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P43614

- KEG1_YEAST

UniProt

P43614 - KEG1_YEAST

Protein

Beta-1,6-glucan synthesis-associated protein KEG1

Gene

KEG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of (1->6)-beta-D-glucan polymers of the cell wall. Required for viability. Involved in maintaining chromosome stability.2 Publications

    GO - Biological processi

    1. (1->6)-beta-D-glucan biosynthetic process Source: SGD
    2. chromosome organization Source: SGD

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30489-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,6-glucan synthesis-associated protein KEG1
    Alternative name(s):
    KRE6-binding ER protein responsible for glucan synthesis protein 1
    Gene namesi
    Name:KEG1
    Ordered Locus Names:YFR042W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR042w.
    SGDiS000001938. KEG1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi124 – 1241H → L in KEG1-1; temperature-sensitive. Increased sensitivity to chitin-binding dye Calcofluor white and zymolyase. Shows K1 killer toxin resistance, and reduced content of the cell wall beta-1,6-glucan. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Beta-1,6-glucan synthesis-associated protein KEG1PRO_0000202695Add
    BLAST

    Proteomic databases

    PaxDbiP43614.

    Expressioni

    Gene expression databases

    GenevestigatoriP43614.

    Interactioni

    Subunit structurei

    Interacts with KRE6.1 Publication

    Protein-protein interaction databases

    BioGridi31200. 14 interactions.
    DIPiDIP-1849N.
    IntActiP43614. 5 interactions.
    MINTiMINT-394790.
    STRINGi4932.YFR042W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43614.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515LumenalSequence AnalysisAdd
    BLAST
    Topological domaini37 – 448CytoplasmicSequence Analysis
    Topological domaini66 – 8217LumenalSequence AnalysisAdd
    BLAST
    Topological domaini104 – 14542CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini167 – 1737LumenalSequence Analysis
    Topological domaini195 – 2006CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei45 – 6521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei83 – 10321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei174 – 19421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40874.
    HOGENOMiHOG000113218.
    OMAiYDDYEHA.
    OrthoDBiEOG7V4B9K.

    Sequencei

    Sequence statusi: Complete.

    P43614-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGIKLTHKL YQYYQLATSF LYAALLIRWL ILMPLVGSRF LPGGIHEFLI    50
    YLMFYSSIME VIWLLRFHGF KYGLLSRTFL KDLNFIYLVS VIHFYDDYEH 100
    ALILKNASYS SFIISLSLSQ AYCHWCKLFK RKGVKERTLV WKVNTFVTLP 150
    ILYLSEFALL LLNIQVKNYH STPTLDIINR VVLLAYFPVL LTAYKKLLTK 200
    Length:200
    Mass (Da):23,555
    Last modified:November 1, 1995 - v1
    Checksum:iE471CE48A72C2A61
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti3 – 31G → V in strain: KA31a. 1 Publication
    Natural varianti55 – 551Y → C in strain: KA31a. 1 Publication
    Natural varianti84 – 841N → D in strain: KA31a. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB303676 Genomic DNA. Translation: BAF62429.1.
    D50617 Genomic DNA. Translation: BAA09281.1.
    BK006940 Genomic DNA. Translation: DAA12485.1.
    PIRiS56297.
    RefSeqiNP_116700.1. NM_001180007.1.

    Genome annotation databases

    EnsemblFungiiYFR042W; YFR042W; YFR042W.
    GeneIDi850603.
    KEGGisce:YFR042W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB303676 Genomic DNA. Translation: BAF62429.1 .
    D50617 Genomic DNA. Translation: BAA09281.1 .
    BK006940 Genomic DNA. Translation: DAA12485.1 .
    PIRi S56297.
    RefSeqi NP_116700.1. NM_001180007.1.

    3D structure databases

    ProteinModelPortali P43614.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31200. 14 interactions.
    DIPi DIP-1849N.
    IntActi P43614. 5 interactions.
    MINTi MINT-394790.
    STRINGi 4932.YFR042W.

    Proteomic databases

    PaxDbi P43614.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR042W ; YFR042W ; YFR042W .
    GeneIDi 850603.
    KEGGi sce:YFR042W.

    Organism-specific databases

    CYGDi YFR042w.
    SGDi S000001938. KEG1.

    Phylogenomic databases

    eggNOGi NOG40874.
    HOGENOMi HOG000113218.
    OMAi YDDYEHA.
    OrthoDBi EOG7V4B9K.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30489-MONOMER.

    Miscellaneous databases

    NextBioi 966475.

    Gene expression databases

    Genevestigatori P43614.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae."
      Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K.
      J. Biol. Chem. 282:34315-34324(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, INTERACTION WITH KRE6, SUBCELLULAR LOCATION, VARIANTS VAL-3; CYS-55 AND ASP-84, MUTAGENESIS OF HIS-124.
      Strain: KA31a.
    2. "Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y.
      Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae."
      Ben-Aroya S., Coombes C., Kwok T., O'Donnell K.A., Boeke J.D., Hieter P.
      Mol. Cell 30:248-258(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiKEG1_YEAST
    AccessioniPrimary (citable) accession number: P43614
    Secondary accession number(s): A5A8M3, D6VTS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3