Reviewed, UniProtKB/Swiss-Prot P43623 (METC_YEAST)

Names and origin

Protein names

Recommended name:
    Putative cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase
    Increased recombination centers protein 7

Gene names

Name:	IRC7
Ordered Locus Names:	YFR055W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Miscellaneous	Present with 2610 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular copper ion homeostasis Inferred from expression pattern. Source: SGD methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mitotic recombination Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Putative cystathionine beta-lyase		PRO_0000114774
Amino acid modifications
	Modified residue	208	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43623-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 75FF4C7BDA038B8F	FASTA	340	36,971
	10 20 30 40 50 60 MIDRTELSKF GITTQLSVIG RNPDEQSGFV NPPLYKGSTI ILKKLSDLEQ RKGRFYGTAG 70 80 90 100 110 120 SPTIDNLENA WTHLTGGAGT VLSASGLGSI SLALLALSKA GDHILMTDSV YVPTRMLCDG 130 140 150 160 170 180 LLAKFGVETD YYDPSIGKDI EKLVKPNTTV IFLESPGSGT MEVQDIPALV SVAKKHGIKT 190 200 210 220 230 240 ILDNTWATPL FFDAHAHGID ISVEAGTKYL GGHSDLLIGL ASANEECWPL LRSTYDAMAM 250 260 270 280 290 300 LPGAEDCQLA LRGMRTLHLR LKEVERKALD LAAWLGNRDE VEKVLHPAFE DCPGHEYWVR 310 320 330 340 DYKGSSGLFS IVLKNGFTRA GLEKMVEGMK VLQLGFSWGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed: 8686379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09295.1.
PIR	S56311.
RefSeq	NP_116714.1.
3D structure databases
HSSP	HSSP built from PDB template 1CL2 based on UniProtKB P06721.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5187N.
IntAct	P43623. 2 interactions.
STRING	P43623.
Proteomic databases
PRIDE	P43623.
Genome annotation databases
Ensembl	YFR055W; YFR055W; YFR055W; Saccharomyces cerevisiae. [Genome view]
GeneID	850616.
GenomeReviews	Gene locus YFR055W in contig D50617_GR.
KEGG	sce:YFR055W.
NMPDR	fig|4932.3.peg.2351.
Organism-specific databases
CYGD	YFR055w.
SGD	S000001952. YFR055W.
Phylogenomic databases
HOGENOM	P43623.
OMA	VIDDNTW.
Enzyme and pathway databases
BRENDA	4.4.1.8. 250.
Gene expression databases
ArrayExpress	P43623.
Genevestigator	P43623.
GermOnline	YFR055W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006233. Cys_b_lyase_bac. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808:SF8. Cys_b_lyase_bac. 1 hit. PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966511.

Entry information

Entry name

METC_YEAST

Accession

Primary (citable) accession number: P43623

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)