P43641 (MTMU_MYCSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase MunI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase MunI
Short name=M.MunI

Gene names

Name:

munIM

Organism

Mycoplasma sp.

Taxonomic identifier

2108 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the MT-A70-like family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Modification methylase MunI		PRO_0000207637

Sequences

	Sequence		Length	Mass (Da)
	P43641 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0BE21A05BB2A3A2E	FASTA	233	26,933
	10 20 30 40 50 60 MENKVTAYSI YNKKAKKNTK VNPLDEVFPQ LPRKKYQVIY ADPPWDYGGK MQYDKSTIKS 70 80 90 100 110 120 ENEGFKRDIF ISSASFKYPT LKLKELQQLD VPSITADDCI LFMWTTGPQM ANSILLGESW 130 140 150 160 170 180 GFEYKTVAFV WDKMVHNPGR YTLSQTEFVL VFKKGKIPTP RGARNVRQLL QIHRGQHSEK 190 200 210 220 230 PYAVIDGITK MFPALDKIEL FARNNFVGWD NWGLEIPDNK IEIPTQGEID ENK

References

[1]

"CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI." Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A. Gene 142:1-8(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76192 Genomic DNA. Translation: CAA53786.1.
PIR	S38899.
3D structure databases
ProteinModelPortal	P43641.
ModBase	Search...
Protein family/group databases
REBASE	3449. M.MunI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR007757. MT-A70-like. [Graphical view]
Pfam	PF05063. MT-A70. 1 hit. [Graphical view]
PROSITE	PS51143. MT_A70. 1 hit. PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTMU_MYCSP

Accession

Primary (citable) accession number: P43641

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program