Reviewed, UniProtKB/Swiss-Prot P43652 (AFAM_HUMAN)

Names and origin

Protein names

Recommended name:
    Afamin
Alternative name(s):
    Alpha-albumin
      Short name=Alpha-Alb

Gene names

Name:	AFM
Synonyms:	ALB2, ALBA

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible role in the transport of yet unknown ligand.
Subcellular location	Secreted.
Post-translational modification	N-glycosylated. Ref.7 Ref.8 Ref.9 Ref.10
Sequence similarities	Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 599	578	Afamin		PRO_0000001106
Regions
	Domain	22 – 210	189	Albumin 1
	Domain	211 – 403	193	Albumin 2
	Domain	404 – 599	196	Albumin 3
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Ref.8 Ref.9 Ref.10
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	402	1	N-linked (GlcNAc...) Ref.7 Ref.8 Ref.10
	Glycosylation	488	1	N-linked (GlcNAc...) Ref.8
	Disulfide bond	77 ↔ 86		By similarity
	Disulfide bond	99 ↔ 114		By similarity
	Disulfide bond	113 ↔ 124		By similarity
	Disulfide bond	148 ↔ 193		By similarity
	Disulfide bond	192 ↔ 201		By similarity
	Disulfide bond	224 ↔ 270		By similarity
	Disulfide bond	269 ↔ 277		By similarity
	Disulfide bond	289 ↔ 303		By similarity
	Disulfide bond	302 ↔ 313		By similarity
	Disulfide bond	340 ↔ 385		By similarity
	Disulfide bond	384 ↔ 393		By similarity
	Disulfide bond	416 ↔ 462		By similarity
	Disulfide bond	461 ↔ 470		By similarity
	Disulfide bond	483 ↔ 499		By similarity
	Disulfide bond	498 ↔ 509		By similarity
	Disulfide bond	536 ↔ 581		By similarity
	Disulfide bond	580 ↔ 589		By similarity
Natural variations
	Natural variant	395	1	R → H: dbSNP rs41265665.		VAR_061003
	Natural variant	404	1	T → S: dbSNP rs2276444.		VAR_048218

Sequences

	Sequence		Length	Mass (Da)
	P43652-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D594E75E20D308AB	FASTA	599	69,069
	10 20 30 40 50 60 MKLLKLTGFI FFLFFLTESL TLPTQPRDIE NFNSTQKFIE DNIEYITIIA FAQYVQEATF 70 80 90 100 110 120 EEMEKLVKDM VEYKDRCMAD KTLPECSKLP NNVLQEKICA MEGLPQKHNF SHCCSKVDAQ 130 140 150 160 170 180 RRLCFFYNKK SDVGFLPPFP TLDPEEKCQA YESNRESLLN HFLYEVARRN PFVFAPTLLT 190 200 210 220 230 240 VAVHFEEVAK SCCEEQNKVN CLQTRAIPVT QYLKAFSSYQ KHVCGALLKF GTKVVHFIYI 250 260 270 280 290 300 AILSQKFPKI EFKELISLVE DVSSNYDGCC EGDVVQCIRD TSKVMNHICS KQDSISSKIK 310 320 330 340 350 360 ECCEKKIPER GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF FAKFTFEYSR 370 380 390 400 410 420 RHPDLSIPEL LRIVQIYKDL LRNCCNTENP PGCYRYAEDK FNETTEKSLK MVQQECKHFQ 430 440 450 460 470 480 NLGKDGLKYH YLIRLTKIAP QLSTEELVSL GEKMVTAFTT CCTLSEEFAC VDNLADLVFG 490 500 510 520 530 540 ELCGVNENRT INPAVDHCCK TNFAFRRPCF ESLKADKTYV PPPFSQDLFT FHADMCQSQN 550 560 570 580 590 EELQRKTDRF LVNLVKLKHE LTDEELQSLF TNFANVVDKC CKAESPEVCF NEESPKIGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Afamin is a new member of the albumin, alpha-fetoprotein, and vitamin D-binding protein gene family." Lichenstein H.S., Lyons D.E., Wurfel M.M., Johnson D.A., McGinley M.D., Leidli J.C., Trollinger D.B., Mayer J.P., Wright S.D., Zukowski M.M. J. Biol. Chem. 269:18149-18154(1994) [PubMed: 7517938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Complete structure of the human alpha-albumin gene, a new member of the serum albumin multigene family." Nishio H., Dugaiczyk A. Proc. Natl. Acad. Sci. U.S.A. 93:7557-7561(1996) [PubMed: 8755513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Identification of rat alpha-albumin and cDNA cloning of its human ortholog." Allard D., Gilbert S., Lamontagne A., Hamel D., Belanger L. Gene 153:287-288(1995) [PubMed: 7875606] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-69; 105-207 AND 560-599. Tissue: Liver.
[7]	"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry." Bunkenborg J., Pilch B.J., Podtelejnikov A.V., Wisniewski J.R. Proteomics 4:454-465(2004) [PubMed: 14760718] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-402, MASS SPECTROMETRY. Tissue: Plasma.
[8]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-402 AND ASN-488, MASS SPECTROMETRY. Tissue: Plasma.
[9]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33, MASS SPECTROMETRY. Tissue: Saliva.
[10]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-402, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L32140 mRNA. Translation: AAA21612.1. U51243 Genomic DNA. Translation: AAC50720.1. AK290556 mRNA. Translation: BAF83245.1. CH471057 Genomic DNA. Translation: EAX05682.1. BC109020 mRNA. Translation: AAI09021.1. BC109021 mRNA. Translation: AAI09022.1. L35486 mRNA. Translation: AAA68197.1. L35497 mRNA. Translation: AAA68198.1. L35498 mRNA. Translation: AAA68199.1.
IPI	IPI00019943.
PIR	A54906. I39424. I39426.
RefSeq	NP_001124.1.
UniGene	Hs.168718
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P43652.
PTM databases
PhosphoSite	P43652.
Proteomic databases
PeptideAtlas	P43652.
PRIDE	P43652.
Genome annotation databases
Ensembl	ENST00000226355; ENSP00000226355; ENSG00000079557; Homo sapiens. [Genome view]
GeneID	173.
KEGG	hsa:173.
UCSC	uc003hhb.1. human.
Organism-specific databases
CTD	173.
GeneCards	GC04P074587.
H-InvDB	HIX0031507.
HGNC	HGNC:316. AFM.
HPA	HPA017006.
MIM	104145. gene.
PharmGKB	PA24613.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG283181.
HOVERGEN	P43652.
InParanoid	P43652.
OMA	EFACVDN.
OrthoDB	EOG9962B0.
Gene expression databases
ArrayExpress	P43652.
Bgee	P43652.
CleanEx	HS_AFM.
Genevestigator	P43652.
GermOnline	ENSG00000079557. Homo sapiens.
Family and domain databases
InterPro	IPR001703. Alpha-fetoprotein. IPR000264. Serum_albumin. IPR020858. Serum_albumin-like. IPR020857. Serum_albumin_CS. IPR014760. Serum_albumin_N. [Graphical view]
Pfam	PF00273. Serum_albumin. 3 hits. [Graphical view]
PRINTS	PR00803. AFETOPROTEIN. PR00802. SERUMALBUMIN.
SMART	SM00103. ALBUMIN. 3 hits. [Graphical view]
PROSITE	PS00212. ALBUMIN_1. 3 hits. PS51438. ALBUMIN_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	694.
SOURCE	Search...

Entry information

Entry name

AFAM_HUMAN

Accession

Primary (citable) accession number: P43652
Secondary accession number(s): A8K3E1, Q32MR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.