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Protein

Alpha-1,3/1,6-mannosyltransferase ALG2

Gene

ALG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.1 Publication

Catalytic activityi

GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.1 Publication
GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YGL065C-MONOMER.
YEAST:YGL065C-MONOMER.
BRENDAi2.4.1.132. 984.
2.4.1.257. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
Alternative name(s):
Asparagine-linked glycosylation protein 2
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene namesi
Name:ALG2
Ordered Locus Names:YGL065C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL065C.
SGDiS000003033. ALG2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Transmembranei443 – 463HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi335E → A: Severly affects activity, especially the second mannosylation step. 1 Publication1
Mutagenesisi343E → A: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802711 – 503Alpha-1,3/1,6-mannosyltransferase ALG2Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi170N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1
Glycosylationi371N-linked (GlcNAc...)Sequence analysis1
Glycosylationi400N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP43636.
PRIDEiP43636.

PTM databases

iPTMnetiP43636.

Interactioni

Subunit structurei

Interacts with ALG1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ALG1P166612EBI-2459,EBI-2206309

Protein-protein interaction databases

BioGridi33182. 7 interactors.
DIPiDIP-5401N.
IntActiP43636. 4 interactors.
MINTiMINT-523155.

Structurei

3D structure databases

ProteinModelPortaliP43636.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075033.
HOGENOMiHOG000177048.
InParanoidiP43636.
KOiK03843.
OMAiKTHCFEE.
OrthoDBiEOG092C1IJD.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKDKRTIA FIHPDLGIGG AERLVVDAAL GLQQQGHSVI IYTSHCDKSH
60 70 80 90 100
CFEEVKNGQL KVEVYGDFLP TNFLGRFFIV FATIRQLYLV IQLILQKKVN
110 120 130 140 150
AYQLIIIDQL STCIPLLHIF SSATLMFYCH FPDQLLAQRA GLLKKIYRLP
160 170 180 190 200
FDLIEQFSVS AADTVVVNSN FTKNTFHQTF KYLSNDPDVI YPCVDLSTIE
210 220 230 240 250
IEDIDKKFFK TVFNEGDRFY LSINRFEKKK DVALAIKAFA LSEDQINDNV
260 270 280 290 300
KLVICGGYDE RVAENVEYLK ELQSLADEYE LSHTTIYYQE IKRVSDLESF
310 320 330 340 350
KTNNSKIIFL TSISSSLKEL LLERTEMLLY TPAYEHFGIV PLEAMKLGKP
360 370 380 390 400
VLAVNNGGPL ETIKSYVAGE NESSATGWLK PAVPIQWATA IDESRKILQN
410 420 430 440 450
GSVNFERNGP LRVKKYFSRE AMTQSFEENV EKVIWKEKKY YPWEIFGISF
460 470 480 490 500
SNFILHMAFI KILPNNPWPF LFMATFMVLY FKNYLWGIYW AFVFALSYPY

EEI
Length:503
Mass (Da):58,047
Last modified:October 1, 1996 - v2
Checksum:i1200E1C8EA023DD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8T → S in CAA61199 (PubMed:8400550).Curated1
Sequence conflicti237 – 238KA → NG in CAA61199 (PubMed:8400550).Curated2
Sequence conflicti302T → A in CAA61199 (PubMed:8400550).Curated1
Sequence conflicti457M → I in CAA61199 (PubMed:8400550).Curated1
Sequence conflicti470 – 503FLFMA…PYEEI → SYLWPLLWYYILRTTYGEFT GHLYSLSPTLMKKYNVYLNK QCIPPEEKIIYIGEEISKCK (PubMed:8400550).CuratedAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87947 Genomic DNA. Translation: CAA61199.1.
Z72587 Genomic DNA. Translation: CAA96768.1.
BK006941 Genomic DNA. Translation: DAA08037.1.
PIRiS64069.
RefSeqiNP_011450.1. NM_001180930.1.

Genome annotation databases

EnsemblFungiiYGL065C; YGL065C; YGL065C.
GeneIDi852815.
KEGGisce:YGL065C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87947 Genomic DNA. Translation: CAA61199.1.
Z72587 Genomic DNA. Translation: CAA96768.1.
BK006941 Genomic DNA. Translation: DAA08037.1.
PIRiS64069.
RefSeqiNP_011450.1. NM_001180930.1.

3D structure databases

ProteinModelPortaliP43636.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33182. 7 interactors.
DIPiDIP-5401N.
IntActiP43636. 4 interactors.
MINTiMINT-523155.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

PTM databases

iPTMnetiP43636.

Proteomic databases

MaxQBiP43636.
PRIDEiP43636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL065C; YGL065C; YGL065C.
GeneIDi852815.
KEGGisce:YGL065C.

Organism-specific databases

EuPathDBiFungiDB:YGL065C.
SGDiS000003033. ALG2.

Phylogenomic databases

GeneTreeiENSGT00550000075033.
HOGENOMiHOG000177048.
InParanoidiP43636.
KOiK03843.
OMAiKTHCFEE.
OrthoDBiEOG092C1IJD.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:YGL065C-MONOMER.
YEAST:YGL065C-MONOMER.
BRENDAi2.4.1.132. 984.
2.4.1.257. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP43636.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG2_YEAST
AccessioniPrimary (citable) accession number: P43636
Secondary accession number(s): D6VU76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.