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P43634

- CHA4_YEAST

UniProt

P43634 - CHA4_YEAST

Protein

Activatory protein CHA4

Gene

CHA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Activates the CHA1 gene for L-serine dehydratase. Binds to the DNA sequence 5'-GVGGARAYRTRATTCCRC-3'.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi44 – 7027Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: SGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular amino acid catabolic process Source: SGD
    2. positive regulation of transcription, DNA-templated Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32248-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Activatory protein CHA4
    Gene namesi
    Name:CHA4
    Synonyms:SIL2, SIL3
    Ordered Locus Names:YLR098C
    ORF Names:L8004.8
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR098c.
    SGDiS000004088. CHA4.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 648648Activatory protein CHA4PRO_0000114942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641Phosphoserine2 Publications
    Modified residuei166 – 1661Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43634.
    PaxDbiP43634.

    Expressioni

    Gene expression databases

    GenevestigatoriP43634.

    Interactioni

    Protein-protein interaction databases

    BioGridi31371. 75 interactions.
    DIPiDIP-1798N.
    IntActiP43634. 10 interactions.
    MINTiMINT-397314.
    STRINGi4932.YLR098C.

    Structurei

    3D structure databases

    ProteinModelPortaliP43634.
    SMRiP43634. Positions 42-73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi32 – 354Poly-Asn
    Compositional biasi48 – 525Poly-Arg
    Compositional biasi105 – 1095Poly-Ser
    Compositional biasi195 – 1984Poly-Gln
    Compositional biasi302 – 3065Poly-Ser

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG75868.
    GeneTreeiENSGT00530000067248.
    HOGENOMiHOG000074724.
    OMAiMAWYLSG.
    OrthoDBiEOG7Q5HPH.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    PfamiPF04082. Fungal_trans. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view]
    SMARTiSM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43634-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMLEPSPPPL TTTVTPSLPS SLKKSVTDND QNNNNVPRKR KLACQNCRRR    50
    RRKCNMEKPC SNCIKFRTEC VFTQQDLRNK RYSTTYVEAL QSQIRSLKEQ 100
    LQILSSSSST IASNALSSLK NNSDHGDAPN EKILKYGETA QSALPSSESN 150
    DENESDAFTK KMPSESPPPV GTNSIYPSNS LSIIKKKTDG STRYQQQQVS 200
    LKNLSRSPLI LRSLSLFFKW LYPGHYLFIH RETFLSAFFG DTNTKSYYCS 250
    EELVFAIAAL GSLISYKSET ELFQQSEVFY QRAKTIVLKK IFQLEDSSLA 300
    ESSSSSKLAI IQTLLCLAFY DIGSGENPMA WYLSGLAFRI AHEIGLHLNP 350
    EAWSNVYEDE LSIMDFEVRS RIYWGCYIAD HLIAILFGRS TSLRLSNSTV 400
    PETDELPEIE TGIEEYIYDP KVILSTANPL KKLIVLSRIT EIFASKIFSP 450
    NETLLQRSEY LAKFNLEVYN WRRDLPPELQ WTKRSLMEMT DFNPTIAYVW 500
    FHYYIVLISY NKPFIYEIKQ SRELVEGYVD ELYYLLKVWK NKFKTFEKAT 550
    IYMIYSAILA IQCMKSNLIK KDRKQDFLNF LSAPTLNYEL ARKFIENSED 600
    ALHNSETMDL LGTLSHGNDF ALEYNFDFTL LNEIDMLIGG NTNDGLSK 648
    Length:648
    Mass (Da):74,393
    Last modified:November 1, 1995 - v1
    Checksum:iCF3381B4C0356F82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49975 Genomic DNA. Translation: CAA90276.1.
    U53876 Genomic DNA. Translation: AAB67542.1.
    Z73270 Genomic DNA. Translation: CAA97662.1.
    BK006945 Genomic DNA. Translation: DAA09414.1.
    PIRiS59723.
    RefSeqiNP_013199.1. NM_001181985.1.

    Genome annotation databases

    EnsemblFungiiYLR098C; YLR098C; YLR098C.
    GeneIDi850787.
    KEGGisce:YLR098C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49975 Genomic DNA. Translation: CAA90276.1 .
    U53876 Genomic DNA. Translation: AAB67542.1 .
    Z73270 Genomic DNA. Translation: CAA97662.1 .
    BK006945 Genomic DNA. Translation: DAA09414.1 .
    PIRi S59723.
    RefSeqi NP_013199.1. NM_001181985.1.

    3D structure databases

    ProteinModelPortali P43634.
    SMRi P43634. Positions 42-73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31371. 75 interactions.
    DIPi DIP-1798N.
    IntActi P43634. 10 interactions.
    MINTi MINT-397314.
    STRINGi 4932.YLR098C.

    Proteomic databases

    MaxQBi P43634.
    PaxDbi P43634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR098C ; YLR098C ; YLR098C .
    GeneIDi 850787.
    KEGGi sce:YLR098C.

    Organism-specific databases

    CYGDi YLR098c.
    SGDi S000004088. CHA4.

    Phylogenomic databases

    eggNOGi NOG75868.
    GeneTreei ENSGT00530000067248.
    HOGENOMi HOG000074724.
    OMAi MAWYLSG.
    OrthoDBi EOG7Q5HPH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32248-MONOMER.

    Miscellaneous databases

    NextBioi 966987.

    Gene expression databases

    Genevestigatori P43634.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    Pfami PF04082. Fungal_trans. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view ]
    SMARTi SM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cha4p of Saccharomyces cerevisiae activates transcription via serine/threonine response elements."
      Holmberg S., Schjerling P.
      Genetics 144:467-478(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164 AND SER-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCHA4_YEAST
    AccessioniPrimary (citable) accession number: P43634
    Secondary accession number(s): D6VY98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 396 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3