P43620 (RMD8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sporulation protein RMD8

Alternative name(s):
Required for meiotic nuclear division protein 8

Gene names

Name:	RMD8
Ordered Locus Names:	YFR048W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for sporulation. Ref.4
Subcellular location	Membrane; Single-pass membrane protein Potential.
Miscellaneous	Present with 656 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RMD1/sif2 family.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 662	662	Sporulation protein RMD8		PRO_0000202698
Regions
	Transmembrane	630 – 647	18	Helical; Potential
Amino acid modifications
	Modified residue	175	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P43620 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1B5B7964A6520FF1	FASTA	662	75,914
	10 20 30 40 50 60 MSYKANQPSP GEMPKRSPSI LVTDARTSKN RMSAPFAGHA AGSRKNMENA GVTKSQGVRS 70 80 90 100 110 120 SAIGPSPLQS FTHPRRRSSG RFSDISIDNI LSDNSDIPSA RREERLSSSS SDRPRQYERL 130 140 150 160 170 180 SSRRKMINPL PPRTSKTSQK LVLIPEDDNL NHFQTLPTNA LDRQRPKVGS MKSNSFDRLP 190 200 210 220 230 240 RYSKEKSMAR ITAYNVADGF NLNQLYKFLQ ETHEVSPRLY DECLYVAYTL PLLPGKGGFR 250 260 270 280 290 300 IKSNLSKKTM GGKTLIDNLI DTSEQRDHHY EYYSGVETVE DANNNYELET SGNNNNANQD 310 320 330 340 350 360 TTTVPDHLPN PVGQQDSFNP MEPQFFAEET PLEIEKRERT ERINMLKKEE NDSDASCGND 370 380 390 400 410 420 NNNKNNDKSK LYAVEGNDQY VQSSRSPASP SSISTPSPPS SSQNDFDRVY KMHRDNDHEG 430 440 450 460 470 480 NDRHAEIFIF HYGVIVFWNF TEIQEKNILG DITFADYKNL MIRPLDEQDI ETEQFHFEYD 490 500 510 520 530 540 RDTERPRIFN DIVTLRSGDH IIELTLSHAI AQSSKLSRFE SRISPILISV TKLPKRLALY 550 560 570 580 590 600 GTLGLKREQL LKKSGKLFKL RVDVNLSSTI LDTPEFFWSF EPSLHPLYVA MREYLEIDQR 610 620 630 640 650 660 VQVLNDRCKV FLEFFDICVD SVAERNMARV TWWFILVILF GVIFSLTEIF VRYVIIHRHT ST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae." Enyenihi A.H., Saunders W.S. Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09287.1. BK006940 Genomic DNA. Translation: DAA12491.1.
PIR	S56303.
RefSeq	NP_683716.1. NM_001180013.1.
3D structure databases
ProteinModelPortal	P43620.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43620. 2 interactions.
MINT	MINT-2788460.
STRING	4932.YFR048W.
Proteomic databases
PaxDb	P43620.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR048W; YFR048W; YFR048W.
GeneID	850609.
KEGG	sce:YFR048W.
Organism-specific databases
CYGD	YFR048w.
SGD	S000001944. RMD8.
Phylogenomic databases
eggNOG	COG1723.
GeneTree	ENSGT00550000075849.
HOGENOM	HOG000190628.
OMA	RVQVLND.
OrthoDB	EOG42Z7ZH.
Gene expression databases
Genevestigator	P43620.
GermOnline	YFR048W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003734. DUF155. [Graphical view]
PANTHER	PTHR16255. PTHR16255. 1 hit.
Pfam	PF02582. DUF155. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966493.

Entry information

Entry name

RMD8_YEAST

Accession

Primary (citable) accession number: P43620
Secondary accession number(s): D6VTT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program