P43628 (KI2L3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Killer cell immunoglobulin-like receptor 2DL3

Alternative name(s):
CD158 antigen-like family member B2
KIR-023GB
Killer inhibitory receptor cl 2-3
MHC class I NK cell receptor
NKAT2a
NKAT2b
Natural killer-associated transcript 2
Short name=NKAT-2
p58 natural killer cell receptor clone CL-6
Short name=p58 NK receptor CL-6
p58.2 MHC class-I-specific NK receptor
CD_antigen=CD158b2

Gene names

Name:	KIR2DL3
Synonyms:	CD158B2, KIRCL23, NKAT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). Inhibits the activity of NK cells thus preventing cell lysis.
Subunit structure	Interacts with ARRB2. Ref.9
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	immune response Traceable author statement Ref.2. Source: ProtInc regulation of immune response Traceable author statement. Source: Reactome
Cellular_component	integral to plasma membrane Traceable author statement Ref.5. Source: ProtInc
Molecular_function	antigen binding Traceable author statement Ref.5. Source: ProtInc receptor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P43628-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P43628-2)

Also known as: NKAT2a-delta-Ig2;

The sequence of this isoform differs from the canonical sequence as follows:
     117-214: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

By similarity

Chain

22 – 341

320

Killer cell immunoglobulin-like receptor 2DL3

PRO_0000015080

Regions

Topological domain

22 – 245

224

Extracellular Potential

Transmembrane

246 – 265

20

Helical; Potential

Topological domain

266 – 341

76

Cytoplasmic Potential

Domain

42 – 107

66

Ig-like C2-type 1

Domain

142 – 205

64

Ig-like C2-type 2

Amino acid modifications

Glycosylation

84

1

N-linked (GlcNAc...) Potential

Glycosylation

178

1

N-linked (GlcNAc...) Potential

Glycosylation

211

1

N-linked (GlcNAc...) Potential

Disulfide bond

49 ↔ 100

By similarity

Disulfide bond

149 ↔ 198

By similarity

Natural variations

Alternative sequence

117 – 214

98

Missing in isoform 2.

VSP_007814

Natural variant

9

1

V → A. Ref.4 Ref.5
Corresponds to variant rs3810343 [ dbSNP | Ensembl ].

VAR_010313

Natural variant

32

1

L → R. Ref.4 Ref.5

VAR_010314

Natural variant

34

1

H → Q. Ref.8

VAR_015967

Natural variant

37

1

P → R.
Corresponds to variant rs613240 [ dbSNP | Ensembl ].

VAR_049978

Natural variant

56

1

Q → E. Ref.4 Ref.5
Corresponds to variant rs35719984 [ dbSNP | Ensembl ].

VAR_010315

Natural variant

66

1

F → Y.
Corresponds to variant rs673568 [ dbSNP | Ensembl ].

VAR_049979

Natural variant

71

1

H → R. Ref.4 Ref.5

VAR_010316

Natural variant

229

1

P → L. Ref.3 Ref.6 Ref.7
Corresponds to variant rs35861855 [ dbSNP | Ensembl ].

VAR_015968

Natural variant

242

1

R → I. Ref.5

VAR_015969

Natural variant

304

1

A → T. Ref.3
Corresponds to variant rs4020187 [ dbSNP | Ensembl ].

VAR_015970

Natural variant

318

1

R → H. Ref.3 Ref.4 Ref.5 Ref.6
Corresponds to variant rs1049267 [ dbSNP | Ensembl ].

VAR_010317

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

341

Helix Strand Turn

Details...

Beta strand

37 – 42

6

Beta strand

45 – 53

9

Beta strand

56 – 64

9

Beta strand

68 – 73

6

Beta strand

81 – 87

7

Turn

92 – 94

3

Beta strand

96 – 103

8

Beta strand

118 – 123

6

Beta strand

130 – 132

3

Beta strand

144 – 150

7

Beta strand

156 – 162

7

Beta strand

177 – 179

3

Beta strand

182 – 189

8

Beta strand

194 – 201

8

Beta strand

216 – 218

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C21EAFF80EA9AAB2	FASTA	341	37,886
	10 20 30 40 50 60 MSLMVVSMVC VGFFLLQGAW PHEGVHRKPS LLAHPGPLVK SEETVILQCW SDVRFQHFLL 70 80 90 100 110 120 HREGKFKDTL HLIGEHHDGV SKANFSIGPM MQDLAGTYRC YGSVTHSPYQ LSAPSDPLDI 130 140 150 160 170 180 VITGLYEKPS LSAQPGPTVL AGESVTLSCS SRSSYDMYHL SREGEAHERR FSAGPKVNGT 190 200 210 220 230 240 FQADFPLGPA THGGTYRCFG SFRDSPYEWS NSSDPLLVSV TGNPSNSWPS PTEPSSETGN 250 260 270 280 290 300 PRHLHVLIGT SVVIILFILL LFFLLHRWCC NKKNAVVMDQ EPAGNRTVNR EDSDEQDPQE 310 320 330 340 VTYAQLNHCV FTQRKITRPS QRPKTPPTDI IVYTELPNAE P
	Isoform 2 (NKAT2a-delta-Ig2) [UniParc]. Checksum: 86E3E532F7335609	FASTA	243	27,269
	10 20 30 40 50 60 MSLMVVSMVC VGFFLLQGAW PHEGVHRKPS LLAHPGPLVK SEETVILQCW SDVRFQHFLL 70 80 90 100 110 120 HREGKFKDTL HLIGEHHDGV SKANFSIGPM MQDLAGTYRC YGSVTHSPYQ LSAPSDPLLV 130 140 150 160 170 180 SVTGNPSNSW PSPTEPSSET GNPRHLHVLI GTSVVIILFI LLLFFLLHRW CCNKKNAVVM 190 200 210 220 230 240 DQEPAGNRTV NREDSDEQDP QEVTYAQLNH CVFTQRKITR PSQRPKTPPT DIIVYTELPN AEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells." Colonna M., Samaridis J. Science 268:405-408(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Natural killer cell.
[2]	"Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains." Wagtmann N., Biassoni R., Cantoni C., Verdiani S., Malnati M.S., Vitale M., Bottino C., Moretta L., Moretta A., Long E.O. Immunity 2:439-449(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Natural killer cell.
[3]	"Alternatively spliced forms of human killer inhibitory receptors." Doehring C., Samaridis J., Colonna M. Immunogenetics 44:227-230(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS LEU-229; THR-304 AND HIS-318.
[4]	"Human diversity in killer cell inhibitory receptor genes." Uhrberg M., Valiante N.M., Shum B.P., Shilling H.G., Lienert-Weidenbach K., Corliss B., Tyan D., Lanier L.L., Parham P. Immunity 7:753-763(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), VARIANTS ALA-9; ARG-32; GLU-56; ARG-71 AND HIS-318.
[5]	"Polymorphism and domain variability of human killer cell inhibitory receptors." Selvakumar A., Steffens U., Dupont B. Immunol. Rev. 155:183-196(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ALA-9; ARG-32; GLU-56; ARG-71; ILE-242 AND HIS-318.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS LEU-229 AND HIS-318. Tissue: Blood.
[7]	"Genomic organization of a human killer cell inhibitory receptor gene." Wilson M.J., Torkar M., Trowsdale J. Tissue Antigens 49:574-579(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298 (ISOFORM 1), VARIANT LEU-229.
[8]	"Genomic organization of novel genes encoding human killer inhibitory receptors." Chae J.H., Kim Y.M., Ahn J.M., Kim C.G., Kim J., Kim S.J., Park J.H. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-341, VARIANT GLN-34. Tissue: Placenta.
[9]	"An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells." Yu M.-C., Su L.-L., Zou L., Liu Y., Wu N., Kong L., Zhuang Z.-H., Sun L., Liu H.P., Hu J.-H., Li D., Strominger J.L., Zang J.-W., Pei G., Ge B.-X. Nat. Immunol. 9:898-907(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARRB2.
[10]	"Crystal structure of the human p58 killer cell inhibitory receptor (KIR2DL3) specific for HLA-Cw3-related MHC class I." Maenaka K., Juji T., Stuart D.I., Jones E.Y. Structure 7:391-398(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 22-245.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L41268 mRNA. Translation: AAA69869.1.
U24074 mRNA. Translation: AAC50333.1.
L76662 mRNA. Translation: AAB36590.1.
L76663 mRNA. Translation: AAB36591.1.
AF022048 mRNA. Translation: AAB95321.1.
U73395 mRNA. Translation: AAC51147.1.
BC032422 mRNA. Translation: AAH32422.1.
BC050730 mRNA. Translation: AAH50730.1.
AH006692 Genomic DNA. Translation: AAC51651.1.
AH007572 Genomic DNA. Translation: AAD24477.1.

IPI

IPI00184932.
IPI00984178.

PIR

I61725.

RefSeq

NP_056952.2. NM_015868.2.

UniGene

Hs.654608.
Hs.731378.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B6U	X-ray	3.00	A	22-245	[»]

ProteinModelPortal

P43628.

ModBase

Search...

Polymorphism databases

DMDM

1171727.

Proteomic databases

PRIDE

P43628.

Protocols and materials databases

DNASU

3804.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000342376; ENSP00000342215; ENSG00000243772.
ENST00000571420; ENSP00000461891; ENSG00000261912.
ENST00000576013; ENSP00000461171; ENSG00000263027.

GeneID

3804.

KEGG

hsa:3804.

UCSC

uc002qgx.3. human.

Organism-specific databases

CTD

3804.

GeneCards

GC19P055261.

HGNC

HGNC:6331. KIR2DL3.

MIM

604938. gene.

neXtProt

NX_P43628.

PharmGKB

PA30116.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG074353.

InParanoid

P43628.

KO

K07981.

OMA

RIFWNSL.

OrthoDB

EOG4RJG1X.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P43628.

Genevestigator

P43628.

GermOnline

ENSG00000125498. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 2 hits.

InterPro

IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view]

Pfam

PF00047. ig. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P43628.

NextBio

14943.

SOURCE

Search...

Entry information

Entry name

KI2L3_HUMAN

Accession

Primary (citable) accession number: P43628
Secondary accession number(s): O43472 , P78402, Q14944, Q14945, Q9UM51, Q9UQ70

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.