

Primary (citable) accession number: P43700

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)