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Protein

Inner kinetochore subunit CNN1

Gene

CNN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly (PubMed:22561346). CNN1 is important for the recruitment of the outer kinetochore Ndc80 complex (PubMed:23334295).2 Publications

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • centromeric DNA binding Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • negative regulation of NMS complex assembly Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30493-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Inner kinetochore subunit CNN1Curated
Alternative name(s):
CENP-T homolog1 Publication
Co-purified with NNF1 protein 1
Constitutive centromere-associated network protein CNN1Curated
Gene namesi
Name:CNN1
Ordered Locus Names:YFR046C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR046C
SGDiS000001942 CNN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi74S → A: Increases interaction with SPC24-SPC25. 1 Publication1
Mutagenesisi74S → D: Abolishes interaction with SPC24-SPC25. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026971 – 361Inner kinetochore subunit CNN1Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Phosphoserine; by CDK11 Publication1
Modified residuei14Phosphothreonine; by CDK1 and MPS11 Publication1
Modified residuei17Phosphoserine; by CDK1 and MPS11 Publication1
Modified residuei21Phosphothreonine; by CDK11 Publication1
Modified residuei42Phosphothreonine; by CDK11 Publication1
Modified residuei50Phosphoserine; by CDK1 and MPS11 Publication1
Modified residuei52Phosphoserine; by CDK11 Publication1
Modified residuei53Phosphothreonine; by MPS11 Publication1
Modified residuei55Phosphoserine; by CDK11 Publication1
Modified residuei74Phosphoserine; by CDK1 and MPS11 Publication1
Modified residuei86Phosphothreonine; by MPS11 Publication1
Modified residuei88Phosphothreonine; by MPS11 Publication1
Modified residuei91Phosphothreonine; by CDK1 and MPS11 Publication1
Modified residuei115Phosphoserine; by CDK11 Publication1
Modified residuei129Phosphothreonine; by CDK1 and MPS11 Publication1
Modified residuei134Phosphothreonine; by MPS11 Publication1
Modified residuei135Phosphoserine; by MPS11 Publication1
Modified residuei139Phosphothreonine; by CDK1 and MPS11 Publication1
Modified residuei153Phosphoserine; by MPS11 Publication1
Modified residuei174Phosphothreonine; by MPS11 Publication1
Modified residuei177Phosphoserine; by CDK11 Publication1
Modified residuei191Phosphothreonine; by CDK11 Publication1
Modified residuei192Phosphoserine; by CDK11 Publication1
Modified residuei268Phosphoserine; by CDK11 Publication1

Post-translational modificationi

Phosphorylation of the C-ter by MPS1 kinase regulates interaction with the outer kinetochore Ndc80 complex.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP43618
PRIDEiP43618

PTM databases

iPTMnetiP43618

Interactioni

Subunit structurei

Component of the inner kinetochore constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:14633972, PubMed:22561346). Interacts with outer kinetochore MIND complex subunit NNF1 (PubMed:14633972). Interacts with outer kinetochore Ndc80 complex subunits SPC24 and SPC25 (PubMed:22561346, PubMed:23334295).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31204, 122 interactors
DIPiDIP-5186N
IntActiP43618, 6 interactors
MINTiP43618
STRINGi4932.YFR046C

Structurei

Secondary structure

1361
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi63 – 78Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GEQX-ray2.01E/F60-84[»]
ProteinModelPortaliP43618
SMRiP43618
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 79Required for interaction with SPC24-SPC251 PublicationAdd BLAST15

Sequence similaritiesi

Belongs to the CENP-T/CNN1 family.Curated

Phylogenomic databases

InParanoidiP43618
OMAiRATNDDI
OrthoDBiEOG092C55JX

Sequencei

Sequence statusi: Complete.

P43618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPRKAAGN NENTEVSEIR TPFRERALEE QRLKDEVLIR NTPGYRKLLS
60 70 80 90 100
ASTKSHDILN KDPNEVRSFL QDLSQVLARK SQGNDTTTNK TQARNLIDEL
110 120 130 140 150
AYEESQPEEN ELLRSRSEKL TDNNIGNETQ PDYTSLSQTV FAKLQERDKG
160 170 180 190 200
LKSRKIDPII IQDVPTTGHE DELTVHSPDK ANSISMEVLR TSPSIGMDQV
210 220 230 240 250
DEPPVRDPVP ISITQQEEPL SEDLPSDDKE ETEEAENEDY SFENTSDENL
260 270 280 290 300
DDIGNDPIRL NVPAVRRSSI KPLQIMDLKH LTRQFLNENR IILPKQTWST
310 320 330 340 350
IQEESLNIMD FLKQKIGTLQ KQELVDSFID MGIINNVDDM FELAHELLPL
360
ELQSRIESYL F
Length:361
Mass (Da):41,313
Last modified:November 1, 1995 - v1
Checksum:i48CEEC087A056D42
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8A → T in AAT92741 (PubMed:7670463).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09285.1
AY692722 Genomic DNA Translation: AAT92741.1
BK006940 Genomic DNA Translation: DAA12489.1
PIRiS56301
RefSeqiNP_116704.1, NM_001180011.1

Genome annotation databases

EnsemblFungiiBAA09285; BAA09285; BAA09285
YFR046C; YFR046C; YFR046C
GeneIDi850607
KEGGisce:YFR046C

Similar proteinsi

Entry informationi

Entry nameiCENPT_YEAST
AccessioniPrimary (citable) accession number: P43618
Secondary accession number(s): D6VTS9, Q6B2K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health