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P43609

- RSC8_YEAST

UniProt

P43609 - RSC8_YEAST

Protein

Chromatin structure-remodeling complex protein RSC8

Gene

RSC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.7 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri254 – 29845ZZ-typeAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA binding Source: SGD
    3. protein binding Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. ATP-dependent chromatin remodeling Source: UniProtKB
    3. double-strand break repair via nonhomologous end joining Source: SGD
    4. G1/S transition of mitotic cell cycle Source: SGD
    5. nucleosome disassembly Source: SGD
    6. regulation of transcription, DNA-templated Source: UniProtKB
    7. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30484-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromatin structure-remodeling complex protein RSC8
    Alternative name(s):
    Remodel the structure of chromatin complex subunit 8
    SWI3 homolog
    Gene namesi
    Name:RSC8
    Synonyms:SWH3
    Ordered Locus Names:YFR037C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR037c.
    SGDiS000001933. RSC8.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation
    Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

    GO - Cellular componenti

    1. RSC complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi347 – 3515Missing: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 557557Chromatin structure-remodeling complex protein RSC8PRO_0000197114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei485 – 4851Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43609.
    PaxDbiP43609.
    PeptideAtlasiP43609.

    Expressioni

    Gene expression databases

    GenevestigatoriP43609.

    Interactioni

    Subunit structurei

    Dimerizes via the C-terminal coiled coil. Interacts directly with HTL1, NPL6, RSC6 and the N-terminus of STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HTL1Q9URQ53EBI-23005,EBI-8717
    NPL6P328327EBI-23005,EBI-12202
    RSC6P2563212EBI-23005,EBI-21941
    RSC9Q031244EBI-23005,EBI-27977

    Protein-protein interaction databases

    BioGridi31195. 158 interactions.
    DIPiDIP-1478N.
    IntActiP43609. 93 interactions.
    MINTiMINT-401637.
    STRINGi4932.YFR037C.

    Structurei

    3D structure databases

    ProteinModelPortaliP43609.
    SMRiP43609. Positions 86-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 17798SWIRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini310 – 36253SANTPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili38 – 7740Sequence AnalysisAdd
    BLAST
    Coiled coili462 – 49433Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SANT domain.PROSITE-ProRule annotation
    Contains 1 SWIRM domain.PROSITE-ProRule annotation
    Contains 1 ZZ-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri254 – 29845ZZ-typeAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5259.
    HOGENOMiHOG000246692.
    KOiK11762.
    OMAiKTPKAYK.
    OrthoDBiEOG7ZWDBR.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.10.60. 1 hit.
    InterProiIPR009057. Homeodomain-like.
    IPR001005. SANT/Myb.
    IPR017884. SANT_dom.
    IPR007526. SWIRM.
    IPR011991. WHTH_DNA-bd_dom.
    IPR000433. Znf_ZZ.
    [Graphical view]
    PfamiPF00249. Myb_DNA-binding. 1 hit.
    PF04433. SWIRM. 1 hit.
    PF00569. ZZ. 1 hit.
    [Graphical view]
    SMARTiSM00717. SANT. 1 hit.
    SM00291. ZnF_ZZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 2 hits.
    PROSITEiPS51293. SANT. 1 hit.
    PS50934. SWIRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43609-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE    50
    VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWFD ISKIHEIEKR 100
    SNPDFFNDSS RFKTPKAYKD TRNFIINTYR LSPYEYLTIT AVRRNVAMDV 150
    ASIVKIHAFL EKWGLINYQI DPRTKPSLIG PSFTGHFQVV LDTPQGLKPF 200
    LPENVIKQEV EGGDGAEPQV KKEFPVNLTI KKNVYDSAQD FNALQDESRN 250
    SRQIHKVYIC HTCGNESINV RYHNLRARDT NLCSRCFQEG HFGANFQSSD 300
    FIRLENNGNS VKKNWSDQEM LLLLEGIEMY EDQWEKIADH VGGHKRVEDC 350
    IEKFLSLPIE DNYIREVVGS TLNGKGGDSR DGSVSGSKLM ECVNDAVQTL 400
    LQGDDKLGKV SDKSREISEK YIEESQAIIQ ELVKLTMEKL ESKFTKLCDL 450
    ETQLEMEKLK YVKESEKMLN DRLSLSKQIL DLNKSLEELN VSKKLVLISE 500
    QVDSGIQLVE KDQEGDDEDG NTATGHGVKR VGKEGEEVGE GDSIAKLQPQ 550
    VYKPWSL 557
    Length:557
    Mass (Da):63,168
    Last modified:November 1, 1995 - v1
    Checksum:iD3D3120397CF0058
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti192 – 1921D → G in AAT92860. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09276.1.
    AY692841 Genomic DNA. Translation: AAT92860.1.
    BK006940 Genomic DNA. Translation: DAA12480.1.
    PIRiS56292.
    RefSeqiNP_116695.3. NM_001180002.3.

    Genome annotation databases

    EnsemblFungiiYFR037C; YFR037C; YFR037C.
    GeneIDi850598.
    KEGGisce:YFR037C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09276.1 .
    AY692841 Genomic DNA. Translation: AAT92860.1 .
    BK006940 Genomic DNA. Translation: DAA12480.1 .
    PIRi S56292.
    RefSeqi NP_116695.3. NM_001180002.3.

    3D structure databases

    ProteinModelPortali P43609.
    SMRi P43609. Positions 86-170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31195. 158 interactions.
    DIPi DIP-1478N.
    IntActi P43609. 93 interactions.
    MINTi MINT-401637.
    STRINGi 4932.YFR037C.

    Proteomic databases

    MaxQBi P43609.
    PaxDbi P43609.
    PeptideAtlasi P43609.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR037C ; YFR037C ; YFR037C .
    GeneIDi 850598.
    KEGGi sce:YFR037C.

    Organism-specific databases

    CYGDi YFR037c.
    SGDi S000001933. RSC8.

    Phylogenomic databases

    eggNOGi COG5259.
    HOGENOMi HOG000246692.
    KOi K11762.
    OMAi KTPKAYK.
    OrthoDBi EOG7ZWDBR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30484-MONOMER.

    Miscellaneous databases

    NextBioi 966460.

    Gene expression databases

    Genevestigatori P43609.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.10.60. 1 hit.
    InterProi IPR009057. Homeodomain-like.
    IPR001005. SANT/Myb.
    IPR017884. SANT_dom.
    IPR007526. SWIRM.
    IPR011991. WHTH_DNA-bd_dom.
    IPR000433. Znf_ZZ.
    [Graphical view ]
    Pfami PF00249. Myb_DNA-binding. 1 hit.
    PF04433. SWIRM. 1 hit.
    PF00569. ZZ. 1 hit.
    [Graphical view ]
    SMARTi SM00717. SANT. 1 hit.
    SM00291. ZnF_ZZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 2 hits.
    PROSITEi PS51293. SANT. 1 hit.
    PS50934. SWIRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
      Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: PROTEIN SEQUENCE OF 233-249, FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
    6. "Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae."
      Treich I., Carlson M.
      Mol. Cell. Biol. 17:1768-1775(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH STH1.
    7. "Histone octamer transfer by a chromatin-remodeling complex."
      Lorch Y., Zhang M., Kornberg R.D.
      Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    8. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
      Moreira J.M.A., Holmberg S.
      EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
      Saha A., Wittmeyer J., Cairns B.R.
      Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
      Chai B., Hsu J.-M., Du J., Laurent B.C.
      Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    11. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
      Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
      Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
      Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
      Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF THE RSC COMPLEX.
    14. "Direct interaction between Rsc6 and Rsc8/Swh3, two proteins that are conserved in SWI/SNF-related complexes."
      Treich I., Ho L., Carlson M.
      Nucleic Acids Res. 26:3739-3745(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RSC6.
    15. "Essential role for the SANT domain in the functioning of multiple chromatin remodeling enzymes."
      Boyer L.A., Langer M.R., Crowley K.A., Tan S., Denu J.M., Peterson C.L.
      Mol. Cell 10:935-942(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 347-VAL--ILE-351.
    16. "Dissecting the pet18 mutation in Saccharomyces cerevisiae: HTL1 encodes a 7-kDa polypeptide that interacts with components of the RSC complex."
      Lu Y.-M., Lin Y.-R., Tsai A., Hsao Y.-S., Li C.-C., Cheng M.Y.
      Mol. Genet. Genomics 269:321-330(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HTL1.
    17. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
      Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
      Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NPL6.
    18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRSC8_YEAST
    AccessioniPrimary (citable) accession number: P43609
    Secondary accession number(s): D6VTS0, Q6B289
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3380 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3