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Protein

SPS-sensor component PTR3

Gene

PTR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2.6 Publications

GO - Biological processi

  • response to amino acid Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30478-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SPS-sensor component PTR3
Alternative name(s):
Peptide transport protein 3
Gene namesi
Name:PTR3
Synonyms:APF3, SSY3
Ordered Locus Names:YFR029W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VI

Organism-specific databases

CYGDiYFR029w.
EuPathDBiFungiDB:YFR029W.
SGDiS000001925. PTR3.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi321 – 3211S → A: No effect. 1 Publication
Mutagenesisi435 – 4351T → K in PTR3-17; constitutively active, confers increased STP1 processing in the absence of amino acids. 2 Publications
Mutagenesisi439 – 4391Q → R in PTR3-5; constitutively active and hyperphosphorylated, confers increased STP1 processing in the absence of amino acids. 2 Publications
Mutagenesisi525 – 5251T → A, D or E: Loss of function. Abolishes hyperphosphorylation of PTR3 and, consequently, results in a failure to activate STP1. 1 Publication
Mutagenesisi635 – 6351T → A: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 678678SPS-sensor component PTR3PRO_0000097093Add
BLAST

Post-translational modificationi

Hyperphosphorylated in response to extracellular amino acids and dependent on the amino acid sensor component SSY1. Phosphorylation is positively regulated by casein kinases YCK1 and YCK2, and negatively regulated by phosphatase PP2A regulatory subunit RTS1.1 Publication

Proteomic databases

MaxQBiP43606.
PaxDbiP43606.

Interactioni

Subunit structurei

Homodimer. Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with SSY1 and SSY5.2 Publications

Protein-protein interaction databases

BioGridi31184. 74 interactions.
IntActiP43606. 1 interaction.
MINTiMINT-7915310.

Structurei

3D structure databases

ProteinModelPortaliP43606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG47443.
HOGENOMiHOG000111158.
InParanoidiP43606.
OMAiTIPYRDP.
OrthoDBiEOG7GBG5D.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43606-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSHRQKWGR QTDIARVLDD IEHDLYLPQR LSLDGATGTD ESHVQYGIVK
60 70 80 90 100
DCSVLTCGCC ISESLFNDLC RETSNKQTAC PICQRENVRL LSAIKPLRDL
110 120 130 140 150
ARQIDFFRST TGQGESESDE LPAIVKTSPS SSSLSLTPSR SSSTAGLEAD
160 170 180 190 200
NKTLSDPTVK EKSSLLELFH IVASKMHNAN TEVGSDHPLT TGTTRDQEEH
210 220 230 240 250
TTKENYSSSL LEPNYDDHAN WKILDNASNT RTVPIDNNFS LMSTDVTIPS
260 270 280 290 300
TANYQTNSAH DLDEEKEYFF ANCFPMYRKK FQFNTHPKFL GTKSKLFINQ
310 320 330 340 350
SISPDCTKFA LITEHKWEIY SINPKDNSPQ LVSCGKSSGE YGPNFNQLTE
360 370 380 390 400
QSSSSLSTTS QASKKKKKNW SQRFCKLSND FLIISGSQNI LNVHDIHQNG
410 420 430 440 450
KLIYTYVSRF PIRCIDIDPR SQIIAYGITG KDRHTGAEQA LVVIQQITRN
460 470 480 490 500
KVTLEPEFPP PITITLPYRD PINTIQLSHD AKYLTCSTAL ESRFLIISLQ
510 520 530 540 550
KINEPRLIMK SVRSIDTSLE SEGITDTKLF PGNPNLMCIT STAFNSSPLV
560 570 580 590 600
INTKITQING VRTVAQPSML IRVDEIGCKI HKCEISPRND AIAFLDRNGS
610 620 630 640 650
VYIMCAPTMM DNNEKRRTIL VETVANAYRA YESATLRFNP EGNKLYILDR
660 670
KGTFFVEDFA YGLPQSREIT KCKQIFHK
Length:678
Mass (Da):76,286
Last modified:November 1, 1995 - v1
Checksum:iBF2B64842D669D6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09268.1.
BK006940 Genomic DNA. Translation: DAA12469.1.
PIRiS56284.
RefSeqiNP_116685.1. NM_001179994.1.

Genome annotation databases

EnsemblFungiiYFR029W; YFR029W; YFR029W.
GeneIDi850587.
KEGGisce:YFR029W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09268.1.
BK006940 Genomic DNA. Translation: DAA12469.1.
PIRiS56284.
RefSeqiNP_116685.1. NM_001179994.1.

3D structure databases

ProteinModelPortaliP43606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31184. 74 interactions.
IntActiP43606. 1 interaction.
MINTiMINT-7915310.

Proteomic databases

MaxQBiP43606.
PaxDbiP43606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFR029W; YFR029W; YFR029W.
GeneIDi850587.
KEGGisce:YFR029W.

Organism-specific databases

CYGDiYFR029w.
EuPathDBiFungiDB:YFR029W.
SGDiS000001925. PTR3.

Phylogenomic databases

eggNOGiNOG47443.
HOGENOMiHOG000111158.
InParanoidiP43606.
OMAiTIPYRDP.
OrthoDBiEOG7GBG5D.

Enzyme and pathway databases

BioCyciYEAST:G3O-30478-MONOMER.

Miscellaneous databases

NextBioi966428.
PROiP43606.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
    Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
    Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. "PTR3, a novel gene mediating amino acid-inducible regulation of peptide transport in Saccharomyces cerevisiae."
    Barnes D., Lai W., Breslav M., Naider F., Becker J.M.
    Mol. Microbiol. 29:297-310(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast."
    Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C.
    Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids."
    Klasson H., Fink G.R., Ljungdahl P.O.
    Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids."
    Forsberg H., Ljungdahl P.O.
    Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae."
    Bernard F., Andre B.
    Mol. Microbiol. 41:489-502(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SSY5.
  9. "Receptor-mediated endoproteolytic activation of two transcription factors in yeast."
    Andreasson C., Ljungdahl P.O.
    Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae."
    Poulsen P., Wu B., Gaber R.F., Kielland-Brandt M.C.
    Eukaryot. Cell 4:1116-1124(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-435 AND GLN-439.
  11. "Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3."
    Liu Z., Thornton J., Spirek M., Butow R.A.
    Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH SSY1 AND SSY5, MUTAGENESIS OF SER-321; THR-435; GLN-439; THR-525 AND THR-635.

Entry informationi

Entry nameiPTR3_YEAST
AccessioniPrimary (citable) accession number: P43606
Secondary accession number(s): D6VTQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.