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P43634 (CHA4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Activatory protein CHA4
Gene names
Name:CHA4
Synonyms:SIL2, SIL3
Ordered Locus Names:YLR098C
ORF Names:L8004.8
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates the CHA1 gene for L-serine dehydratase. Binds to the DNA sequence 5'-GVGGARAYRTRATTCCRC-3'. Ref.1

Subcellular location

Nucleus Probable.

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648Activatory protein CHA4
PRO_0000114942

Regions

DNA binding44 – 7027Zn(2)-C6 fungal-type
Compositional bias32 – 354Poly-Asn
Compositional bias48 – 525Poly-Arg
Compositional bias105 – 1095Poly-Ser
Compositional bias195 – 1984Poly-Gln
Compositional bias302 – 3065Poly-Ser

Amino acid modifications

Modified residue1641Phosphoserine Ref.5 Ref.7
Modified residue1661Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P43634 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CF3381B4C0356F82

FASTA64874,393
        10         20         30         40         50         60 
MMLEPSPPPL TTTVTPSLPS SLKKSVTDND QNNNNVPRKR KLACQNCRRR RRKCNMEKPC 

        70         80         90        100        110        120 
SNCIKFRTEC VFTQQDLRNK RYSTTYVEAL QSQIRSLKEQ LQILSSSSST IASNALSSLK 

       130        140        150        160        170        180 
NNSDHGDAPN EKILKYGETA QSALPSSESN DENESDAFTK KMPSESPPPV GTNSIYPSNS 

       190        200        210        220        230        240 
LSIIKKKTDG STRYQQQQVS LKNLSRSPLI LRSLSLFFKW LYPGHYLFIH RETFLSAFFG 

       250        260        270        280        290        300 
DTNTKSYYCS EELVFAIAAL GSLISYKSET ELFQQSEVFY QRAKTIVLKK IFQLEDSSLA 

       310        320        330        340        350        360 
ESSSSSKLAI IQTLLCLAFY DIGSGENPMA WYLSGLAFRI AHEIGLHLNP EAWSNVYEDE 

       370        380        390        400        410        420 
LSIMDFEVRS RIYWGCYIAD HLIAILFGRS TSLRLSNSTV PETDELPEIE TGIEEYIYDP 

       430        440        450        460        470        480 
KVILSTANPL KKLIVLSRIT EIFASKIFSP NETLLQRSEY LAKFNLEVYN WRRDLPPELQ 

       490        500        510        520        530        540 
WTKRSLMEMT DFNPTIAYVW FHYYIVLISY NKPFIYEIKQ SRELVEGYVD ELYYLLKVWK 

       550        560        570        580        590        600 
NKFKTFEKAT IYMIYSAILA IQCMKSNLIK KDRKQDFLNF LSAPTLNYEL ARKFIENSED 

       610        620        630        640 
ALHNSETMDL LGTLSHGNDF ALEYNFDFTL LNEIDMLIGG NTNDGLSK 

« Hide

References

« Hide 'large scale' references
[1]"Cha4p of Saccharomyces cerevisiae activates transcription via serine/threonine response elements."
Holmberg S., Schjerling P.
Genetics 144:467-478(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164 AND SER-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49975 Genomic DNA. Translation: CAA90276.1.
U53876 Genomic DNA. Translation: AAB67542.1.
Z73270 Genomic DNA. Translation: CAA97662.1.
BK006945 Genomic DNA. Translation: DAA09414.1.
PIRS59723.
RefSeqNP_013199.1. NM_001181985.1.

3D structure databases

ProteinModelPortalP43634.
SMRP43634. Positions 42-73.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31371. 75 interactions.
DIPDIP-1798N.
IntActP43634. 10 interactions.
MINTMINT-397314.
STRING4932.YLR098C.

Proteomic databases

MaxQBP43634.
PaxDbP43634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR098C; YLR098C; YLR098C.
GeneID850787.
KEGGsce:YLR098C.

Organism-specific databases

CYGDYLR098c.
SGDS000004088. CHA4.

Phylogenomic databases

eggNOGNOG75868.
GeneTreeENSGT00530000067248.
HOGENOMHOG000074724.
OMAMAWYLSG.
OrthoDBEOG7Q5HPH.

Enzyme and pathway databases

BioCycYEAST:G3O-32248-MONOMER.

Gene expression databases

GenevestigatorP43634.

Family and domain databases

Gene3D4.10.240.10. 1 hit.
InterProIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMSSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966987.

Entry information

Entry nameCHA4_YEAST
AccessionPrimary (citable) accession number: P43634
Secondary accession number(s): D6VY98
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families