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P43603

- LSB3_YEAST

UniProt

P43603 - LSB3_YEAST

Protein

LAS seventeen-binding protein 3

Gene

LSB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 3 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. actin cortical patch localization Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30504-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    LAS seventeen-binding protein 3
    Short name:
    LAS17-binding protein 3
    Gene namesi
    Name:LSB3
    Ordered Locus Names:YFR024C-A
    ORF Names:YFR024C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR024c-a.
    SGDiS000002968. LSB3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 459459LAS seventeen-binding protein 3PRO_0000202691Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei227 – 2271Phosphoserine1 Publication
    Modified residuei298 – 2981Phosphothreonine1 Publication
    Modified residuei300 – 3001Phosphoserine3 Publications
    Modified residuei303 – 3031Phosphoserine4 Publications
    Modified residuei393 – 3931Phosphothreonine2 Publications
    Modified residuei397 – 3971Phosphoserine1 Publication
    Modified residuei402 – 4021Phosphoserine2 Publications
    Modified residuei416 – 4161Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation of Ser-397 is induced 2-fold in response to mating pheromone.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43603.
    PaxDbiP43603.
    PeptideAtlasiP43603.

    Expressioni

    Gene expression databases

    GenevestigatoriP43603.

    Interactioni

    Subunit structurei

    Interacts with LAS17.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P158916EBI-22980,EBI-2036
    ACF2Q121687EBI-22980,EBI-32973
    ACF4P471294EBI-22980,EBI-25556
    AGP2P380902EBI-22980,EBI-2362
    AIM21P405632EBI-22980,EBI-25376
    AIM3P382664EBI-22980,EBI-21584
    APP1P539338EBI-22980,EBI-28798
    BSP1Q066047EBI-22980,EBI-37047
    CDC6P091192EBI-22980,EBI-4447
    CUE5Q084127EBI-22980,EBI-37580
    ERT1P381403EBI-22980,EBI-21048
    GTS1P409564EBI-22980,EBI-7968
    GYL1Q043226EBI-22980,EBI-27427
    GYP5Q123443EBI-22980,EBI-38508
    HUA1P403255EBI-22980,EBI-23614
    LAS17Q124469EBI-22980,EBI-10022
    RIM101P334002EBI-22980,EBI-14422
    ROG1P531182EBI-22980,EBI-24005
    SEC8P328553EBI-22980,EBI-16896
    SLA1P327908EBI-22980,EBI-17313
    SLM2P539552EBI-22980,EBI-28706
    STP22P256043EBI-22980,EBI-411625
    UBP7P404532EBI-22980,EBI-19857
    YBP2P531692EBI-22980,EBI-23796
    YDL156WQ125102EBI-22980,EBI-35343
    YHR182WP388702EBI-22980,EBI-24848

    Protein-protein interaction databases

    BioGridi31177. 125 interactions.
    DIPiDIP-2753N.
    DIP-6259N.
    IntActiP43603. 126 interactions.
    MINTiMINT-374953.
    STRINGi4932.YFR024C-A.

    Structurei

    Secondary structure

    1
    459
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi404 – 4096
    Beta strandi426 – 4316
    Beta strandi438 – 4447
    Beta strandi447 – 4526
    Helixi453 – 4553
    Beta strandi456 – 4583

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OOTX-ray1.39A401-459[»]
    1SSHX-ray1.40A401-459[»]
    ProteinModelPortaliP43603.
    SMRiP43603. Positions 368-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43603.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini400 – 45960SH3PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi229 – 28456Asp-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SH3YL1 family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG2930.
    GeneTreeiENSGT00510000048137.
    HOGENOMiHOG000215564.
    OMAiHAKGFAI.
    OrthoDBiEOG7X9GH6.

    Family and domain databases

    InterProiIPR001452. SH3_domain.
    IPR007461. Ysc84_actin-binding.
    [Graphical view]
    PfamiPF04366. DUF500. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43603-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGINNPIPRS LKSETKKAAK ILASFVKPNQ VFGADQVIPP DVLKRAKGLA    50
    IITILKAGFL FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGIEL 100
    TDFVFILNTQ DAVKSFSEFG TITLGGNVSV SAGPLGRSAE AAASASAGGV 150
    AAVFAYSKSK GLFAGVSVEG SAIIERREAN RKFYGDNCTA KMILSGRIRP 200
    PPAVDPLFRV LESRAFNYRP SNGGRGSFDD DEDDYYDDDD YYNDIPSSFS 250
    STDASSTRPN TRSTRRRAQS GSRYTFDDDD DDDDYGTGYS RNSRLAPTNS 300
    GGSGGKLDDP SGASSYYASH RRSGTAQSRA RSSRNRWADD EYDDYDDDYE 350
    SGYRRGNGRD RTKDREVDDL SNRFSKSRIS SASTPQTSQG RFTAPTSPST 400
    SSPKAVALYS FAGEESGDLP FRKGDVITIL KKSDSQNDWW TGRVNGREGI 450
    FPANYVELV 459
    Length:459
    Mass (Da):49,344
    Last modified:July 22, 2008 - v3
    Checksum:iF486C787B5771AAC
    GO

    Sequence cautioni

    The sequence BAA09263.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09263.1. Sequence problems.
    BK006940 Genomic DNA. Translation: DAA12464.1.
    RefSeqiNP_219497.4. NM_001180865.3.

    Genome annotation databases

    EnsemblFungiiYFR024C-A; YFR024C-A; YFR024C-A.
    GeneIDi850580.
    KEGGisce:YFR024C-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09263.1 . Sequence problems.
    BK006940 Genomic DNA. Translation: DAA12464.1 .
    RefSeqi NP_219497.4. NM_001180865.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OOT X-ray 1.39 A 401-459 [» ]
    1SSH X-ray 1.40 A 401-459 [» ]
    ProteinModelPortali P43603.
    SMRi P43603. Positions 368-459.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31177. 125 interactions.
    DIPi DIP-2753N.
    DIP-6259N.
    IntActi P43603. 126 interactions.
    MINTi MINT-374953.
    STRINGi 4932.YFR024C-A.

    Proteomic databases

    MaxQBi P43603.
    PaxDbi P43603.
    PeptideAtlasi P43603.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR024C-A ; YFR024C-A ; YFR024C-A .
    GeneIDi 850580.
    KEGGi sce:YFR024C-A.

    Organism-specific databases

    CYGDi YFR024c-a.
    SGDi S000002968. LSB3.

    Phylogenomic databases

    eggNOGi COG2930.
    GeneTreei ENSGT00510000048137.
    HOGENOMi HOG000215564.
    OMAi HAKGFAI.
    OrthoDBi EOG7X9GH6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30504-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P43603.
    NextBioi 966409.

    Gene expression databases

    Genevestigatori P43603.

    Family and domain databases

    InterProi IPR001452. SH3_domain.
    IPR007461. Ysc84_actin-binding.
    [Graphical view ]
    Pfami PF04366. DUF500. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-303; THR-393 AND SER-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-303 AND SER-416, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Saccharomyces Genome Database (SGD)
      Unpublished observations (JUN-2008)
      Cited for: REVISION OF GENE MODEL.
    7. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
      Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
      Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LAS17.
    8. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
      Davis C.A., Grate L., Spingola M., Ares M. Jr.
      Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INTRON.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227; THR-298; SER-300; SER-303; THR-393; SER-397 AND SER-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Crystal structure of the SH3 domain from a S.cerevisiae hypothetical 40.4 kDa protein at 1.39 A resolution."
      Kursula P., Lehmann F., Song Y.H., Wilmanns M.
      Submitted (APR-2004) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 393-451.
    13. "Yeast SH3 domain structural genomics."
      Kursula P., Kursula I., Lehmann F., Song Y.H., Wilmanns M.
      Submitted (APR-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 393-451.

    Entry informationi

    Entry nameiLSB3_YEAST
    AccessioniPrimary (citable) accession number: P43603
    Secondary accession number(s): D6VTQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 125 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 18000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3