Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P43603

- LSB3_YEAST

UniProt

P43603 - LSB3_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

LAS seventeen-binding protein 3

Gene

LSB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. actin cortical patch localization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30504-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
LAS seventeen-binding protein 3
Short name:
LAS17-binding protein 3
Gene namesi
Name:LSB3
Ordered Locus Names:YFR024C-A
ORF Names:YFR024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR024c-a.
SGDiS000002968. LSB3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459LAS seventeen-binding protein 3PRO_0000202691Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei227 – 2271Phosphoserine1 Publication
Modified residuei298 – 2981Phosphothreonine1 Publication
Modified residuei300 – 3001Phosphoserine3 Publications
Modified residuei303 – 3031Phosphoserine4 Publications
Modified residuei393 – 3931Phosphothreonine2 Publications
Modified residuei397 – 3971Phosphoserine1 Publication
Modified residuei402 – 4021Phosphoserine2 Publications
Modified residuei416 – 4161Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation of Ser-397 is induced 2-fold in response to mating pheromone.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43603.
PaxDbiP43603.
PeptideAtlasiP43603.

Expressioni

Gene expression databases

GenevestigatoriP43603.

Interactioni

Subunit structurei

Interacts with LAS17.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABP1P158916EBI-22980,EBI-2036
ACF2Q121687EBI-22980,EBI-32973
ACF4P471294EBI-22980,EBI-25556
AGP2P380902EBI-22980,EBI-2362
AIM21P405632EBI-22980,EBI-25376
AIM3P382664EBI-22980,EBI-21584
APP1P539338EBI-22980,EBI-28798
BSP1Q066047EBI-22980,EBI-37047
CDC6P091192EBI-22980,EBI-4447
CUE5Q084127EBI-22980,EBI-37580
ERT1P381403EBI-22980,EBI-21048
GTS1P409564EBI-22980,EBI-7968
GYL1Q043226EBI-22980,EBI-27427
GYP5Q123443EBI-22980,EBI-38508
HUA1P403255EBI-22980,EBI-23614
LAS17Q124469EBI-22980,EBI-10022
RIM101P334002EBI-22980,EBI-14422
ROG1P531182EBI-22980,EBI-24005
SEC8P328553EBI-22980,EBI-16896
SLA1P327908EBI-22980,EBI-17313
SLM2P539552EBI-22980,EBI-28706
STP22P256043EBI-22980,EBI-411625
UBP7P404532EBI-22980,EBI-19857
YBP2P531692EBI-22980,EBI-23796
YDL156WQ125102EBI-22980,EBI-35343
YHR182WP388702EBI-22980,EBI-24848

Protein-protein interaction databases

BioGridi31177. 126 interactions.
DIPiDIP-2753N.
DIP-6259N.
IntActiP43603. 126 interactions.
MINTiMINT-374953.
STRINGi4932.YFR024C-A.

Structurei

Secondary structure

1
459
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi404 – 4096
Beta strandi426 – 4316
Beta strandi438 – 4447
Beta strandi447 – 4526
Helixi453 – 4553
Beta strandi456 – 4583

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OOTX-ray1.39A401-459[»]
1SSHX-ray1.40A401-459[»]
ProteinModelPortaliP43603.
SMRiP43603. Positions 368-459.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43603.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini400 – 45960SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 28456Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the SH3YL1 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG2930.
GeneTreeiENSGT00510000048137.
HOGENOMiHOG000215564.
InParanoidiP43603.
OMAiHAKGFAI.
OrthoDBiEOG7X9GH6.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR007461. Ysc84_actin-binding.
[Graphical view]
PfamiPF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43603-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGINNPIPRS LKSETKKAAK ILASFVKPNQ VFGADQVIPP DVLKRAKGLA
60 70 80 90 100
IITILKAGFL FSGRAGSGVI VARLKDGTWS APSAIAMAGA GAGGMVGIEL
110 120 130 140 150
TDFVFILNTQ DAVKSFSEFG TITLGGNVSV SAGPLGRSAE AAASASAGGV
160 170 180 190 200
AAVFAYSKSK GLFAGVSVEG SAIIERREAN RKFYGDNCTA KMILSGRIRP
210 220 230 240 250
PPAVDPLFRV LESRAFNYRP SNGGRGSFDD DEDDYYDDDD YYNDIPSSFS
260 270 280 290 300
STDASSTRPN TRSTRRRAQS GSRYTFDDDD DDDDYGTGYS RNSRLAPTNS
310 320 330 340 350
GGSGGKLDDP SGASSYYASH RRSGTAQSRA RSSRNRWADD EYDDYDDDYE
360 370 380 390 400
SGYRRGNGRD RTKDREVDDL SNRFSKSRIS SASTPQTSQG RFTAPTSPST
410 420 430 440 450
SSPKAVALYS FAGEESGDLP FRKGDVITIL KKSDSQNDWW TGRVNGREGI

FPANYVELV
Length:459
Mass (Da):49,344
Last modified:July 22, 2008 - v3
Checksum:iF486C787B5771AAC
GO

Sequence cautioni

The sequence BAA09263.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09263.1. Sequence problems.
BK006940 Genomic DNA. Translation: DAA12464.1.
RefSeqiNP_219497.4. NM_001180865.3.

Genome annotation databases

EnsemblFungiiYFR024C-A; YFR024C-A; YFR024C-A.
GeneIDi850580.
KEGGisce:YFR024C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09263.1 . Sequence problems.
BK006940 Genomic DNA. Translation: DAA12464.1 .
RefSeqi NP_219497.4. NM_001180865.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OOT X-ray 1.39 A 401-459 [» ]
1SSH X-ray 1.40 A 401-459 [» ]
ProteinModelPortali P43603.
SMRi P43603. Positions 368-459.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31177. 126 interactions.
DIPi DIP-2753N.
DIP-6259N.
IntActi P43603. 126 interactions.
MINTi MINT-374953.
STRINGi 4932.YFR024C-A.

Proteomic databases

MaxQBi P43603.
PaxDbi P43603.
PeptideAtlasi P43603.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR024C-A ; YFR024C-A ; YFR024C-A .
GeneIDi 850580.
KEGGi sce:YFR024C-A.

Organism-specific databases

CYGDi YFR024c-a.
SGDi S000002968. LSB3.

Phylogenomic databases

eggNOGi COG2930.
GeneTreei ENSGT00510000048137.
HOGENOMi HOG000215564.
InParanoidi P43603.
OMAi HAKGFAI.
OrthoDBi EOG7X9GH6.

Enzyme and pathway databases

BioCyci YEAST:G3O-30504-MONOMER.

Miscellaneous databases

EvolutionaryTracei P43603.
NextBioi 966409.

Gene expression databases

Genevestigatori P43603.

Family and domain databases

InterProi IPR001452. SH3_domain.
IPR007461. Ysc84_actin-binding.
[Graphical view ]
Pfami PF04366. DUF500. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-303; THR-393 AND SER-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300; SER-303 AND SER-416, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Saccharomyces Genome Database (SGD)
    Unpublished observations (JUN-2008)
    Cited for: REVISION OF GENE MODEL.
  7. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
    Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
    Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAS17.
  8. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
    Davis C.A., Grate L., Spingola M., Ares M. Jr.
    Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227; THR-298; SER-300; SER-303; THR-393; SER-397 AND SER-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Crystal structure of the SH3 domain from a S.cerevisiae hypothetical 40.4 kDa protein at 1.39 A resolution."
    Kursula P., Lehmann F., Song Y.H., Wilmanns M.
    Submitted (APR-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 393-451.
  13. "Yeast SH3 domain structural genomics."
    Kursula P., Kursula I., Lehmann F., Song Y.H., Wilmanns M.
    Submitted (APR-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 393-451.

Entry informationi

Entry nameiLSB3_YEAST
AccessioniPrimary (citable) accession number: P43603
Secondary accession number(s): D6VTQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 22, 2008
Last modified: October 29, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3