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P43636

- ALG2_YEAST

UniProt

P43636 - ALG2_YEAST

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Protein
Alpha-1,3/1,6-mannosyltransferase ALG2
Gene
ALG2, YGL065C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.1 Publication

Catalytic activityi

GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.1 Publication
GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.1 Publication

Pathwayi

GO - Molecular functioni

  1. GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity Source: SGD
  2. glycolipid 6-alpha-mannosyltransferase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. mannosylation Source: GOC
  2. oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  3. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7186.
YEAST:YGL065C-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
Alternative name(s):
Asparagine-linked glycosylation protein 2
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene namesi
Name:ALG2
Ordered Locus Names:YGL065C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL065c.
SGDiS000003033. ALG2.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei64 – 8421Helical; Reviewed prediction
Add
BLAST
Transmembranei112 – 13221Helical; Reviewed prediction
Add
BLAST
Transmembranei443 – 46321Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: Reactome
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi335 – 3351E → A: Severly affects activity, especially the second mannosylation step. 1 Publication
Mutagenesisi343 – 3431E → A: No activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Alpha-1,3/1,6-mannosyltransferase ALG2
PRO_0000080271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi170 – 1701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi303 – 3031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi371 – 3711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi400 – 4001N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP43636.
PaxDbiP43636.
PeptideAtlasiP43636.

Expressioni

Gene expression databases

GenevestigatoriP43636.

Interactioni

Subunit structurei

Interacts with ALG1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ALG1P166612EBI-2459,EBI-2206309

Protein-protein interaction databases

BioGridi33182. 8 interactions.
DIPiDIP-5401N.
IntActiP43636. 4 interactions.
MINTiMINT-523155.
STRINGi4932.YGL065C.

Structurei

3D structure databases

ProteinModelPortaliP43636.
SMRiP43636. Positions 219-363.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00550000075033.
HOGENOMiHOG000177048.
KOiK03843.
OMAiGETGWLR.
OrthoDBiEOG7G4QQG.

Family and domain databases

InterProiIPR027054. ALG2.
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4_N.
[Graphical view]
PANTHERiPTHR12526:SF221. PTHR12526:SF221. 1 hit.
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43636-1 [UniParc]FASTAAdd to Basket

« Hide

MIEKDKRTIA FIHPDLGIGG AERLVVDAAL GLQQQGHSVI IYTSHCDKSH    50
CFEEVKNGQL KVEVYGDFLP TNFLGRFFIV FATIRQLYLV IQLILQKKVN 100
AYQLIIIDQL STCIPLLHIF SSATLMFYCH FPDQLLAQRA GLLKKIYRLP 150
FDLIEQFSVS AADTVVVNSN FTKNTFHQTF KYLSNDPDVI YPCVDLSTIE 200
IEDIDKKFFK TVFNEGDRFY LSINRFEKKK DVALAIKAFA LSEDQINDNV 250
KLVICGGYDE RVAENVEYLK ELQSLADEYE LSHTTIYYQE IKRVSDLESF 300
KTNNSKIIFL TSISSSLKEL LLERTEMLLY TPAYEHFGIV PLEAMKLGKP 350
VLAVNNGGPL ETIKSYVAGE NESSATGWLK PAVPIQWATA IDESRKILQN 400
GSVNFERNGP LRVKKYFSRE AMTQSFEENV EKVIWKEKKY YPWEIFGISF 450
SNFILHMAFI KILPNNPWPF LFMATFMVLY FKNYLWGIYW AFVFALSYPY 500
EEI 503
Length:503
Mass (Da):58,047
Last modified:October 1, 1996 - v2
Checksum:i1200E1C8EA023DD4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81T → S in CAA61199. 1 Publication
Sequence conflicti237 – 2382KA → NG in CAA61199. 1 Publication
Sequence conflicti302 – 3021T → A in CAA61199. 1 Publication
Sequence conflicti457 – 4571M → I in CAA61199. 1 Publication
Sequence conflicti470 – 50334FLFMA…PYEEI → SYLWPLLWYYILRTTYGEFT GHLYSLSPTLMKKYNVYLNK QCIPPEEKIIYIGEEISKCK1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87947 Genomic DNA. Translation: CAA61199.1.
Z72587 Genomic DNA. Translation: CAA96768.1.
BK006941 Genomic DNA. Translation: DAA08037.1.
PIRiS64069.
RefSeqiNP_011450.1. NM_001180930.1.

Genome annotation databases

EnsemblFungiiYGL065C; YGL065C; YGL065C.
GeneIDi852815.
KEGGisce:YGL065C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87947 Genomic DNA. Translation: CAA61199.1 .
Z72587 Genomic DNA. Translation: CAA96768.1 .
BK006941 Genomic DNA. Translation: DAA08037.1 .
PIRi S64069.
RefSeqi NP_011450.1. NM_001180930.1.

3D structure databases

ProteinModelPortali P43636.
SMRi P43636. Positions 219-363.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33182. 8 interactions.
DIPi DIP-5401N.
IntActi P43636. 4 interactions.
MINTi MINT-523155.
STRINGi 4932.YGL065C.

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBi P43636.
PaxDbi P43636.
PeptideAtlasi P43636.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL065C ; YGL065C ; YGL065C .
GeneIDi 852815.
KEGGi sce:YGL065C.

Organism-specific databases

CYGDi YGL065c.
SGDi S000003033. ALG2.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00550000075033.
HOGENOMi HOG000177048.
KOi K03843.
OMAi GETGWLR.
OrthoDBi EOG7G4QQG.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci MetaCyc:MONOMER-7186.
YEAST:YGL065C-MONOMER.

Miscellaneous databases

NextBioi 972351.
PROi P43636.

Gene expression databases

Genevestigatori P43636.

Family and domain databases

InterProi IPR027054. ALG2.
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4_N.
[Graphical view ]
PANTHERi PTHR12526:SF221. PTHR12526:SF221. 1 hit.
Pfami PF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biosynthesis of asparagine-linked oligosaccharides in Saccharomyces cerevisiae: the alg2 mutation."
    Jackson B.J., Kukuruzinska M.A., Robbins P.
    Glycobiology 3:357-364(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis."
    O'Reilly M.K., Zhang G., Imperiali B.
    Biochemistry 45:9593-9603(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, MUTAGENESIS OF GLU-335 AND GLU-343.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
    Gao X.-D., Nishikawa A., Dean N.
    Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALG1, SUBCELLULAR LOCATION.
  9. "A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis."
    Thiel C., Schwarz M., Peng J., Grzmil M., Hasilik M., Braulke T., Kohlschuetter A., von Figura K., Lehle L., Koerner C.
    J. Biol. Chem. 278:22498-22505(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiALG2_YEAST
AccessioniPrimary (citable) accession number: P43636
Secondary accession number(s): D6VU76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1840 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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