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Protein

Putative cystathionine beta-lyase

Gene

IRC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from L-cystathionine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative cystathionine beta-lyase (IRC7), Cystathionine beta-lyase (STR3)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from L-cystathionine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YFR055W-MONOMER.
BRENDAi4.4.1.8. 984.
UniPathwayiUPA00051; UER00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cystathionine beta-lyase (EC:4.4.1.8)
Short name:
CBL
Alternative name(s):
Beta-cystathionase
Cysteine lyase
Increased recombination centers protein 7
Gene namesi
Name:IRC7
Ordered Locus Names:YFR055W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR055W.
SGDiS000001952. IRC7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147741 – 340Putative cystathionine beta-lyaseAdd BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei208N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiP43623.

Interactioni

Protein-protein interaction databases

BioGridi31212. 25 interactors.
DIPiDIP-5187N.
MINTiMINT-488578.

Structurei

3D structure databases

ProteinModelPortaliP43623.
SMRiP43623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075611.
HOGENOMiHOG000246416.
InParanoidiP43623.
KOiK01760.
OMAiPTHFAFQ.
OrthoDBiEOG092C2LGC.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006233. Cys_b_lyase_bac.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01324. cysta_beta_ly_B. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDRTELSKF GITTQLSVIG RNPDEQSGFV NPPLYKGSTI ILKKLSDLEQ
60 70 80 90 100
RKGRFYGTAG SPTIDNLENA WTHLTGGAGT VLSASGLGSI SLALLALSKA
110 120 130 140 150
GDHILMTDSV YVPTRMLCDG LLAKFGVETD YYDPSIGKDI EKLVKPNTTV
160 170 180 190 200
IFLESPGSGT MEVQDIPALV SVAKKHGIKT ILDNTWATPL FFDAHAHGID
210 220 230 240 250
ISVEAGTKYL GGHSDLLIGL ASANEECWPL LRSTYDAMAM LPGAEDCQLA
260 270 280 290 300
LRGMRTLHLR LKEVERKALD LAAWLGNRDE VEKVLHPAFE DCPGHEYWVR
310 320 330 340
DYKGSSGLFS IVLKNGFTRA GLEKMVEGMK VLQLGFSWGG
Length:340
Mass (Da):36,971
Last modified:November 1, 1995 - v1
Checksum:i75FF4C7BDA038B8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09295.1.
BK006940 Genomic DNA. Translation: DAA12497.1.
PIRiS56311.
RefSeqiNP_116714.1. NM_001180021.1.

Genome annotation databases

EnsemblFungiiBAA09295; BAA09295; BAA09295.
YFR055W; YFR055W; YFR055W.
GeneIDi850616.
KEGGisce:YFR055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09295.1.
BK006940 Genomic DNA. Translation: DAA12497.1.
PIRiS56311.
RefSeqiNP_116714.1. NM_001180021.1.

3D structure databases

ProteinModelPortaliP43623.
SMRiP43623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31212. 25 interactors.
DIPiDIP-5187N.
MINTiMINT-488578.

Proteomic databases

PRIDEiP43623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09295; BAA09295; BAA09295.
YFR055W; YFR055W; YFR055W.
GeneIDi850616.
KEGGisce:YFR055W.

Organism-specific databases

EuPathDBiFungiDB:YFR055W.
SGDiS000001952. IRC7.

Phylogenomic databases

GeneTreeiENSGT00550000075611.
HOGENOMiHOG000246416.
InParanoidiP43623.
KOiK01760.
OMAiPTHFAFQ.
OrthoDBiEOG092C2LGC.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00078.
BioCyciYEAST:YFR055W-MONOMER.
BRENDAi4.4.1.8. 984.

Miscellaneous databases

PROiP43623.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006233. Cys_b_lyase_bac.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01324. cysta_beta_ly_B. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETC_YEAST
AccessioniPrimary (citable) accession number: P43623
Secondary accession number(s): D6VTT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.