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P43589

- SAD1_YEAST

UniProt

P43589 - SAD1_YEAST

Protein

Pre-mRNA-splicing factor SAD1

Gene

SAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri46 – 10762UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitinyl hydrolase activity Source: InterPro
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD
    2. spliceosomal complex assembly Source: SGD
    3. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30458-MONOMER.

    Protein family/group databases

    MEROPSiC19.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor SAD1
    Alternative name(s):
    snRNP assembly-defective protein 1
    Gene namesi
    Name:SAD1
    Ordered Locus Names:YFR005C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR005c.
    SGDiS000001901. SAD1.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 448448Pre-mRNA-splicing factor SAD1PRO_0000202682Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP43589.
    PaxDbiP43589.
    PeptideAtlasiP43589.

    Expressioni

    Gene expression databases

    GenevestigatoriP43589.

    Interactioni

    Subunit structurei

    Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome.1 Publication

    Protein-protein interaction databases

    BioGridi31153. 12 interactions.
    MINTiMINT-4485053.
    STRINGi4932.YFR005C.

    Structurei

    Secondary structure

    1
    448
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 345
    Helixi37 – 393
    Turni49 – 513
    Beta strandi55 – 606
    Turni61 – 633
    Beta strandi66 – 683
    Helixi75 – 828
    Beta strandi87 – 904
    Turni91 – 933
    Beta strandi96 – 983
    Turni99 – 1024
    Helixi108 – 1103
    Helixi112 – 12110
    Helixi127 – 1315
    Beta strandi136 – 1383
    Beta strandi144 – 1463
    Beta strandi151 – 1533
    Helixi155 – 16915
    Helixi172 – 1809
    Helixi188 – 20013
    Beta strandi206 – 2105
    Helixi213 – 22210
    Helixi232 – 24615
    Helixi248 – 25811
    Beta strandi260 – 2656
    Beta strandi281 – 29111
    Helixi305 – 3073
    Beta strandi310 – 3123
    Helixi313 – 3164
    Helixi318 – 3214
    Beta strandi331 – 3366
    Beta strandi339 – 3457
    Beta strandi352 – 3543
    Turni355 – 3584
    Beta strandi363 – 3653
    Beta strandi368 – 3725
    Beta strandi375 – 38814
    Beta strandi403 – 41210
    Turni413 – 4164
    Beta strandi417 – 4226
    Beta strandi425 – 4295
    Helixi431 – 4366
    Beta strandi437 – 44610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4MSXX-ray1.87A1-448[»]
    ProteinModelPortaliP43589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini150 – 447298USPAdd
    BLAST

    Sequence similaritiesi

    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri46 – 10762UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG259163.
    GeneTreeiENSGT00390000007992.
    HOGENOMiHOG000188754.
    KOiK12847.
    OMAiQSEPMEG.
    OrthoDBiEOG73RBMM.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    PROSITEiPS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43589-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVDNKRRHS EDELKQEAVK KIKSQEPNYA YLETVVREKL DFDSEKICCI    50
    TLSPLNVYCC LVCGHYYQGR HEKSPAFIHS IDENHHVFLN LTSLKFYMLP 100
    QNVQILHDGE VQLLNSIKFA AYPTYCPKDL EDFPRQCFDL SNRTYLNGFI 150
    GFTNAATYDY AHSVLLLISH MVPVRDHFLL NHFDNQGEFI KRLSICVKKI 200
    WSPKLFKHHL SVDDFVSYLK VREGLNLNPI DPRLFLLWLF NKICSSSNDL 250
    KSILNHSCKG KVKIAKVENK PEASESVTGK VIVKPFWVLT LDLPEFSPFE 300
    DGNSVDDLPQ INITKLLTKF TKSRSSSTST VFELTRLPQF LIFHFNRFDR 350
    NSDHPVKNRN QTLVEFSSEL EILHVKYRLK ANVVHVVIKQ PSTDGNAFNG 400
    DEKSHWITQL YDNKSEKWIE IDGINTTERE AELLFLKETF IQVWEKQE 448
    Length:448
    Mass (Da):52,167
    Last modified:November 1, 1995 - v1
    Checksum:iD5D7C324E60AEC01
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09244.1.
    AY692766 Genomic DNA. Translation: AAT92785.1.
    BK006940 Genomic DNA. Translation: DAA12445.1.
    PIRiS56260.
    RefSeqiNP_116660.1. NM_001179970.1.

    Genome annotation databases

    EnsemblFungiiYFR005C; YFR005C; YFR005C.
    GeneIDi850555.
    KEGGisce:YFR005C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09244.1 .
    AY692766 Genomic DNA. Translation: AAT92785.1 .
    BK006940 Genomic DNA. Translation: DAA12445.1 .
    PIRi S56260.
    RefSeqi NP_116660.1. NM_001179970.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4MSX X-ray 1.87 A 1-448 [» ]
    ProteinModelPortali P43589.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31153. 12 interactions.
    MINTi MINT-4485053.
    STRINGi 4932.YFR005C.

    Protein family/group databases

    MEROPSi C19.972.

    Proteomic databases

    MaxQBi P43589.
    PaxDbi P43589.
    PeptideAtlasi P43589.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR005C ; YFR005C ; YFR005C .
    GeneIDi 850555.
    KEGGi sce:YFR005C.

    Organism-specific databases

    CYGDi YFR005c.
    SGDi S000001901. SAD1.

    Phylogenomic databases

    eggNOGi NOG259163.
    GeneTreei ENSGT00390000007992.
    HOGENOMi HOG000188754.
    KOi K12847.
    OMAi QSEPMEG.
    OrthoDBi EOG73RBMM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30458-MONOMER.

    Miscellaneous databases

    NextBioi 966342.
    PROi P43589.

    Gene expression databases

    Genevestigatori P43589.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing."
      Lygerou Z., Christophides G., Seraphin B.
      Mol. Cell. Biol. 19:2008-2020(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
      Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
      Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSAD1_YEAST
    AccessioniPrimary (citable) accession number: P43589
    Secondary accession number(s): D6VTN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 167 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3