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Protein

SIT4-associating protein SAP155

Gene

SAP155

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positive regulator of protein phosphatase SIT4. Involved in directing expression of TOR-repressed genes and in dephosphorylation of NPR1 in response to nutrient starvation. Negatively modulates K+ efflux of the cell by the Na+-K+/H+ antiporter NHA1.2 Publications

Miscellaneous

Present with 5960 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • G1/S transition of mitotic cell cycle Source: SGD

Keywordsi

Biological processCell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-30487-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
SIT4-associating protein SAP155
Gene namesi
Name:SAP155
Ordered Locus Names:YFR040W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR040W
SGDiS000001936 SAP155

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975611 – 1002SIT4-associating protein SAP155Add BLAST1002

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei255PhosphoserineCombined sources1
Modified residuei613PhosphothreonineCombined sources1
Modified residuei618PhosphothreonineCombined sources1

Post-translational modificationi

Hyperphosphorylated in the absence of SIT4.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43612
PaxDbiP43612
PRIDEiP43612

PTM databases

iPTMnetiP43612

Interactioni

Subunit structurei

Associates with the SIT4 protein phosphatase catalytic subunit in a cell-cycle-dependent manner.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31198, 340 interactors
DIPiDIP-5852N
IntActiP43612, 45 interactors
MINTiP43612
STRINGi4932.YFR040W

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi938 – 997Asn-richAdd BLAST60

Sequence similaritiesi

Belongs to the SAPS family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009899
HOGENOMiHOG000142084
InParanoidiP43612
KOiK15457
OMAiDYDLNCG
OrthoDBiEOG092C0HJS

Family and domain databases

InterProiView protein in InterPro
IPR007587 SAPS
PANTHERiPTHR12634 PTHR12634, 2 hits
PfamiView protein in Pfam
PF04499 SAPS, 1 hit

Sequencei

Sequence statusi: Complete.

P43612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFWPFGQNL NHSNINKILD EYFHVLHELE RINPSVGKAI PAIFNNVQER
60 70 80 90 100
GTSDSLDSIP EEYSHGDEVK TARGDQKSRF EKDDQQERYE KEEEERSMNS
110 120 130 140 150
SESSTTSFSS GSTSKTDLDE EDISNATAPM MVTTKNLDNS FIERMLVETE
160 170 180 190 200
LLNELSRQNK TLLDFICFGF FFDKKTNKKV NNMEYLVDQL MECISKIKTA
210 220 230 240 250
TTVDLNNLID YQEQQQLDDS SQEDVYVESD TEQEEEKEDD NNSNNKKRRK
260 270 280 290 300
RGSSSFGNDD INNNDDDDDA NEDDESAYLT KATIISEIFS LDIWLISESL
310 320 330 340 350
VKNQSYLNKI WSIINQPNFN SENSPLVPIF LKINQNLLLT RQDQYLNFIR
360 370 380 390 400
TERSFVDDML KHVDISLLMD FFLKIISTDK IESPTGIIEL VYDQNLISKC
410 420 430 440 450
LSFLNNKESP ADIQACVGDF LKALIAISAN APLDDISIGP NSLTRQLASP
460 470 480 490 500
ESIAKLVDIM INQRGAALNT TVSIVIELIR KNNSDYDQVN LLTTTIKTHP
510 520 530 540 550
PSNRDPIYLG YLLRKFSNHL SDFFQIILDI ENDANIPLHE NQLHEKFKPL
560 570 580 590 600
GFERFKVVEL IAELLHCSNM GLMNSKRAER IARRRDKVRS QLSHHLQDAL
610 620 630 640 650
NDLSIEEKEQ LKTKHSPTRD TDHDLKNNNG KIDNDNNDND DESDYGDEID
660 670 680 690 700
ESFEIPYINM KQNIKLRTDP TVGDLFKIKL YDTRIVSKIM ELFLTHPWNN
710 720 730 740 750
FWHNVIFDII QQIFNGRMDF SYNSFLVLSL FNLKSSYQFM TDIVISDEKG
760 770 780 790 800
TDVSRFSPVI RDPNFDFKIT TDFILRGYQD SYKFYELRKM NLGYMGHIVL
810 820 830 840 850
IAEEVVKFSK LYKVELISPD IQVILQTEEW QYYSEEVLNE TRMMYSKILG
860 870 880 890 900
GGSYIDDGNG NIIPQLPDNT TVLTPNGDAS NNNEILDSDT GSSNGTSGGG
910 920 930 940 950
QLINVESLEE QLSLSTESDL HNKLREMLIN RAQEDVDNKN TENGVFILGP
960 970 980 990 1000
PEDKNSNSNI NNTNHNSNNS NNNDNNDNND NDNDNTRNYN EDADNDNDYD

HE
Length:1,002
Mass (Da):115,002
Last modified:September 21, 2011 - v4
Checksum:i50CDC17130333022
GO

Sequence cautioni

The sequence BAA09279 differs from that shown. Reason: Frameshift at position 74.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti663N → T (PubMed:8686379).Curated1
Sequence conflicti663N → T in BAA09279 (PubMed:7670463).Curated1
Sequence conflicti668T → G in CAC42243 (PubMed:11779790).Curated1
Sequence conflicti674 – 690DLFKI…VSKIM → TYSKSNYMIRDCFQNN AA sequence (PubMed:8649382).CuratedAdd BLAST17
Sequence conflicti815 – 824ELISPDIQVI → DYISRYSSN in AAC49303 (PubMed:8649382).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50560 Genomic DNA Translation: AAC49303.1
AJ318331 Genomic DNA Translation: CAC42243.1
D50617 Genomic DNA Translation: BAA09279.1 Frameshift.
BK006940 Genomic DNA Translation: DAA12483.2
RefSeqiNP_116698.3, NM_001180005.2

Genome annotation databases

EnsemblFungiiBAA09279; BAA09279; BAA09279
YFR040W; YFR040W; YFR040W
GeneIDi850601
KEGGisce:YFR040W

Similar proteinsi

Entry informationi

Entry nameiSA155_YEAST
AccessioniPrimary (citable) accession number: P43612
Secondary accession number(s): D6VTS3, Q96VG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 21, 2011
Last modified: May 23, 2018
This is version 156 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names
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