P43614 (KEG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,6-glucan synthesis-associated protein KEG1

Alternative name(s):
KRE6-binding ER protein responsible for glucan synthesis protein 1

Gene names

Name:	KEG1
Ordered Locus Names:	YFR042W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of (1->6)-beta-D-glucan polymers of the cell wall. Required for viability. Involved in maintaining chromosome stability. Ref.1 Ref.6
Subunit structure	Interacts with KRE6. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	(1->6)-beta-D-glucan biosynthetic process Inferred from mutant phenotype Ref.1. Source: SGD chromosome organization Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	integral to endoplasmic reticulum membrane Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Beta-1,6-glucan synthesis-associated protein KEG1		PRO_0000202695
Regions
	Topological domain	1 – 15	15	Lumenal Potential
	Transmembrane	16 – 36	21	Helical; Potential
	Topological domain	37 – 44	8	Cytoplasmic Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Topological domain	66 – 82	17	Lumenal Potential
	Transmembrane	83 – 103	21	Helical; Potential
	Topological domain	104 – 145	42	Cytoplasmic Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Topological domain	167 – 173	7	Lumenal Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Topological domain	195 – 200	6	Cytoplasmic Potential
Natural variations
	Natural variant	3	1	G → V in strain: KA31a. Ref.1
	Natural variant	55	1	Y → C in strain: KA31a. Ref.1
	Natural variant	84	1	N → D in strain: KA31a. Ref.1
Experimental info
	Mutagenesis	124	1	H → L in KEG1-1; temperature-sensitive. Increased sensitivity to chitin-binding dye Calcofluor white and zymolyase. Shows K1 killer toxin resistance, and reduced content of the cell wall beta-1,6-glucan. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43614 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E471CE48A72C2A61	FASTA	200	23,555
	10 20 30 40 50 60 MAGIKLTHKL YQYYQLATSF LYAALLIRWL ILMPLVGSRF LPGGIHEFLI YLMFYSSIME 70 80 90 100 110 120 VIWLLRFHGF KYGLLSRTFL KDLNFIYLVS VIHFYDDYEH ALILKNASYS SFIISLSLSQ 130 140 150 160 170 180 AYCHWCKLFK RKGVKERTLV WKVNTFVTLP ILYLSEFALL LLNIQVKNYH STPTLDIINR 190 200 VVLLAYFPVL LTAYKKLLTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae." Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K. J. Biol. Chem. 282:34315-34324(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, INTERACTION WITH KRE6, SUBCELLULAR LOCATION, VARIANTS VAL-3; CYS-55 AND ASP-84, MUTAGENESIS OF HIS-124. Strain: KA31a.
[2]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae." Ben-Aroya S., Coombes C., Kwok T., O'Donnell K.A., Boeke J.D., Hieter P. Mol. Cell 30:248-258(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB303676 Genomic DNA. Translation: BAF62429.1. D50617 Genomic DNA. Translation: BAA09281.1. BK006940 Genomic DNA. Translation: DAA12485.1.
PIR	S56297.
RefSeq	NP_116700.1. NM_001180007.1.
3D structure databases
ProteinModelPortal	P43614.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1849N.
IntAct	P43614. 2 interactions.
MINT	MINT-394790.
STRING	4932.YFR042W.
Proteomic databases
PaxDb	P43614.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR042W; YFR042W; YFR042W.
GeneID	850603.
KEGG	sce:YFR042W.
Organism-specific databases
CYGD	YFR042w.
SGD	S000001938. KEG1.
Phylogenomic databases
eggNOG	NOG40874.
HOGENOM	HOG000113218.
OMA	YDDYEHA.
OrthoDB	EOG4TMVBM.
Gene expression databases
Genevestigator	P43614.
GermOnline	YFR042W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	966475.

Entry information

Entry name

KEG1_YEAST

Accession

Primary (citable) accession number: P43614
Secondary accession number(s): A5A8M3, D6VTS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program