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P43612 (SA155_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SIT4-associating protein SAP155
Gene names
Name:SAP155
Ordered Locus Names:YFR040W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1002 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulator of protein phosphatase SIT4. Involved in directing expression of TOR-repressed genes and in dephosphorylation of NPR1 in response to nutrient starvation. Negatively modulates K+ efflux of the cell by the Na+-K+/H+ antiporter NHA1. Ref.8 Ref.9

Subunit structure

Associates with the SIT4 protein phosphatase catalytic subunit in a cell-cycle-dependent manner.

Subcellular location

Cytoplasm Ref.6.

Post-translational modification

Hyperphosphorylated in the absence of SIT4. Ref.1

Miscellaneous

Present with 5960 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SAPS family.

Sequence caution

The sequence BAA09279.1 differs from that shown. Reason: Frameshift at position 74.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SIT4P206048EBI-16370,EBI-13707

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10021002SIT4-associating protein SAP155
PRO_0000097561

Regions

Compositional bias938 – 99760Asn-rich

Amino acid modifications

Modified residue581Phosphoserine Ref.11
Modified residue2551Phosphoserine Ref.12
Modified residue6131Phosphothreonine Ref.10
Modified residue6181Phosphothreonine Ref.10

Experimental info

Sequence conflict6631N → T Ref.3
Sequence conflict6631N → T in BAA09279. Ref.4
Sequence conflict6681T → G in CAC42243. Ref.2
Sequence conflict674 – 69017DLFKI…VSKIM → TYSKSNYMIRDCFQNN AA sequence Ref.1
Sequence conflict815 – 82410ELISPDIQVI → DYISRYSSN in AAC49303. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43612 [UniParc].

Last modified September 21, 2011. Version 4.
Checksum: 50CDC17130333022

FASTA1,002115,002
        10         20         30         40         50         60 
MSFWPFGQNL NHSNINKILD EYFHVLHELE RINPSVGKAI PAIFNNVQER GTSDSLDSIP 

        70         80         90        100        110        120 
EEYSHGDEVK TARGDQKSRF EKDDQQERYE KEEEERSMNS SESSTTSFSS GSTSKTDLDE 

       130        140        150        160        170        180 
EDISNATAPM MVTTKNLDNS FIERMLVETE LLNELSRQNK TLLDFICFGF FFDKKTNKKV 

       190        200        210        220        230        240 
NNMEYLVDQL MECISKIKTA TTVDLNNLID YQEQQQLDDS SQEDVYVESD TEQEEEKEDD 

       250        260        270        280        290        300 
NNSNNKKRRK RGSSSFGNDD INNNDDDDDA NEDDESAYLT KATIISEIFS LDIWLISESL 

       310        320        330        340        350        360 
VKNQSYLNKI WSIINQPNFN SENSPLVPIF LKINQNLLLT RQDQYLNFIR TERSFVDDML 

       370        380        390        400        410        420 
KHVDISLLMD FFLKIISTDK IESPTGIIEL VYDQNLISKC LSFLNNKESP ADIQACVGDF 

       430        440        450        460        470        480 
LKALIAISAN APLDDISIGP NSLTRQLASP ESIAKLVDIM INQRGAALNT TVSIVIELIR 

       490        500        510        520        530        540 
KNNSDYDQVN LLTTTIKTHP PSNRDPIYLG YLLRKFSNHL SDFFQIILDI ENDANIPLHE 

       550        560        570        580        590        600 
NQLHEKFKPL GFERFKVVEL IAELLHCSNM GLMNSKRAER IARRRDKVRS QLSHHLQDAL 

       610        620        630        640        650        660 
NDLSIEEKEQ LKTKHSPTRD TDHDLKNNNG KIDNDNNDND DESDYGDEID ESFEIPYINM 

       670        680        690        700        710        720 
KQNIKLRTDP TVGDLFKIKL YDTRIVSKIM ELFLTHPWNN FWHNVIFDII QQIFNGRMDF 

       730        740        750        760        770        780 
SYNSFLVLSL FNLKSSYQFM TDIVISDEKG TDVSRFSPVI RDPNFDFKIT TDFILRGYQD 

       790        800        810        820        830        840 
SYKFYELRKM NLGYMGHIVL IAEEVVKFSK LYKVELISPD IQVILQTEEW QYYSEEVLNE 

       850        860        870        880        890        900 
TRMMYSKILG GGSYIDDGNG NIIPQLPDNT TVLTPNGDAS NNNEILDSDT GSSNGTSGGG 

       910        920        930        940        950        960 
QLINVESLEE QLSLSTESDL HNKLREMLIN RAQEDVDNKN TENGVFILGP PEDKNSNSNI 

       970        980        990       1000 
NNTNHNSNNS NNNDNNDNND NDNDNTRNYN EDADNDNDYD HE 

« Hide

References

« Hide 'large scale' references
[1]"The SAPs, a new family of proteins, associate and function positively with the SIT4 phosphatase."
Luke M.M., della Seta F., di Como C.J., Sugimoto H., Kobayashi R., Arndt K.T.
Mol. Cell. Biol. 16:2744-2755(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, INTERACTION WITH SIT4, PHOSPHORYLATION.
[2]"Sit4p protein phosphatase is required for sensitivity of Saccharomyces cerevisiae to Kluyveromyces lactis zymocin."
Jablonowski D., Butler A.R., Fichtner L., Gardiner D., Schaffrath R., Stark M.J.R.
Genetics 159:1479-1489(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF FRAMESHIFT.
[3]"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae."
Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y.
Yeast 12:149-167(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 663.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"TOR controls transcriptional and translational programs via Sap-Sit4 protein phosphatase signaling effectors."
Rohde J.R., Campbell S., Zurita-Martinez S.A., Cutler N.S., Ashe M., Cardenas M.E.
Mol. Cell. Biol. 24:8332-8341(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Ability of Sit4p to promote K+ efflux via Nha1p is modulated by Sap155p and Sap185p."
Manlandro C.M.A., Haydon D.H., Rosenwald A.G.
Eukaryot. Cell 4:1041-1049(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-613 AND THR-618, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U50560 Genomic DNA. Translation: AAC49303.1.
AJ318331 Genomic DNA. Translation: CAC42243.1.
D50617 Genomic DNA. Translation: BAA09279.1. Frameshift.
BK006940 Genomic DNA. Translation: DAA12483.2.
RefSeqNP_116698.3. NM_001180005.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31198. 150 interactions.
DIPDIP-5852N.
IntActP43612. 32 interactions.
MINTMINT-632146.
STRING4932.YFR040W.

Proteomic databases

MaxQBP43612.
PaxDbP43612.
PeptideAtlasP43612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR040W; YFR040W; YFR040W.
GeneID850601.
KEGGsce:YFR040W.

Organism-specific databases

CYGDYFR040w.
SGDS000001936. SAP155.

Phylogenomic databases

eggNOGNOG301298.
GeneTreeENSGT00390000009899.
HOGENOMHOG000142084.
KOK15457.
OMANNFWHNV.
OrthoDBEOG7647CP.

Enzyme and pathway databases

BioCycYEAST:G3O-30487-MONOMER.

Gene expression databases

GenevestigatorP43612.

Family and domain databases

InterProIPR007587. SAPS.
[Graphical view]
PANTHERPTHR12634. PTHR12634. 1 hit.
PfamPF04499. SAPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966469.

Entry information

Entry nameSA155_YEAST
AccessionPrimary (citable) accession number: P43612
Secondary accession number(s): D6VTS3, Q96VG4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families