P43677 (ACPX_STRRM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Oxytetracycline polyketide synthase acyl carrier protein Short name=ACP
Organism	Streptomyces rimosus
Taxonomic identifier	1927 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acyl carrier protein.
Pathway	Antibiotic biosynthesis; oxytetracycline biosynthesis.
Post-translational modification	4'-phosphopantetheine is transferred from CoA to a specific serine of the apo-ACP-like protein Potential.
Sequence similarities	Contains 1 acyl carrier domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Phosphopantetheine
Technical term	3D-structure
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 95

95

Oxytetracycline polyketide synthase acyl carrier protein

PRO_0000180253

Amino acid modifications

Modified residue

41

1

O-(pantetheine 4'-phosphoryl)serine Probable

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

95

Helix Strand Turn

Details...

Helix

6 – 17

12

Beta strand

30 – 32

3

Helix

34 – 37

4

Helix

42 – 54

13

Helix

63 – 66

4

Helix

70 – 83

14

Sequences

	Sequence		Length	Mass (Da)
	P43677 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D0C92084807BB244	FASTA	95	9,916
	10 20 30 40 50 60 MTLLTLSDLL TLLRECAGEE ESIDLGGDVE DVAFDALGYD SLALLNTVGR IERDYGVQLG 70 80 90 DDAVEKATTP RALIEMTNAS LTGASPSAGG AARDK

References

[1]	"Sequences of the oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus." Kim E.S., Bibb M.J., Butler M.J., Hopwood D.A., Sherman D.H. Gene 141:141-142(1994) [PubMed: 8163168] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The acyl-carrier protein for biosynthesis of oxytetracycline from Streptomyces rimosus: overexpression in Escherichia coli, purification and characterization of the apoprotein." Linton K.J., Hunter I.S. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 15883.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z25538 Genomic DNA. Translation: CAA80986.1.
U01666 Genomic DNA. Translation: AAA03334.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NQ4	NMR	-	A	1-95	[»]

ProteinModelPortal

P43677.

SMR

P43677. Positions 14-95.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009081. Acyl_carrier_prot-like.
IPR006163. Phsphopanteth-bd.
IPR006162. PPantetheine_attach_site.
[Graphical view]

Gene3D

G3DSA:1.10.1200.10. ACP_like. 1 hit.

Pfam

PF00550. PP-binding. 1 hit.
[Graphical view]

SUPFAM

SSF47336. ACP_like. 1 hit.

PROSITE

PS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ACPX_STRRM

Accession

Primary (citable) accession number: P43677

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 30, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program