P43610 (IRC5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized ATP-dependent helicase IRC5
EC=3.6.4.-

Alternative name(s):
Increased recombination centers protein 5

Gene names

Name:	IRC5
Ordered Locus Names:	YFR038W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is probably involved in a pathway contributing to genomic integrity.
Subcellular location	Nucleus Potential.
Disruption phenotype	Displays increased levels of spontaneous RAD52 foci in proliferating diploid cells. Ref.6
Sequence similarities	Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence BAA09277.1 differs from that shown. Reason: Frameshift at position 769.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitotic recombination Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 853	853	Uncharacterized ATP-dependent helicase IRC5		PRO_0000074385
Regions
	Domain	234 – 401	168	Helicase ATP-binding
	Domain	607 – 758	152	Helicase C-terminal
	Nucleotide binding	247 – 254	8	ATP Potential
	Coiled coil	548 – 580	33	Potential
	Motif	352 – 355	4	DEGH box
	Compositional bias	41 – 91	51	Asp-rich

Sequences

	Sequence		Length	Mass (Da)
	P43610 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 91EF0E200197FF28	FASTA	853	96,966
	10 20 30 40 50 60 MSRCSNAALM TVVEDAVGAR VAARTRNMSN GVNYREKEVN DLTADISDSD SDLDSEDNKH 70 80 90 100 110 120 GKGDNDTAPI WLQDDVHSDE DIQLDSEDDS DTEAVQAQVV DKLAKDTKSE QKSLDDELSE 130 140 150 160 170 180 MDTKTVSLKL KKLNEFVRQS QVYSSIIADT LLHRSNEVAN ANTKDNSNSD DEEHSSKKRK 190 200 210 220 230 240 TKKKSITDFF KKQKKNEDTT TQNGAPDDAA IKQPRLLKNC ILKPYQLEGL NWLITLYENG 250 260 270 280 290 300 LNGILADEMG LGKTVQSIAL LAFIYEMDTK GPFLVTAPLS TLDNWMNEFA KFAPDLPVLK 310 320 330 340 350 360 YYGTNGYKER SAKLKNFFKQ HGGTGIVITS YEIILRDTDL IMSQNWKFLI VDEGHRLKNI 370 380 390 400 410 420 NCRLIKELKK INTSNRLLLT GTPLQNNLAE LWSLLNFIMP DIFADFEIFN KWFDFDSLNL 430 440 450 460 470 480 GSGSNSEALN KLINDELQKN LISNLHTILK PFLLRRLKKV VLANILPPKR EYIINCPMTS 490 500 510 520 530 540 AQEKFYKAGL NGKLKKTMFK ELIKDFFTLN DEYIGHVSNR SIRDFINYKL SGNETSNTDN 550 560 570 580 590 600 KINPTLLQMD KLYKKNLQME ISNKKLQNMM MQLRQIIDST FLFYFPYLHP EDLTLETLLK 610 620 630 640 650 660 TSGKLQILQK LIPPLISEGH KVLIYSQFVN MLDLIEDWCD LNSFATFRID GSVNNETRKD 670 680 690 700 710 720 QLEKFNSSKD KHNIFLLSTR AAGLGINLVG ADTVVLFDSD WNPQVDLQAM DRCHRIGQES 730 740 750 760 770 780 PVIVYRLCCD NTIEHVILTR AANKRNLERM VIQMGKFNNL KKLALNEGSF LKANKAGVNV 790 800 810 820 830 840 TNKDLVQELS MLLMSDESNI GFENGGQKEN KATEGQLTDK EVEELTNRSL EAYKANRVVD 850 LPHVKLFETT SGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT. Strain: ATCC 204511 / S288c / AB972.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[6]	"Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination." Alvaro D., Lisby M., Rothstein R. PLoS Genet. 3:E228-E228(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09277.1. Frameshift. BK006940 Genomic DNA. Translation: DAA12481.1.
PIR	S56293.
RefSeq	NP_116696.2. NM_001180003.1.
3D structure databases
ProteinModelPortal	P43610.
SMR	P43610. Positions 213-487, 511-764.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5300N.
MINT	MINT-543614.
STRING	4932.YFR038W.
Proteomic databases
PaxDb	P43610.
PRIDE	P43610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR038W; YFR038W; YFR038W.
GeneID	850599.
KEGG	sce:YFR038W.
Organism-specific databases
CYGD	YFR038w.
SGD	S000001934. IRC5.
Phylogenomic databases
eggNOG	COG0553.
GeneTree	ENSGT00550000075106.
OMA	FEIFNKW.
OrthoDB	EOG40S3Q6.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30485-MONOMER.
Gene expression databases
Genevestigator	P43610.
GermOnline	YFR038W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966463.

Entry information

Entry name

IRC5_YEAST

Accession

Primary (citable) accession number: P43610
Secondary accession number(s): D6VTS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 20, 2005
Last modified:	May 29, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program