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P43603 (LSB3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LAS seventeen-binding protein 3

Short name=LAS17-binding protein 3
Gene names
Name:LSB3
Ordered Locus Names:YFR024C-A
ORF Names:YFR024C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with LAS17. Ref.7

Subcellular location

Cytoplasm Ref.9.

Post-translational modification

Phosphorylation of Ser-397 is induced 2-fold in response to mating pheromone.

Miscellaneous

Present with 18000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SH3YL1 family.

Contains 1 SH3 domain.

Sequence caution

The sequence BAA09263.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainSH3 domain
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cortical patch localization

Inferred from mutant phenotype PubMed 19158382. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 459459LAS seventeen-binding protein 3
PRO_0000202691

Regions

Domain400 – 45960SH3
Compositional bias229 – 28456Asp-rich

Amino acid modifications

Modified residue2271Phosphoserine Ref.11
Modified residue2981Phosphothreonine Ref.11
Modified residue3001Phosphoserine Ref.4 Ref.5 Ref.11
Modified residue3031Phosphoserine Ref.3 Ref.4 Ref.5 Ref.11
Modified residue3931Phosphothreonine Ref.4 Ref.11
Modified residue3971Phosphoserine Ref.11
Modified residue4021Phosphoserine Ref.4 Ref.11
Modified residue4161Phosphoserine Ref.5

Secondary structure

........... 459
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43603 [UniParc].

Last modified July 22, 2008. Version 3.
Checksum: F486C787B5771AAC

FASTA45949,344
        10         20         30         40         50         60 
MGINNPIPRS LKSETKKAAK ILASFVKPNQ VFGADQVIPP DVLKRAKGLA IITILKAGFL 

        70         80         90        100        110        120 
FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGIEL TDFVFILNTQ DAVKSFSEFG 

       130        140        150        160        170        180 
TITLGGNVSV SAGPLGRSAE AAASASAGGV AAVFAYSKSK GLFAGVSVEG SAIIERREAN 

       190        200        210        220        230        240 
RKFYGDNCTA KMILSGRIRP PPAVDPLFRV LESRAFNYRP SNGGRGSFDD DEDDYYDDDD 

       250        260        270        280        290        300 
YYNDIPSSFS STDASSTRPN TRSTRRRAQS GSRYTFDDDD DDDDYGTGYS RNSRLAPTNS 

       310        320        330        340        350        360 
GGSGGKLDDP SGASSYYASH RRSGTAQSRA RSSRNRWADD EYDDYDDDYE SGYRRGNGRD 

       370        380        390        400        410        420 
RTKDREVDDL SNRFSKSRIS SASTPQTSQG RFTAPTSPST SSPKAVALYS FAGEESGDLP 

       430        440        450 
FRKGDVITIL KKSDSQNDWW TGRVNGREGI FPANYVELV 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[4]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-303; THR-393 AND SER-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-303 AND SER-416, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]Saccharomyces Genome Database (SGD)
Unpublished observations (JUN-2008)
Cited for: REVISION OF GENE MODEL.
[7]"The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LAS17.
[8]"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
Davis C.A., Grate L., Spingola M., Ares M. Jr.
Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF INTRON.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227; THR-298; SER-300; SER-303; THR-393; SER-397 AND SER-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Crystal structure of the SH3 domain from a S.cerevisiae hypothetical 40.4 kDa protein at 1.39 A resolution."
Kursula P., Lehmann F., Song Y.H., Wilmanns M.
Submitted (APR-2004) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 393-451.
[13]"Yeast SH3 domain structural genomics."
Kursula P., Kursula I., Lehmann F., Song Y.H., Wilmanns M.
Submitted (APR-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 393-451.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50617 Genomic DNA. Translation: BAA09263.1. Sequence problems.
BK006940 Genomic DNA. Translation: DAA12464.1.
RefSeqNP_219497.4. NM_001180865.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OOTX-ray1.39A401-459[»]
1SSHX-ray1.40A401-459[»]
ProteinModelPortalP43603.
SMRP43603. Positions 368-459.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31177. 125 interactions.
DIPDIP-2753N.
DIP-6259N.
IntActP43603. 126 interactions.
MINTMINT-374953.
STRING4932.YFR024C-A.

Proteomic databases

PaxDbP43603.
PeptideAtlasP43603.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR024C-A; YFR024C-A; YFR024C-A.
GeneID850580.
KEGGsce:YFR024C-A.

Organism-specific databases

CYGDYFR024c-a.
SGDS000002968. LSB3.

Phylogenomic databases

eggNOGCOG2930.
GeneTreeENSGT00510000048137.
HOGENOMHOG000215564.
OMAHAKGFAI.
OrthoDBEOG7X9GH6.

Enzyme and pathway databases

BioCycYEAST:G3O-30504-MONOMER.

Gene expression databases

GenevestigatorP43603.

Family and domain databases

InterProIPR001452. SH3_domain.
IPR007461. Ysc84_actin-binding.
[Graphical view]
PfamPF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP43603.
NextBio966409.

Entry information

Entry nameLSB3_YEAST
AccessionPrimary (citable) accession number: P43603
Secondary accession number(s): D6VTQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 22, 2008
Last modified: March 19, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references