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P43614 (KEG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,6-glucan synthesis-associated protein KEG1
Alternative name(s):
KRE6-binding ER protein responsible for glucan synthesis protein 1
Gene names
Name:KEG1
Ordered Locus Names:YFR042W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of (1->6)-beta-D-glucan polymers of the cell wall. Required for viability. Involved in maintaining chromosome stability. Ref.1 Ref.6

Subunit structure

Interacts with KRE6. Ref.1

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Beta-1,6-glucan synthesis-associated protein KEG1
PRO_0000202695

Regions

Topological domain1 – 1515Lumenal Potential
Transmembrane16 – 3621Helical; Potential
Topological domain37 – 448Cytoplasmic Potential
Transmembrane45 – 6521Helical; Potential
Topological domain66 – 8217Lumenal Potential
Transmembrane83 – 10321Helical; Potential
Topological domain104 – 14542Cytoplasmic Potential
Transmembrane146 – 16621Helical; Potential
Topological domain167 – 1737Lumenal Potential
Transmembrane174 – 19421Helical; Potential
Topological domain195 – 2006Cytoplasmic Potential

Natural variations

Natural variant31G → V in strain: KA31a. Ref.1
Natural variant551Y → C in strain: KA31a. Ref.1
Natural variant841N → D in strain: KA31a. Ref.1

Experimental info

Mutagenesis1241H → L in KEG1-1; temperature-sensitive. Increased sensitivity to chitin-binding dye Calcofluor white and zymolyase. Shows K1 killer toxin resistance, and reduced content of the cell wall beta-1,6-glucan. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43614 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E471CE48A72C2A61

FASTA20023,555
        10         20         30         40         50         60 
MAGIKLTHKL YQYYQLATSF LYAALLIRWL ILMPLVGSRF LPGGIHEFLI YLMFYSSIME 

        70         80         90        100        110        120 
VIWLLRFHGF KYGLLSRTFL KDLNFIYLVS VIHFYDDYEH ALILKNASYS SFIISLSLSQ 

       130        140        150        160        170        180 
AYCHWCKLFK RKGVKERTLV WKVNTFVTLP ILYLSEFALL LLNIQVKNYH STPTLDIINR 

       190        200 
VVLLAYFPVL LTAYKKLLTK 

« Hide

References

« Hide 'large scale' references
[1]"KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae."
Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K.
J. Biol. Chem. 282:34315-34324(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, INTERACTION WITH KRE6, SUBCELLULAR LOCATION, VARIANTS VAL-3; CYS-55 AND ASP-84, MUTAGENESIS OF HIS-124.
Strain: KA31a.
[2]"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae."
Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y.
Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[6]"Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae."
Ben-Aroya S., Coombes C., Kwok T., O'Donnell K.A., Boeke J.D., Hieter P.
Mol. Cell 30:248-258(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB303676 Genomic DNA. Translation: BAF62429.1.
D50617 Genomic DNA. Translation: BAA09281.1.
BK006940 Genomic DNA. Translation: DAA12485.1.
PIRS56297.
RefSeqNP_116700.1. NM_001180007.1.

3D structure databases

ProteinModelPortalP43614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31200. 14 interactions.
DIPDIP-1849N.
IntActP43614. 5 interactions.
MINTMINT-394790.
STRING4932.YFR042W.

Proteomic databases

PaxDbP43614.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR042W; YFR042W; YFR042W.
GeneID850603.
KEGGsce:YFR042W.

Organism-specific databases

CYGDYFR042w.
SGDS000001938. KEG1.

Phylogenomic databases

eggNOGNOG40874.
HOGENOMHOG000113218.
OMAYDDYEHA.
OrthoDBEOG7V4B9K.

Enzyme and pathway databases

BioCycYEAST:G3O-30489-MONOMER.

Gene expression databases

GenevestigatorP43614.

Family and domain databases

ProtoNetSearch...

Other

NextBio966475.

Entry information

Entry nameKEG1_YEAST
AccessionPrimary (citable) accession number: P43614
Secondary accession number(s): A5A8M3, D6VTS5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD