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Protein

Citrate synthase 3, mitochondrial

Gene

CIT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity mitochondrial citrate and methylcitrate synthase with similar catalytic efficiency with both acetyl-CoA and propionyl-CoA.2 Publications

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.1 Publication

Kineticsi

  1. KM=1200 µM for acetyl-CoA1 Publication
  2. KM=520 µM for propionyl-CoA1 Publication

    Pathwayi: tricarboxylic acid cycle

    This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.Curated
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Citrate synthase, mitochondrial (CIT1), Citrate synthase 3, mitochondrial (CIT3), Citrate synthase, peroxisomal (CIT2)
    2. Aconitate hydratase, mitochondrial (ACO1)
    This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei315PROSITE-ProRule annotation1
    Active sitei361PROSITE-ProRule annotation1
    Active sitei419PROSITE-ProRule annotation1

    GO - Molecular functioni

    • 2-methylcitrate synthase activity Source: SGD
    • citrate (Si)-synthase activity Source: SGD

    GO - Biological processi

    • propionate catabolic process, 2-methylcitrate cycle Source: SGD
    • tricarboxylic acid cycle Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciMetaCyc:YPR001W-MONOMER.
    YEAST:YPR001W-MONOMER.
    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase 3, mitochondrial1 Publication (EC:2.3.3.11 Publication)
    Gene namesi
    Name:CIT31 Publication
    Ordered Locus Names:YPR001W
    ORF Names:YP9723.01
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPR001W.
    SGDiS000006205. CIT3.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrion Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Leads to an accumulation of acetate and of isobutanol.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 23MitochondrionSequence analysisAdd BLAST23
    ChainiPRO_000016998524 – 486Citrate synthase 3, mitochondrialAdd BLAST463

    Proteomic databases

    PRIDEiP43635.

    Interactioni

    Protein-protein interaction databases

    BioGridi36177. 26 interactors.
    DIPiDIP-3915N.
    MINTiMINT-562112.

    Structurei

    3D structure databases

    ProteinModelPortaliP43635.
    SMRiP43635.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi484 – 486Microbody targeting signalSequence analysis3

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    GeneTreeiENSGT00860000135371.
    HOGENOMiHOG000130831.
    InParanoidiP43635.
    KOiK01647.
    OMAiRFLIEMN.
    OrthoDBiEOG092C1UDR.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase.
    IPR019810. Citrate_synthase_AS.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43635-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVQRLLPGAH ICRRSFNSSA IIKSSALTLK EALENVIPKK RDAVKKLKAC
    60 70 80 90 100
    YGSTFVGPIT ISSVLGGMRG NQSMFWQGTS LDPEHGIKFQ GLTIEECQNR
    110 120 130 140 150
    LPNTGIDGDN FLPESMLWLL MTGGVPTFQQ AASFRKELAI RGRKLPHYTE
    160 170 180 190 200
    KVLSSLPKDM HPMTQLAIGL ASMNKGSLFA TNYQKGLIGK MEFWKDTLED
    210 220 230 240 250
    SLNLIASLPL LTGRIYSNIT NEGHPLGQYS EEVDWCTNIC SLLGMTNGTN
    260 270 280 290 300
    SSNTCNLTSQ QSLDFINLMR LYTGIHVDHE GGNVSAHTTH LVGSALSDPY
    310 320 330 340 350
    LSYSSGIMGL AGPLHGLAAQ EVVRFLIEMN SNISSIAREQ EIKDYLWKIL
    360 370 380 390 400
    NSNRVIPGYG HAVLRKPDPR FTAMLEFAQK RPIEFENDKN VLLMQKLAEI
    410 420 430 440 450
    APKVLLEHGK SKNPFPNVDS ASGILFYHYG IRELLFFTVI FGCSRAMGPL
    460 470 480
    TQLVWDRILG LPIERPKSLN LEGLEALTKA SNVNKL
    Length:486
    Mass (Da):53,811
    Last modified:November 1, 1995 - v1
    Checksum:iF299CA258B772125
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X88846 Genomic DNA. Translation: CAA61299.1.
    Z48951 Genomic DNA. Translation: CAA88779.1.
    Z71255 Genomic DNA. Translation: CAA95041.1.
    U31900 Genomic DNA. Translation: AAA97580.1.
    BK006949 Genomic DNA. Translation: DAA11428.1.
    PIRiS52814.
    RefSeqiNP_015325.1. NM_001184098.1.

    Genome annotation databases

    EnsemblFungiiYPR001W; YPR001W; YPR001W.
    GeneIDi856107.
    KEGGisce:YPR001W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X88846 Genomic DNA. Translation: CAA61299.1.
    Z48951 Genomic DNA. Translation: CAA88779.1.
    Z71255 Genomic DNA. Translation: CAA95041.1.
    U31900 Genomic DNA. Translation: AAA97580.1.
    BK006949 Genomic DNA. Translation: DAA11428.1.
    PIRiS52814.
    RefSeqiNP_015325.1. NM_001184098.1.

    3D structure databases

    ProteinModelPortaliP43635.
    SMRiP43635.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi36177. 26 interactors.
    DIPiDIP-3915N.
    MINTiMINT-562112.

    Proteomic databases

    PRIDEiP43635.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYPR001W; YPR001W; YPR001W.
    GeneIDi856107.
    KEGGisce:YPR001W.

    Organism-specific databases

    EuPathDBiFungiDB:YPR001W.
    SGDiS000006205. CIT3.

    Phylogenomic databases

    GeneTreeiENSGT00860000135371.
    HOGENOMiHOG000130831.
    InParanoidiP43635.
    KOiK01647.
    OMAiRFLIEMN.
    OrthoDBiEOG092C1UDR.

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER00717.
    BioCyciMetaCyc:YPR001W-MONOMER.
    YEAST:YPR001W-MONOMER.

    Miscellaneous databases

    PROiP43635.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase.
    IPR019810. Citrate_synthase_AS.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCISY3_YEAST
    AccessioniPrimary (citable) accession number: P43635
    Secondary accession number(s): D6W412
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: November 30, 2016
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.