Nicotinate-nucleotide pyrophosphorylase [carboxylating] - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P43619 (NADC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
EC=2.4.2.19

Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name=QAPRTase

Gene names

Name:	BNA6
Synonyms:	QPT1
Ordered Locus Names:	YFR047C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the catabolism of quinolinic acid (QA) By similarity.
Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate + CO₂ = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate. Ref.4
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1. Ref.4
Subunit structure	Hexamer formed by 3 homodimers. Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 2660 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NadC/ModD family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm Nucleus
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	de novo NAD biosynthetic process from tryptophan Inferred from genetic interaction Ref.4. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular_function	nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-11793,EBI-11793

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

PRO_0000155956

Regions

Region

142 – 144

Substrate binding

Region

256 – 258

Substrate binding

Sites

Binding site

107

Substrate

Binding site

166

Substrate

Binding site

176

Substrate

Binding site

206

Substrate By similarity

Binding site

227

Substrate

Secondary structure

295

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P43619 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0680BB5DDC095050
Show »

FASTA

295

32,365

        10         20         30         40         50         60 
MPVYEHLLPV NGAWRQDVTN WLSEDVPSFD FGGYVVGSDL KEANLYCKQD GMLCGVPFAQ 

        70         80         90        100        110        120 
EVFNQCELQV EWLFKEGSFL EPSKNDSGKI VVAKITGPAK NILLAERTAL NILSRSSGIA 

       130        140        150        160        170        180 
TASHKIISLA RSTGYKGTIA GTRKTTPGLR RLEKYSMLVG GCDTHRYDLS SMVMLKDNHI 

       190        200        210        220        230        240 
WATGSITNAV KNARAVCGFA VKIEVECLSE DEATEAIEAG ADVIMLDNFK GDGLKMCAQS 

       250        260        270        280        290 
LKNKWNGKKH FLLECSGGLN LDNLEEYLCD DIDIYSTSSI HQGTPVIDFS LKLAH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae." Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J. FEBS Lett. 517:97-102(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, PATHWAY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Comprehensive X-ray structural studies of the quinolinate phosphoribosyl transferase (BNA6) from Saccharomyces cerevisiae." di Luccio E., Wilson D.K. Biochemistry 47:4039-4050(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-295 IN COMPLEX WITH SUBSTRATE, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50617 Genomic DNA. Translation: BAA09286.1.
BK006940 Genomic DNA. Translation: DAA12490.1.

PIR

S56302.

RefSeq

NP_602317.3. NM_001180012.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C2E	X-ray	1.90	A	2-295	[»]
3C2F	X-ray	2.35	A	2-295	[»]
3C2O	X-ray	2.30	A	2-295	[»]
3C2R	X-ray	2.40	A/B	1-295	[»]
3C2V	X-ray	2.29	A	2-295	[»]

ProteinModelPortal

P43619.

SMR

P43619. Positions 2-294.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1569N.

IntAct

P43619. 3 interactions.

MINT

MINT-394142.

STRING

4932.YFR047C.

Proteomic databases

PaxDb

P43619.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFR047C; YFR047C; YFR047C.

GeneID

850608.

KEGG

sce:YFR047C.
sce:YFR049W.

Organism-specific databases

CYGD

YFR047c.

SGD

S000001943. BNA6.

Phylogenomic databases

eggNOG

COG0157.

GeneTree

ENSGT00390000002761.

HOGENOM

HOG000224023.

K00767.

OMA

HIWATGS.

OrthoDB

EOG4FN7SR.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-8162.

UniPathway

UPA00253; UER00331.

Gene expression databases

Genevestigator

P43619.

GermOnline

YFR047C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.

InterPro

IPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]

Pfam

PF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]

PIRSF

PIRSF006250. NadC_ModD. 1 hit.

SUPFAM

SSF51690. Q_phspho_trans. 1 hit.

TIGRFAMs

TIGR00078. nadC. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P43619.

NextBio

966490.

Entry information

Entry name

NADC_YEAST

Accession

Primary (citable) accession number: P43619
Secondary accession number(s): D6VTT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents