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P43642 (T2MU_MYCSP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MunI

Short name=R.MunI
EC=3.1.21.4
Alternative name(s):
Endonuclease MunI
Type II restriction enzyme MunI
Gene names
Name:munIR
OrganismMycoplasma sp.
Taxonomic identifier2108 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CAATTG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202Type-2 restriction enzyme MunI
PRO_0000077341

Secondary structure

................................... 202
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43642 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7964F7C8EBFEC469

FASTA20223,389
        10         20         30         40         50         60 
MGKSELSGRL NWQALAGLKA SGAEQNLYNV FNAVFEGTKY VLYEKPKHLK NLYAQVVLPD 

        70         80         90        100        110        120 
DVIKEIFNPL IDLSTTQWGV SPDFAIENTE THKILFGEIK RQDGWVEGKD PSAGRGNAHE 

       130        140        150        160        170        180 
RSCKLFTPGL LKAYRTIGGI NDEEILPFWV VFEGDITRDP KRVREITFWY DHYQDNYFMW 

       190        200 
RPNESGEKLV QHFNEKLKKY LD 

« Hide

References

[1]"CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI."
Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.
Gene 142:1-8(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution."
Deibert M., Grazulis S., Janulaitis A., Siksnys V., Huber R.
EMBO J. 18:5805-5816(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76192 Genomic DNA. Translation: CAA53788.1.
PIRS38901.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D02X-ray1.70A/B1-202[»]
ProteinModelPortalP43642.
SMRP43642. Positions 3-202.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1285. MunI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.580.10. 1 hit.
InterProIPR011335. Restrct_endonuc-II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view]
PfamPF11407. RestrictionMunI. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP43642.

Entry information

Entry nameT2MU_MYCSP
AccessionPrimary (citable) accession number: P43642
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references