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P43636

- ALG2_YEAST

UniProt

P43636 - ALG2_YEAST

Protein

Alpha-1,3/1,6-mannosyltransferase ALG2

Gene

ALG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.1 Publication

    Catalytic activityi

    GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.1 Publication
    GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity Source: SGD
    2. glycolipid 6-alpha-mannosyltransferase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. mannosylation Source: GOC
    2. oligosaccharide-lipid intermediate biosynthetic process Source: SGD
    3. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-7186.
    YEAST:YGL065C-MONOMER.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
    Alternative name(s):
    Asparagine-linked glycosylation protein 2
    GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
    GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
    GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
    Gene namesi
    Name:ALG2
    Ordered Locus Names:YGL065C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL065c.
    SGDiS000003033. ALG2.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi335 – 3351E → A: Severly affects activity, especially the second mannosylation step. 1 Publication
    Mutagenesisi343 – 3431E → A: No activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 503503Alpha-1,3/1,6-mannosyltransferase ALG2PRO_0000080271Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP43636.
    PaxDbiP43636.
    PeptideAtlasiP43636.

    Expressioni

    Gene expression databases

    GenevestigatoriP43636.

    Interactioni

    Subunit structurei

    Interacts with ALG1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ALG1P166612EBI-2459,EBI-2206309

    Protein-protein interaction databases

    BioGridi33182. 8 interactions.
    DIPiDIP-5401N.
    IntActiP43636. 4 interactions.
    MINTiMINT-523155.
    STRINGi4932.YGL065C.

    Structurei

    3D structure databases

    ProteinModelPortaliP43636.
    SMRiP43636. Positions 219-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei443 – 46321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00550000075033.
    HOGENOMiHOG000177048.
    KOiK03843.
    OMAiGETGWLR.
    OrthoDBiEOG7G4QQG.

    Family and domain databases

    InterProiIPR027054. ALG2.
    IPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4_N.
    [Graphical view]
    PANTHERiPTHR12526:SF221. PTHR12526:SF221. 1 hit.
    PfamiPF13439. Glyco_transf_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43636-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEKDKRTIA FIHPDLGIGG AERLVVDAAL GLQQQGHSVI IYTSHCDKSH    50
    CFEEVKNGQL KVEVYGDFLP TNFLGRFFIV FATIRQLYLV IQLILQKKVN 100
    AYQLIIIDQL STCIPLLHIF SSATLMFYCH FPDQLLAQRA GLLKKIYRLP 150
    FDLIEQFSVS AADTVVVNSN FTKNTFHQTF KYLSNDPDVI YPCVDLSTIE 200
    IEDIDKKFFK TVFNEGDRFY LSINRFEKKK DVALAIKAFA LSEDQINDNV 250
    KLVICGGYDE RVAENVEYLK ELQSLADEYE LSHTTIYYQE IKRVSDLESF 300
    KTNNSKIIFL TSISSSLKEL LLERTEMLLY TPAYEHFGIV PLEAMKLGKP 350
    VLAVNNGGPL ETIKSYVAGE NESSATGWLK PAVPIQWATA IDESRKILQN 400
    GSVNFERNGP LRVKKYFSRE AMTQSFEENV EKVIWKEKKY YPWEIFGISF 450
    SNFILHMAFI KILPNNPWPF LFMATFMVLY FKNYLWGIYW AFVFALSYPY 500
    EEI 503
    Length:503
    Mass (Da):58,047
    Last modified:October 1, 1996 - v2
    Checksum:i1200E1C8EA023DD4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 81T → S in CAA61199. (PubMed:8400550)Curated
    Sequence conflicti237 – 2382KA → NG in CAA61199. (PubMed:8400550)Curated
    Sequence conflicti302 – 3021T → A in CAA61199. (PubMed:8400550)Curated
    Sequence conflicti457 – 4571M → I in CAA61199. (PubMed:8400550)Curated
    Sequence conflicti470 – 50334FLFMA…PYEEI → SYLWPLLWYYILRTTYGEFT GHLYSLSPTLMKKYNVYLNK QCIPPEEKIIYIGEEISKCK(PubMed:8400550)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87947 Genomic DNA. Translation: CAA61199.1.
    Z72587 Genomic DNA. Translation: CAA96768.1.
    BK006941 Genomic DNA. Translation: DAA08037.1.
    PIRiS64069.
    RefSeqiNP_011450.1. NM_001180930.1.

    Genome annotation databases

    EnsemblFungiiYGL065C; YGL065C; YGL065C.
    GeneIDi852815.
    KEGGisce:YGL065C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87947 Genomic DNA. Translation: CAA61199.1 .
    Z72587 Genomic DNA. Translation: CAA96768.1 .
    BK006941 Genomic DNA. Translation: DAA08037.1 .
    PIRi S64069.
    RefSeqi NP_011450.1. NM_001180930.1.

    3D structure databases

    ProteinModelPortali P43636.
    SMRi P43636. Positions 219-363.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33182. 8 interactions.
    DIPi DIP-5401N.
    IntActi P43636. 4 interactions.
    MINTi MINT-523155.
    STRINGi 4932.YGL065C.

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    Proteomic databases

    MaxQBi P43636.
    PaxDbi P43636.
    PeptideAtlasi P43636.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL065C ; YGL065C ; YGL065C .
    GeneIDi 852815.
    KEGGi sce:YGL065C.

    Organism-specific databases

    CYGDi YGL065c.
    SGDi S000003033. ALG2.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00550000075033.
    HOGENOMi HOG000177048.
    KOi K03843.
    OMAi GETGWLR.
    OrthoDBi EOG7G4QQG.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:MONOMER-7186.
    YEAST:YGL065C-MONOMER.

    Miscellaneous databases

    NextBioi 972351.
    PROi P43636.

    Gene expression databases

    Genevestigatori P43636.

    Family and domain databases

    InterProi IPR027054. ALG2.
    IPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4_N.
    [Graphical view ]
    PANTHERi PTHR12526:SF221. PTHR12526:SF221. 1 hit.
    Pfami PF13439. Glyco_transf_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis of asparagine-linked oligosaccharides in Saccharomyces cerevisiae: the alg2 mutation."
      Jackson B.J., Kukuruzinska M.A., Robbins P.
      Glycobiology 3:357-364(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis."
      O'Reilly M.K., Zhang G., Imperiali B.
      Biochemistry 45:9593-9603(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, MUTAGENESIS OF GLU-335 AND GLU-343.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
      Gao X.-D., Nishikawa A., Dean N.
      Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALG1, SUBCELLULAR LOCATION.
    9. "A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis."
      Thiel C., Schwarz M., Peng J., Grzmil M., Hasilik M., Braulke T., Kohlschuetter A., von Figura K., Lehle L., Koerner C.
      J. Biol. Chem. 278:22498-22505(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiALG2_YEAST
    AccessioniPrimary (citable) accession number: P43636
    Secondary accession number(s): D6VU76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1840 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3