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Protein

Probable low-affinity inorganic phosphate transporter 2

Gene

pitB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Low-affinity inorganic phosphate transport.By similarity

GO - Molecular functioni

  • inorganic phosphate transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • phosphate ion transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PITB-MONOMER.
ECOL316407:JW2955-MONOMER.
MetaCyc:PITB-MONOMER.

Protein family/group databases

TCDBi2.A.20.1.2. the inorganic phosphate transporter (pit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable low-affinity inorganic phosphate transporter 2
Gene namesi
Name:pitB
Ordered Locus Names:b2987, JW2955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12883. pitB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521HelicalSequence analysisAdd
BLAST
Transmembranei52 – 7221HelicalSequence analysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence analysisAdd
BLAST
Transmembranei155 – 17521HelicalSequence analysisAdd
BLAST
Transmembranei207 – 22721HelicalSequence analysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence analysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence analysisAdd
BLAST
Transmembranei430 – 45021HelicalSequence analysisAdd
BLAST
Transmembranei473 – 49321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Probable low-affinity inorganic phosphate transporter 2PRO_0000080785Add
BLAST

Proteomic databases

PaxDbiP43676.

Interactioni

Protein-protein interaction databases

BioGridi4261181. 18 interactions.
STRINGi511145.b2987.

Structurei

3D structure databases

ProteinModelPortaliP43676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJ3. Bacteria.
COG0306. LUCA.
HOGENOMiHOG000231893.
InParanoidiP43676.
KOiK16322.
OMAiLALTFDF.
OrthoDBiEOG654P07.
PhylomeDBiP43676.

Family and domain databases

InterProiIPR001204. Phos_transporter.
[Graphical view]
PANTHERiPTHR11101. PTHR11101. 1 hit.
PfamiPF01384. PHO4. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNLFVGLDI YTGLLLLLAL AFVLFYEAIN GFHDTANAVA AVIYTRAMQP
60 70 80 90 100
QLAVVMAAFF NFFGVLLGGL SVAYAIVHML PTDLLLNMGS THGLAMVFSM
110 120 130 140 150
LLAAIIWNLG TWFFGLPASS SHTLIGAIIG IGLTNALLTG SSVMDALNLR
160 170 180 190 200
EVTKIFSSLI VSPIVGLVIA GGLIFLLRRY WSGTKKRDRI HRIPEDRKKK
210 220 230 240 250
KGKRKPPFWT RIALIVSAAG VAFSHGANDG QKGIGLVMLV LVGIAPAGFV
260 270 280 290 300
VNMNASGYEI TRTRDAVTNF EHYLQQHPEL PQKLIAMEPP LPAASTDGTQ
310 320 330 340 350
VTEFHCHPAN TFDAIARVKT MLPGNMESYE PLSVSQRSQL RRIMLCISDT
360 370 380 390 400
SAKLAKLPGV SKEDQNLLKK LRSDMLSTIE YAPVWIIMAV ALALGIGTMI
410 420 430 440 450
GWRRVAMTIG EKIGKRGMTY AQGMAAQMTA AVSIGLASYI GMPVSTTHVL
460 470 480 490
SSAVAGTMVV DGGGLQRKTV TSILMAWVFT LPAAIFLSGG LYWIALQLI
Length:499
Mass (Da):53,809
Last modified:November 1, 1995 - v1
Checksum:i1D3677057FAB295B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821S → C in AAC43340 (PubMed:7775463).Curated
Sequence conflicti213 – 2131A → G in AAC43340 (PubMed:7775463).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69154.1.
U00096 Genomic DNA. Translation: AAC76023.1.
AP009048 Genomic DNA. Translation: BAE77048.1.
U23148 Genomic DNA. Translation: AAC43340.1.
PIRiA65085.
RefSeqiNP_417461.1. NC_000913.3.
WP_000933250.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76023; AAC76023; b2987.
BAE77048; BAE77048; BAE77048.
GeneIDi947475.
KEGGiecj:JW2955.
eco:b2987.
PATRICi32121390. VBIEscCol129921_3082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69154.1.
U00096 Genomic DNA. Translation: AAC76023.1.
AP009048 Genomic DNA. Translation: BAE77048.1.
U23148 Genomic DNA. Translation: AAC43340.1.
PIRiA65085.
RefSeqiNP_417461.1. NC_000913.3.
WP_000933250.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP43676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261181. 18 interactions.
STRINGi511145.b2987.

Protein family/group databases

TCDBi2.A.20.1.2. the inorganic phosphate transporter (pit) family.

Proteomic databases

PaxDbiP43676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76023; AAC76023; b2987.
BAE77048; BAE77048; BAE77048.
GeneIDi947475.
KEGGiecj:JW2955.
eco:b2987.
PATRICi32121390. VBIEscCol129921_3082.

Organism-specific databases

EchoBASEiEB2721.
EcoGeneiEG12883. pitB.

Phylogenomic databases

eggNOGiENOG4105CJ3. Bacteria.
COG0306. LUCA.
HOGENOMiHOG000231893.
InParanoidiP43676.
KOiK16322.
OMAiLALTFDF.
OrthoDBiEOG654P07.
PhylomeDBiP43676.

Enzyme and pathway databases

BioCyciEcoCyc:PITB-MONOMER.
ECOL316407:JW2955-MONOMER.
MetaCyc:PITB-MONOMER.

Miscellaneous databases

PROiP43676.

Family and domain databases

InterProiIPR001204. Phos_transporter.
[Graphical view]
PANTHERiPTHR11101. PTHR11101. 1 hit.
PfamiPF01384. PHO4. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase."
    Bollinger J.M. Jr., Kwon D.S., Huisman G.W., Kolter R., Walsh C.T.
    J. Biol. Chem. 270:14031-14041(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-217.
    Strain: K12.
  4. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPITB_ECOLI
AccessioniPrimary (citable) accession number: P43676
Secondary accession number(s): Q2M9K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 16, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.