Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Afamin

Gene

AFM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed:26902720). Binds vitamin E (PubMed:15952736, PubMed:12463752). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed:15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed:19046407).Curated4 Publications

GO - Molecular functioni

  • vitamin E binding Source: UniProtKB

GO - Biological processi

  • protein stabilization Source: UniProtKB
  • protein transport within extracellular region Source: UniProtKB
  • vitamin transport Source: UniProtKB

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin1 Publication
Short name:
Alpha-Alb
Gene namesi
Name:AFM
Synonyms:ALB2, ALBA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000079557.4
HGNCiHGNC:316 AFM
MIMi104145 gene
neXtProtiNX_P43652

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi173
OpenTargetsiENSG00000079557
PharmGKBiPA24613

Polymorphism and mutation databases

BioMutaiAFM
DMDMi1168366

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000000110622 – 599AfaminAdd BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) (complex) asparagine4 Publications1
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation1 Publication
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation1 Publication
Glycosylationi109N-linked (GlcNAc...) (complex) asparagine1 Publication1
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Disulfide bondi192 ↔ 201PROSITE-ProRule annotation1 Publication
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation1 Publication
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation1 Publication
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation1 Publication
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation1 Publication
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation1 Publication
Glycosylationi383N-linked (GlcNAc...) (complex) asparagine; atypical2 Publications1
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation1 Publication
Glycosylationi402N-linked (GlcNAc...) (complex) asparagine6 Publications1
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation1 Publication
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation1 Publication
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation1 Publication
Glycosylationi488N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation1 Publication
Disulfide bondi536 ↔ 581PROSITE-ProRule annotation1 Publication
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.8 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43652
PeptideAtlasiP43652
PRIDEiP43652
ProteomicsDBi55647

PTM databases

GlyConnecti731
iPTMnetiP43652
PhosphoSitePlusiP43652
UniCarbKBiP43652

Expressioni

Tissue specificityi

High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000079557
CleanExiHS_AFM
GenevisibleiP43652 HS

Organism-specific databases

HPAiHPA017006
HPA052437

Interactioni

Subunit structurei

Forms a 1:1 complex with Wnt family members; interacts with WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B.1 Publication

Protein-protein interaction databases

BioGridi106681, 5 interactors
STRINGi9606.ENSP00000226355

Structurei

Secondary structure

1599
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 41Combined sources9
Helixi43 – 54Combined sources12
Helixi60 – 78Combined sources19
Helixi85 – 87Combined sources3
Helixi90 – 99Combined sources10
Helixi104 – 107Combined sources4
Helixi111 – 114Combined sources4
Helixi118 – 128Combined sources11
Helixi157 – 169Combined sources13
Helixi175 – 190Combined sources16
Helixi198 – 203Combined sources6
Helixi207 – 230Combined sources24
Helixi232 – 246Combined sources15
Helixi252 – 271Combined sources20
Helixi274 – 290Combined sources17
Helixi291 – 294Combined sources4
Helixi300 – 303Combined sources4
Helixi309 – 316Combined sources8
Helixi331 – 333Combined sources3
Helixi339 – 345Combined sources7
Helixi347 – 360Combined sources14
Helixi367 – 384Combined sources18
Helixi390 – 394Combined sources5
Helixi397 – 438Combined sources42
Helixi444 – 461Combined sources18
Helixi467 – 483Combined sources17
Beta strandi485 – 487Combined sources3
Helixi493 – 501Combined sources9
Helixi506 – 511Combined sources6
Helixi526 – 529Combined sources4
Helixi533 – 535Combined sources3
Helixi541 – 557Combined sources17
Turni558 – 560Combined sources3
Helixi563 – 580Combined sources18
Beta strandi583 – 585Combined sources3
Helixi586 – 593Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OKLX-ray2.09A/B22-599[»]
ProteinModelPortaliP43652
SMRiP43652
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 210Albumin 1PROSITE-ProRule annotationAdd BLAST189
Domaini211 – 403Albumin 2PROSITE-ProRule annotationAdd BLAST193
Domaini404 – 599Albumin 3PROSITE-ProRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 319Binding pocket for hydrophobic ligands1 PublicationAdd BLAST105

Domaini

The second albumin domain forms a deep binding pocket that contains palmitoleic acid (in vitro) (PubMed:29153507). Palmitoleic acid is most likely not the physiological ligand. Instead, this pocket may accomodate the covalently bound lipid moiety of Wnt family members (Probable).Curated1 Publication

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIRZ Eukaryota
ENOG410Z40H LUCA
GeneTreeiENSGT00390000000113
HOGENOMiHOG000293137
HOVERGENiHBG004207
InParanoidiP43652
OMAiLDPEEKC
OrthoDBiEOG091G0F5F
PhylomeDBiP43652
TreeFamiTF335561

Family and domain databases

CDDicd00015 ALBUMIN, 3 hits
InterProiView protein in InterPro
IPR000264 ALB/AFP/VDB
IPR020858 Serum_albumin-like
IPR021177 Serum_albumin/AFP/Afamin
IPR020857 Serum_albumin_CS
IPR014760 Serum_albumin_N
PANTHERiPTHR11385 PTHR11385, 1 hit
PfamiView protein in Pfam
PF00273 Serum_albumin, 3 hits
PIRSFiPIRSF002520 Serum_albumin_subgroup, 1 hit
PRINTSiPR00803 AFETOPROTEIN
PR00802 SERUMALBUMIN
SMARTiView protein in SMART
SM00103 ALBUMIN, 3 hits
SUPFAMiSSF48552 SSF48552, 3 hits
PROSITEiView protein in PROSITE
PS00212 ALBUMIN_1, 3 hits
PS51438 ALBUMIN_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLKLTGFI FFLFFLTESL TLPTQPRDIE NFNSTQKFIE DNIEYITIIA
60 70 80 90 100
FAQYVQEATF EEMEKLVKDM VEYKDRCMAD KTLPECSKLP NNVLQEKICA
110 120 130 140 150
MEGLPQKHNF SHCCSKVDAQ RRLCFFYNKK SDVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YESNRESLLN HFLYEVARRN PFVFAPTLLT VAVHFEEVAK SCCEEQNKVN
210 220 230 240 250
CLQTRAIPVT QYLKAFSSYQ KHVCGALLKF GTKVVHFIYI AILSQKFPKI
260 270 280 290 300
EFKELISLVE DVSSNYDGCC EGDVVQCIRD TSKVMNHICS KQDSISSKIK
310 320 330 340 350
ECCEKKIPER GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF
360 370 380 390 400
FAKFTFEYSR RHPDLSIPEL LRIVQIYKDL LRNCCNTENP PGCYRYAEDK
410 420 430 440 450
FNETTEKSLK MVQQECKHFQ NLGKDGLKYH YLIRLTKIAP QLSTEELVSL
460 470 480 490 500
GEKMVTAFTT CCTLSEEFAC VDNLADLVFG ELCGVNENRT INPAVDHCCK
510 520 530 540 550
TNFAFRRPCF ESLKADKTYV PPPFSQDLFT FHADMCQSQN EELQRKTDRF
560 570 580 590
LVNLVKLKHE LTDEELQSLF TNFANVVDKC CKAESPEVCF NEESPKIGN
Length:599
Mass (Da):69,069
Last modified:November 1, 1995 - v1
Checksum:iD594E75E20D308AB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061003395R → H. Corresponds to variant dbSNP:rs41265665Ensembl.1
Natural variantiVAR_048218404T → S. Corresponds to variant dbSNP:rs2276444Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32140 mRNA Translation: AAA21612.1
U51243 Genomic DNA Translation: AAC50720.1
AK290556 mRNA Translation: BAF83245.1
AC108157 Genomic DNA No translation available.
AC110752 Genomic DNA Translation: AAY41051.1
CH471057 Genomic DNA Translation: EAX05682.1
BC109020 mRNA Translation: AAI09021.1
BC109021 mRNA Translation: AAI09022.1
L35486 mRNA Translation: AAA68197.1
L35497 mRNA Translation: AAA68198.1
L35498 mRNA Translation: AAA68199.1
CCDSiCCDS3557.1
PIRiA54906
I39424
I39426
RefSeqiNP_001124.1, NM_001133.2
UniGeneiHs.168718

Genome annotation databases

EnsembliENST00000226355; ENSP00000226355; ENSG00000079557
GeneIDi173
KEGGihsa:173
UCSCiuc003hhb.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAFAM_HUMAN
AccessioniPrimary (citable) accession number: P43652
Secondary accession number(s): A8K3E1, Q32MR3, Q4W5C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health