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P43605

- ECO1_YEAST

UniProt

P43605 - ECO1_YEAST

Protein

N-acetyltransferase ECO1

Gene

ECO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA. Interacts with the three known alternate replication factor C (RFC) complexes, suggesting that these complexes have essential but redundant activity in cohesion establishment. Acts by acetylating the cohesin complex component SMC3. In vitro, possesses acetyltransferase activity where it can acetylate itself and components of the cohesin complex (MCD1, IRR1 and PDS5), but is unable to acetylate histones.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri33 – 5725CCHH-typeAdd
    BLAST

    GO - Molecular functioni

    1. acetyltransferase activity Source: UniProtKB
    2. chromatin binding Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. chromosome organization Source: SGD
    2. DNA repair Source: SGD
    3. DNA replication Source: SGD
    4. double-strand break repair Source: SGD
    5. establishment of mitotic sister chromatid cohesion Source: SGD
    6. internal peptidyl-lysine acetylation Source: SGD
    7. mitotic chromosome condensation Source: SGD
    8. mitotic S phase Source: UniProtKB
    9. positive regulation of sister chromatid cohesion Source: UniProtKB
    10. regulation of DNA replication Source: UniProtKB
    11. regulation of mitosis Source: UniProtKB
    12. telomere organization Source: SGD
    13. tRNA gene clustering Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30476-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetyltransferase ECO1 (EC:2.3.1.-)
    Alternative name(s):
    Chromosome transmission fidelity protein 7
    Establishment of cohesion protein 1
    Gene namesi
    Name:ECO1
    Synonyms:CTF7
    Ordered Locus Names:YFR027W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR027w.
    SGDiS000001923. ECO1.

    Subcellular locationi

    Nucleus 2 Publications
    Note: Associated with chromatin.

    GO - Cellular componenti

    1. nuclear chromatin Source: UniProtKB
    2. nuclear replication fork Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi35 – 351C → Y in ctf7-109; loss of function. 1 Publication
    Mutagenesisi53 – 531H → Y in ctf7-108; loss of function. 1 Publication
    Mutagenesisi211 – 2111G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication
    Mutagenesisi222 – 2232RK → GG: Abolishes acetyltransferase activity; but not chromatid cohesion activity.
    Mutagenesisi225 – 2251G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication
    Mutagenesisi232 – 2321D → G: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281N-acetyltransferase ECO1PRO_0000074550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei223 – 2231N6-acetyllysine; by autocatalysis1 Publication

    Post-translational modificationi

    Autoacetylates in vitro.

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP43605.
    PeptideAtlasiP43605.

    Expressioni

    Gene expression databases

    GenevestigatoriP43605.

    Interactioni

    Subunit structurei

    Binds specifically to CHL12, RFC1, RFC2, RFC3, RFC4, RFC5 and RAD24 when members of an RFC complex. Interacts with CHL1 and MPS3.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CHL1P225162EBI-22988,EBI-4600
    CTF18P499562EBI-22988,EBI-4560
    MPS3P470695EBI-22988,EBI-25811

    Protein-protein interaction databases

    BioGridi31181. 42 interactions.
    DIPiDIP-5602N.
    IntActiP43605. 13 interactions.
    MINTiMINT-494620.
    STRINGi4932.YFR027W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43605.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acetyltransferase family. ECO subfamily.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri33 – 5725CCHH-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG301368.
    HOGENOMiHOG000093358.
    KOiK11268.
    OMAiISRIWVC.
    OrthoDBiEOG7D2FPZ.

    Family and domain databases

    InterProiIPR028005. AcTrfase_ESCO_Znf_dom.
    IPR016181. Acyl_CoA_acyltransferase.
    IPR028009. ESCO_Acetyltransf_dom.
    [Graphical view]
    PfamiPF13880. Acetyltransf_13. 1 hit.
    PF13878. zf-C2H2_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43605-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR    50
    AIHEKYHTLQ LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP 100
    LKKSSPSITH QEEKIVYVRP DKSNGEVRAM TEIMTLVNNE LNAPHDENVI 150
    WNSTTEEKGK AFVYIRNDRA VGIIIIENLY GGNGKTSSRG RWMVYDSRRL 200
    VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY GEVIPRYQVA 250
    WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I 281
    Length:281
    Mass (Da):31,845
    Last modified:November 1, 1995 - v1
    Checksum:i0AF1B59BE9E71818
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09266.1.
    BK006940 Genomic DNA. Translation: DAA12467.1.
    PIRiS56282.
    RefSeqiNP_116683.1. NM_001179992.1.

    Genome annotation databases

    EnsemblFungiiYFR027W; YFR027W; YFR027W.
    GeneIDi850584.
    KEGGisce:YFR027W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09266.1 .
    BK006940 Genomic DNA. Translation: DAA12467.1 .
    PIRi S56282.
    RefSeqi NP_116683.1. NM_001179992.1.

    3D structure databases

    ProteinModelPortali P43605.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31181. 42 interactions.
    DIPi DIP-5602N.
    IntActi P43605. 13 interactions.
    MINTi MINT-494620.
    STRINGi 4932.YFR027W.

    Proteomic databases

    MaxQBi P43605.
    PeptideAtlasi P43605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR027W ; YFR027W ; YFR027W .
    GeneIDi 850584.
    KEGGi sce:YFR027W.

    Organism-specific databases

    CYGDi YFR027w.
    SGDi S000001923. ECO1.

    Phylogenomic databases

    eggNOGi NOG301368.
    HOGENOMi HOG000093358.
    KOi K11268.
    OMAi ISRIWVC.
    OrthoDBi EOG7D2FPZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30476-MONOMER.

    Miscellaneous databases

    NextBioi 966420.

    Gene expression databases

    Genevestigatori P43605.

    Family and domain databases

    InterProi IPR028005. AcTrfase_ESCO_Znf_dom.
    IPR016181. Acyl_CoA_acyltransferase.
    IPR028009. ESCO_Acetyltransf_dom.
    [Graphical view ]
    Pfami PF13880. Acetyltransf_13. 1 hit.
    PF13878. zf-C2H2_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery."
      Skibbens R.V., Corson L.B., Koshland D., Hieter P.
      Genes Dev. 13:307-319(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
      Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
      Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: ATCC 200060 / W303.
    3. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
      Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion."
      Ivanov D., Schleiffer A., Eisenhaber F., Mechtler K., Haering C.H., Nasmyth K.
      Curr. Biol. 12:323-328(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT LYS-223, ENZYME ACTIVITY, MUTAGENESIS OF GLY-211; 222-ARG-LYS-223; GLY-225 AND ASP-232.
    7. "Mechanical link between cohesion establishment and DNA replication: Ctf7p/Eco1p, a cohesion establishment factor, associates with three different replication factor C complexes."
      Kenna M.A., Skibbens R.V.
      Mol. Cell. Biol. 23:2999-3007(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CHL12; RFC1; RFC2; RFC3; RFC4; RFC5 AND RAD24.
    8. "Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion."
      Skibbens R.V.
      Genetics 166:33-42(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHL1.
    9. "The spindle pole body assembly component Mps3p/Nep98p functions in sister chromatid cohesion."
      Antoniacci L.M., Kenna M.A., Uetz P., Fields S., Skibbens R.V.
      J. Biol. Chem. 279:49542-49550(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MPS3.
    10. "Ctf7p/Eco1p exhibits acetyltransferase activity -- but does it matter?"
      Brands A., Skibbens R.V.
      Curr. Biol. 15:R50-R51(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-35 AND HIS-53.
    11. "Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast."
      Zhang J., Shi X., Li Y., Kim B.J., Jia J., Huang Z., Yang T., Fu X., Jung S.Y., Wang Y., Zhang P., Kim S.T., Pan X., Qin J.
      Mol. Cell 31:143-151(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, AUTOACETYLATION, INTERACTION WITH SMC3.

    Entry informationi

    Entry nameiECO1_YEAST
    AccessioniPrimary (citable) accession number: P43605
    Secondary accession number(s): D6VTQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3