Reviewed, UniProtKB/Swiss-Prot P43598 (YFI7_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein YFR017C

Gene names

Ordered Locus Names:

YFR017C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous

Present with 639 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GDB1	Q06625	1	EBI-22956,EBI-37861

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Uncharacterized protein YFR017C		PRO_0000202687
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.7
	Modified residue	13	1	Phosphothreonine Ref.7
	Modified residue	14	1	Phosphoserine Ref.7
	Modified residue	64	1	Phosphoserine Ref.7 Ref.3 Ref.6
	Modified residue	65	1	Phosphothreonine Ref.7 Ref.6
	Modified residue	83	1	Phosphoserine Ref.7 Ref.6
	Modified residue	95	1	Phosphoserine Ref.7
	Modified residue	96	1	Phosphoserine Ref.7 Ref.3 Ref.6
	Modified residue	164	1	Phosphoserine Ref.7
	Modified residue	175	1	Phosphoserine Ref.7 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P43598-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 72A5C94EB59B4AB1	FASTA	195	21,783
	10 20 30 40 50 60 MTDPHLNTPQ VSTSPTFERS QDFLNIDEPP CAQETPSVST FNLPGPSAPA QSVDKPVPMI 70 80 90 100 110 120 RRRSTNYMDA LNSREQARER ESSIGEHAPG AERRSSGPMD FQNTIHNMQY RTTNDSDLSH 130 140 150 160 170 180 AGVDMGDSIS HTPICSRAGN RPIFKNSYLD NNSNGNSARV PHGSPPQLGT RRKSSFKYED 190 FKKDIYNQLH MFGEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64 AND SER-96, MASS SPECTROMETRY.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; THR-65; SER-83 AND SER-96, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; THR-13; SER-14; SER-64; THR-65; SER-83; SER-95; SER-96; SER-164 AND SER-175, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09256.1. AY558470 Genomic DNA. Translation: AAS56796.1.
PIR	S43032.
RefSeq	NP_116672.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3799N.
IntAct	P43598. 6 interactions.
Proteomic databases
PeptideAtlas	P43598.
Genome annotation databases
Ensembl	YFR017C. Saccharomyces cerevisiae. [Contig view]
GeneID	850572.
GenomeReviews	Gene locus YFR017C in contig D50617_GR.
KEGG	sce:YFR017C.
NMPDR	fig|4932.3.peg.2306.
Organism-specific databases
CYGD	YFR017c.
SGD	S000001913. YFR017C.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43598.
Gene expression databases
GermOnline	YFR017C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	966389.

Entry information

Entry name

YFI7_YEAST

Accession

Primary (citable) accession number: P43598

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)