ID   ERJ5_YEAST              Reviewed;         295 AA.
AC   P43613; D6VTS4;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 174.
DE   RecName: Full=ER-localized J domain-containing protein 5;
DE   Flags: Precursor;
GN   Name=ERJ5; OrderedLocusNames=YFR041C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   PubMed=8686379;
RX   DOI=10.1002/(sici)1097-0061(199602)12:2<149::aid-yea893>3.0.co;2-g;
RA   Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M.,
RA   Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y.;
RT   "Analysis of a 36.2 kb DNA sequence including the right telomere of
RT   chromosome VI from Saccharomyces cerevisiae.";
RL   Yeast 12:149-167(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7670463; DOI=10.1038/ng0795-261;
RA   Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA   Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA   Eki T.;
RT   "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT   cerevisiae.";
RL   Nat. Genet. 10:261-268(1995).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [6]
RP   SUBCELLULAR LOCATION, SIGNAL SEQUENCE CLEAVAGE, TOPOLOGY, AND FUNCTION.
RX   PubMed=17157937; DOI=10.1016/j.bbamcr.2006.10.011;
RA   Carla Fama M., Raden D., Zacchi N., Lemos D.R., Robinson A.S.,
RA   Silberstein S.;
RT   "The Saccharomyces cerevisiae YFR041C/ERJ5 gene encoding a type I membrane
RT   protein with a J domain is required to preserve the folding capacity of the
RT   endoplasmic reticulum.";
RL   Biochim. Biophys. Acta 1773:232-242(2007).
CC   -!- FUNCTION: DnaJ-like chaperone required for the folding capacity of the
CC       endoplasmic reticulum. {ECO:0000269|PubMed:17157937}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:17157937}; Single-pass
CC       type I membrane protein {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:17157937}.
CC   -!- SIMILARITY: Belongs to the DnaJ family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D50617; BAA09280.1; -; Genomic_DNA.
DR   EMBL; BK006940; DAA12484.1; -; Genomic_DNA.
DR   PIR; S56296; S56296.
DR   RefSeq; NP_116699.3; NM_001180006.3.
DR   AlphaFoldDB; P43613; -.
DR   SMR; P43613; -.
DR   BioGRID; 31199; 45.
DR   IntAct; P43613; 2.
DR   MINT; P43613; -.
DR   STRING; 4932.YFR041C; -.
DR   iPTMnet; P43613; -.
DR   MaxQB; P43613; -.
DR   PaxDb; 4932-YFR041C; -.
DR   PeptideAtlas; P43613; -.
DR   EnsemblFungi; YFR041C_mRNA; YFR041C; YFR041C.
DR   GeneID; 850602; -.
DR   KEGG; sce:YFR041C; -.
DR   AGR; SGD:S000001937; -.
DR   SGD; S000001937; ERJ5.
DR   VEuPathDB; FungiDB:YFR041C; -.
DR   eggNOG; KOG0724; Eukaryota.
DR   HOGENOM; CLU_037236_2_0_1; -.
DR   InParanoid; P43613; -.
DR   OMA; RRYDYFY; -.
DR   OrthoDB; 1327728at2759; -.
DR   BioCyc; YEAST:G3O-30488-MONOMER; -.
DR   BioGRID-ORCS; 850602; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P43613; -.
DR   Proteomes; UP000002311; Chromosome VI.
DR   RNAct; P43613; Protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0006457; P:protein folding; IMP:SGD.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; DnaJ domain; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR044632; DNAJC25-like.
DR   InterPro; IPR036869; J_dom_sf.
DR   PANTHER; PTHR44176; DNAJ HOMOLOG SUBFAMILY C MEMBER 25; 1.
DR   PANTHER; PTHR44176:SF1; DNAJ HOMOLOG SUBFAMILY C MEMBER 25; 1.
DR   Pfam; PF00226; DnaJ; 1.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; Chaperone J-domain; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Endoplasmic reticulum; Membrane; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..20
FT   CHAIN           21..295
FT                   /note="ER-localized J domain-containing protein 5"
FT                   /id="PRO_0000007272"
FT   TOPO_DOM        21..130
FT                   /note="Lumenal"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        152..295
FT                   /note="Cytoplasmic"
FT   DOMAIN          42..110
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          259..287
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   295 AA;  34191 MW;  4541BF6C353DB8AC CRC64;
     MNGYWKPALV VLGLVSLSYA FTTIETEIFQ LQNEISTKYG PDMNFYKFLK LPKLQNSSTK
     EITKNLRKLS KKYHPDKNPK YRKLYERLNL ATQILSNSSN RKIYDYYLQN GFPNYDFHKG
     GFYFSRMKPK TWFLLAFIWI VVNIGQYIIS IIQYRSQRSR IENFISQCKQ QDDTNGLGVK
     QLTFKQHEKD EGKSLVVRFS DVYVVEPDGS ETLISPDTLD KPSVKNCLFW RIPASVWNMT
     FGKSVGSAGK EEIITDSKKY DGNQTKKGNK VKKGSAKKGQ KKMELPNGKV IYSRK
//