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P43642

- T2MU_MYCSP

UniProt

P43642 - T2MU_MYCSP

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Protein

Type-2 restriction enzyme MunI

Gene

munIR

Organism
Mycoplasma sp.
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CAATTG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1285. MunI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MunI (EC:3.1.21.4)
Short name:
R.MunI
Alternative name(s):
Endonuclease MunI
Type II restriction enzyme MunI
Gene namesi
Name:munIR
OrganismiMycoplasma sp.
Taxonomic identifieri2108 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Type-2 restriction enzyme MunIPRO_0000077341Add
BLAST

Structurei

Secondary structure

1
202
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 209
Helixi27 – 348
Turni35 – 373
Beta strandi38 – 458
Beta strandi50 – 534
Helixi60 – 656
Helixi73 – 753
Beta strandi83 – 886
Turni89 – 913
Beta strandi94 – 1018
Helixi111 – 1144
Helixi118 – 1269
Helixi128 – 13811
Beta strandi147 – 1548
Helixi155 – 1584
Helixi160 – 17011
Beta strandi176 – 1805
Helixi186 – 19611
Helixi198 – 2014

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D02X-ray1.70A/B1-202[»]
ProteinModelPortaliP43642.
SMRiP43642. Positions 3-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43642.

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view]
PfamiPF11407. RestrictionMunI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P43642-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKSELSGRL NWQALAGLKA SGAEQNLYNV FNAVFEGTKY VLYEKPKHLK
60 70 80 90 100
NLYAQVVLPD DVIKEIFNPL IDLSTTQWGV SPDFAIENTE THKILFGEIK
110 120 130 140 150
RQDGWVEGKD PSAGRGNAHE RSCKLFTPGL LKAYRTIGGI NDEEILPFWV
160 170 180 190 200
VFEGDITRDP KRVREITFWY DHYQDNYFMW RPNESGEKLV QHFNEKLKKY

LD
Length:202
Mass (Da):23,389
Last modified:November 1, 1995 - v1
Checksum:i7964F7C8EBFEC469
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76192 Genomic DNA. Translation: CAA53788.1.
PIRiS38901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76192 Genomic DNA. Translation: CAA53788.1 .
PIRi S38901.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D02 X-ray 1.70 A/B 1-202 [» ]
ProteinModelPortali P43642.
SMRi P43642. Positions 3-202.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1285. MunI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P43642.

Family and domain databases

Gene3Di 3.40.580.10. 1 hit.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view ]
Pfami PF11407. RestrictionMunI. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI."
    Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.
    Gene 142:1-8(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution."
    Deibert M., Grazulis S., Janulaitis A., Siksnys V., Huber R.
    EMBO J. 18:5805-5816(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiT2MU_MYCSP
AccessioniPrimary (citable) accession number: P43642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3