Ubiquitin carboxyl-terminal hydrolase 6 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P43593 (UBP6_YEAST)

Last modified November 24, 2009. Version 91. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 6
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 6
    Ubiquitin-specific-processing protease 6
    Deubiquitinating enzyme 6

Gene names

Name:	UBP6
Ordered Locus Names:	YFR010W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Ubiquitin C-terminal thioester + H₂O = ubiquitin + a thiol.
Miscellaneous	Present with 6770 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the peptidase C19 family.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein deubiquitination Inferred from mutant phenotype. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	proteasome regulatory particle Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC ubiquitin-specific protease activity Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
	Q02895	1	EBI-19852,EBI-35030
ERF2	Q06551	1	EBI-19852,EBI-37230
ITC1	P53125	1	EBI-19852,EBI-23967
MTM1	P53320	1	EBI-19852,EBI-23594
TIM13	P53299	1	EBI-19852,EBI-9121
YHM1	P38988	1	EBI-19852,EBI-24559

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 499

499

Ubiquitin carboxyl-terminal hydrolase 6

PRO_0000080591

Sites

Active site

118

By similarity

Active site

438

By similarity

Active site

447

By similarity

Amino acid modifications

Modified residue

298

Phosphoserine Ref.5

Modified residue

383

Phosphoserine Ref.5 Ref.4

Modified residue

389

Phosphothreonine Ref.5 Ref.4 Ref.3

Modified residue

470

Phosphoserine Ref.5 Ref.4

Secondary structure

499

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P43593-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: DAE54D1F8AC9960C
Show »

FASTA

499

57,111

        10         20         30         40         50         60 
MSGETFEFNI RHSGKVYPIT LSTDATSADL KSKAEELTQV PSARQKYMVK GGLSGEESIK 

        70         80         90        100        110        120 
IYPLIKPGST VMLLGTPDAN LISKPAKKNN FIEDLAPEQQ VQQFAQLPVG FKNMGNTCYL 

       130        140        150        160        170        180 
NATLQALYRV NDLRDMILNY NPSQGVSNSG AQDEEIHKQI VIEMKRCFEN LQNKSFKSVL 

       190        200        210        220        230        240 
PIVLLNTLRK CYPQFAERDS QGGFYKQQDA EELFTQLFHS MSIVFGDKFS EDFRIQFKTT 

       250        260        270        280        290        300 
IKDTANDNDI TVKENESDSK LQCHISGTTN FMRNGLLEGL NEKIEKRSDL TGANSIYSVE 

       310        320        330        340        350        360 
KKISRLPKFL TVQYVRFFWK RSTNKKSKIL RKVVFPFQLD VADMLTPEYA AEKVKVRDEL 

       370        380        390        400        410        420 
RKVEKEKNEK EREIKRRKFD PSSSENVMTP REQYETQVAL NESEKDQWLE EYKKHFPPNL 

       430        440        450        460        470        480 
EKGENPSCVY NLIGVITHQG ANSESGHYQA FIRDELDENK WYKFNDDKVS VVEKEKIESL 

       490 
AGGGESDSAL ILMYKGFGL

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References

[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-389, MASS SPECTROMETRY.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383; THR-389 AND SER-470, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298; SER-383; THR-389 AND SER-470, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

D50617 Genomic DNA. Translation: BAA09249.1.

PIR

S56265.

RefSeq

NP_116665.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VJV	X-ray	1.74	A	97-499	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6731N.

IntAct

P43593. 65 interactions.

STRING

P43593.

Protein family/group databases

MEROPS

C19.079.

Proteomic databases

PeptideAtlas

P43593.

PRIDE

P43593.

Genome annotation databases

Ensembl

YFR010W; YFR010W; YFR010W; Saccharomyces cerevisiae. [Genome view]

GeneID

850562.

KEGG

sce:YFR010W.

NMPDR

fig|4932.3.peg.2297.

Organism-specific databases

CYGD

YFR010w.

SGD

S000001906. UBP6.

Phylogenomic databases

HOGENOM

P43593.

OMA

ITHKGRS

OrthoDB

EOG9ZPGC5

Enzyme and pathway databases

BRENDA

3.1.2.15. 250.

Gene expression databases

ArrayExpress

P43593.

Genevestigator

P43593.

GermOnline

YFR010W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19.
IPR000626. Ubiquitin.
[Graphical view]

Pfam

PF00443. UCH. 1 hit.
[Graphical view]

SMART

SM00213. UBQ. 1 hit.
[Graphical view]

PROSITE

PS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

966361.

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Entry information

Entry name

UBP6_YEAST

Accession

Primary (citable) accession number: P43593

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents