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Protein

Autophagy-related protein 18

Gene

ATG18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). May negatively regulate FAB1 activity by sequestering or masking VAC7 from FAB1. Necessary for proper vacuole morphology. Plays an important role in osmotically-induced vacuole fragmentation. Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and starvation-induced autophagy. Involved in correct ATG9 trafficking to the pre-autophagosomal structure. Might also be involved in premeiotic DNA replication. With ATG2, protects ATG8 from ATG4-mediated cleavage.17 Publications

GO - Molecular functioni

  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: GO_Central
  • ubiquitin binding Source: SGD

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • CVT pathway Source: SGD
  • late endosome to vacuole transport Source: SGD
  • late nucleophagy Source: SGD
  • macroautophagy Source: SGD
  • mitophagy Source: GO_Central
  • pexophagy Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • protein lipidation Source: GO_Central
  • protein localization to pre-autophagosomal structure Source: GO_Central
  • vacuolar protein processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30472-MONOMER.
ReactomeiR-SCE-1632852. Macroautophagy.

Protein family/group databases

TCDBi9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 181 Publication
Alternative name(s):
Cytoplasm to vacuole targeting protein 181 Publication
Needed for premeiotic replication protein 11 Publication
Swollen vacuole phenotype protein 11 Publication
Gene namesi
Name:ATG181 Publication
Synonyms:AUT101 Publication, CVT181 Publication, NMR11 Publication, SVP11 Publication
Ordered Locus Names:YFR021WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR021W.
SGDiS000001917. ATG18.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • endosome Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • fungal-type vacuole membrane Source: SGD
  • PAS complex Source: SGD
  • pre-autophagosomal structure Source: SGD
  • pre-autophagosomal structure membrane Source: GO_Central
  • vacuolar membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73 – 76RRLR → SSLS: Slight reduction of PIP2 binding. 4
Mutagenesisi264S → A: Impairs membrane-association. 1 Publication1
Mutagenesisi268T → A: Impairs membrane-association. 1 Publication1
Mutagenesisi271R → A: Impairs membrane-association. 1 Publication1
Mutagenesisi285 – 286RR → GG: Loss of recruitment to vacuole membrane. 1 Publication2
Mutagenesisi285 – 286RR → TT: 40-fold decrease of affinity to PIP2. 1 Publication2
Mutagenesisi285R → A: Impairs membrane-association. 1 Publication1
Mutagenesisi286R → A: Impairs membrane-association. 1 Publication1
Mutagenesisi311S → A: Impairs membrane-association. 1 Publication1
Mutagenesisi313T → A: Impairs membrane-association. 1 Publication1
Mutagenesisi315H → A: Impairs membrane-association. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508741 – 500Autophagy-related protein 18Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43601.
PRIDEiP43601.

PTM databases

iPTMnetiP43601.

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. Interacts with ATG2, ATG9 and VAC17. The ATG2-ATG18 complex is essential for autophagosome formation.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG2P538555EBI-22968,EBI-29212
VAC14Q067085EBI-22968,EBI-27189

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi31174. 81 interactors.
DIPiDIP-5185N.
IntActiP43601. 16 interactors.
MINTiMINT-541235.

Structurei

3D structure databases

ProteinModelPortaliP43601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati243 – 283WD 1Add BLAST41
Repeati288 – 327WD 2Add BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni284 – 287Necessary for proper localization to vacuole membrane4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi284 – 288FRRGT-motif1 Publication5

Domaini

The 377 first amino acids might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane. Association to the membrane can also occur through binding to phosphatidylinositol 3-monophosphate (PI3P).1 Publication
The FRRGT-motif is essential for the cytoplasm to vacuole transport (Cvt) pathway, for the recruitment of ATG8 and ATG16 to the PAS in nutrient-rich medium, and for its recruitment to and dissociation from the PAS under starvation conditions.1 Publication

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated
Contains 2 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217543.
InParanoidiP43601.
KOiK17908.
OMAiCEVTFPT.
OrthoDBiEOG092C3UKJ.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSSPTINF INFNQTGTCI SLGTSKGFKI FNCEPFGKFY SEDSGGYAIV
60 70 80 90 100
EMLFSTSLLA LVGIGDQPAL SPRRLRIINT KKHSIICEVT FPTSILSVKM
110 120 130 140 150
NKSRLVVLLQ EQIYIYDINT MRLLHTIETN PNPRGLMAMS PSVANSYLVY
160 170 180 190 200
PSPPKVINSE IKAHATTNNI TLSVGGNTET SFKRDQQDAG HSDISDLDQY
210 220 230 240 250
SSFTKRDDAD PTSSNGGNSS IIKNGDVIVF NLETLQPTMV IEAHKGEIAA
260 270 280 290 300
MAISFDGTLM ATASDKGTII RVFDIETGDK IYQFRRGTYA TRIYSISFSE
310 320 330 340 350
DSQYLAVTGS SKTVHIFKLG HSMSNNKLDS DDSNMEEAAA DDSSLDTTSI
360 370 380 390 400
DALSDEENPT RLAREPYVDA SRKTMGRMIR YSSQKLSRRA ARTLGQIFPI
410 420 430 440 450
KVTSLLESSR HFASLKLPVE TNSHVMTISS IGSPIDIDTS EYPELFETGN
460 470 480 490 500
SASTESYHEP VMKMVPIRVV SSDGYLYNFV MDPERGGDCL ILSQYSILMD
Length:500
Mass (Da):55,102
Last modified:November 1, 1995 - v1
Checksum:i06B2DFAF842AE933
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09260.1.
BK006940 Genomic DNA. Translation: DAA12461.1.
PIRiS56276.
RefSeqiNP_444297.1. NM_001179986.1.

Genome annotation databases

EnsemblFungiiBAA09260; BAA09260; BAA09260.
YFR021W; YFR021W; YFR021W.
GeneIDi850577.
KEGGisce:YFR021W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09260.1.
BK006940 Genomic DNA. Translation: DAA12461.1.
PIRiS56276.
RefSeqiNP_444297.1. NM_001179986.1.

3D structure databases

ProteinModelPortaliP43601.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31174. 81 interactors.
DIPiDIP-5185N.
IntActiP43601. 16 interactors.
MINTiMINT-541235.

Protein family/group databases

TCDBi9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

PTM databases

iPTMnetiP43601.

Proteomic databases

MaxQBiP43601.
PRIDEiP43601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09260; BAA09260; BAA09260.
YFR021W; YFR021W; YFR021W.
GeneIDi850577.
KEGGisce:YFR021W.

Organism-specific databases

EuPathDBiFungiDB:YFR021W.
SGDiS000001917. ATG18.

Phylogenomic databases

GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217543.
InParanoidiP43601.
KOiK17908.
OMAiCEVTFPT.
OrthoDBiEOG092C3UKJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30472-MONOMER.
ReactomeiR-SCE-1632852. Macroautophagy.

Miscellaneous databases

PROiP43601.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATG18_YEAST
AccessioniPrimary (citable) accession number: P43601
Secondary accession number(s): D6VTQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1560 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.