P43674 (YCAL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized metalloprotease YcaL
EC=3.4.24.-

Gene names

Name:	ycaL
Ordered Locus Names:	b0909, JW0892

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit Potential.
Sequence similarities	Belongs to the peptidase M48B family.
Sequence caution	The sequence AAA81515.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Uncharacterized metalloprotease YcaL		PRO_0000138937
Sites
	Active site	135	1	Potential
	Metal binding	134	1	Zinc; catalytic Potential
	Metal binding	138	1	Zinc; catalytic Potential
	Metal binding	193	1	Zinc; catalytic By similarity
Experimental info
	Sequence conflict	241 – 254	14	ERAQH…IASGK → DVRNTSVIVSPLVSKSLSSL RWSSASPL in CAA58106. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P43674 [UniParc]. Last modified July 15, 1998. Version 3. Checksum: 4DDF70D17E9D93A0	FASTA	254	26,740
	10 20 30 40 50 60 MKNTKLLLAI ATSAALLTGC QNTHGIDTNM AISSGLNAYK AATLSDADAK AIANQGCAEM 70 80 90 100 110 120 DSGNQVASKS SKYGKRLAKI AKALGNNING TPVNYKVYMT SDVNAWAMAN GCVRVYSGLM 130 140 150 160 170 180 DMMNDNEIEG VLGHELGHVA LGHSLAEMKA SYAIVAARDA ISATSGVASQ LSRSQLGDIA 190 200 210 220 230 240 EGAINAKYSR DKESEADDFS FDLLKKRGIS TQGLVGSFET LASLDGGRTQ SMFDSHPPST 250 ERAQHIRDRI ASGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Palma C.A., Allen E., Araujo R., Aparicio A.M., Botstein D., Cherry M., Chung E., Dietrich F., Duncan M., Federspiel N., Kalman S., Kim K., Komp C., Lashkari D., Lew H., Lin D., Namath A., Oefner P., Davis R. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli." Fricke J., Neuhard J., Kelln R.A., Pedersen S. J. Bacteriol. 177:517-523(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 150-254. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73995.2. AP009048 Genomic DNA. Translation: BAA35644.1. U31523 Genomic DNA. Translation: AAA81515.1. Different initiation. X82933 Genomic DNA. Translation: CAA58106.1.
PIR	D64830.
RefSeq	NP_415429.2. NC_000913.2. YP_489181.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P43674.
SMR	P43674. Positions 44-251.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11467N.
STRING	511145.b0909.
Protein family/group databases
MEROPS	M48.A03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73995; AAC73995; b0909. BAA35644; BAA35644; BAA35644.
GeneID	12931018. 945534.
KEGG	ecj:Y75_p0881. eco:b0909.
PATRIC	32117031. VBIEscCol129921_0940.
Organism-specific databases
EchoBASE	EB2932.
EcoGene	EG13133. ycaL.
Phylogenomic databases
eggNOG	COG0501.
HOGENOM	HOG000264551.
KO	K07387.
OMA	INAFACA.
ProtClustDB	CLSK865738.
Enzyme and pathway databases
BioCyc	EcoCyc:G6470-MONOMER. ECOL316407:JW0892-MONOMER.
Gene expression databases
Genevestigator	P43674.
Family and domain databases
InterPro	IPR001915. Peptidase_M48. [Graphical view]
Pfam	PF01435. Peptidase_M48. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YCAL_ECOLI

Accession

Primary (citable) accession number: P43674
Secondary accession number(s): P75840

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program