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Protein

Type-2 restriction enzyme MunI

Gene

munIR

Organism
Mycoplasma sp.
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CAATTG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1285. MunI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MunI (EC:3.1.21.4)
Short name:
R.MunI
Alternative name(s):
Endonuclease MunI
Type II restriction enzyme MunI
Gene namesi
Name:munIR
OrganismiMycoplasma sp.
Taxonomic identifieri2108 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773411 – 202Type-2 restriction enzyme MunIAdd BLAST202

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 20Combined sources9
Helixi27 – 34Combined sources8
Turni35 – 37Combined sources3
Beta strandi38 – 45Combined sources8
Beta strandi50 – 53Combined sources4
Helixi60 – 65Combined sources6
Helixi73 – 75Combined sources3
Beta strandi83 – 88Combined sources6
Turni89 – 91Combined sources3
Beta strandi94 – 101Combined sources8
Helixi111 – 114Combined sources4
Helixi118 – 126Combined sources9
Helixi128 – 138Combined sources11
Beta strandi147 – 154Combined sources8
Helixi155 – 158Combined sources4
Helixi160 – 170Combined sources11
Beta strandi176 – 180Combined sources5
Helixi186 – 196Combined sources11
Helixi198 – 201Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D02X-ray1.70A/B1-202[»]
ProteinModelPortaliP43642.
SMRiP43642.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43642.

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view]
PfamiPF11407. RestrictionMunI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P43642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSELSGRL NWQALAGLKA SGAEQNLYNV FNAVFEGTKY VLYEKPKHLK
60 70 80 90 100
NLYAQVVLPD DVIKEIFNPL IDLSTTQWGV SPDFAIENTE THKILFGEIK
110 120 130 140 150
RQDGWVEGKD PSAGRGNAHE RSCKLFTPGL LKAYRTIGGI NDEEILPFWV
160 170 180 190 200
VFEGDITRDP KRVREITFWY DHYQDNYFMW RPNESGEKLV QHFNEKLKKY

LD
Length:202
Mass (Da):23,389
Last modified:November 1, 1995 - v1
Checksum:i7964F7C8EBFEC469
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76192 Genomic DNA. Translation: CAA53788.1.
PIRiS38901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76192 Genomic DNA. Translation: CAA53788.1.
PIRiS38901.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D02X-ray1.70A/B1-202[»]
ProteinModelPortaliP43642.
SMRiP43642.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1285. MunI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP43642.

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view]
PfamiPF11407. RestrictionMunI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2MU_MYCSP
AccessioniPrimary (citable) accession number: P43642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.