P43626 (KI2L1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Killer cell immunoglobulin-like receptor 2DL1

Alternative name(s):
CD158 antigen-like family member A
MHC class I NK cell receptor
Natural killer-associated transcript 1
Short name=NKAT-1
p58 natural killer cell receptor clones CL-42/47.11
Short name=p58 NK receptor CL-42/47.11
p58.1 MHC class-I-specific NK receptor
CD_antigen=CD158a

Gene names

Name:	KIR2DL1
Synonyms:	CD158A, NKAT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. Ref.5
Subunit structure	Interacts with ARRB2. Interacts with PTPN6; the interaction is enhanced by ARRB2. Interacts with PTPN11; the interaction is enhanced by ARRB2. Ref.5
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	immune response Traceable author statement Ref.2. Source: ProtInc natural killer cell inhibitory signaling pathway Inferred from direct assay Ref.5. Source: UniProtKB
Cellular_component	integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	receptor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.2

Chain

22 – 348

327

Killer cell immunoglobulin-like receptor 2DL1

PRO_0000015078

Regions

Topological domain

22 – 245

224

Extracellular Potential

Transmembrane

246 – 264

19

Helical; Potential

Topological domain

265 – 348

84

Cytoplasmic Potential

Domain

42 – 107

66

Ig-like C2-type 1

Domain

142 – 205

64

Ig-like C2-type 2

Amino acid modifications

Glycosylation

67

1

N-linked (GlcNAc...) Potential

Glycosylation

84

1

N-linked (GlcNAc...) Potential

Glycosylation

144

1

N-linked (GlcNAc...) Potential

Glycosylation

178

1

N-linked (GlcNAc...) Potential

Disulfide bond

49 ↔ 100

Disulfide bond

149 ↔ 198

Natural variations

Natural variant

5

1

V → F. Ref.4
Corresponds to variant rs2304224 [ dbSNP | Ensembl ].

VAR_003949

Natural variant

9

1

A → V.
Corresponds to variant rs3810343 [ dbSNP | Ensembl ].

VAR_061332

Natural variant

37

1

P → R.
Corresponds to variant rs35509911 [ dbSNP | Ensembl ].

VAR_003950

Natural variant

66

1

F → Y.
Corresponds to variant rs673568 [ dbSNP | Ensembl ].

VAR_056091

Natural variant

111

1

V → L.
Corresponds to variant rs687885 [ dbSNP | Ensembl ].

VAR_061333

Natural variant

135

1

P → L.

VAR_003951

Natural variant

175

1

P → T.

VAR_010331

Natural variant

184

1

D → N.

VAR_010332

Natural variant

203

1

H → R.

VAR_010333

Natural variant

237

1

K → E.

VAR_010334

Natural variant

266

1

R → C.

VAR_010335

Experimental info

Mutagenesis

302

1

Y → A: Abolishes interaction with ARRB2; when associated with A-332. Diminishes interaction with ARRB2. Ref.5

Mutagenesis

332

1

Y → A: Abolishes interaction with ARRB2; when associated with A-302. Ref.5

Secondary structure

1

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348

Helix Strand Turn

Details...

Beta strand

30 – 35

6

Beta strand

37 – 40

4

Beta strand

45 – 53

9

Beta strand

56 – 66

11

Beta strand

68 – 73

6

Beta strand

75 – 77

3

Beta strand

80 – 89

10

Helix

92 – 94

3

Beta strand

96 – 104

9

Beta strand

118 – 123

6

Beta strand

130 – 135

6

Beta strand

137 – 140

4

Beta strand

144 – 153

10

Beta strand

156 – 162

7

Beta strand

169 – 172

4

Beta strand

174 – 177

4

Beta strand

180 – 189

10

Beta strand

194 – 202

9

Beta strand

205 – 209

5

Beta strand

216 – 221

6

Sequences

	Sequence		Length	Mass (Da)
	P43626 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 3DFBF2D6FDCE1D1E	FASTA	348	38,505
	10 20 30 40 50 60 MSLLVVSMAC VGFFLLQGAW PHEGVHRKPS LLAHPGPLVK SEETVILQCW SDVMFEHFLL 70 80 90 100 110 120 HREGMFNDTL RLIGEHHDGV SKANFSISRM TQDLAGTYRC YGSVTHSPYQ VSAPSDPLDI 130 140 150 160 170 180 VIIGLYEKPS LSAQPGPTVL AGENVTLSCS SRSSYDMYHL SREGEAHERR LPAGPKVNGT 190 200 210 220 230 240 FQADFPLGPA THGGTYRCFG SFHDSPYEWS KSSDPLLVSV TGNPSNSWPS PTEPSSKTGN 250 260 270 280 290 300 PRHLHILIGT SVVIILFILL FFLLHRWCSN KKNAAVMDQE SAGNRTANSE DSDEQDPQEV 310 320 330 340 TYTQLNHCVF TQRKITRPSQ RPKTPPTDII VYTELPNAES RSKVVSCP

References

[1]	"Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells." Colonna M., Samaridis J. Science 268:405-408(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Natural killer cell.
[2]	"Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains." Wagtmann N., Biassoni R., Cantoni C., Verdiani S., Malnati M.S., Vitale M., Bottino C., Moretta L., Moretta A., Long E.O. Immunity 2:439-449(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-45. Tissue: Natural killer cell.
[3]	"Human diversity in killer cell inhibitory receptor genes." Uhrberg M., Valiante N.M., Shum B.P., Shilling H.G., Lienert-Weidenbach K., Corliss B., Tyan D., Lanier L.L., Parham P. Immunity 7:753-763(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
[4]	"Variants of KIR identified in Japanese donors." Yawata N., Yawata M., Parham P. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-5.
[5]	"An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells." Yu M.-C., Su L.-L., Zou L., Liu Y., Wu N., Kong L., Zhuang Z.-H., Sun L., Liu H.P., Hu J.-H., Li D., Strominger J.L., Zang J.-W., Pei G., Ge B.-X. Nat. Immunol. 9:898-907(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARRB2; PTPN6 AND PTPN11, MUTAGENESIS OF TYR-302 AND TYR-332.
[6]	"Structure of the inhibitory receptor for human natural killer cells resembles haematopoietic receptors." Fan Q.R., Mosyak L., Winter C.C., Wagtmann N., Long E.O., Wiley D.C. Nature 389:96-100(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-221.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L41267 mRNA. Translation: AAA69868.1.
U24076 mRNA. Translation: AAC50335.1.
U24078 mRNA. Translation: AAC50337.1.
AF022045 mRNA. Translation: AAB95318.1.
AY789055 mRNA. Translation: AAX23100.1.

IPI

IPI00302335.

PIR

A56247.

RefSeq

NP_055033.2. NM_014218.2.

UniGene

Hs.512572.
Hs.654605.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IM9	X-ray	2.80	D	22-245	[»]
1NKR	X-ray	1.70	A	22-221	[»]

ProteinModelPortal

P43626.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000336769.

PTM databases

PhosphoSite

P43626.

Polymorphism databases

DMDM

1171726.

Proteomic databases

PaxDb

P43626.

PRIDE

P43626.

Protocols and materials databases

DNASU

3802.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000336077; ENSP00000336769; ENSG00000125498.
ENST00000576294; ENSP00000458867; ENSG00000263208.

GeneID

3802.

KEGG

hsa:3802.

Organism-specific databases

CTD

3802.

GeneCards

GC19P055283.

HGNC

HGNC:6329. KIR2DL1.

HPA

HPA049255.

MIM

604936. gene.

neXtProt

NX_P43626.

PharmGKB

PA30114.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG82473.

HOGENOM

HOG000234396.

HOVERGEN

HBG074353.

KO

K07981.

OrthoDB

EOG4Z8XX8.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P43626.

CleanEx

HS_KIR2DL1.

Genevestigator

P43626.

GermOnline

ENSG00000125498. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 2 hits.

InterPro

IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view]

Pfam

PF00047. ig. 2 hits.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P43626.

GenomeRNAi

3802.

NextBio

14935.

SOURCE

Search...

Entry information

Entry name

KI2L1_HUMAN

Accession

Primary (citable) accession number: P43626
Secondary accession number(s): O43470, Q32WE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.