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Protein

Chromatin structure-remodeling complex protein RSC8

Gene

RSC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.7 Publications

Miscellaneous

Present with 3380 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri254 – 298ZZ-typeAdd BLAST45

GO - Molecular functioni

  • DNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: UniProtKB
  • covalent chromatin modification Source: UniProtKB-KW
  • double-strand break repair via nonhomologous end joining Source: SGD
  • nucleosome disassembly Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionChromatin regulator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex protein RSC8
Alternative name(s):
Remodel the structure of chromatin complex subunit 8
SWI3 homolog
Gene namesi
Name:RSC8
Synonyms:SWH3
Ordered Locus Names:YFR037C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR037C.
SGDiS000001933. RSC8.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  • RSC complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi347 – 351Missing : Loss of function. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001971141 – 557Chromatin structure-remodeling complex protein RSC8Add BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43609.
PRIDEiP43609.

PTM databases

iPTMnetiP43609.

Interactioni

Subunit structurei

Dimerizes via the C-terminal coiled coil. Interacts directly with HTL1, NPL6, RSC6 and the N-terminus of STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31195. 473 interactors.
DIPiDIP-1478N.
IntActiP43609. 93 interactors.
MINTiMINT-401637.

Structurei

3D structure databases

ProteinModelPortaliP43609.
SMRiP43609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 177SWIRMPROSITE-ProRule annotationAdd BLAST98
Domaini310 – 362SANTPROSITE-ProRule annotationAdd BLAST53

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 77Sequence analysisAdd BLAST40
Coiled coili462 – 494Sequence analysisAdd BLAST33

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri254 – 298ZZ-typeAdd BLAST45

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000246692.
InParanoidiP43609.
KOiK11762.
OMAiLCSRCFQ.
OrthoDBiEOG092C5VLW.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR032451. SMARCC_C.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
IPR000433. Znf_ZZ.
PfamiView protein in Pfam
PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF16495. SWIRM-assoc_1. 1 hit.
PF00569. ZZ. 1 hit.
SMARTiView protein in SMART
SM00717. SANT. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiView protein in PROSITE
PS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.

Sequencei

Sequence statusi: Complete.

P43609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE
60 70 80 90 100
VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWFD ISKIHEIEKR
110 120 130 140 150
SNPDFFNDSS RFKTPKAYKD TRNFIINTYR LSPYEYLTIT AVRRNVAMDV
160 170 180 190 200
ASIVKIHAFL EKWGLINYQI DPRTKPSLIG PSFTGHFQVV LDTPQGLKPF
210 220 230 240 250
LPENVIKQEV EGGDGAEPQV KKEFPVNLTI KKNVYDSAQD FNALQDESRN
260 270 280 290 300
SRQIHKVYIC HTCGNESINV RYHNLRARDT NLCSRCFQEG HFGANFQSSD
310 320 330 340 350
FIRLENNGNS VKKNWSDQEM LLLLEGIEMY EDQWEKIADH VGGHKRVEDC
360 370 380 390 400
IEKFLSLPIE DNYIREVVGS TLNGKGGDSR DGSVSGSKLM ECVNDAVQTL
410 420 430 440 450
LQGDDKLGKV SDKSREISEK YIEESQAIIQ ELVKLTMEKL ESKFTKLCDL
460 470 480 490 500
ETQLEMEKLK YVKESEKMLN DRLSLSKQIL DLNKSLEELN VSKKLVLISE
510 520 530 540 550
QVDSGIQLVE KDQEGDDEDG NTATGHGVKR VGKEGEEVGE GDSIAKLQPQ

VYKPWSL
Length:557
Mass (Da):63,168
Last modified:November 1, 1995 - v1
Checksum:iD3D3120397CF0058
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192D → G in AAT92860 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09276.1.
AY692841 Genomic DNA. Translation: AAT92860.1.
BK006940 Genomic DNA. Translation: DAA12480.1.
PIRiS56292.
RefSeqiNP_116695.3. NM_001180002.3.

Genome annotation databases

EnsemblFungiiBAA09276; BAA09276; BAA09276.
YFR037C; YFR037C; YFR037C.
GeneIDi850598.
KEGGisce:YFR037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09276.1.
AY692841 Genomic DNA. Translation: AAT92860.1.
BK006940 Genomic DNA. Translation: DAA12480.1.
PIRiS56292.
RefSeqiNP_116695.3. NM_001180002.3.

3D structure databases

ProteinModelPortaliP43609.
SMRiP43609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31195. 473 interactors.
DIPiDIP-1478N.
IntActiP43609. 93 interactors.
MINTiMINT-401637.

PTM databases

iPTMnetiP43609.

Proteomic databases

MaxQBiP43609.
PRIDEiP43609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09276; BAA09276; BAA09276.
YFR037C; YFR037C; YFR037C.
GeneIDi850598.
KEGGisce:YFR037C.

Organism-specific databases

EuPathDBiFungiDB:YFR037C.
SGDiS000001933. RSC8.

Phylogenomic databases

HOGENOMiHOG000246692.
InParanoidiP43609.
KOiK11762.
OMAiLCSRCFQ.
OrthoDBiEOG092C5VLW.

Enzyme and pathway databases

BioCyciYEAST:G3O-30484-MONOMER.

Miscellaneous databases

PROiPR:P43609.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR032451. SMARCC_C.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
IPR000433. Znf_ZZ.
PfamiView protein in Pfam
PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF16495. SWIRM-assoc_1. 1 hit.
PF00569. ZZ. 1 hit.
SMARTiView protein in SMART
SM00717. SANT. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiView protein in PROSITE
PS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSC8_YEAST
AccessioniPrimary (citable) accession number: P43609
Secondary accession number(s): D6VTS0, Q6B289
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 12, 2017
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.