Reviewed, UniProtKB/Swiss-Prot P43589 (SAD1_YEAST)

Names and origin

Protein names

Recommended name:
    Pre-mRNA-splicing factor SAD1
Alternative name(s):
    snRNP assembly-defective protein 1

Gene names

Name:	SAD1
Ordered Locus Names:	YFR005C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA. Ref.3
Subunit structure	Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome.
Subcellular location	Nucleus.
Miscellaneous	Present with 167 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Uncategorized	? Ref.3 Inferred from mutant phenotype. Source: SGD
Biological process	nuclear mRNA splicing, via spliceosome Ref.3 Inferred from direct assay. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Pre-mRNA-splicing factor SAD1		PRO_0000202682
Regions
	Zinc finger	46 – 107	62	UBP-type
Amino acid modifications
	Modified residue	10	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P43589-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D5D7C324E60AEC01	FASTA	448	52,167
	10 20 30 40 50 60 MEVDNKRRHS EDELKQEAVK KIKSQEPNYA YLETVVREKL DFDSEKICCI TLSPLNVYCC 70 80 90 100 110 120 LVCGHYYQGR HEKSPAFIHS IDENHHVFLN LTSLKFYMLP QNVQILHDGE VQLLNSIKFA 130 140 150 160 170 180 AYPTYCPKDL EDFPRQCFDL SNRTYLNGFI GFTNAATYDY AHSVLLLISH MVPVRDHFLL 190 200 210 220 230 240 NHFDNQGEFI KRLSICVKKI WSPKLFKHHL SVDDFVSYLK VREGLNLNPI DPRLFLLWLF 250 260 270 280 290 300 NKICSSSNDL KSILNHSCKG KVKIAKVENK PEASESVTGK VIVKPFWVLT LDLPEFSPFE 310 320 330 340 350 360 DGNSVDDLPQ INITKLLTKF TKSRSSSTST VFELTRLPQF LIFHFNRFDR NSDHPVKNRN 370 380 390 400 410 420 QTLVEFSSEL EILHVKYRLK ANVVHVVIKQ PSTDGNAFNG DEKSHWITQL YDNKSEKWIE 430 440 IDGINTTERE AELLFLKETF IQVWEKQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing." Lygerou Z., Christophides G., Seraphin B. Mol. Cell. Biol. 19:2008-2020(1999) [PubMed: 10022888] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract] Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09244.1. AY692766 Genomic DNA. Translation: AAT92785.1.
PIR	S56260.
RefSeq	NP_116660.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P43589. 1 interaction.
STRING	P43589.
Proteomic databases
PeptideAtlas	P43589.
Genome annotation databases
Ensembl	YFR005C; YFR005C; YFR005C; Saccharomyces cerevisiae. [Genome view]
GeneID	850555.
KEGG	sce:YFR005C.
NMPDR	fig|4932.3.peg.2291.
Organism-specific databases
CYGD	YFR005c.
SGD	S000001901. SAD1.
Phylogenomic databases
HOGENOM	P43589.
OMA	SHINVYA
OrthoDB	EOG9DNGMJ
Gene expression databases
ArrayExpress	P43589.
Genevestigator	P43589.
GermOnline	YFR005C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001394. Peptidase_C19. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00290. ZnF_UBP. 1 hit. [Graphical view]
PROSITE	PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966342.

Entry information

Entry name

SAD1_YEAST

Accession

Primary (citable) accession number: P43589

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)