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P43609

- RSC8_YEAST

UniProt

P43609 - RSC8_YEAST

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Protein

Chromatin structure-remodeling complex protein RSC8

Gene

RSC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri254 – 29845ZZ-typeAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA binding Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP-dependent chromatin remodeling Source: UniProtKB
  3. double-strand break repair via nonhomologous end joining Source: SGD
  4. G1/S transition of mitotic cell cycle Source: SGD
  5. nucleosome disassembly Source: SGD
  6. regulation of transcription, DNA-templated Source: UniProtKB
  7. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex protein RSC8
Alternative name(s):
Remodel the structure of chromatin complex subunit 8
SWI3 homolog
Gene namesi
Name:RSC8
Synonyms:SWH3
Ordered Locus Names:YFR037C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR037c.
SGDiS000001933. RSC8.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation
Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  1. RSC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi347 – 3515Missing: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Chromatin structure-remodeling complex protein RSC8PRO_0000197114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei485 – 4851Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43609.
PaxDbiP43609.
PeptideAtlasiP43609.

Expressioni

Gene expression databases

GenevestigatoriP43609.

Interactioni

Subunit structurei

Dimerizes via the C-terminal coiled coil. Interacts directly with HTL1, NPL6, RSC6 and the N-terminus of STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HTL1Q9URQ53EBI-23005,EBI-8717
NPL6P328327EBI-23005,EBI-12202
RSC6P2563212EBI-23005,EBI-21941
RSC9Q031244EBI-23005,EBI-27977

Protein-protein interaction databases

BioGridi31195. 158 interactions.
DIPiDIP-1478N.
IntActiP43609. 93 interactions.
MINTiMINT-401637.
STRINGi4932.YFR037C.

Structurei

3D structure databases

ProteinModelPortaliP43609.
SMRiP43609. Positions 86-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 17798SWIRMPROSITE-ProRule annotationAdd
BLAST
Domaini310 – 36253SANTPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili38 – 7740Sequence AnalysisAdd
BLAST
Coiled coili462 – 49433Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 SANT domain.PROSITE-ProRule annotation
Contains 1 SWIRM domain.PROSITE-ProRule annotation
Contains 1 ZZ-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri254 – 29845ZZ-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiCOG5259.
HOGENOMiHOG000246692.
InParanoidiP43609.
KOiK11762.
OMAiKTPKAYK.
OrthoDBiEOG7ZWDBR.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43609-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE
60 70 80 90 100
VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWFD ISKIHEIEKR
110 120 130 140 150
SNPDFFNDSS RFKTPKAYKD TRNFIINTYR LSPYEYLTIT AVRRNVAMDV
160 170 180 190 200
ASIVKIHAFL EKWGLINYQI DPRTKPSLIG PSFTGHFQVV LDTPQGLKPF
210 220 230 240 250
LPENVIKQEV EGGDGAEPQV KKEFPVNLTI KKNVYDSAQD FNALQDESRN
260 270 280 290 300
SRQIHKVYIC HTCGNESINV RYHNLRARDT NLCSRCFQEG HFGANFQSSD
310 320 330 340 350
FIRLENNGNS VKKNWSDQEM LLLLEGIEMY EDQWEKIADH VGGHKRVEDC
360 370 380 390 400
IEKFLSLPIE DNYIREVVGS TLNGKGGDSR DGSVSGSKLM ECVNDAVQTL
410 420 430 440 450
LQGDDKLGKV SDKSREISEK YIEESQAIIQ ELVKLTMEKL ESKFTKLCDL
460 470 480 490 500
ETQLEMEKLK YVKESEKMLN DRLSLSKQIL DLNKSLEELN VSKKLVLISE
510 520 530 540 550
QVDSGIQLVE KDQEGDDEDG NTATGHGVKR VGKEGEEVGE GDSIAKLQPQ

VYKPWSL
Length:557
Mass (Da):63,168
Last modified:November 1, 1995 - v1
Checksum:iD3D3120397CF0058
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921D → G in AAT92860. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09276.1.
AY692841 Genomic DNA. Translation: AAT92860.1.
BK006940 Genomic DNA. Translation: DAA12480.1.
PIRiS56292.
RefSeqiNP_116695.3. NM_001180002.3.

Genome annotation databases

EnsemblFungiiYFR037C; YFR037C; YFR037C.
GeneIDi850598.
KEGGisce:YFR037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09276.1 .
AY692841 Genomic DNA. Translation: AAT92860.1 .
BK006940 Genomic DNA. Translation: DAA12480.1 .
PIRi S56292.
RefSeqi NP_116695.3. NM_001180002.3.

3D structure databases

ProteinModelPortali P43609.
SMRi P43609. Positions 86-170.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31195. 158 interactions.
DIPi DIP-1478N.
IntActi P43609. 93 interactions.
MINTi MINT-401637.
STRINGi 4932.YFR037C.

Proteomic databases

MaxQBi P43609.
PaxDbi P43609.
PeptideAtlasi P43609.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR037C ; YFR037C ; YFR037C .
GeneIDi 850598.
KEGGi sce:YFR037C.

Organism-specific databases

CYGDi YFR037c.
SGDi S000001933. RSC8.

Phylogenomic databases

eggNOGi COG5259.
HOGENOMi HOG000246692.
InParanoidi P43609.
KOi K11762.
OMAi KTPKAYK.
OrthoDBi EOG7ZWDBR.

Enzyme and pathway databases

BioCyci YEAST:G3O-30484-MONOMER.

Miscellaneous databases

NextBioi 966460.

Gene expression databases

Genevestigatori P43609.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProi IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
IPR000433. Znf_ZZ.
[Graphical view ]
Pfami PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view ]
SMARTi SM00717. SANT. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 2 hits.
PROSITEi PS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
    Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
    Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: PROTEIN SEQUENCE OF 233-249, FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
  6. "Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae."
    Treich I., Carlson M.
    Mol. Cell. Biol. 17:1768-1775(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH STH1.
  7. "Histone octamer transfer by a chromatin-remodeling complex."
    Lorch Y., Zhang M., Kornberg R.D.
    Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  8. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
    Moreira J.M.A., Holmberg S.
    EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
    Saha A., Wittmeyer J., Cairns B.R.
    Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
    Chai B., Hsu J.-M., Du J., Laurent B.C.
    Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  11. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
    Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
    Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
    Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
    Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE RSC COMPLEX.
  14. "Direct interaction between Rsc6 and Rsc8/Swh3, two proteins that are conserved in SWI/SNF-related complexes."
    Treich I., Ho L., Carlson M.
    Nucleic Acids Res. 26:3739-3745(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RSC6.
  15. "Essential role for the SANT domain in the functioning of multiple chromatin remodeling enzymes."
    Boyer L.A., Langer M.R., Crowley K.A., Tan S., Denu J.M., Peterson C.L.
    Mol. Cell 10:935-942(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 347-VAL--ILE-351.
  16. "Dissecting the pet18 mutation in Saccharomyces cerevisiae: HTL1 encodes a 7-kDa polypeptide that interacts with components of the RSC complex."
    Lu Y.-M., Lin Y.-R., Tsai A., Hsao Y.-S., Li C.-C., Cheng M.Y.
    Mol. Genet. Genomics 269:321-330(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HTL1.
  17. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
    Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
    Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NPL6.
  18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRSC8_YEAST
AccessioniPrimary (citable) accession number: P43609
Secondary accession number(s): D6VTS0, Q6B289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3