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P43639

- CSK2B_YEAST

UniProt

P43639 - CSK2B_YEAST

Protein

Casein kinase II subunit beta

Gene

CKB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Plays a complex role in regulating the basal catalytic activity of the alpha subunit.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase regulator activity Source: SGD
    3. protein serine/threonine kinase inhibitor activity Source: SGD

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: SGD
    2. negative regulation of protein serine/threonine kinase activity Source: GOC
    3. protein phosphorylation Source: SGD
    4. regulation of protein kinase activity Source: GOC
    5. regulation of transcription from RNA polymerase III promoter Source: SGD
    6. regulation of transcription from RNA polymerase I promoter Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30539-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Casein kinase II subunit beta
    Short name:
    CK II beta
    Gene namesi
    Name:CKB1
    Ordered Locus Names:YGL019W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL019w.
    SGDiS000002987. CKB1.

    Subcellular locationi

    GO - Cellular componenti

    1. protein kinase CK2 complex Source: SGD
    2. UTP-C complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 278277Casein kinase II subunit betaPRO_0000068257Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei2 – 21Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by alpha subunit.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP43639.
    PaxDbiP43639.
    PeptideAtlasiP43639.

    Expressioni

    Gene expression databases

    GenevestigatoriP43639.

    Interactioni

    Subunit structurei

    Tetramer composed of an alpha subunit, an alpha' subunit, one beta subunit and one beta' subunit. Interacts with FACT subunits POB3 and SPT16.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CKA1P157907EBI-9563,EBI-9533
    CKA2P194547EBI-9563,EBI-9548
    CKB2P389307EBI-9563,EBI-9578

    Protein-protein interaction databases

    BioGridi33227. 286 interactions.
    DIPiDIP-282N.
    IntActiP43639. 56 interactions.
    MINTiMINT-396457.
    STRINGi4932.YGL019W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43639.
    SMRiP43639. Positions 26-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5041.
    HOGENOMiHOG000039270.
    KOiK03115.
    OMAiGHDYFVE.
    OrthoDBiEOG7K6Q6G.

    Family and domain databases

    Gene3Di1.10.1820.10. 1 hit.
    2.20.25.20. 1 hit.
    InterProiIPR016149. Casein_kin_II_reg-sub_a-hlx.
    IPR016150. Casein_kin_II_reg-sub_b-sht.
    IPR000704. Casein_kinase_II_reg-sub.
    [Graphical view]
    PANTHERiPTHR11740. PTHR11740. 1 hit.
    PfamiPF01214. CK_II_beta. 1 hit.
    [Graphical view]
    PRINTSiPR00472. CASNKINASEII.
    SMARTiSM01085. CK_II_beta. 1 hit.
    [Graphical view]
    SUPFAMiSSF57798. SSF57798. 2 hits.
    PROSITEiPS01101. CK2_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43639-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQEFVEDYS RTGSSDDEDS GAYDEWIPSF CSRFGHEYFC QVPTEFIEDD    50
    FNMTSLSQEV PHYRKALDLI LDLEAMSDEE EDEDDVVEED EVDQEMQSND 100
    GHDEGKRRNK SPVVNKSIIE HAAEQLYGLI HARFILTKPG LQAMAEKFDH 150
    KEFGTCPRYY CNGMQLLPCG LSDTVGKHTV RLYCPSCQDL YLPQSSRFLC 200
    LEGAFWGTSF PGVFLKHFKE LEEYVERKSK ESYELKVFGF RINDEAVSGP 250
    RMKWLRQYPS TEEDWEEFAK CEFETPAV 278
    Length:278
    Mass (Da):32,265
    Last modified:November 1, 1995 - v1
    Checksum:i9ACA8D285E6990AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21283 Genomic DNA. Translation: AAA86829.1.
    Z72541 Genomic DNA. Translation: CAA96719.1.
    BK006941 Genomic DNA. Translation: DAA08079.1.
    PIRiA56421.
    RefSeqiNP_011496.3. NM_001180884.3.

    Genome annotation databases

    EnsemblFungiiYGL019W; YGL019W; YGL019W.
    GeneIDi852865.
    KEGGisce:YGL019W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21283 Genomic DNA. Translation: AAA86829.1 .
    Z72541 Genomic DNA. Translation: CAA96719.1 .
    BK006941 Genomic DNA. Translation: DAA08079.1 .
    PIRi A56421.
    RefSeqi NP_011496.3. NM_001180884.3.

    3D structure databases

    ProteinModelPortali P43639.
    SMRi P43639. Positions 26-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33227. 286 interactions.
    DIPi DIP-282N.
    IntActi P43639. 56 interactions.
    MINTi MINT-396457.
    STRINGi 4932.YGL019W.

    Proteomic databases

    MaxQBi P43639.
    PaxDbi P43639.
    PeptideAtlasi P43639.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL019W ; YGL019W ; YGL019W .
    GeneIDi 852865.
    KEGGi sce:YGL019W.

    Organism-specific databases

    CYGDi YGL019w.
    SGDi S000002987. CKB1.

    Phylogenomic databases

    eggNOGi COG5041.
    HOGENOMi HOG000039270.
    KOi K03115.
    OMAi GHDYFVE.
    OrthoDBi EOG7K6Q6G.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30539-MONOMER.

    Miscellaneous databases

    NextBioi 972481.

    Gene expression databases

    Genevestigatori P43639.

    Family and domain databases

    Gene3Di 1.10.1820.10. 1 hit.
    2.20.25.20. 1 hit.
    InterProi IPR016149. Casein_kin_II_reg-sub_a-hlx.
    IPR016150. Casein_kin_II_reg-sub_b-sht.
    IPR000704. Casein_kinase_II_reg-sub.
    [Graphical view ]
    PANTHERi PTHR11740. PTHR11740. 1 hit.
    Pfami PF01214. CK_II_beta. 1 hit.
    [Graphical view ]
    PRINTSi PR00472. CASNKINASEII.
    SMARTi SM01085. CK_II_beta. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57798. SSF57798. 2 hits.
    PROSITEi PS01101. CK2_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and disruption of CKB1, the gene encoding the 38-kDa beta subunit of Saccharomyces cerevisiae casein kinase II (CKII). Deletion of CKII regulatory subunits elicits a salt-sensitive phenotype."
      Bidwai A.P., Reed J.C., Glover C.V.C.
      J. Biol. Chem. 270:10395-10404(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Casein kinase II of Saccharomyces cerevisiae contains two distinct regulatory subunits, beta and beta'."
      Bidwai A.P., Reed J.C., Glover C.V.C.
      Arch. Biochem. Biophys. 309:348-355(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 34-52; 182-196 AND 257-270.
    5. "RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach."
      Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F.
      Mol. Cell. Biol. 22:6979-6992(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH POB3 AND SPT16.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCSK2B_YEAST
    AccessioniPrimary (citable) accession number: P43639
    Secondary accession number(s): D6VUB8, Q9URG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8120 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3