P43632 (KI2S4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Killer cell immunoglobulin-like receptor 2DS4

Alternative name(s):
CD158 antigen-like family member I
MHC class I NK cell receptor
Natural killer-associated transcript 8
Short name=NKAT-8
P58 natural killer cell receptor clones CL-39/CL-17
Short name=p58 NK receptor CL-39/CL-17
CD_antigen=CD158i

Gene names

Name:	KIR2DS4
Synonyms:	CD158I, KKA3, NKAT8

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells. Ref.5
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Sequence caution	The sequence AAC50336.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

By similarity

Chain

22 – 304

283

Killer cell immunoglobulin-like receptor 2DS4

PRO_0000015085

Regions

Topological domain

22 – 245

224

Extracellular Potential

Transmembrane

246 – 265

20

Helical; Potential

Topological domain

266 – 304

39

Cytoplasmic Potential

Domain

42 – 107

66

Ig-like C2-type 1

Domain

142 – 205

64

Ig-like C2-type 2

Amino acid modifications

Glycosylation

67

1

N-linked (GlcNAc...) Potential

Glycosylation

84

1

N-linked (GlcNAc...) Potential

Glycosylation

144

1

N-linked (GlcNAc...) Potential

Glycosylation

178

1

N-linked (GlcNAc...) Potential

Glycosylation

211

1

N-linked (GlcNAc...) Potential

Disulfide bond

49 ↔ 100

Ref.5

Disulfide bond

149 ↔ 198

Ref.5

Natural variations

Natural variant

65

1

K → M.
Corresponds to variant rs1130480 [ dbSNP | Ensembl ].

VAR_049980

Natural variant

68

1

N → D.
Corresponds to variant rs1130481 [ dbSNP | Ensembl ].

VAR_049981

Natural variant

71

1

H → R.
Corresponds to variant rs1130482 [ dbSNP | Ensembl ].

VAR_049982

Natural variant

89

1

P → R.
Corresponds to variant rs1130487 [ dbSNP | Ensembl ].

VAR_049983

Natural variant

92

1

P → H.
Corresponds to variant rs1143508 [ dbSNP | Ensembl ].

VAR_049984

Natural variant

93

1

V → D.
Corresponds to variant rs1130491 [ dbSNP | Ensembl ].

VAR_049985

Natural variant

103

1

S → C.
Corresponds to variant rs10406301 [ dbSNP | Ensembl ].

VAR_049986

Natural variant

178

1

N → H.
Corresponds to variant rs4806591 [ dbSNP | Ensembl ].

VAR_059423

Natural variant

205

1

A → S.
Corresponds to variant rs1049290 [ dbSNP | Ensembl ].

VAR_059424

Natural variant

254

1

K → N.
Corresponds to variant rs1063326 [ dbSNP | Ensembl ].

VAR_059425

Secondary structure

1

.

.

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304

Helix Strand Turn

Details...

Beta strand

30 – 34

5

Beta strand

38 – 40

3

Beta strand

44 – 53

10

Beta strand

58 – 62

5

Beta strand

68 – 72

5

Beta strand

81 – 87

7

Beta strand

98 – 101

4

Beta strand

121 – 123

3

Beta strand

130 – 134

5

Beta strand

146 – 153

8

Beta strand

156 – 162

7

Beta strand

169 – 172

4

Beta strand

181 – 186

6

Beta strand

196 – 199

4

Sequences

	Sequence		Length	Mass (Da)
	P43632 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: CEFDAFF3FD679A3F	FASTA	304	33,583
	10 20 30 40 50 60 MSLMVIIMAC VGFFLLQGAW PQEGVHRKPS FLALPGHLVK SEETVILQCW SDVMFEHFLL 70 80 90 100 110 120 HREGKFNNTL HLIGEHHDGV SKANFSIGPM MPVLAGTYRC YGSVPHSPYQ LSAPSDPLDM 130 140 150 160 170 180 VIIGLYEKPS LSAQPGPTVQ AGENVTLSCS SRSSYDMYHL SREGEAHERR LPAVRSINGT 190 200 210 220 230 240 FQADFPLGPA THGGTYRCFG SFRDAPYEWS NSSDPLLVSV TGNPSNSWPS PTEPSSKTGN 250 260 270 280 290 300 PRHLHVLIGT SVVKIPFTIL LFFLLHRWCS DKKNAAVMDQ EPAGNRTVNS EDSDEQDHQE VSYA

References

[1]	"Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains." Wagtmann N., Biassoni R., Cantoni C., Verdiani S., Malnati M.S., Vitale M., Bottino C., Moretta L., Moretta A., Long E.O. Immunity 2:439-449(1995) [PubMed: 7749980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Natural killer cell.
[2]	"Alternatively spliced forms of human killer inhibitory receptors." Doehring C., Samaridis J., Colonna M. Immunogenetics 44:227-230(1996) [PubMed: 8662091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A novel surface molecule homologous to the p58/p50 family of receptors is selectively expressed on a subset of human natural killer cells and induces both triggering of cell functions and proliferation." Bottino C., Sivori S., Vitale M., Cantoni C., Falco M., Pende D., Morelli L., Augugliaro R., Semenzato G., Biassoni R., Moretta L., Moretta A. Eur. J. Immunol. 26:1816-1824(1996) [PubMed: 8765026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoid tissue.
[4]	Chan H.W., Salter R.D. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"KIR2DS4 is a product of gene conversion with KIR3DL2 that introduced specificity for HLA-A*11 while diminishing avidity for HLA-C." Graef T., Moesta A.K., Norman P.J., Abi-Rached L., Vago L., Older Aguilar A.M., Gleimer M., Hammond J.A., Guethlein L.A., Bushnell D.A., Robinson P.J., Parham P. J. Exp. Med. 206:2557-2572(2009) [PubMed: 19858347] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 27-221, FUNCTION, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U24077 mRNA. Translation: AAC50336.1. Different initiation.
L76671 mRNA. Translation: AAB36599.1.
X94609 mRNA. Translation: CAA64317.1.
AF002255 mRNA. Translation: AAB61281.1.

IPI

IPI00019905.

RefSeq

NP_036446.3. NM_012314.3.

UniGene

Hs.654608.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3H8N	X-ray	2.50	A	27-221	[»]

ProteinModelPortal

P43632.

SMR

P43632. Positions 27-221.

ModBase

Search...

Protein-protein interaction databases

STRING

P43632.

Polymorphism databases

DMDM

2828511.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3809.

KEGG

hsa:3809.

Organism-specific databases

CTD

3809.

GeneCards

GC19P055344.

HGNC

HGNC:6336. KIR2DS4.

MIM

604955. gene.

neXtProt

NX_P43632.

PharmGKB

PA30121.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG074353.

InParanoid

P43632.

Gene expression databases

CleanEx

HS_KIR2DS4.

Genevestigator

P43632.

GermOnline

ENSG00000125498. Homo sapiens.

Family and domain databases

InterPro

IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 2 hits.

KO

K07982.

Pfam

PF00047. ig. 2 hits.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

14969.

SOURCE

Search...

Entry information

Entry name

KI2S4_HUMAN

Accession

Primary (citable) accession number: P43632

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.