P43639 (CSK2B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Casein kinase II subunit beta
Short name=CK II beta

Gene names

Name:	CKB1
Ordered Locus Names:	YGL019W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a complex role in regulating the basal catalytic activity of the alpha subunit By similarity.
Subunit structure	Tetramer composed of an alpha subunit, an alpha' subunit, one beta subunit and one beta' subunit. Interacts with FACT subunits POB3 and SPT16. Ref.5
Post-translational modification	Phosphorylated by alpha subunit By similarity.
Miscellaneous	Present with 8120 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the casein kinase 2 subunit beta family.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein phosphorylation Inferred from direct assay PubMed 16952051. Source: SGD regulation of transcription from RNA polymerase I promoter Inferred from direct assay PubMed 11551505. Source: SGD regulation of transcription from RNA polymerase III promoter Inferred from direct assay PubMed 11551505. Source: SGD response to DNA damage stimulus Inferred from direct assay PubMed 11551505. Source: SGD
Cellular_component	UTP-C complex Inferred from direct assay PubMed 17515605. Source: SGD protein kinase CK2 complex Inferred from direct assay Ref.4. Source: SGD
Molecular_function	protein serine/threonine kinase inhibitor activity Inferred from direct assay PubMed 18265947. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CKA1	P15790	9	EBI-9563,EBI-9533
CKA2	P19454	7	EBI-9563,EBI-9548
CKB2	P38930	7	EBI-9563,EBI-9578

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Casein kinase II subunit beta		PRO_0000068257
Amino acid modifications
	Modified residue	111	1	Phosphoserine Ref.7
	Modified residue	260	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P43639 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9ACA8D285E6990AF	FASTA	278	32,265
	10 20 30 40 50 60 MSQEFVEDYS RTGSSDDEDS GAYDEWIPSF CSRFGHEYFC QVPTEFIEDD FNMTSLSQEV 70 80 90 100 110 120 PHYRKALDLI LDLEAMSDEE EDEDDVVEED EVDQEMQSND GHDEGKRRNK SPVVNKSIIE 130 140 150 160 170 180 HAAEQLYGLI HARFILTKPG LQAMAEKFDH KEFGTCPRYY CNGMQLLPCG LSDTVGKHTV 190 200 210 220 230 240 RLYCPSCQDL YLPQSSRFLC LEGAFWGTSF PGVFLKHFKE LEEYVERKSK ESYELKVFGF 250 260 270 RINDEAVSGP RMKWLRQYPS TEEDWEEFAK CEFETPAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and disruption of CKB1, the gene encoding the 38-kDa beta subunit of Saccharomyces cerevisiae casein kinase II (CKII). Deletion of CKII regulatory subunits elicits a salt-sensitive phenotype." Bidwai A.P., Reed J.C., Glover C.V.C. J. Biol. Chem. 270:10395-10404(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Casein kinase II of Saccharomyces cerevisiae contains two distinct regulatory subunits, beta and beta'." Bidwai A.P., Reed J.C., Glover C.V.C. Arch. Biochem. Biophys. 309:348-355(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-52; 182-196 AND 257-270.
[5]	"RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach." Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F. Mol. Cell. Biol. 22:6979-6992(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH POB3 AND SPT16.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-260, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U21283 Genomic DNA. Translation: AAA86829.1. Z72541 Genomic DNA. Translation: CAA96719.1. BK006941 Genomic DNA. Translation: DAA08079.1.
PIR	A56421.
RefSeq	NP_011496.3. NM_001180884.3.
3D structure databases
ProteinModelPortal	P43639.
SMR	P43639. Positions 26-246.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-282N.
IntAct	P43639. 58 interactions.
MINT	MINT-396457.
STRING	4932.YGL019W.
Proteomic databases
PaxDb	P43639.
PeptideAtlas	P43639.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL019W; YGL019W; YGL019W.
GeneID	852865.
KEGG	sce:YGL015C. sce:YGL019W.
Organism-specific databases
CYGD	YGL019w.
SGD	S000002987. CKB1.
Phylogenomic databases
eggNOG	COG5041.
HOGENOM	HOG000039270.
KO	K03115.
OMA	CSRFGHE.
OrthoDB	EOG44TSHZ.
Gene expression databases
Genevestigator	P43639.
GermOnline	YGL019W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.1820.10. 1 hit. 2.20.25.20. 1 hit.
InterPro	IPR016149. Casein_kin_II_reg-sub_a-hlx. IPR016150. Casein_kin_II_reg-sub_b-sht. IPR000704. Casein_kinase_II_reg-sub. [Graphical view]
PANTHER	PTHR11740. PTHR11740. 1 hit.
Pfam	PF01214. CK_II_beta. 1 hit. [Graphical view]
PRINTS	PR00472. CASNKINASEII.
SMART	SM01085. CK_II_beta. 1 hit. [Graphical view]
SUPFAM	SSF57798. Casein_kinase_II_reg-sub. 1 hit.
PROSITE	PS01101. CK2_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972481.

Entry information

Entry name

CSK2B_YEAST

Accession

Primary (citable) accession number: P43639
Secondary accession number(s): D6VUB8, Q9URG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program