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Protein

ATP-dependent kinase YFH7

Gene

YFH7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent kinase that could be involved in endoplasmic reticulum membrane assembly.1 Publication

Kineticsi

  1. KM=1178 µM for ATP1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPBy similarity

    GO - Molecular functioni

    • ATPase activity Source: SGD
    • ATP binding Source: UniProtKB-KW
    • kinase activity Source: SGD

    GO - Biological processi

    • phosphorylation Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30460-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent kinase YFH7 (EC:2.7.1.-)
    Alternative name(s):
    Altered inheritance of mitochondria protein 12
    Gene namesi
    Name:YFH7
    Synonyms:AIM12
    Ordered Locus Names:YFR007W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VI

    Organism-specific databases

    EuPathDBiFungiDB:YFR007W.
    SGDiS000001903. YFH7.

    Pathology & Biotechi

    Disruption phenotypei

    Increases frequency of mitochondrial genome loss.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 353353ATP-dependent kinase YFH7PRO_0000202683Add
    BLAST

    Proteomic databases

    MaxQBiP43591.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YBR056WP380811EBI-22928,EBI-21453

    Protein-protein interaction databases

    BioGridi31156. 7 interactions.
    DIPiDIP-5443N.
    IntActiP43591. 2 interactions.
    MINTiMINT-518359.

    Structurei

    Secondary structure

    1
    353
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1714Combined sources
    Turni18 – 214Combined sources
    Beta strandi25 – 306Combined sources
    Helixi37 – 5822Combined sources
    Beta strandi79 – 813Combined sources
    Helixi84 – 929Combined sources
    Turni93 – 953Combined sources
    Helixi98 – 1003Combined sources
    Beta strandi109 – 1135Combined sources
    Beta strandi121 – 1255Combined sources
    Helixi129 – 1324Combined sources
    Beta strandi134 – 1363Combined sources
    Beta strandi155 – 1606Combined sources
    Helixi161 – 1644Combined sources
    Helixi168 – 1714Combined sources
    Beta strandi174 – 1763Combined sources
    Helixi178 – 1814Combined sources
    Turni182 – 1854Combined sources
    Helixi187 – 1893Combined sources
    Helixi192 – 20615Combined sources
    Helixi222 – 2276Combined sources
    Beta strandi237 – 2437Combined sources
    Turni244 – 2474Combined sources
    Beta strandi248 – 2569Combined sources
    Beta strandi262 – 2698Combined sources
    Helixi275 – 28511Combined sources
    Turni286 – 2883Combined sources
    Beta strandi290 – 2967Combined sources
    Helixi299 – 31214Combined sources
    Helixi319 – 32810Combined sources
    Turni330 – 3323Combined sources
    Helixi335 – 3395Combined sources
    Beta strandi347 – 3515Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GA8X-ray1.77A1-353[»]
    2GAAX-ray1.95A1-353[»]
    ProteinModelPortaliP43591.
    SMRiP43591. Positions 2-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43591.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the YFH7 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000141840.
    InParanoidiP43591.
    OMAiILEGLYL.
    OrthoDBiEOG74J9JP.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR011704. ATPase_dyneun-rel_AAA.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF07728. AAA_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P43591-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVDTHKLADD VLQLLDNRIE DNYRVCVILV GSPGSGKSTI AEELCQIINE
    60 70 80 90 100
    KYHTFLSEHP NVIEVNDRLK PMVNLVDSLK TLQPNKVAEM IENQGLFKDH
    110 120 130 140 150
    VEDVNFQPVK YSALTSNNEE CTAVVARGGT ANAIRIAAVD NPVNVNKLAQ
    160 170 180 190 200
    DSINIAQIVP MDGFHLSRRC LDLFKDPQTA HKRRGSPSTF DSNNFLQLCK
    210 220 230 240 250
    ILAKTSLCKV SSHHKFYSTS SVFEKLSKTF SQTIPDIFVP GFNHALKDPT
    260 270 280 290 300
    PDQYCISKFT RIVILEGLYL LYDQENWKKI YKTLADTGAL LVYKIDIDYE
    310 320 330 340 350
    ATEERVAKRH LQSGLVTTIA EGREKFRSND LLNGRDIDNH LIKVDNIVHI

    RND
    Length:353
    Mass (Da):39,885
    Last modified:November 1, 1995 - v1
    Checksum:i521A76D6E9C9F11D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50617 Genomic DNA. Translation: BAA09246.1.
    AY558494 Genomic DNA. Translation: AAS56820.1.
    BK006940 Genomic DNA. Translation: DAA12447.1.
    PIRiS56262.
    RefSeqiNP_116662.1. NM_001179972.1.

    Genome annotation databases

    EnsemblFungiiBAA09246; BAA09246; BAA09246.
    YFR007W; YFR007W; YFR007W.
    GeneIDi850558.
    KEGGisce:YFR007W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50617 Genomic DNA. Translation: BAA09246.1.
    AY558494 Genomic DNA. Translation: AAS56820.1.
    BK006940 Genomic DNA. Translation: DAA12447.1.
    PIRiS56262.
    RefSeqiNP_116662.1. NM_001179972.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GA8X-ray1.77A1-353[»]
    2GAAX-ray1.95A1-353[»]
    ProteinModelPortaliP43591.
    SMRiP43591. Positions 2-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31156. 7 interactions.
    DIPiDIP-5443N.
    IntActiP43591. 2 interactions.
    MINTiMINT-518359.

    Proteomic databases

    MaxQBiP43591.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiBAA09246; BAA09246; BAA09246.
    YFR007W; YFR007W; YFR007W.
    GeneIDi850558.
    KEGGisce:YFR007W.

    Organism-specific databases

    EuPathDBiFungiDB:YFR007W.
    SGDiS000001903. YFH7.

    Phylogenomic databases

    HOGENOMiHOG000141840.
    InParanoidiP43591.
    OMAiILEGLYL.
    OrthoDBiEOG74J9JP.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30460-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP43591.
    PROiP43591.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR011704. ATPase_dyneun-rel_AAA.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF07728. AAA_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Parallel analysis of tagged deletion mutants efficiently identifies genes involved in endoplasmic reticulum biogenesis."
      Wright R., Parrish M.L., Cadera E., Larson L., Matson C.K., Garrett-Engele P., Armour C., Lum P.Y., Shoemaker D.D.
      Yeast 20:881-892(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis."
      Hess D.C., Myers C.L., Huttenhower C., Hibbs M.A., Hayes A.P., Paw J., Clore J.J., Mendoza R.M., Luis B.S., Nislow C., Giaever G., Costanzo M., Troyanskaya O.G., Caudy A.A.
      PLoS Genet. 5:E1000407-E1000407(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Crystal structure and functional analysis identify the P-loop containing protein YFH7 of Saccharomyces cerevisiae as an ATP-dependent kinase."
      Gueguen-Chaignon V., Chaptal V., Lariviere L., Costa N., Lopes P., Morera S., Nessler S.
      Proteins 71:804-812(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS), BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiYFH7_YEAST
    AccessioniPrimary (citable) accession number: P43591
    Secondary accession number(s): D6VTN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: June 8, 2016
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 161 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.