Nicotinate-nucleotide pyrophosphorylase [carboxylating] - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P43619 (NADC_YEAST)

Last modified November 3, 2009. Version 89. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Nicotinate-nucleotide pyrophosphorylase [carboxylating]
    EC=2.4.2.19
Alternative name(s):
    Quinolinate phosphoribosyltransferase [decarboxylating]
      Short name=QAPRTase

Gene names

Name:	BNA6
Synonyms:	QPT1
Ordered Locus Names:	YFR047C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Nicotinate D-ribonucleotide + diphosphate + CO₂ = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate. Ref.3
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1. Ref.3
Subcellular location	Cytoplasm. Nucleus. Ref.4
Miscellaneous	Present with 2660 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the nadC/modD family.

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Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm Nucleus
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	de novo NAD biosynthetic process from tryptophan Ref.3 Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct nicotinate-nucleotide diphosphorylase (carboxylating) activity Ref.3 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-11793,EBI-11793
SRP1	Q02821	1	EBI-11793,EBI-1797

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

PRO_0000155956

Secondary structure

295

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P43619-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0680BB5DDC095050
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FASTA

295

32,365

        10         20         30         40         50         60 
MPVYEHLLPV NGAWRQDVTN WLSEDVPSFD FGGYVVGSDL KEANLYCKQD GMLCGVPFAQ 

        70         80         90        100        110        120 
EVFNQCELQV EWLFKEGSFL EPSKNDSGKI VVAKITGPAK NILLAERTAL NILSRSSGIA 

       130        140        150        160        170        180 
TASHKIISLA RSTGYKGTIA GTRKTTPGLR RLEKYSMLVG GCDTHRYDLS SMVMLKDNHI 

       190        200        210        220        230        240 
WATGSITNAV KNARAVCGFA VKIEVECLSE DEATEAIEAG ADVIMLDNFK GDGLKMCAQS 

       250        260        270        280        290 
LKNKWNGKKH FLLECSGGLN LDNLEEYLCD DIDIYSTSSI HQGTPVIDFS LKLAH

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed: 8686379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae." Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J. FEBS Lett. 517:97-102(2002) [PubMed: 12062417] [Abstract] Cited for: CATALYTIC ACTIVITY, PATHWAY.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases

D50617 Genomic DNA. Translation: BAA09286.1.

PIR

S56302.

RefSeq

NP_602317.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C2E	X-ray	1.90	A	2-295	[»]
3C2F	X-ray	2.35	A	2-295	[»]
3C2O	X-ray	2.30	A	2-295	[»]
3C2R	X-ray	2.40	A/B	1-295	[»]
3C2V	X-ray	2.29	A	2-295	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1569N.

IntAct

P43619. 5 interactions.

STRING

P43619.

Proteomic databases

PRIDE

P43619.

Genome annotation databases

Ensembl

YFR047C; YFR047C; YFR047C; Saccharomyces cerevisiae. [Genome view]

GeneID

850608.

GenomeReviews

Gene locus YFR047C in contig D50617_GR.

KEGG

sce:YFR047C.

NMPDR

fig|4932.3.peg.2341.

Organism-specific databases

CYGD

YFR047c.

SGD

S000001943. BNA6.

Phylogenomic databases

HOGENOM

P43619.

OMA

PEELHPT.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-8162.

BRENDA

2.4.2.19. 250.

Gene expression databases

ArrayExpress

P43619.

Genevestigator

P43619.

GermOnline

YFR047C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR002638. Q_phspho_trans.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

Pfam

PF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]

ProDom

PD003988. Q_phspho_trans. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00078. nadC. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

966490.

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Entry information

Entry name

NADC_YEAST

Accession

Primary (citable) accession number: P43619

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents