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P43642

- T2MU_MYCSP

UniProt

P43642 - T2MU_MYCSP

Protein

Type-2 restriction enzyme MunI

Gene

munIR

Organism
Mycoplasma sp.
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (16 Apr 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CAATTG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1285. MunI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MunI (EC:3.1.21.4)
    Short name:
    R.MunI
    Alternative name(s):
    Endonuclease MunI
    Type II restriction enzyme MunI
    Gene namesi
    Name:munIR
    OrganismiMycoplasma sp.
    Taxonomic identifieri2108 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 202202Type-2 restriction enzyme MunIPRO_0000077341Add
    BLAST

    Structurei

    Secondary structure

    1
    202
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 209
    Helixi27 – 348
    Turni35 – 373
    Beta strandi38 – 458
    Beta strandi50 – 534
    Helixi60 – 656
    Helixi73 – 753
    Beta strandi83 – 886
    Turni89 – 913
    Beta strandi94 – 1018
    Helixi111 – 1144
    Helixi118 – 1269
    Helixi128 – 13811
    Beta strandi147 – 1548
    Helixi155 – 1584
    Helixi160 – 17011
    Beta strandi176 – 1805
    Helixi186 – 19611
    Helixi198 – 2014

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1D02X-ray1.70A/B1-202[»]
    ProteinModelPortaliP43642.
    SMRiP43642. Positions 3-202.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43642.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.580.10. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR011336. Restrct_endonuc_II_EcoRI/MunI.
    IPR022725. Restrct_endonuc_II_MunI.
    [Graphical view]
    PfamiPF11407. RestrictionMunI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43642-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKSELSGRL NWQALAGLKA SGAEQNLYNV FNAVFEGTKY VLYEKPKHLK    50
    NLYAQVVLPD DVIKEIFNPL IDLSTTQWGV SPDFAIENTE THKILFGEIK 100
    RQDGWVEGKD PSAGRGNAHE RSCKLFTPGL LKAYRTIGGI NDEEILPFWV 150
    VFEGDITRDP KRVREITFWY DHYQDNYFMW RPNESGEKLV QHFNEKLKKY 200
    LD 202
    Length:202
    Mass (Da):23,389
    Last modified:November 1, 1995 - v1
    Checksum:i7964F7C8EBFEC469
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76192 Genomic DNA. Translation: CAA53788.1.
    PIRiS38901.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76192 Genomic DNA. Translation: CAA53788.1 .
    PIRi S38901.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1D02 X-ray 1.70 A/B 1-202 [» ]
    ProteinModelPortali P43642.
    SMRi P43642. Positions 3-202.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1285. MunI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P43642.

    Family and domain databases

    Gene3Di 3.40.580.10. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR011336. Restrct_endonuc_II_EcoRI/MunI.
    IPR022725. Restrct_endonuc_II_MunI.
    [Graphical view ]
    Pfami PF11407. RestrictionMunI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI."
      Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.
      Gene 142:1-8(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution."
      Deibert M., Grazulis S., Janulaitis A., Siksnys V., Huber R.
      EMBO J. 18:5805-5816(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

    Entry informationi

    Entry nameiT2MU_MYCSP
    AccessioniPrimary (citable) accession number: P43642
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: April 16, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3