P43606 (PTR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SPS-sensor component PTR3

Alternative name(s):
Peptide transport protein 3

Gene names

Name:	PTR3
Synonyms:	APF3, SSY3
Ordered Locus Names:	YFR029W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11
Subunit structure	Homodimer. Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. Interacts directly with SSY1 and SSY5. Ref.8 Ref.11
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.6 Ref.7.
Post-translational modification	Hyperphosphorylated in response to extracellular amino acids and dependent on the amino acid sensor component SSY1. Phosphorylation is positively regulated by casein kinases YCK1 and YCK2, and negatively regulated by phosphatase PP2A regulatory subunit RTS1. Ref.11

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to amino acid stimulus Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	extrinsic to plasma membrane Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	SPS-sensor component PTR3		PRO_0000097093
Experimental info
	Mutagenesis	321	1	S → A: No effect. Ref.11
	Mutagenesis	435	1	T → K in PTR3-17; constitutively active, confers increased STP1 processing in the absence of amino-acids. Ref.10 Ref.11
	Mutagenesis	439	1	Q → R in PTR3-5; constitutively active and hyperphosphorylated, confers increased STP1 processing in the absence of amino-acids. Ref.10 Ref.11
	Mutagenesis	525	1	T → A, D or E: Loss of function. Abolishes hyperphosphorylation of PTR3 and, consequently, results in a failure to activate STP1. Ref.11
	Mutagenesis	635	1	T → A: No effect. Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P43606 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BF2B64842D669D6B	FASTA	678	76,286
	10 20 30 40 50 60 MHSHRQKWGR QTDIARVLDD IEHDLYLPQR LSLDGATGTD ESHVQYGIVK DCSVLTCGCC 70 80 90 100 110 120 ISESLFNDLC RETSNKQTAC PICQRENVRL LSAIKPLRDL ARQIDFFRST TGQGESESDE 130 140 150 160 170 180 LPAIVKTSPS SSSLSLTPSR SSSTAGLEAD NKTLSDPTVK EKSSLLELFH IVASKMHNAN 190 200 210 220 230 240 TEVGSDHPLT TGTTRDQEEH TTKENYSSSL LEPNYDDHAN WKILDNASNT RTVPIDNNFS 250 260 270 280 290 300 LMSTDVTIPS TANYQTNSAH DLDEEKEYFF ANCFPMYRKK FQFNTHPKFL GTKSKLFINQ 310 320 330 340 350 360 SISPDCTKFA LITEHKWEIY SINPKDNSPQ LVSCGKSSGE YGPNFNQLTE QSSSSLSTTS 370 380 390 400 410 420 QASKKKKKNW SQRFCKLSND FLIISGSQNI LNVHDIHQNG KLIYTYVSRF PIRCIDIDPR 430 440 450 460 470 480 SQIIAYGITG KDRHTGAEQA LVVIQQITRN KVTLEPEFPP PITITLPYRD PINTIQLSHD 490 500 510 520 530 540 AKYLTCSTAL ESRFLIISLQ KINEPRLIMK SVRSIDTSLE SEGITDTKLF PGNPNLMCIT 550 560 570 580 590 600 STAFNSSPLV INTKITQING VRTVAQPSML IRVDEIGCKI HKCEISPRND AIAFLDRNGS 610 620 630 640 650 660 VYIMCAPTMM DNNEKRRTIL VETVANAYRA YESATLRFNP EGNKLYILDR KGTFFVEDFA 670 YGLPQSREIT KCKQIFHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"PTR3, a novel gene mediating amino acid-inducible regulation of peptide transport in Saccharomyces cerevisiae." Barnes D., Lai W., Breslav M., Naider F., Becker J.M. Mol. Microbiol. 29:297-310(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast." Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C. Yeast 14:103-114(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids." Klasson H., Fink G.R., Ljungdahl P.O. Mol. Cell. Biol. 19:5405-5416(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids." Forsberg H., Ljungdahl P.O. Mol. Cell. Biol. 21:814-826(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae." Bernard F., Andre B. Mol. Microbiol. 41:489-502(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SSY5.
[9]	"Receptor-mediated endoproteolytic activation of two transcription factors in yeast." Andreasson C., Ljungdahl P.O. Genes Dev. 16:3158-3172(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae." Poulsen P., Wu B., Gaber R.F., Kielland-Brandt M.C. Eukaryot. Cell 4:1116-1124(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF THR-435 AND GLN-439.
[11]	"Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3." Liu Z., Thornton J., Spirek M., Butow R.A. Mol. Cell. Biol. 28:551-563(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH SSY1 AND SSY5, MUTAGENESIS OF SER-321; THR-435; GLN-439; THR-525 AND THR-635.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09268.1. BK006940 Genomic DNA. Translation: DAA12469.1.
PIR	S56284.
RefSeq	NP_116685.1. NM_001179994.1.
3D structure databases
ProteinModelPortal	P43606.
SMR	P43606. Positions 468-499.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43606. 1 interaction.
STRING	4932.YFR029W.
Proteomic databases
PaxDb	P43606.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR029W; YFR029W; YFR029W.
GeneID	850587.
KEGG	sce:YFR029W.
Organism-specific databases
CYGD	YFR029w.
SGD	S000001925. PTR3.
Phylogenomic databases
eggNOG	NOG47443.
HOGENOM	HOG000111158.
OMA	TIPYRDP.
OrthoDB	EOG4WHCVD.
Gene expression databases
Genevestigator	P43606.
GermOnline	YFR029W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR017986. WD40_repeat_dom. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
ProtoNet	Search...
Other
NextBio	966428.

Entry information

Entry name

PTR3_YEAST

Accession

Primary (citable) accession number: P43606
Secondary accession number(s): D6VTQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program