P43618 (CNN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinetochore-associated protein CNN1

Alternative name(s):
Co-purified with NNF1 protein 1

Gene names

Name:	CNN1
Ordered Locus Names:	YFR046C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the kinetochore. Interacts with NNF1. Ref.5
Subcellular location	Nucleus. Chromosome › centromere › kinetochore Ref.6.
Miscellaneous	Present with 1300 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from physical interaction Ref.5PubMed 9520439. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell kinetochore Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.6. Source: SGD
Molecular_function	centromeric DNA binding Inferred from direct assay PubMed 22561345PubMed 22561346. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 361

361

Kinetochore-associated protein CNN1

PRO_0000202697

Experimental info

Sequence conflict

8

1

A → T in AAT92741. Ref.2

Secondary structure

1

.

.

.

361

Helix Strand Turn

Details...

Helix

63 – 78

16

Sequences

	Sequence		Length	Mass (Da)
	P43618 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 48CEEC087A056D42	FASTA	361	41,313
	10 20 30 40 50 60 MSTPRKAAGN NENTEVSEIR TPFRERALEE QRLKDEVLIR NTPGYRKLLS ASTKSHDILN 70 80 90 100 110 120 KDPNEVRSFL QDLSQVLARK SQGNDTTTNK TQARNLIDEL AYEESQPEEN ELLRSRSEKL 130 140 150 160 170 180 TDNNIGNETQ PDYTSLSQTV FAKLQERDKG LKSRKIDPII IQDVPTTGHE DELTVHSPDK 190 200 210 220 230 240 ANSISMEVLR TSPSIGMDQV DEPPVRDPVP ISITQQEEPL SEDLPSDDKE ETEEAENEDY 250 260 270 280 290 300 SFENTSDENL DDIGNDPIRL NVPAVRRSSI KPLQIMDLKH LTRQFLNENR IILPKQTWST 310 320 330 340 350 360 IQEESLNIMD FLKQKIGTLQ KQELVDSFID MGIINNVDDM FELAHELLPL ELQSRIESYL F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes." De Wulf P., McAinsh A.D., Sorger P.K. Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH NNF1.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50617 Genomic DNA. Translation: BAA09285.1.
AY692722 Genomic DNA. Translation: AAT92741.1.
BK006940 Genomic DNA. Translation: DAA12489.1.

PIR

S56301.

RefSeq

NP_116704.1. NM_001180011.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GEQ	X-ray	2.01	E/F	60-84	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5186N.

IntAct

P43618. 1 interaction.

MINT

MINT-557134.

STRING

4932.YFR046C.

Proteomic databases

PaxDb

P43618.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFR046C; YFR046C; YFR046C.

GeneID

850607.

KEGG

sce:YFR046C.

Organism-specific databases

CYGD

YFR046c.

SGD

S000001942. CNN1.

Phylogenomic databases

eggNOG

NOG132497.

OrthoDB

EOG4TJ03X.

Gene expression databases

Genevestigator

P43618.

GermOnline

YFR046C. Saccharomyces cerevisiae.

Family and domain databases

ProtoNet

Search...

Other

NextBio

966487.

Entry information

Entry name

CNN1_YEAST

Accession

Primary (citable) accession number: P43618
Secondary accession number(s): D6VTS9, Q6B2K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program