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P43642

- T2MU_MYCSP

UniProt

P43642 - T2MU_MYCSP

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Protein
Type-2 restriction enzyme MunI
Gene
munIR
Organism
Mycoplasma sp.
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CAATTG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1285. MunI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MunI (EC:3.1.21.4)
Short name:
R.MunI
Alternative name(s):
Endonuclease MunI
Type II restriction enzyme MunI
Gene namesi
Name:munIR
OrganismiMycoplasma sp.
Taxonomic identifieri2108 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Type-2 restriction enzyme MunI
PRO_0000077341Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 209
Helixi27 – 348
Turni35 – 373
Beta strandi38 – 458
Beta strandi50 – 534
Helixi60 – 656
Helixi73 – 753
Beta strandi83 – 886
Turni89 – 913
Beta strandi94 – 1018
Helixi111 – 1144
Helixi118 – 1269
Helixi128 – 13811
Beta strandi147 – 1548
Helixi155 – 1584
Helixi160 – 17011
Beta strandi176 – 1805
Helixi186 – 19611
Helixi198 – 2014

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D02X-ray1.70A/B1-202[»]
ProteinModelPortaliP43642.
SMRiP43642. Positions 3-202.

Miscellaneous databases

EvolutionaryTraceiP43642.

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view]
PfamiPF11407. RestrictionMunI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P43642-1 [UniParc]FASTAAdd to Basket

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MGKSELSGRL NWQALAGLKA SGAEQNLYNV FNAVFEGTKY VLYEKPKHLK    50
NLYAQVVLPD DVIKEIFNPL IDLSTTQWGV SPDFAIENTE THKILFGEIK 100
RQDGWVEGKD PSAGRGNAHE RSCKLFTPGL LKAYRTIGGI NDEEILPFWV 150
VFEGDITRDP KRVREITFWY DHYQDNYFMW RPNESGEKLV QHFNEKLKKY 200
LD 202
Length:202
Mass (Da):23,389
Last modified:November 1, 1995 - v1
Checksum:i7964F7C8EBFEC469
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76192 Genomic DNA. Translation: CAA53788.1.
PIRiS38901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76192 Genomic DNA. Translation: CAA53788.1 .
PIRi S38901.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D02 X-ray 1.70 A/B 1-202 [» ]
ProteinModelPortali P43642.
SMRi P43642. Positions 3-202.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1285. MunI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P43642.

Family and domain databases

Gene3Di 3.40.580.10. 1 hit.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view ]
Pfami PF11407. RestrictionMunI. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI."
    Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.
    Gene 142:1-8(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution."
    Deibert M., Grazulis S., Janulaitis A., Siksnys V., Huber R.
    EMBO J. 18:5805-5816(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiT2MU_MYCSP
AccessioniPrimary (citable) accession number: P43642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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