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Protein

Afamin

Gene

AFM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier.3 Publications

GO - Molecular functioni

  • vitamin E binding Source: UniProtKB

GO - Biological processi

  • vitamin transport Source: UniProtKB

Keywordsi

Biological processTransport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:AFM
Synonyms:ALB2, ALBA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:316. AFM.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular region Source: ProtInc
  • extracellular space Source: UniProtKB

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi173.
OpenTargetsiENSG00000079557.
PharmGKBiPA24613.

Polymorphism and mutation databases

BioMutaiAFM.
DMDMi1168366.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000000110622 – 599AfaminAdd BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) (complex) asparagine4 Publications1
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation
Glycosylationi109N-linked (GlcNAc...) (complex) asparagine1 Publication1
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Disulfide bondi192 ↔ 201PROSITE-ProRule annotation
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Glycosylationi383N-linked (GlcNAc...) (complex) asparagine; atypical2 Publications1
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Glycosylationi402N-linked (GlcNAc...) (complex) asparagine5 Publications1
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation
Glycosylationi488N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation
Disulfide bondi536 ↔ 581PROSITE-ProRule annotation
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.7 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43652.
PeptideAtlasiP43652.
PRIDEiP43652.

PTM databases

iPTMnetiP43652.
PhosphoSitePlusiP43652.
UniCarbKBiP43652.

Expressioni

Tissue specificityi

High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000079557.
CleanExiHS_AFM.
GenevisibleiP43652. HS.

Organism-specific databases

HPAiHPA017006.

Interactioni

Protein-protein interaction databases

BioGridi106681. 4 interactors.
STRINGi9606.ENSP00000226355.

Structurei

3D structure databases

ProteinModelPortaliP43652.
SMRiP43652.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 210Albumin 1PROSITE-ProRule annotationAdd BLAST189
Domaini211 – 403Albumin 2PROSITE-ProRule annotationAdd BLAST193
Domaini404 – 599Albumin 3PROSITE-ProRule annotationAdd BLAST196

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIRZ. Eukaryota.
ENOG410Z40H. LUCA.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP43652.
OMAiLDPEEKC.
OrthoDBiEOG091G0F5F.
PhylomeDBiP43652.
TreeFamiTF335561.

Family and domain databases

CDDicd00015. ALBUMIN. 3 hits.
InterProiView protein in InterPro
IPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP/Afamin.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
PANTHERiPTHR11385. PTHR11385. 1 hit.
PfamiView protein in Pfam
PF00273. Serum_albumin. 3 hits.
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00803. AFETOPROTEIN.
PR00802. SERUMALBUMIN.
SMARTiView protein in SMART
SM00103. ALBUMIN. 3 hits.
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiView protein in PROSITE
PS00212. ALBUMIN_1. 3 hits.
PS51438. ALBUMIN_2. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLKLTGFI FFLFFLTESL TLPTQPRDIE NFNSTQKFIE DNIEYITIIA
60 70 80 90 100
FAQYVQEATF EEMEKLVKDM VEYKDRCMAD KTLPECSKLP NNVLQEKICA
110 120 130 140 150
MEGLPQKHNF SHCCSKVDAQ RRLCFFYNKK SDVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YESNRESLLN HFLYEVARRN PFVFAPTLLT VAVHFEEVAK SCCEEQNKVN
210 220 230 240 250
CLQTRAIPVT QYLKAFSSYQ KHVCGALLKF GTKVVHFIYI AILSQKFPKI
260 270 280 290 300
EFKELISLVE DVSSNYDGCC EGDVVQCIRD TSKVMNHICS KQDSISSKIK
310 320 330 340 350
ECCEKKIPER GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF
360 370 380 390 400
FAKFTFEYSR RHPDLSIPEL LRIVQIYKDL LRNCCNTENP PGCYRYAEDK
410 420 430 440 450
FNETTEKSLK MVQQECKHFQ NLGKDGLKYH YLIRLTKIAP QLSTEELVSL
460 470 480 490 500
GEKMVTAFTT CCTLSEEFAC VDNLADLVFG ELCGVNENRT INPAVDHCCK
510 520 530 540 550
TNFAFRRPCF ESLKADKTYV PPPFSQDLFT FHADMCQSQN EELQRKTDRF
560 570 580 590
LVNLVKLKHE LTDEELQSLF TNFANVVDKC CKAESPEVCF NEESPKIGN
Length:599
Mass (Da):69,069
Last modified:November 1, 1995 - v1
Checksum:iD594E75E20D308AB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061003395R → H. Corresponds to variant dbSNP:rs41265665Ensembl.1
Natural variantiVAR_048218404T → S. Corresponds to variant dbSNP:rs2276444Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32140 mRNA. Translation: AAA21612.1.
U51243 Genomic DNA. Translation: AAC50720.1.
AK290556 mRNA. Translation: BAF83245.1.
AC108157 Genomic DNA. No translation available.
AC110752 Genomic DNA. Translation: AAY41051.1.
CH471057 Genomic DNA. Translation: EAX05682.1.
BC109020 mRNA. Translation: AAI09021.1.
BC109021 mRNA. Translation: AAI09022.1.
L35486 mRNA. Translation: AAA68197.1.
L35497 mRNA. Translation: AAA68198.1.
L35498 mRNA. Translation: AAA68199.1.
CCDSiCCDS3557.1.
PIRiA54906.
I39424.
I39426.
RefSeqiNP_001124.1. NM_001133.2.
UniGeneiHs.168718.

Genome annotation databases

EnsembliENST00000226355; ENSP00000226355; ENSG00000079557.
GeneIDi173.
KEGGihsa:173.
UCSCiuc003hhb.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAFAM_HUMAN
AccessioniPrimary (citable) accession number: P43652
Secondary accession number(s): A8K3E1, Q32MR3, Q4W5C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 10, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families