Reviewed,
UniProtKB/Swiss-Prot P43652 (AFAM_HUMAN)
Last modified
December 15, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Afamin Alternative name(s): Alpha-albumin Short name=Alpha-Alb | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 599 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Possible role in the transport of yet unknown ligand. |
| Subcellular location | |
| Post-translational modification | |
| Sequence similarities | Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transport |
| Cellular component | Secreted |
| Coding sequence diversity | Polymorphism |
| Domain | Repeat Signal |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | transport Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular space Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 21 | 21 | Potential | ||||||||
| Chain | 22 – 599 | 578 | Afamin | PRO_0000001106 | |||||||
Regions | |||||||||||
| Domain | 22 – 210 | 189 | Albumin 1 | ||||||||
| Domain | 211 – 403 | 193 | Albumin 2 | ||||||||
| Domain | 404 – 599 | 196 | Albumin 3 | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 33 | 1 | N-linked (GlcNAc...) Ref.8 Ref.9 Ref.10 | ||||||||
| Glycosylation | 109 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 402 | 1 | N-linked (GlcNAc...) Ref.7 Ref.8 Ref.10 | ||||||||
| Glycosylation | 488 | 1 | N-linked (GlcNAc...) Ref.8 | ||||||||
| Disulfide bond | 77 ↔ 86 | By similarity | |||||||||
| Disulfide bond | 99 ↔ 114 | By similarity | |||||||||
| Disulfide bond | 113 ↔ 124 | By similarity | |||||||||
| Disulfide bond | 148 ↔ 193 | By similarity | |||||||||
| Disulfide bond | 192 ↔ 201 | By similarity | |||||||||
| Disulfide bond | 224 ↔ 270 | By similarity | |||||||||
| Disulfide bond | 269 ↔ 277 | By similarity | |||||||||
| Disulfide bond | 289 ↔ 303 | By similarity | |||||||||
| Disulfide bond | 302 ↔ 313 | By similarity | |||||||||
| Disulfide bond | 340 ↔ 385 | By similarity | |||||||||
| Disulfide bond | 384 ↔ 393 | By similarity | |||||||||
| Disulfide bond | 416 ↔ 462 | By similarity | |||||||||
| Disulfide bond | 461 ↔ 470 | By similarity | |||||||||
| Disulfide bond | 483 ↔ 499 | By similarity | |||||||||
| Disulfide bond | 498 ↔ 509 | By similarity | |||||||||
| Disulfide bond | 536 ↔ 581 | By similarity | |||||||||
| Disulfide bond | 580 ↔ 589 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 395 | 1 | R → H: dbSNP rs41265665. | VAR_061003 | |||||||
| Natural variant | 404 | 1 | T → S: dbSNP rs2276444. | VAR_048218 | |||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Afamin is a new member of the albumin, alpha-fetoprotein, and vitamin D-binding protein gene family." Lichenstein H.S., Lyons D.E., Wurfel M.M., Johnson D.A., McGinley M.D., Leidli J.C., Trollinger D.B., Mayer J.P., Wright S.D., Zukowski M.M. J. Biol. Chem. 269:18149-18154(1994) [PubMed: 7517938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver. |
| [2] | "Complete structure of the human alpha-albumin gene, a new member of the serum albumin multigene family." Nishio H., Dugaiczyk A. Proc. Natl. Acad. Sci. U.S.A. 93:7557-7561(1996) [PubMed: 8755513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [6] | "Identification of rat alpha-albumin and cDNA cloning of its human ortholog." Allard D., Gilbert S., Lamontagne A., Hamel D., Belanger L. Gene 153:287-288(1995) [PubMed: 7875606] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-69; 105-207 AND 560-599. Tissue: Liver. |
| [7] | "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry." Bunkenborg J., Pilch B.J., Podtelejnikov A.V., Wisniewski J.R. Proteomics 4:454-465(2004) [PubMed: 14760718] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-402, MASS SPECTROMETRY. Tissue: Plasma. |
| [8] | "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-402 AND ASN-488, MASS SPECTROMETRY. Tissue: Plasma. |
| [9] | "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33, MASS SPECTROMETRY. Tissue: Saliva. |
| [10] | "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-402, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L32140 mRNA. Translation: AAA21612.1. U51243 Genomic DNA. Translation: AAC50720.1. AK290556 mRNA. Translation: BAF83245.1. CH471057 Genomic DNA. Translation: EAX05682.1. BC109020 mRNA. Translation: AAI09021.1. BC109021 mRNA. Translation: AAI09022.1. L35486 mRNA. Translation: AAA68197.1. L35497 mRNA. Translation: AAA68198.1. L35498 mRNA. Translation: AAA68199.1. | |
| IPI | IPI00019943. |
| PIR | A54906. I39424. I39426. |
| RefSeq | NP_001124.1. |
| UniGene | Hs.168718 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P43652. |
PTM databases | |
| PhosphoSite | P43652. |
Proteomic databases | |
| PeptideAtlas | P43652. |
| PRIDE | P43652. |
Genome annotation databases | |
| Ensembl | ENST00000226355; ENSP00000226355; ENSG00000079557; Homo sapiens. [Genome view] |
| GeneID | 173. |
| KEGG | hsa:173. |
| UCSC | uc003hhb.1. human. |
Organism-specific databases | |
| CTD | 173. |
| GeneCards | GC04P074587. |
| H-InvDB | HIX0031507. |
| HGNC | HGNC:316. AFM. |
| HPA | HPA017006. |
| MIM | 104145. gene. |
| PharmGKB | PA24613. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG283181. |
| HOVERGEN | P43652. |
| InParanoid | P43652. |
| OMA | EFACVDN. |
| OrthoDB | EOG9962B0. |
Gene expression databases | |
| ArrayExpress | P43652. |
| Bgee | P43652. |
| CleanEx | HS_AFM. |
| Genevestigator | P43652. |
| GermOnline | ENSG00000079557. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001703. Alpha-fetoprotein. IPR000264. Serum_albumin. IPR020858. Serum_albumin-like. IPR020857. Serum_albumin_CS. IPR014760. Serum_albumin_N. [Graphical view] |
| Pfam | PF00273. Serum_albumin. 3 hits. [Graphical view] |
| PRINTS | PR00803. AFETOPROTEIN. PR00802. SERUMALBUMIN. |
| SMART | SM00103. ALBUMIN. 3 hits. [Graphical view] |
| PROSITE | PS00212. ALBUMIN_1. 3 hits. PS51438. ALBUMIN_2. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 694. |
| SOURCE | Search... |
Entry information
| Entry name | AFAM_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P43652 Secondary accession number(s): A8K3E1, Q32MR3 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


