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P43641 (MTMU_MYCSP) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Modification methylase MunI

EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase MunI
Short name=M.MunI
Gene names
Name:munIM
OrganismMycoplasma sp.
Taxonomic identifier2108 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the MT-A70-like family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233Modification methylase MunI
PRO_0000207637

Sequences

Sequence LengthMass (Da)Tools
P43641 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0BE21A05BB2A3A2E

FASTA23326,933
        10         20         30         40         50         60 
MENKVTAYSI YNKKAKKNTK VNPLDEVFPQ LPRKKYQVIY ADPPWDYGGK MQYDKSTIKS 

        70         80         90        100        110        120 
ENEGFKRDIF ISSASFKYPT LKLKELQQLD VPSITADDCI LFMWTTGPQM ANSILLGESW 

       130        140        150        160        170        180 
GFEYKTVAFV WDKMVHNPGR YTLSQTEFVL VFKKGKIPTP RGARNVRQLL QIHRGQHSEK 

       190        200        210        220        230 
PYAVIDGITK MFPALDKIEL FARNNFVGWD NWGLEIPDNK IEIPTQGEID ENK 

« Hide

References

[1]"CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI."
Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.
Gene 142:1-8(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76192 Genomic DNA. Translation: CAA53786.1.
PIRS38899.

3D structure databases

ProteinModelPortalP43641.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3449. M.MunI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51143. MT_A70. 1 hit.
PS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTMU_MYCSP
AccessionPrimary (citable) accession number: P43641
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries