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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

BNA6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Miscellaneous

Present with 2660 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (BNA6)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107Substrate1 Publication1
Binding sitei166Substrate1 Publication1
Binding sitei176Substrate1 Publication1
Binding sitei206SubstrateBy similarity1
Binding sitei227Substrate1 Publication1

GO - Molecular functioni

  • nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: SGD

GO - Biological processi

  • 'de novo' NAD biosynthetic process from tryptophan Source: SGD
  • quinolinate catabolic process Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YFR047C-MONOMER
YEAST:YFR047C-MONOMER
BRENDAi2.4.2.19 984
ReactomeiR-SCE-196807 Nicotinate metabolism
UniPathwayiUPA00253; UER00331

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:BNA6
Synonyms:QPT1
Ordered Locus Names:YFR047C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR047C
SGDiS000001943 BNA6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001559561 – 295Nicotinate-nucleotide pyrophosphorylase [carboxylating]Add BLAST295

Proteomic databases

MaxQBiP43619
PaxDbiP43619
PRIDEiP43619
TopDownProteomicsiP43619

PTM databases

iPTMnetiP43619

Interactioni

Subunit structurei

Hexamer formed by 3 homodimers.1 Publication

Protein-protein interaction databases

BioGridi31205, 42 interactors
DIPiDIP-1569N
IntActiP43619, 3 interactors
MINTiP43619
STRINGi4932.YFR047C

Structurei

Secondary structure

1295
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Beta strandi10 – 12Combined sources3
Helixi13 – 25Combined sources13
Helixi31 – 36Combined sources6
Beta strandi40 – 47Combined sources8
Beta strandi49 – 52Combined sources4
Helixi56 – 65Combined sources10
Beta strandi69 – 74Combined sources6
Helixi82 – 84Combined sources3
Beta strandi85 – 88Combined sources4
Beta strandi90 – 98Combined sources9
Helixi99 – 132Combined sources34
Beta strandi137 – 140Combined sources4
Turni147 – 149Combined sources3
Helixi150 – 159Combined sources10
Turni169 – 171Combined sources3
Beta strandi172 – 175Combined sources4
Helixi177 – 183Combined sources7
Helixi186 – 197Combined sources12
Beta strandi199 – 201Combined sources3
Beta strandi203 – 206Combined sources4
Beta strandi208 – 210Combined sources3
Helixi211 – 219Combined sources9
Beta strandi222 – 225Combined sources4
Beta strandi252 – 256Combined sources5
Beta strandi273 – 276Combined sources4
Helixi278 – 280Combined sources3
Turni281 – 283Combined sources3
Beta strandi289 – 293Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C2EX-ray1.90A2-295[»]
3C2FX-ray2.35A2-295[»]
3C2OX-ray2.30A2-295[»]
3C2RX-ray2.40A/B1-295[»]
3C2VX-ray2.29A2-295[»]
ProteinModelPortaliP43619
SMRiP43619
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43619

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni142 – 144Substrate binding3
Regioni256 – 258Substrate binding3

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002761
HOGENOMiHOG000224023
InParanoidiP43619
KOiK00767
OMAiVIMLDNM
OrthoDBiEOG092C4NJQ

Family and domain databases

CDDicd01572 QPRTase, 1 hit
Gene3Di3.20.20.70, 1 hit
3.90.1170.20, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR004393 NadC
IPR027277 NadC/ModD
IPR036068 Nicotinate_pribotase_like_C
IPR037128 Quinolinate_PRibosylTase_N_sf
IPR002638 Quinolinate_PRibosylTrfase_C
IPR022412 Quinolinate_PRibosylTrfase_N
PANTHERiPTHR32179 PTHR32179, 1 hit
PfamiView protein in Pfam
PF01729 QRPTase_C, 1 hit
PF02749 QRPTase_N, 1 hit
PIRSFiPIRSF006250 NadC_ModD, 1 hit
SUPFAMiSSF51690 SSF51690, 1 hit
TIGRFAMsiTIGR00078 nadC, 1 hit

Sequencei

Sequence statusi: Complete.

P43619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVYEHLLPV NGAWRQDVTN WLSEDVPSFD FGGYVVGSDL KEANLYCKQD
60 70 80 90 100
GMLCGVPFAQ EVFNQCELQV EWLFKEGSFL EPSKNDSGKI VVAKITGPAK
110 120 130 140 150
NILLAERTAL NILSRSSGIA TASHKIISLA RSTGYKGTIA GTRKTTPGLR
160 170 180 190 200
RLEKYSMLVG GCDTHRYDLS SMVMLKDNHI WATGSITNAV KNARAVCGFA
210 220 230 240 250
VKIEVECLSE DEATEAIEAG ADVIMLDNFK GDGLKMCAQS LKNKWNGKKH
260 270 280 290
FLLECSGGLN LDNLEEYLCD DIDIYSTSSI HQGTPVIDFS LKLAH
Length:295
Mass (Da):32,365
Last modified:November 1, 1995 - v1
Checksum:i0680BB5DDC095050
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09286.1
BK006940 Genomic DNA Translation: DAA12490.1
PIRiS56302
RefSeqiNP_602317.3, NM_001180012.3

Genome annotation databases

EnsemblFungiiBAA09286; BAA09286; BAA09286
YFR047C; YFR047C; YFR047C
GeneIDi850608
KEGGisce:YFR047C

Similar proteinsi

Entry informationi

Entry nameiNADC_YEAST
AccessioniPrimary (citable) accession number: P43619
Secondary accession number(s): D6VTT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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