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P43605

- ECO1_YEAST

UniProt

P43605 - ECO1_YEAST

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Protein

N-acetyltransferase ECO1

Gene

ECO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA. Interacts with the three known alternate replication factor C (RFC) complexes, suggesting that these complexes have essential but redundant activity in cohesion establishment. Acts by acetylating the cohesin complex component SMC3. In vitro, possesses acetyltransferase activity where it can acetylate itself and components of the cohesin complex (MCD1, IRR1 and PDS5), but is unable to acetylate histones.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri33 – 5725CCHH-typeAdd
BLAST

GO - Molecular functioni

  1. acetyltransferase activity Source: UniProtKB
  2. chromatin binding Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. chromosome organization Source: SGD
  2. DNA repair Source: SGD
  3. DNA replication Source: SGD
  4. double-strand break repair Source: SGD
  5. establishment of mitotic sister chromatid cohesion Source: SGD
  6. internal peptidyl-lysine acetylation Source: SGD
  7. mitotic chromosome condensation Source: SGD
  8. regulation of mitosis Source: UniProtKB
  9. telomere organization Source: SGD
  10. tRNA gene clustering Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30476-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyltransferase ECO1 (EC:2.3.1.-)
Alternative name(s):
Chromosome transmission fidelity protein 7
Establishment of cohesion protein 1
Gene namesi
Name:ECO1
Synonyms:CTF7
Ordered Locus Names:YFR027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR027w.
SGDiS000001923. ECO1.

Subcellular locationi

Nucleus 2 Publications
Note: Associated with chromatin.

GO - Cellular componenti

  1. nuclear chromatin Source: UniProtKB
  2. nuclear replication fork Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351C → Y in ctf7-109; loss of function. 1 Publication
Mutagenesisi53 – 531H → Y in ctf7-108; loss of function. 1 Publication
Mutagenesisi211 – 2111G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication
Mutagenesisi222 – 2232RK → GG: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication
Mutagenesisi225 – 2251G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication
Mutagenesisi232 – 2321D → G: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281N-acetyltransferase ECO1PRO_0000074550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei223 – 2231N6-acetyllysine; by autocatalysis1 Publication

Post-translational modificationi

Autoacetylates in vitro.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43605.
PeptideAtlasiP43605.

Expressioni

Gene expression databases

GenevestigatoriP43605.

Interactioni

Subunit structurei

Binds specifically to CHL12, RFC1, RFC2, RFC3, RFC4, RFC5 and RAD24 when members of an RFC complex. Interacts with CHL1 and MPS3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CHL1P225162EBI-22988,EBI-4600
CTF18P499562EBI-22988,EBI-4560
MPS3P470695EBI-22988,EBI-25811

Protein-protein interaction databases

BioGridi31181. 42 interactions.
DIPiDIP-5602N.
IntActiP43605. 13 interactions.
MINTiMINT-494620.
STRINGi4932.YFR027W.

Structurei

3D structure databases

ProteinModelPortaliP43605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetyltransferase family. ECO subfamily.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri33 – 5725CCHH-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG301368.
HOGENOMiHOG000093358.
InParanoidiP43605.
KOiK11268.
OMAiISRIWVC.
OrthoDBiEOG7D2FPZ.

Family and domain databases

InterProiIPR028005. AcTrfase_ESCO_Znf_dom.
IPR016181. Acyl_CoA_acyltransferase.
IPR028009. ESCO_Acetyltransf_dom.
[Graphical view]
PfamiPF13880. Acetyltransf_13. 1 hit.
PF13878. zf-C2H2_3. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.

Sequencei

Sequence statusi: Complete.

P43605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR
60 70 80 90 100
AIHEKYHTLQ LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP
110 120 130 140 150
LKKSSPSITH QEEKIVYVRP DKSNGEVRAM TEIMTLVNNE LNAPHDENVI
160 170 180 190 200
WNSTTEEKGK AFVYIRNDRA VGIIIIENLY GGNGKTSSRG RWMVYDSRRL
210 220 230 240 250
VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY GEVIPRYQVA
260 270 280
WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I
Length:281
Mass (Da):31,845
Last modified:November 1, 1995 - v1
Checksum:i0AF1B59BE9E71818
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09266.1.
BK006940 Genomic DNA. Translation: DAA12467.1.
PIRiS56282.
RefSeqiNP_116683.1. NM_001179992.1.

Genome annotation databases

EnsemblFungiiYFR027W; YFR027W; YFR027W.
GeneIDi850584.
KEGGisce:YFR027W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09266.1 .
BK006940 Genomic DNA. Translation: DAA12467.1 .
PIRi S56282.
RefSeqi NP_116683.1. NM_001179992.1.

3D structure databases

ProteinModelPortali P43605.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31181. 42 interactions.
DIPi DIP-5602N.
IntActi P43605. 13 interactions.
MINTi MINT-494620.
STRINGi 4932.YFR027W.

Proteomic databases

MaxQBi P43605.
PeptideAtlasi P43605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR027W ; YFR027W ; YFR027W .
GeneIDi 850584.
KEGGi sce:YFR027W.

Organism-specific databases

CYGDi YFR027w.
SGDi S000001923. ECO1.

Phylogenomic databases

eggNOGi NOG301368.
HOGENOMi HOG000093358.
InParanoidi P43605.
KOi K11268.
OMAi ISRIWVC.
OrthoDBi EOG7D2FPZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30476-MONOMER.

Miscellaneous databases

NextBioi 966420.

Gene expression databases

Genevestigatori P43605.

Family and domain databases

InterProi IPR028005. AcTrfase_ESCO_Znf_dom.
IPR016181. Acyl_CoA_acyltransferase.
IPR028009. ESCO_Acetyltransf_dom.
[Graphical view ]
Pfami PF13880. Acetyltransf_13. 1 hit.
PF13878. zf-C2H2_3. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery."
    Skibbens R.V., Corson L.B., Koshland D., Hieter P.
    Genes Dev. 13:307-319(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
    Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
    Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: ATCC 200060 / W303.
  3. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
    Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
    Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion."
    Ivanov D., Schleiffer A., Eisenhaber F., Mechtler K., Haering C.H., Nasmyth K.
    Curr. Biol. 12:323-328(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT LYS-223, ENZYME ACTIVITY, MUTAGENESIS OF GLY-211; 222-ARG-LYS-223; GLY-225 AND ASP-232.
  7. "Mechanical link between cohesion establishment and DNA replication: Ctf7p/Eco1p, a cohesion establishment factor, associates with three different replication factor C complexes."
    Kenna M.A., Skibbens R.V.
    Mol. Cell. Biol. 23:2999-3007(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CHL12; RFC1; RFC2; RFC3; RFC4; RFC5 AND RAD24.
  8. "Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion."
    Skibbens R.V.
    Genetics 166:33-42(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHL1.
  9. "The spindle pole body assembly component Mps3p/Nep98p functions in sister chromatid cohesion."
    Antoniacci L.M., Kenna M.A., Uetz P., Fields S., Skibbens R.V.
    J. Biol. Chem. 279:49542-49550(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPS3.
  10. "Ctf7p/Eco1p exhibits acetyltransferase activity -- but does it matter?"
    Brands A., Skibbens R.V.
    Curr. Biol. 15:R50-R51(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-35 AND HIS-53.
  11. "Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast."
    Zhang J., Shi X., Li Y., Kim B.J., Jia J., Huang Z., Yang T., Fu X., Jung S.Y., Wang Y., Zhang P., Kim S.T., Pan X., Qin J.
    Mol. Cell 31:143-151(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, AUTOACETYLATION, INTERACTION WITH SMC3.

Entry informationi

Entry nameiECO1_YEAST
AccessioniPrimary (citable) accession number: P43605
Secondary accession number(s): D6VTQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3