P43642 (T2MU_MYCSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme MunI
Short name=R.MunI
EC=3.1.21.4

Alternative name(s):
Endonuclease MunI
Type II restriction enzyme MunI

Gene names

Name:

munIR

Organism

Mycoplasma sp.

Taxonomic identifier

2108 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded sequence CAATTG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 202

202

Type-2 restriction enzyme MunI

PRO_0000077341

Secondary structure

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202

Helix Strand Turn

Details...

Helix

12 – 20

9

Helix

27 – 34

8

Turn

35 – 37

3

Beta strand

38 – 45

8

Beta strand

50 – 53

4

Helix

60 – 65

6

Helix

73 – 75

3

Beta strand

83 – 88

6

Turn

89 – 91

3

Beta strand

94 – 101

8

Helix

111 – 114

4

Helix

118 – 126

9

Helix

128 – 138

11

Beta strand

147 – 154

8

Helix

155 – 158

4

Helix

160 – 170

11

Beta strand

176 – 180

5

Helix

186 – 196

11

Helix

198 – 201

4

Sequences

	Sequence		Length	Mass (Da)
	P43642 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7964F7C8EBFEC469	FASTA	202	23,389
	10 20 30 40 50 60 MGKSELSGRL NWQALAGLKA SGAEQNLYNV FNAVFEGTKY VLYEKPKHLK NLYAQVVLPD 70 80 90 100 110 120 DVIKEIFNPL IDLSTTQWGV SPDFAIENTE THKILFGEIK RQDGWVEGKD PSAGRGNAHE 130 140 150 160 170 180 RSCKLFTPGL LKAYRTIGGI NDEEILPFWV VFEGDITRDP KRVREITFWY DHYQDNYFMW 190 200 RPNESGEKLV QHFNEKLKKY LD

References

[1]	"CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI." Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A. Gene 142:1-8(1994) [PubMed: 8181741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution." Deibert M., Grazulis S., Janulaitis A., Siksnys V., Huber R. EMBO J. 18:5805-5816(1999) [PubMed: 10545092] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X76192 Genomic DNA. Translation: CAA53788.1.

PIR

S38901.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D02	X-ray	1.70	A/B	1-202	[»]

ProteinModelPortal

P43642.

SMR

P43642. Positions 3-202.

ModBase

Search...

Protein family/group databases

REBASE

1285. MunI.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR011335. Restrct_endonuc_II-like.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR022725. Restrct_endonuc_II_MunI.
[Graphical view]

Gene3D

G3DSA:3.40.580.10. Restrict_endonuc_II_EcoRI/MunI. 1 hit.

Pfam

PF11407. RestrictionMunI. 1 hit.
[Graphical view]

SUPFAM

SSF52980. Restrict_endonuc_II-like_core. 1 hit.

ProtoNet

Search...

Entry information

Entry name

T2MU_MYCSP

Accession

Primary (citable) accession number: P43642

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 11, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program