Autophagy-related protein 18 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P43601 (ATG18_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Autophagy-related protein 18

Alternative name(s):
Cytoplasm to vacuole targeting protein 18
Needed for premeiotic replication protein 1
Swollen vacuole phenotype protein 1

Gene names

Name:	ATG18
Synonyms:	AUT10, CVT18, NMR1, SVP1
Ordered Locus Names:	YFR021W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). May negatively regulate FAB1 activity by sequestering or masking VAC7 from FAB1. Necessary for proper vacuole morphology. Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and autophagy. Involved in correct ATG9 trafficking to the pre-autophagosomal structure. Might also be involved in premeiotic DNA replication. Ref.3 Ref.4 Ref.5 Ref.8 Ref.10 Ref.11
Subunit structure	Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. Interacts with ATG9 and VAC17. Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Preautophagosomal structure membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Note: Peripheral membrane protein of pre-autophagosomal structure (PAS) and vacuole. Requires VAC7 pour vacuole membrane localization. Ref.8 Ref.9 Ref.10 Ref.11
Domain	The 377 first amino acids might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane.
Miscellaneous	Present with 1560 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat SVP1 family. Contains 2 WD repeats.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Membrane Vacuole
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CVT pathway Inferred from direct assay Ref.4. Source: SGD late endosome to vacuole transport Inferred from mutant phenotype Ref.9. Source: SGD macroautophagy Inferred from direct assay Ref.4. Source: SGD peroxisome degradation Inferred from mutant phenotype Ref.5. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype PubMed 18701704. Source: SGD vacuolar protein processing Inferred from direct assay Ref.4. Source: SGD
Cellular_component	PAS complex Inferred from direct assay Ref.11. Source: SGD cytosol Inferred from direct assay Ref.4. Source: SGD endosome Inferred from direct assay PubMed 18769150. Source: SGD fungal-type vacuole membrane Inferred from direct assay Ref.9 PubMed 22842922. Source: SGD pre-autophagosomal structure Inferred from direct assay PubMed 18497569. Source: SGD pre-autophagosomal structure membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidylinositol-3,5-bisphosphate binding Inferred from Biological aspect of Ancestor. Source: RefGenome ubiquitin binding Inferred from direct assay PubMed 21070969. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 500

500

Autophagy-related protein 18

PRO_0000050874

Regions

Repeat

243 – 283

WD 1

Repeat

288 – 327

WD 2

Region

284 – 287

Necessary for proper localization to vacuole membrane

Amino acid modifications

Modified residue

354

Phosphoserine Ref.12

Experimental info

Mutagenesis

73 – 76

RRLR → SSLS: Slight reduction of PIP2 binding. Ref.9

Mutagenesis

285 – 286

RR → GG: Loss of recruitment to vacuole membrane. Ref.10

Mutagenesis

285 – 286

RR → TT: 40-fold decrease of affinity to PIP2. Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P43601 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 06B2DFAF842AE933
Show »

FASTA

500

55,102

        10         20         30         40         50         60 
MSDSSPTINF INFNQTGTCI SLGTSKGFKI FNCEPFGKFY SEDSGGYAIV EMLFSTSLLA 

        70         80         90        100        110        120 
LVGIGDQPAL SPRRLRIINT KKHSIICEVT FPTSILSVKM NKSRLVVLLQ EQIYIYDINT 

       130        140        150        160        170        180 
MRLLHTIETN PNPRGLMAMS PSVANSYLVY PSPPKVINSE IKAHATTNNI TLSVGGNTET 

       190        200        210        220        230        240 
SFKRDQQDAG HSDISDLDQY SSFTKRDDAD PTSSNGGNSS IIKNGDVIVF NLETLQPTMV 

       250        260        270        280        290        300 
IEAHKGEIAA MAISFDGTLM ATASDKGTII RVFDIETGDK IYQFRRGTYA TRIYSISFSE 

       310        320        330        340        350        360 
DSQYLAVTGS SKTVHIFKLG HSMSNNKLDS DDSNMEEAAA DDSSLDTTSI DALSDEENPT 

       370        380        390        400        410        420 
RLAREPYVDA SRKTMGRMIR YSSQKLSRRA ARTLGQIFPI KVTSLLESSR HFASLKLPVE 

       430        440        450        460        470        480 
TNSHVMTISS IGSPIDIDTS EYPELFETGN SASTESYHEP VMKMVPIRVV SSDGYLYNFV 

       490        500 
MDPERGGDCL ILSQYSILMD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Autophagy and the cytoplasm to vacuole targeting pathway both require Aut10p." Barth H., Meiling-Wesse K., Epple U.D., Thumm M. FEBS Lett. 508:23-28(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris." Guan J., Stromhaug P.E., George M.D., Habibzadegah-Tari P., Bevan A., Dunn W.A. Jr., Klionsky D.J. Mol. Biol. Cell 12:3821-3838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure." Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J. Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG9.
[9]	"Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors." Dove S.K., Piper R.C., McEwen R.K., Yu J.W., King M.C., Hughes D.C., Thuring J., Holmes A.B., Cooke F.T., Michell R.H., Parker P.J., Lemmon M.A. EMBO J. 23:1922-1933(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PIP2, SUBCELLULAR LOCATION, MUTAGENESIS OF 73-ARG--ARG-76 AND 285-ARG-ARG-286.
[10]	"Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate." Efe J.A., Botelho R.J., Emr S.D. Mol. Biol. Cell 18:4232-4244(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VAC17, MUTAGENESIS OF 285-ARG--ARG-286.
[11]	"VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse." Jin N., Chow C.Y., Liu L., Zolov S.N., Bronson R., Davisson M., Petersen J.L., Zhang Y., Park S., Duex J.E., Goldowitz D., Meisler M.H., Weisman L.S. EMBO J. 27:3221-3234(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE PI(3,5)P2 REGULATORY COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09260.1. BK006940 Genomic DNA. Translation: DAA12461.1.
PIR	S56276.
RefSeq	NP_444297.1. NM_001179986.1.
3D structure databases
ProteinModelPortal	P43601.
SMR	P43601. Positions 5-33, 231-331, 455-485.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5185N.
IntAct	P43601. 15 interactions.
MINT	MINT-541235.
STRING	4932.YFR021W.
Proteomic databases
PaxDb	P43601.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR021W; YFR021W; YFR021W.
GeneID	850577.
KEGG	sce:YFR021W.
Organism-specific databases
CYGD	YFR021w.
SGD	S000001917. ATG18.
Phylogenomic databases
eggNOG	NOG317564.
GeneTree	ENSGT00530000062939.
HOGENOM	HOG000217543.
OMA	RGGDCIL.
OrthoDB	EOG4M0J97.
Gene expression databases
Genevestigator	P43601.
GermOnline	YFR021W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 2 hits. [Graphical view]
SMART	SM00320. WD40. 2 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966400.

Entry information

Entry name

ATG18_YEAST

Accession

Primary (citable) accession number: P43601
Secondary accession number(s): D6VTQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents