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P43628 (KI2L3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Killer cell immunoglobulin-like receptor 2DL3
Alternative name(s):
CD158 antigen-like family member B2
KIR-023GB
Killer inhibitory receptor cl 2-3
MHC class I NK cell receptor
NKAT2a
NKAT2b
Natural killer-associated transcript 2
Short name=NKAT-2
p58 natural killer cell receptor clone CL-6
Short name=p58 NK receptor CL-6
p58.2 MHC class-I-specific NK receptor
CD_antigen=CD158b2
Gene names
Name:KIR2DL3
Synonyms:CD158B2, KIRCL23, NKAT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). Inhibits the activity of NK cells thus preventing cell lysis.

Subunit structure

Interacts with ARRB2. Ref.9

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the immunoglobulin superfamily.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PTPN11Q061244EBI-8632435,EBI-297779
PTPN6P2935013EBI-8632435,EBI-78260

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P43628-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P43628-2)

Also known as: NKAT2a-delta-Ig2;

The sequence of this isoform differs from the canonical sequence as follows:
     117-214: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 341320Killer cell immunoglobulin-like receptor 2DL3
PRO_0000015080

Regions

Topological domain22 – 245224Extracellular Potential
Transmembrane246 – 26520Helical; Potential
Topological domain266 – 34176Cytoplasmic Potential
Domain42 – 10766Ig-like C2-type 1
Domain142 – 20564Ig-like C2-type 2

Amino acid modifications

Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2111N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 100 By similarity
Disulfide bond149 ↔ 198 By similarity

Natural variations

Alternative sequence117 – 21498Missing in isoform 2.
VSP_007814
Natural variant91V → A. Ref.4 Ref.5
Corresponds to variant rs3810343 [ dbSNP | Ensembl ].
VAR_010313
Natural variant321L → R. Ref.4 Ref.5
VAR_010314
Natural variant341H → Q. Ref.8
VAR_015967
Natural variant371P → R.
Corresponds to variant rs613240 [ dbSNP | Ensembl ].
VAR_049978
Natural variant561Q → E. Ref.4 Ref.5
Corresponds to variant rs35719984 [ dbSNP | Ensembl ].
VAR_010315
Natural variant661F → Y.
Corresponds to variant rs673568 [ dbSNP | Ensembl ].
VAR_049979
Natural variant711H → R. Ref.4 Ref.5
Corresponds to variant rs138897134 [ dbSNP | Ensembl ].
VAR_010316
Natural variant2291P → L. Ref.3 Ref.6 Ref.7
Corresponds to variant rs35861855 [ dbSNP | Ensembl ].
VAR_015968
Natural variant2421R → I. Ref.5
VAR_015969
Natural variant3041A → T. Ref.3
Corresponds to variant rs4020187 [ dbSNP | Ensembl ].
VAR_015970
Natural variant3181R → H. Ref.3 Ref.4 Ref.5 Ref.6
Corresponds to variant rs1049267 [ dbSNP | Ensembl ].
VAR_010317

Secondary structure

............................... 341
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: C21EAFF80EA9AAB2

FASTA34137,886
        10         20         30         40         50         60 
MSLMVVSMVC VGFFLLQGAW PHEGVHRKPS LLAHPGPLVK SEETVILQCW SDVRFQHFLL 

        70         80         90        100        110        120 
HREGKFKDTL HLIGEHHDGV SKANFSIGPM MQDLAGTYRC YGSVTHSPYQ LSAPSDPLDI 

       130        140        150        160        170        180 
VITGLYEKPS LSAQPGPTVL AGESVTLSCS SRSSYDMYHL SREGEAHERR FSAGPKVNGT 

       190        200        210        220        230        240 
FQADFPLGPA THGGTYRCFG SFRDSPYEWS NSSDPLLVSV TGNPSNSWPS PTEPSSETGN 

       250        260        270        280        290        300 
PRHLHVLIGT SVVIILFILL LFFLLHRWCC NKKNAVVMDQ EPAGNRTVNR EDSDEQDPQE 

       310        320        330        340 
VTYAQLNHCV FTQRKITRPS QRPKTPPTDI IVYTELPNAE P 

« Hide

Isoform 2 (NKAT2a-delta-Ig2) [UniParc].

Checksum: 86E3E532F7335609
Show »

FASTA24327,269

References

« Hide 'large scale' references
[1]"Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells."
Colonna M., Samaridis J.
Science 268:405-408(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Natural killer cell.
[2]"Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains."
Wagtmann N., Biassoni R., Cantoni C., Verdiani S., Malnati M.S., Vitale M., Bottino C., Moretta L., Moretta A., Long E.O.
Immunity 2:439-449(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Natural killer cell.
[3]"Alternatively spliced forms of human killer inhibitory receptors."
Doehring C., Samaridis J., Colonna M.
Immunogenetics 44:227-230(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS LEU-229; THR-304 AND HIS-318.
[4]"Human diversity in killer cell inhibitory receptor genes."
Uhrberg M., Valiante N.M., Shum B.P., Shilling H.G., Lienert-Weidenbach K., Corliss B., Tyan D., Lanier L.L., Parham P.
Immunity 7:753-763(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), VARIANTS ALA-9; ARG-32; GLU-56; ARG-71 AND HIS-318.
[5]"Polymorphism and domain variability of human killer cell inhibitory receptors."
Selvakumar A., Steffens U., Dupont B.
Immunol. Rev. 155:183-196(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ALA-9; ARG-32; GLU-56; ARG-71; ILE-242 AND HIS-318.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS LEU-229 AND HIS-318.
Tissue: Blood.
[7]"Genomic organization of a human killer cell inhibitory receptor gene."
Wilson M.J., Torkar M., Trowsdale J.
Tissue Antigens 49:574-579(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298 (ISOFORM 1), VARIANT LEU-229.
[8]"Genomic organization of novel genes encoding human killer inhibitory receptors."
Chae J.H., Kim Y.M., Ahn J.M., Kim C.G., Kim J., Kim S.J., Park J.H.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-341, VARIANT GLN-34.
Tissue: Placenta.
[9]"An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells."
Yu M.-C., Su L.-L., Zou L., Liu Y., Wu N., Kong L., Zhuang Z.-H., Sun L., Liu H.P., Hu J.-H., Li D., Strominger J.L., Zang J.-W., Pei G., Ge B.-X.
Nat. Immunol. 9:898-907(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARRB2.
[10]"Crystal structure of the human p58 killer cell inhibitory receptor (KIR2DL3) specific for HLA-Cw3-related MHC class I."
Maenaka K., Juji T., Stuart D.I., Jones E.Y.
Structure 7:391-398(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 22-245.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L41268 mRNA. Translation: AAA69869.1.
U24074 mRNA. Translation: AAC50333.1.
L76662 mRNA. Translation: AAB36590.1.
L76663 mRNA. Translation: AAB36591.1.
AF022048 mRNA. Translation: AAB95321.1.
U73395 mRNA. Translation: AAC51147.1.
BC032422 mRNA. Translation: AAH32422.1.
BC050730 mRNA. Translation: AAH50730.1.
AH006692 Genomic DNA. Translation: AAC51651.1.
AH007572 Genomic DNA. Translation: AAD24477.1.
PIRI61725.
RefSeqNP_056952.2. NM_015868.2.
UniGeneHs.654608.
Hs.731378.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B6UX-ray3.00A22-245[»]
ProteinModelPortalP43628.
SMRP43628. Positions 26-224.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110005. 2 interactions.
IntActP43628. 3 interactions.
MINTMINT-1523646.

Polymorphism databases

DMDM1171727.

Proteomic databases

PRIDEP43628.

Protocols and materials databases

DNASU3804.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000342376; ENSP00000342215; ENSG00000243772. [P43628-1]
ENST00000571420; ENSP00000461891; ENSG00000261912.
ENST00000576013; ENSP00000461171; ENSG00000263027. [P43628-1]
ENST00000603813; ENSP00000474627; ENSG00000270787. [P43628-1]
GeneID3804.
KEGGhsa:3804.
UCSCuc002qgx.3. human. [P43628-1]

Organism-specific databases

CTD3804.
GeneCardsGC19P055273.
HGNCHGNC:6331. KIR2DL3.
MIM604938. gene.
neXtProtNX_P43628.
PharmGKBPA30116.
GenAtlasSearch...

Phylogenomic databases

HOVERGENHBG074353.
InParanoidP43628.
KOK07981.
OMAEEAHESW.
PhylomeDBP43628.
TreeFamTF352669.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

GenevestigatorP43628.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP43628.
NextBio14943.
PROP43628.
SOURCESearch...

Entry information

Entry nameKI2L3_HUMAN
AccessionPrimary (citable) accession number: P43628
Secondary accession number(s): O43472 expand/collapse secondary AC list , P78402, Q14944, Q14945, Q9UM51, Q9UQ70
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries