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UniProtKB - P43619 (NADC_YEAST)

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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

BNA6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Miscellaneous

Present with 2660 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (BNA6)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107Substrate1 Publication1
Binding sitei166Substrate1 Publication1
Binding sitei176Substrate1 Publication1
Binding sitei206SubstrateBy similarity1
Binding sitei227Substrate1 Publication1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: SGD
Complete GO annotation...

GO - Biological processi

  • 'de novo' NAD biosynthetic process from tryptophan Source: SGD
  • quinolinate catabolic process Source: GO_Central
Complete GO annotation...

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YFR047C-MONOMER.
YEAST:YFR047C-MONOMER.
BRENDAi2.4.2.19. 984.
ReactomeiR-SCE-196807. Nicotinate metabolism.
UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:BNA6
Synonyms:QPT1
Ordered Locus Names:YFR047C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR047C.
SGDiS000001943. BNA6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001559561 – 295Nicotinate-nucleotide pyrophosphorylase [carboxylating]Add BLAST295

Proteomic databases

MaxQBiP43619.
PRIDEiP43619.
TopDownProteomicsiP43619.

PTM databases

iPTMnetiP43619.

Interactioni

Subunit structurei

Hexamer formed by 3 homodimers.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-11793,EBI-11793

GO - Molecular functioni

  • identical protein binding Source: IntAct
Complete GO annotation...

Protein-protein interaction databases

BioGridi31205. 41 interactors.
DIPiDIP-1569N.
IntActiP43619. 3 interactors.
MINTiMINT-394142.
STRINGi4932.YFR047C.

Structurei

Secondary structure

1295
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Beta strandi10 – 12Combined sources3
Helixi13 – 25Combined sources13
Helixi31 – 36Combined sources6
Beta strandi40 – 47Combined sources8
Beta strandi49 – 52Combined sources4
Helixi56 – 65Combined sources10
Beta strandi69 – 74Combined sources6
Helixi82 – 84Combined sources3
Beta strandi85 – 88Combined sources4
Beta strandi90 – 98Combined sources9
Helixi99 – 132Combined sources34
Beta strandi137 – 140Combined sources4
Turni147 – 149Combined sources3
Helixi150 – 159Combined sources10
Turni169 – 171Combined sources3
Beta strandi172 – 175Combined sources4
Helixi177 – 183Combined sources7
Helixi186 – 197Combined sources12
Beta strandi199 – 201Combined sources3
Beta strandi203 – 206Combined sources4
Beta strandi208 – 210Combined sources3
Helixi211 – 219Combined sources9
Beta strandi222 – 225Combined sources4
Beta strandi252 – 256Combined sources5
Beta strandi273 – 276Combined sources4
Helixi278 – 280Combined sources3
Turni281 – 283Combined sources3
Beta strandi289 – 293Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C2EX-ray1.90A2-295[»]
3C2FX-ray2.35A2-295[»]
3C2OX-ray2.30A2-295[»]
3C2RX-ray2.40A/B1-295[»]
3C2VX-ray2.29A2-295[»]
ProteinModelPortaliP43619.
SMRiP43619.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43619.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni142 – 144Substrate binding3
Regioni256 – 258Substrate binding3

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002761.
HOGENOMiHOG000224023.
InParanoidiP43619.
KOiK00767.
OMAiVIMLDNM.
OrthoDBiEOG092C4NJQ.

Family and domain databases

CDDicd01572. QPRTase. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
PANTHERiPTHR32179. PTHR32179. 1 hit.
PfamiView protein in Pfam
PF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

P43619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVYEHLLPV NGAWRQDVTN WLSEDVPSFD FGGYVVGSDL KEANLYCKQD 
        60         70         80         90        100
GMLCGVPFAQ EVFNQCELQV EWLFKEGSFL EPSKNDSGKI VVAKITGPAK 
       110        120        130        140        150
NILLAERTAL NILSRSSGIA TASHKIISLA RSTGYKGTIA GTRKTTPGLR 
       160        170        180        190        200
RLEKYSMLVG GCDTHRYDLS SMVMLKDNHI WATGSITNAV KNARAVCGFA 
       210        220        230        240        250
VKIEVECLSE DEATEAIEAG ADVIMLDNFK GDGLKMCAQS LKNKWNGKKH 
       260        270        280        290 
FLLECSGGLN LDNLEEYLCD DIDIYSTSSI HQGTPVIDFS LKLAH
Length:295
Mass (Da):32,365
Last modified:November 1, 1995 - v1
Checksum:i0680BB5DDC095050
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09286.1.
BK006940 Genomic DNA. Translation: DAA12490.1.
PIRiS56302.
RefSeqiNP_602317.3. NM_001180012.3.

Genome annotation databases

EnsemblFungiiBAA09286; BAA09286; BAA09286.
YFR047C; YFR047C; YFR047C.
GeneIDi850608.
KEGGisce:YFR047C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNADC_YEAST
AccessioniPrimary (citable) accession number: P43619
Secondary accession number(s): D6VTT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names