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Protein

Uncharacterized peptidase YFR006W

Gene

YFR006W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Mn2+CuratedNote: Binds 2 manganese ions per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi316 – 3161Manganese 2Sequence analysis
Metal bindingi327 – 3271Manganese 1Sequence analysis
Metal bindingi327 – 3271Manganese 2Sequence analysis
Metal bindingi412 – 4121Manganese 1Sequence analysis
Metal bindingi452 – 4521Manganese 1Sequence analysis
Metal bindingi493 – 4931Manganese 1Sequence analysis
Metal bindingi493 – 4931Manganese 2Sequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30459-MONOMER.

Protein family/group databases

MEROPSiM24.A09.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized peptidase YFR006W (EC:3.4.-.-)
Gene namesi
Ordered Locus Names:YFR006W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR006W.
SGDiS000001902. YFR006W.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2417HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Uncharacterized peptidase YFR006WPRO_0000185099Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki50 – 50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP43590.

Interactioni

Protein-protein interaction databases

BioGridi31154. 12 interactions.
DIPiDIP-6829N.
IntActiP43590. 1 interaction.
MINTiMINT-631609.

Structurei

3D structure databases

ProteinModelPortaliP43590.
SMRiP43590. Positions 86-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074992.
HOGENOMiHOG000008763.
InParanoidiP43590.
KOiK14213.
OMAiLMWSVPP.
OrthoDBiEOG77M8XQ.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24_structural-domain.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCLEPISLVV FGSLVFFFGL VKYFKRGERQ RTRGILQPEY KDKYYYSKEK
60 70 80 90 100
GEEMGEVANV NEIPVKIRNH KYPAKEHNLR VKDLLLNRNP KLSKISTAFF
110 120 130 140 150
IAGEELEGNK YCDTNKDFRQ NRYFYHLSGV DIPASAILFN CSTDKLTLFL
160 170 180 190 200
PNIDEEDVIW SGMPLSLDEA MRVFDIDEAL YISDLGKKFK ELQDFAIFTT
210 220 230 240 250
DLDNVHDENI ARSLIPSDPN FFYAMDETRA IKDWYEIESI RKACQISDKS
260 270 280 290 300
HLAVMSALPI ELNELQIQAE FEYHATRQGG RSLGYDPICC SGPACGTLHY
310 320 330 340 350
VKNSEDIKGK HSILIDAGAE WRQYTSDITR CFPTSGKFTA EHREVYETVL
360 370 380 390 400
DMQNQAMERI KPGAKWDDLH ALTHKVLIKH FLSMGIFKKE FSEDEIFKRR
410 420 430 440 450
ASCAFYPHGL GHMLGLDVHD VGGNPNYDDP DPMFRYLRIR RPLKENMVIT
460 470 480 490 500
NEPGCYFNQF LIKEFLEKHP ERLEVVDMSV LKRYMYVGGV RIEDDILVTK
510 520 530
DGYENLTGIT SDPDEIEKIV QKGLKKPRSG FHVIV
Length:535
Mass (Da):61,754
Last modified:November 1, 1995 - v1
Checksum:i4D251041CE9627FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti326 – 3261S → G in AAU09720 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09245.1.
AY723803 Genomic DNA. Translation: AAU09720.1.
BK006940 Genomic DNA. Translation: DAA12446.1.
PIRiS56261.
RefSeqiNP_116661.1. NM_001179971.1.

Genome annotation databases

EnsemblFungiiBAA09245; BAA09245; BAA09245.
YFR006W; YFR006W; YFR006W.
GeneIDi850556.
KEGGisce:YFR006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09245.1.
AY723803 Genomic DNA. Translation: AAU09720.1.
BK006940 Genomic DNA. Translation: DAA12446.1.
PIRiS56261.
RefSeqiNP_116661.1. NM_001179971.1.

3D structure databases

ProteinModelPortaliP43590.
SMRiP43590. Positions 86-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31154. 12 interactions.
DIPiDIP-6829N.
IntActiP43590. 1 interaction.
MINTiMINT-631609.

Protein family/group databases

MEROPSiM24.A09.

Proteomic databases

MaxQBiP43590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09245; BAA09245; BAA09245.
YFR006W; YFR006W; YFR006W.
GeneIDi850556.
KEGGisce:YFR006W.

Organism-specific databases

EuPathDBiFungiDB:YFR006W.
SGDiS000001902. YFR006W.

Phylogenomic databases

GeneTreeiENSGT00550000074992.
HOGENOMiHOG000008763.
InParanoidiP43590.
KOiK14213.
OMAiLMWSVPP.
OrthoDBiEOG77M8XQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30459-MONOMER.

Miscellaneous databases

PROiP43590.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24_structural-domain.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
    Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-50.
  5. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYFH6_YEAST
AccessioniPrimary (citable) accession number: P43590
Secondary accession number(s): D6VTN6, Q66RB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.