Activatory protein CHA4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P43634 (CHA4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Activatory protein CHA4

Gene names

Name:	CHA4
Synonyms:	SIL2, SIL3
Ordered Locus Names:	YLR098C
ORF Names:	L8004.8

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates the CHA1 gene for L-serine dehydratase. Binds to the DNA sequence 5'-GVGGARAYRTRATTCCRC-3'. Ref.1
Subcellular location	Nucleus Probable.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid catabolic process Inferred from genetic interaction Ref.1. Source: SGD positive regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.1. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: GOC transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from physical interaction Ref.1. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 19111667 PubMed 19158363. Source: SGD sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.1. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 648

648

Activatory protein CHA4

PRO_0000114942

Regions

DNA binding

44 – 70

Zn(2)-C6 fungal-type

Compositional bias

32 – 35

Poly-Asn

Compositional bias

48 – 52

Poly-Arg

Compositional bias

105 – 109

Poly-Ser

Compositional bias

195 – 198

Poly-Gln

Compositional bias

302 – 306

Poly-Ser

Amino acid modifications

Modified residue

Phosphoserine Ref.6

Modified residue

164

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P43634 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CF3381B4C0356F82
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FASTA

648

74,393

        10         20         30         40         50         60 
MMLEPSPPPL TTTVTPSLPS SLKKSVTDND QNNNNVPRKR KLACQNCRRR RRKCNMEKPC 

        70         80         90        100        110        120 
SNCIKFRTEC VFTQQDLRNK RYSTTYVEAL QSQIRSLKEQ LQILSSSSST IASNALSSLK 

       130        140        150        160        170        180 
NNSDHGDAPN EKILKYGETA QSALPSSESN DENESDAFTK KMPSESPPPV GTNSIYPSNS 

       190        200        210        220        230        240 
LSIIKKKTDG STRYQQQQVS LKNLSRSPLI LRSLSLFFKW LYPGHYLFIH RETFLSAFFG 

       250        260        270        280        290        300 
DTNTKSYYCS EELVFAIAAL GSLISYKSET ELFQQSEVFY QRAKTIVLKK IFQLEDSSLA 

       310        320        330        340        350        360 
ESSSSSKLAI IQTLLCLAFY DIGSGENPMA WYLSGLAFRI AHEIGLHLNP EAWSNVYEDE 

       370        380        390        400        410        420 
LSIMDFEVRS RIYWGCYIAD HLIAILFGRS TSLRLSNSTV PETDELPEIE TGIEEYIYDP 

       430        440        450        460        470        480 
KVILSTANPL KKLIVLSRIT EIFASKIFSP NETLLQRSEY LAKFNLEVYN WRRDLPPELQ 

       490        500        510        520        530        540 
WTKRSLMEMT DFNPTIAYVW FHYYIVLISY NKPFIYEIKQ SRELVEGYVD ELYYLLKVWK 

       550        560        570        580        590        600 
NKFKTFEKAT IYMIYSAILA IQCMKSNLIK KDRKQDFLNF LSAPTLNYEL ARKFIENSED 

       610        620        630        640 
ALHNSETMDL LGTLSHGNDF ALEYNFDFTL LNEIDMLIGG NTNDGLSK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cha4p of Saccharomyces cerevisiae activates transcription via serine/threonine response elements." Holmberg S., Schjerling P. Genetics 144:467-478(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49975 Genomic DNA. Translation: CAA90276.1. U53876 Genomic DNA. Translation: AAB67542.1. Z73270 Genomic DNA. Translation: CAA97662.1. BK006945 Genomic DNA. Translation: DAA09414.1.
PIR	S59723.
RefSeq	NP_013199.1. NM_001181985.1.
3D structure databases
ProteinModelPortal	P43634.
SMR	P43634. Positions 42-73.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1798N.
IntAct	P43634. 9 interactions.
MINT	MINT-397314.
STRING	4932.YLR098C.
Proteomic databases
PaxDb	P43634.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR098C; YLR098C; YLR098C.
GeneID	850787.
KEGG	sce:YLR098C.
Organism-specific databases
CYGD	YLR098c.
SGD	S000004088. CHA4.
Phylogenomic databases
eggNOG	NOG75868.
GeneTree	ENSGT00530000067248.
HOGENOM	HOG000074724.
OMA	CLAFYDI.
OrthoDB	EOG4X9BRM.
Gene expression databases
Genevestigator	P43634.
GermOnline	YLR098C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.240.10. 1 hit.
InterPro	IPR007219. Transcription_factor_fun. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Pfam	PF04082. Fungal_trans. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00906. Fungal_trans. 1 hit. SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966987.

Entry information

Entry name

CHA4_YEAST

Accession

Primary (citable) accession number: P43634
Secondary accession number(s): D6VY98

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents