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Protein

Pre-mRNA-splicing factor SAD1

Gene

SAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 107UBP-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • protein deubiquitination Source: InterPro
  • spliceosomal complex assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30458-MONOMER.

Protein family/group databases

MEROPSiC19.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SAD1
Alternative name(s):
snRNP assembly-defective protein 1
Gene namesi
Name:SAD1
Ordered Locus Names:YFR005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR005C.
SGDiS000001901. SAD1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026821 – 448Pre-mRNA-splicing factor SAD1Add BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43589.
PRIDEiP43589.

PTM databases

iPTMnetiP43589.

Interactioni

Subunit structurei

Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome.1 Publication

Protein-protein interaction databases

BioGridi31153. 14 interactors.
MINTiMINT-4485053.

Structurei

Secondary structure

1448
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 34Combined sources5
Helixi37 – 39Combined sources3
Turni49 – 51Combined sources3
Beta strandi55 – 60Combined sources6
Turni61 – 63Combined sources3
Beta strandi66 – 68Combined sources3
Helixi75 – 82Combined sources8
Beta strandi87 – 90Combined sources4
Turni91 – 93Combined sources3
Beta strandi96 – 98Combined sources3
Turni99 – 102Combined sources4
Helixi108 – 110Combined sources3
Helixi112 – 121Combined sources10
Helixi127 – 131Combined sources5
Beta strandi136 – 138Combined sources3
Beta strandi144 – 146Combined sources3
Beta strandi151 – 153Combined sources3
Helixi155 – 169Combined sources15
Helixi172 – 180Combined sources9
Helixi188 – 200Combined sources13
Beta strandi206 – 210Combined sources5
Helixi213 – 222Combined sources10
Helixi232 – 246Combined sources15
Helixi248 – 258Combined sources11
Beta strandi260 – 265Combined sources6
Beta strandi281 – 291Combined sources11
Helixi305 – 307Combined sources3
Beta strandi310 – 312Combined sources3
Helixi313 – 316Combined sources4
Helixi318 – 321Combined sources4
Beta strandi331 – 336Combined sources6
Beta strandi339 – 345Combined sources7
Beta strandi352 – 354Combined sources3
Turni355 – 358Combined sources4
Beta strandi363 – 365Combined sources3
Beta strandi368 – 372Combined sources5
Beta strandi375 – 388Combined sources14
Beta strandi403 – 412Combined sources10
Turni413 – 416Combined sources4
Beta strandi417 – 422Combined sources6
Beta strandi425 – 429Combined sources5
Helixi431 – 436Combined sources6
Beta strandi437 – 446Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MSXX-ray1.87A1-448[»]
ProteinModelPortaliP43589.
SMRiP43589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 447USPAdd BLAST298

Sequence similaritiesi

Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 107UBP-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000007992.
HOGENOMiHOG000188754.
InParanoidiP43589.
KOiK12847.
OMAiQSEPMEG.
OrthoDBiEOG092C2SDN.

Family and domain databases

CDDicd02669. Peptidase_C19M. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR033809. USP39.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PANTHERiPTHR24006:SF521. PTHR24006:SF521. 1 hit.
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVDNKRRHS EDELKQEAVK KIKSQEPNYA YLETVVREKL DFDSEKICCI
60 70 80 90 100
TLSPLNVYCC LVCGHYYQGR HEKSPAFIHS IDENHHVFLN LTSLKFYMLP
110 120 130 140 150
QNVQILHDGE VQLLNSIKFA AYPTYCPKDL EDFPRQCFDL SNRTYLNGFI
160 170 180 190 200
GFTNAATYDY AHSVLLLISH MVPVRDHFLL NHFDNQGEFI KRLSICVKKI
210 220 230 240 250
WSPKLFKHHL SVDDFVSYLK VREGLNLNPI DPRLFLLWLF NKICSSSNDL
260 270 280 290 300
KSILNHSCKG KVKIAKVENK PEASESVTGK VIVKPFWVLT LDLPEFSPFE
310 320 330 340 350
DGNSVDDLPQ INITKLLTKF TKSRSSSTST VFELTRLPQF LIFHFNRFDR
360 370 380 390 400
NSDHPVKNRN QTLVEFSSEL EILHVKYRLK ANVVHVVIKQ PSTDGNAFNG
410 420 430 440
DEKSHWITQL YDNKSEKWIE IDGINTTERE AELLFLKETF IQVWEKQE
Length:448
Mass (Da):52,167
Last modified:November 1, 1995 - v1
Checksum:iD5D7C324E60AEC01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09244.1.
AY692766 Genomic DNA. Translation: AAT92785.1.
BK006940 Genomic DNA. Translation: DAA12445.1.
PIRiS56260.
RefSeqiNP_116660.1. NM_001179970.1.

Genome annotation databases

EnsemblFungiiBAA09244; BAA09244; BAA09244.
YFR005C; YFR005C; YFR005C.
GeneIDi850555.
KEGGisce:YFR005C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09244.1.
AY692766 Genomic DNA. Translation: AAT92785.1.
BK006940 Genomic DNA. Translation: DAA12445.1.
PIRiS56260.
RefSeqiNP_116660.1. NM_001179970.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MSXX-ray1.87A1-448[»]
ProteinModelPortaliP43589.
SMRiP43589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31153. 14 interactors.
MINTiMINT-4485053.

Protein family/group databases

MEROPSiC19.972.

PTM databases

iPTMnetiP43589.

Proteomic databases

MaxQBiP43589.
PRIDEiP43589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09244; BAA09244; BAA09244.
YFR005C; YFR005C; YFR005C.
GeneIDi850555.
KEGGisce:YFR005C.

Organism-specific databases

EuPathDBiFungiDB:YFR005C.
SGDiS000001901. SAD1.

Phylogenomic databases

GeneTreeiENSGT00390000007992.
HOGENOMiHOG000188754.
InParanoidiP43589.
KOiK12847.
OMAiQSEPMEG.
OrthoDBiEOG092C2SDN.

Enzyme and pathway databases

BioCyciYEAST:G3O-30458-MONOMER.

Miscellaneous databases

PROiP43589.

Family and domain databases

CDDicd02669. Peptidase_C19M. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR033809. USP39.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PANTHERiPTHR24006:SF521. PTHR24006:SF521. 1 hit.
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAD1_YEAST
AccessioniPrimary (citable) accession number: P43589
Secondary accession number(s): D6VTN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 167 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.