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P43589

- SAD1_YEAST

UniProt

P43589 - SAD1_YEAST

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Protein

Pre-mRNA-splicing factor SAD1

Gene

SAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri46 – 10762UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ubiquitinyl hydrolase activity Source: InterPro
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
  2. spliceosomal complex assembly Source: SGD
  3. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30458-MONOMER.

Protein family/group databases

MEROPSiC19.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SAD1
Alternative name(s):
snRNP assembly-defective protein 1
Gene namesi
Name:SAD1
Ordered Locus Names:YFR005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR005c.
SGDiS000001901. SAD1.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nucleus Source: SGD
  2. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Pre-mRNA-splicing factor SAD1PRO_0000202682Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43589.
PaxDbiP43589.
PeptideAtlasiP43589.

Expressioni

Gene expression databases

GenevestigatoriP43589.

Interactioni

Subunit structurei

Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome.1 Publication

Protein-protein interaction databases

BioGridi31153. 12 interactions.
MINTiMINT-4485053.
STRINGi4932.YFR005C.

Structurei

Secondary structure

1
448
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 345Combined sources
Helixi37 – 393Combined sources
Turni49 – 513Combined sources
Beta strandi55 – 606Combined sources
Turni61 – 633Combined sources
Beta strandi66 – 683Combined sources
Helixi75 – 828Combined sources
Beta strandi87 – 904Combined sources
Turni91 – 933Combined sources
Beta strandi96 – 983Combined sources
Turni99 – 1024Combined sources
Helixi108 – 1103Combined sources
Helixi112 – 12110Combined sources
Helixi127 – 1315Combined sources
Beta strandi136 – 1383Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi151 – 1533Combined sources
Helixi155 – 16915Combined sources
Helixi172 – 1809Combined sources
Helixi188 – 20013Combined sources
Beta strandi206 – 2105Combined sources
Helixi213 – 22210Combined sources
Helixi232 – 24615Combined sources
Helixi248 – 25811Combined sources
Beta strandi260 – 2656Combined sources
Beta strandi281 – 29111Combined sources
Helixi305 – 3073Combined sources
Beta strandi310 – 3123Combined sources
Helixi313 – 3164Combined sources
Helixi318 – 3214Combined sources
Beta strandi331 – 3366Combined sources
Beta strandi339 – 3457Combined sources
Beta strandi352 – 3543Combined sources
Turni355 – 3584Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi368 – 3725Combined sources
Beta strandi375 – 38814Combined sources
Beta strandi403 – 41210Combined sources
Turni413 – 4164Combined sources
Beta strandi417 – 4226Combined sources
Beta strandi425 – 4295Combined sources
Helixi431 – 4366Combined sources
Beta strandi437 – 44610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MSXX-ray1.87A1-448[»]
ProteinModelPortaliP43589.
SMRiP43589. Positions 29-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini150 – 447298USPAdd
BLAST

Sequence similaritiesi

Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri46 – 10762UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG259163.
GeneTreeiENSGT00390000007992.
HOGENOMiHOG000188754.
InParanoidiP43589.
KOiK12847.
OMAiQSEPMEG.
OrthoDBiEOG73RBMM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43589-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVDNKRRHS EDELKQEAVK KIKSQEPNYA YLETVVREKL DFDSEKICCI
60 70 80 90 100
TLSPLNVYCC LVCGHYYQGR HEKSPAFIHS IDENHHVFLN LTSLKFYMLP
110 120 130 140 150
QNVQILHDGE VQLLNSIKFA AYPTYCPKDL EDFPRQCFDL SNRTYLNGFI
160 170 180 190 200
GFTNAATYDY AHSVLLLISH MVPVRDHFLL NHFDNQGEFI KRLSICVKKI
210 220 230 240 250
WSPKLFKHHL SVDDFVSYLK VREGLNLNPI DPRLFLLWLF NKICSSSNDL
260 270 280 290 300
KSILNHSCKG KVKIAKVENK PEASESVTGK VIVKPFWVLT LDLPEFSPFE
310 320 330 340 350
DGNSVDDLPQ INITKLLTKF TKSRSSSTST VFELTRLPQF LIFHFNRFDR
360 370 380 390 400
NSDHPVKNRN QTLVEFSSEL EILHVKYRLK ANVVHVVIKQ PSTDGNAFNG
410 420 430 440
DEKSHWITQL YDNKSEKWIE IDGINTTERE AELLFLKETF IQVWEKQE
Length:448
Mass (Da):52,167
Last modified:November 1, 1995 - v1
Checksum:iD5D7C324E60AEC01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09244.1.
AY692766 Genomic DNA. Translation: AAT92785.1.
BK006940 Genomic DNA. Translation: DAA12445.1.
PIRiS56260.
RefSeqiNP_116660.1. NM_001179970.1.

Genome annotation databases

EnsemblFungiiYFR005C; YFR005C; YFR005C.
GeneIDi850555.
KEGGisce:YFR005C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09244.1 .
AY692766 Genomic DNA. Translation: AAT92785.1 .
BK006940 Genomic DNA. Translation: DAA12445.1 .
PIRi S56260.
RefSeqi NP_116660.1. NM_001179970.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MSX X-ray 1.87 A 1-448 [» ]
ProteinModelPortali P43589.
SMRi P43589. Positions 29-448.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31153. 12 interactions.
MINTi MINT-4485053.
STRINGi 4932.YFR005C.

Protein family/group databases

MEROPSi C19.972.

Proteomic databases

MaxQBi P43589.
PaxDbi P43589.
PeptideAtlasi P43589.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR005C ; YFR005C ; YFR005C .
GeneIDi 850555.
KEGGi sce:YFR005C.

Organism-specific databases

CYGDi YFR005c.
SGDi S000001901. SAD1.

Phylogenomic databases

eggNOGi NOG259163.
GeneTreei ENSGT00390000007992.
HOGENOMi HOG000188754.
InParanoidi P43589.
KOi K12847.
OMAi QSEPMEG.
OrthoDBi EOG73RBMM.

Enzyme and pathway databases

BioCyci YEAST:G3O-30458-MONOMER.

Miscellaneous databases

NextBioi 966342.
PROi P43589.

Gene expression databases

Genevestigatori P43589.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
PROSITEi PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing."
    Lygerou Z., Christophides G., Seraphin B.
    Mol. Cell. Biol. 19:2008-2020(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
    Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
    Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSAD1_YEAST
AccessioniPrimary (citable) accession number: P43589
Secondary accession number(s): D6VTN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 167 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3