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P43606 (PTR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SPS-sensor component PTR3
Alternative name(s):
Peptide transport protein 3
Gene names
Name:PTR3
Synonyms:APF3, SSY3
Ordered Locus Names:YFR029W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11

Subunit structure

Homodimer. Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with SSY1 and SSY5. Ref.8 Ref.11

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.6 Ref.7.

Post-translational modification

Hyperphosphorylated in response to extracellular amino acids and dependent on the amino acid sensor component SSY1. Phosphorylation is positively regulated by casein kinases YCK1 and YCK2, and negatively regulated by phosphatase PP2A regulatory subunit RTS1. Ref.11

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to amino acid

Inferred from mutant phenotype Ref.6. Source: SGD

   Cellular_componentextrinsic component of plasma membrane

Inferred from direct assay Ref.6. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 678678SPS-sensor component PTR3
PRO_0000097093

Experimental info

Mutagenesis3211S → A: No effect. Ref.11
Mutagenesis4351T → K in PTR3-17; constitutively active, confers increased STP1 processing in the absence of amino acids. Ref.10 Ref.11
Mutagenesis4391Q → R in PTR3-5; constitutively active and hyperphosphorylated, confers increased STP1 processing in the absence of amino acids. Ref.10 Ref.11
Mutagenesis5251T → A, D or E: Loss of function. Abolishes hyperphosphorylation of PTR3 and, consequently, results in a failure to activate STP1. Ref.11
Mutagenesis6351T → A: No effect. Ref.11

Sequences

Sequence LengthMass (Da)Tools
P43606 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: BF2B64842D669D6B

FASTA67876,286
        10         20         30         40         50         60 
MHSHRQKWGR QTDIARVLDD IEHDLYLPQR LSLDGATGTD ESHVQYGIVK DCSVLTCGCC 

        70         80         90        100        110        120 
ISESLFNDLC RETSNKQTAC PICQRENVRL LSAIKPLRDL ARQIDFFRST TGQGESESDE 

       130        140        150        160        170        180 
LPAIVKTSPS SSSLSLTPSR SSSTAGLEAD NKTLSDPTVK EKSSLLELFH IVASKMHNAN 

       190        200        210        220        230        240 
TEVGSDHPLT TGTTRDQEEH TTKENYSSSL LEPNYDDHAN WKILDNASNT RTVPIDNNFS 

       250        260        270        280        290        300 
LMSTDVTIPS TANYQTNSAH DLDEEKEYFF ANCFPMYRKK FQFNTHPKFL GTKSKLFINQ 

       310        320        330        340        350        360 
SISPDCTKFA LITEHKWEIY SINPKDNSPQ LVSCGKSSGE YGPNFNQLTE QSSSSLSTTS 

       370        380        390        400        410        420 
QASKKKKKNW SQRFCKLSND FLIISGSQNI LNVHDIHQNG KLIYTYVSRF PIRCIDIDPR 

       430        440        450        460        470        480 
SQIIAYGITG KDRHTGAEQA LVVIQQITRN KVTLEPEFPP PITITLPYRD PINTIQLSHD 

       490        500        510        520        530        540 
AKYLTCSTAL ESRFLIISLQ KINEPRLIMK SVRSIDTSLE SEGITDTKLF PGNPNLMCIT 

       550        560        570        580        590        600 
STAFNSSPLV INTKITQING VRTVAQPSML IRVDEIGCKI HKCEISPRND AIAFLDRNGS 

       610        620        630        640        650        660 
VYIMCAPTMM DNNEKRRTIL VETVANAYRA YESATLRFNP EGNKLYILDR KGTFFVEDFA 

       670 
YGLPQSREIT KCKQIFHK 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]"PTR3, a novel gene mediating amino acid-inducible regulation of peptide transport in Saccharomyces cerevisiae."
Barnes D., Lai W., Breslav M., Naider F., Becker J.M.
Mol. Microbiol. 29:297-310(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast."
Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C.
Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids."
Klasson H., Fink G.R., Ljungdahl P.O.
Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids."
Forsberg H., Ljungdahl P.O.
Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae."
Bernard F., Andre B.
Mol. Microbiol. 41:489-502(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SSY5.
[9]"Receptor-mediated endoproteolytic activation of two transcription factors in yeast."
Andreasson C., Ljungdahl P.O.
Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae."
Poulsen P., Wu B., Gaber R.F., Kielland-Brandt M.C.
Eukaryot. Cell 4:1116-1124(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF THR-435 AND GLN-439.
[11]"Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3."
Liu Z., Thornton J., Spirek M., Butow R.A.
Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH SSY1 AND SSY5, MUTAGENESIS OF SER-321; THR-435; GLN-439; THR-525 AND THR-635.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50617 Genomic DNA. Translation: BAA09268.1.
BK006940 Genomic DNA. Translation: DAA12469.1.
PIRS56284.
RefSeqNP_116685.1. NM_001179994.1.

3D structure databases

ProteinModelPortalP43606.
SMRP43606. Positions 379-427, 577-604, 635-662.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31184. 74 interactions.
IntActP43606. 1 interaction.
MINTMINT-7915310.
STRING4932.YFR029W.

Proteomic databases

PaxDbP43606.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR029W; YFR029W; YFR029W.
GeneID850587.
KEGGsce:YFR029W.

Organism-specific databases

CYGDYFR029w.
SGDS000001925. PTR3.

Phylogenomic databases

eggNOGNOG47443.
HOGENOMHOG000111158.
OMATIPYRDP.
OrthoDBEOG7GBG5D.

Enzyme and pathway databases

BioCycYEAST:G3O-30478-MONOMER.

Gene expression databases

GenevestigatorP43606.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
ProtoNetSearch...

Other

NextBio966428.

Entry information

Entry namePTR3_YEAST
AccessionPrimary (citable) accession number: P43606
Secondary accession number(s): D6VTQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD