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Protein

Homeobox protein Nkx-2.6

Gene

Nkx2-6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a transcriptional activator (By similarity). In conjunction with NKX2-5, may play a role in both pharyngeal and cardiac embryonic development (PubMed:10733590, PubMed:11390666).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi123 – 18260HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • atrial cardiac muscle cell development Source: BHF-UCL
  • cell differentiation Source: MGI
  • digestive tract development Source: BHF-UCL
  • embryonic heart tube development Source: BHF-UCL
  • heart development Source: MGI
  • hypothalamus development Source: BHF-UCL
  • negative regulation of apoptotic process Source: MGI
  • pericardium development Source: BHF-UCL
  • pharyngeal system development Source: BHF-UCL
  • positive regulation of cell proliferation Source: MGI
  • tongue development Source: BHF-UCL
  • transcription, DNA-templated Source: MGI
  • ventricular cardiac muscle cell development Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-2.6
Alternative name(s):
Homeobox protein NK-2 homolog F
Gene namesi
Name:Nkx2-6
Synonyms:Nkx-2.6, Nkx2f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97351. Nkx2-6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; possibly due to the redundancy with NKX2-6. Mutant mice are viable and fertile. No obvious abnormalities in the caudal pharyngeal pouch derivatives (thymus, parathyroid glands, and thyroid gland), heart and gut. In NKX2-5 and NKX2-6 double mutants, the pharynx does not form properly and the development of the atrium is less advanced.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Homeobox protein Nkx-2.6PRO_0000048942Add
BLAST

Proteomic databases

PaxDbiP43688.
PRIDEiP43688.

PTM databases

iPTMnetiP43688.
PhosphoSiteiP43688.

Expressioni

Tissue specificityi

Not detected in any neonate or adult tissues.1 Publication

Developmental stagei

Expressed in developing gut endoderm, cardiac progenitors and heart. Expression restricted to the very narrow development period between stages 8.0 and 11.5 dpc. First detected at 8.0 dpc in the endoderm of the lateral walls and lip of the forming foregut pocket, directly juxtaposed to cardiogenic mesoderm. At 8.5 dpc, expressed in the forming pharynx, predominantly in its lateral aspects where pouches will form. From 8.5 to 11.5 dpc, expressed in the ventrolateral endoderm of all forming pouches, as well as in juxtaposed arch ectoderm and mesenchyme. Expression levels decrease as pouches matured. Also expressed in the ventral hindgut endoderm from 9.5 dpc and in a short segment at the foregut-midgut junction spanning the proximal parts of the common bile and pancreatic ducts.2 Publications

Gene expression databases

BgeeiP43688.
GenevisibleiP43688. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049898.

Structurei

3D structure databases

ProteinModelPortaliP43688.
SMRiP43688. Positions 123-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NK-2 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
GeneTreeiENSGT00840000129671.
HOGENOMiHOG000231923.
HOVERGENiHBG006689.
InParanoidiP43688.
KOiK09346.
OMAiGRTRDSE.
OrthoDBiEOG769ZK0.
PhylomeDBiP43688.
TreeFamiTF351204.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSPVASTP FSVADILRLE CQQKDSKTLS QWELHRNPVK PRYLRMNQES
60 70 80 90 100
GWFESSDRAQ AVPFRCTWET VLEMGSNPVG EPQTPPGTIS RLGARNPMTD
110 120 130 140 150
RGVGNLSGDM RRGGPVSTRT RPQRKSRVLF SQAQVLALER RFKQQRYLTA
160 170 180 190 200
PEREHLASAL QLTSTQVKIW FQNRRYKSKS QRQDQTLELA GHPLAPRRVA
210 220 230 240 250
VPVLVLDGKP CLDPDVAAFL GPYKATSPYS CFGGYAGTPY DASYASRCTS
260 270 280
ASAGPGPLTP LASSGFSPGG QSAAPQGHLP ATPQGVTAW
Length:289
Mass (Da):31,597
Last modified:December 15, 1998 - v2
Checksum:iDBF8740A39CC0805
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045150 mRNA. Translation: AAC15674.1.
CCDSiCCDS56968.1.
RefSeqiNP_035050.2. NM_010920.2.
UniGeneiMm.390691.

Genome annotation databases

EnsembliENSMUST00000062437; ENSMUSP00000049898; ENSMUSG00000044186.
GeneIDi18092.
KEGGimmu:18092.
UCSCiuc007umb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045150 mRNA. Translation: AAC15674.1.
CCDSiCCDS56968.1.
RefSeqiNP_035050.2. NM_010920.2.
UniGeneiMm.390691.

3D structure databases

ProteinModelPortaliP43688.
SMRiP43688. Positions 123-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049898.

PTM databases

iPTMnetiP43688.
PhosphoSiteiP43688.

Proteomic databases

PaxDbiP43688.
PRIDEiP43688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062437; ENSMUSP00000049898; ENSMUSG00000044186.
GeneIDi18092.
KEGGimmu:18092.
UCSCiuc007umb.2. mouse.

Organism-specific databases

CTDi137814.
MGIiMGI:97351. Nkx2-6.

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
GeneTreeiENSGT00840000129671.
HOGENOMiHOG000231923.
HOVERGENiHBG006689.
InParanoidiP43688.
KOiK09346.
OMAiGRTRDSE.
OrthoDBiEOG769ZK0.
PhylomeDBiP43688.
TreeFamiTF351204.

Miscellaneous databases

PROiP43688.
SOURCEiSearch...

Gene expression databases

BgeeiP43688.
GenevisibleiP43688. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of NK-2 class homeobox gene Nkx2-6 in foregut endoderm and heart."
    Biben C., Hatzistavrou T., Wang C.-C., Harvey R.P.
    Mech. Dev. 73:125-127(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: BALB/cJ.
  2. "Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants."
    Lints T.J., Parsons L.M., Hartley L., Lyons I., Harvey R.P.
    Development 119:419-431(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 123-214.
    Tissue: Heart.
  3. "Nkx2.6 expression is transiently and specifically restricted to the branchial region of pharyngeal-stage mouse embryos."
    Nikolova M., Chen X., Lufkin T.
    Mech. Dev. 69:215-218(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. "Phenotypic characterization of the murine Nkx2.6 homeobox gene by gene targeting."
    Tanaka M., Yamasaki N., Izumo S.
    Mol. Cell. Biol. 20:2874-2879(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. "Nkx2.5 and Nkx2.6, homologs of Drosophila tinman, are required for development of the pharynx."
    Tanaka M., Schinke M., Liao H.S., Yamasaki N., Izumo S.
    Mol. Cell. Biol. 21:4391-4398(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiNKX26_MOUSE
AccessioniPrimary (citable) accession number: P43688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: June 8, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.