Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43606

- PTR3_YEAST

UniProt

P43606 - PTR3_YEAST

Protein

SPS-sensor component PTR3

Gene

PTR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2.6 Publications

    GO - Biological processi

    1. response to amino acid Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30478-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SPS-sensor component PTR3
    Alternative name(s):
    Peptide transport protein 3
    Gene namesi
    Name:PTR3
    Synonyms:APF3, SSY3
    Ordered Locus Names:YFR029W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR029w.
    SGDiS000001925. PTR3.

    Subcellular locationi

    Cell membrane 2 Publications; Peripheral membrane protein 2 Publications; Cytoplasmic side 2 Publications

    GO - Cellular componenti

    1. extrinsic component of plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi321 – 3211S → A: No effect. 1 Publication
    Mutagenesisi435 – 4351T → K in PTR3-17; constitutively active, confers increased STP1 processing in the absence of amino acids. 2 Publications
    Mutagenesisi439 – 4391Q → R in PTR3-5; constitutively active and hyperphosphorylated, confers increased STP1 processing in the absence of amino acids. 2 Publications
    Mutagenesisi525 – 5251T → A, D or E: Loss of function. Abolishes hyperphosphorylation of PTR3 and, consequently, results in a failure to activate STP1. 1 Publication
    Mutagenesisi635 – 6351T → A: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 678678SPS-sensor component PTR3PRO_0000097093Add
    BLAST

    Post-translational modificationi

    Hyperphosphorylated in response to extracellular amino acids and dependent on the amino acid sensor component SSY1. Phosphorylation is positively regulated by casein kinases YCK1 and YCK2, and negatively regulated by phosphatase PP2A regulatory subunit RTS1.1 Publication

    Proteomic databases

    MaxQBiP43606.
    PaxDbiP43606.

    Expressioni

    Gene expression databases

    GenevestigatoriP43606.

    Interactioni

    Subunit structurei

    Homodimer. Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with SSY1 and SSY5.2 Publications

    Protein-protein interaction databases

    BioGridi31184. 74 interactions.
    IntActiP43606. 1 interaction.
    MINTiMINT-7915310.
    STRINGi4932.YFR029W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43606.
    SMRiP43606. Positions 386-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG47443.
    HOGENOMiHOG000111158.
    OMAiTIPYRDP.
    OrthoDBiEOG7GBG5D.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43606-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHSHRQKWGR QTDIARVLDD IEHDLYLPQR LSLDGATGTD ESHVQYGIVK    50
    DCSVLTCGCC ISESLFNDLC RETSNKQTAC PICQRENVRL LSAIKPLRDL 100
    ARQIDFFRST TGQGESESDE LPAIVKTSPS SSSLSLTPSR SSSTAGLEAD 150
    NKTLSDPTVK EKSSLLELFH IVASKMHNAN TEVGSDHPLT TGTTRDQEEH 200
    TTKENYSSSL LEPNYDDHAN WKILDNASNT RTVPIDNNFS LMSTDVTIPS 250
    TANYQTNSAH DLDEEKEYFF ANCFPMYRKK FQFNTHPKFL GTKSKLFINQ 300
    SISPDCTKFA LITEHKWEIY SINPKDNSPQ LVSCGKSSGE YGPNFNQLTE 350
    QSSSSLSTTS QASKKKKKNW SQRFCKLSND FLIISGSQNI LNVHDIHQNG 400
    KLIYTYVSRF PIRCIDIDPR SQIIAYGITG KDRHTGAEQA LVVIQQITRN 450
    KVTLEPEFPP PITITLPYRD PINTIQLSHD AKYLTCSTAL ESRFLIISLQ 500
    KINEPRLIMK SVRSIDTSLE SEGITDTKLF PGNPNLMCIT STAFNSSPLV 550
    INTKITQING VRTVAQPSML IRVDEIGCKI HKCEISPRND AIAFLDRNGS 600
    VYIMCAPTMM DNNEKRRTIL VETVANAYRA YESATLRFNP EGNKLYILDR 650
    KGTFFVEDFA YGLPQSREIT KCKQIFHK 678
    Length:678
    Mass (Da):76,286
    Last modified:November 1, 1995 - v1
    Checksum:iBF2B64842D669D6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09268.1.
    BK006940 Genomic DNA. Translation: DAA12469.1.
    PIRiS56284.
    RefSeqiNP_116685.1. NM_001179994.1.

    Genome annotation databases

    EnsemblFungiiYFR029W; YFR029W; YFR029W.
    GeneIDi850587.
    KEGGisce:YFR029W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09268.1 .
    BK006940 Genomic DNA. Translation: DAA12469.1 .
    PIRi S56284.
    RefSeqi NP_116685.1. NM_001179994.1.

    3D structure databases

    ProteinModelPortali P43606.
    SMRi P43606. Positions 386-427.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31184. 74 interactions.
    IntActi P43606. 1 interaction.
    MINTi MINT-7915310.
    STRINGi 4932.YFR029W.

    Proteomic databases

    MaxQBi P43606.
    PaxDbi P43606.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR029W ; YFR029W ; YFR029W .
    GeneIDi 850587.
    KEGGi sce:YFR029W.

    Organism-specific databases

    CYGDi YFR029w.
    SGDi S000001925. PTR3.

    Phylogenomic databases

    eggNOGi NOG47443.
    HOGENOMi HOG000111158.
    OMAi TIPYRDP.
    OrthoDBi EOG7GBG5D.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30478-MONOMER.

    Miscellaneous databases

    NextBioi 966428.

    Gene expression databases

    Genevestigatori P43606.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
      Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. "PTR3, a novel gene mediating amino acid-inducible regulation of peptide transport in Saccharomyces cerevisiae."
      Barnes D., Lai W., Breslav M., Naider F., Becker J.M.
      Mol. Microbiol. 29:297-310(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast."
      Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C.
      Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    6. "Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids."
      Klasson H., Fink G.R., Ljungdahl P.O.
      Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids."
      Forsberg H., Ljungdahl P.O.
      Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae."
      Bernard F., Andre B.
      Mol. Microbiol. 41:489-502(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SSY5.
    9. "Receptor-mediated endoproteolytic activation of two transcription factors in yeast."
      Andreasson C., Ljungdahl P.O.
      Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae."
      Poulsen P., Wu B., Gaber R.F., Kielland-Brandt M.C.
      Eukaryot. Cell 4:1116-1124(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-435 AND GLN-439.
    11. "Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3."
      Liu Z., Thornton J., Spirek M., Butow R.A.
      Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH SSY1 AND SSY5, MUTAGENESIS OF SER-321; THR-435; GLN-439; THR-525 AND THR-635.

    Entry informationi

    Entry nameiPTR3_YEAST
    AccessioniPrimary (citable) accession number: P43606
    Secondary accession number(s): D6VTQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3