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P43589 (SAD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor SAD1
Alternative name(s):
snRNP assembly-defective protein 1
Gene names
Name:SAD1
Ordered Locus Names:YFR005C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA. Ref.4

Subunit structure

Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome. Ref.5

Subcellular location

Nucleus Ref.4 Ref.6.

Miscellaneous

Present with 167 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionRibonucleoprotein
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA splicing, via spliceosome

Inferred from direct assay Ref.4. Source: SGD

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from direct assay Ref.4. Source: SGD

spliceosomal complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionzinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448Pre-mRNA-splicing factor SAD1
PRO_0000202682

Regions

Domain150 – 447298USP
Zinc finger46 – 10762UBP-type

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P43589 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D5D7C324E60AEC01

FASTA44852,167
        10         20         30         40         50         60 
MEVDNKRRHS EDELKQEAVK KIKSQEPNYA YLETVVREKL DFDSEKICCI TLSPLNVYCC 

        70         80         90        100        110        120 
LVCGHYYQGR HEKSPAFIHS IDENHHVFLN LTSLKFYMLP QNVQILHDGE VQLLNSIKFA 

       130        140        150        160        170        180 
AYPTYCPKDL EDFPRQCFDL SNRTYLNGFI GFTNAATYDY AHSVLLLISH MVPVRDHFLL 

       190        200        210        220        230        240 
NHFDNQGEFI KRLSICVKKI WSPKLFKHHL SVDDFVSYLK VREGLNLNPI DPRLFLLWLF 

       250        260        270        280        290        300 
NKICSSSNDL KSILNHSCKG KVKIAKVENK PEASESVTGK VIVKPFWVLT LDLPEFSPFE 

       310        320        330        340        350        360 
DGNSVDDLPQ INITKLLTKF TKSRSSSTST VFELTRLPQF LIFHFNRFDR NSDHPVKNRN 

       370        380        390        400        410        420 
QTLVEFSSEL EILHVKYRLK ANVVHVVIKQ PSTDGNAFNG DEKSHWITQL YDNKSEKWIE 

       430        440 
IDGINTTERE AELLFLKETF IQVWEKQE 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing."
Lygerou Z., Christophides G., Seraphin B.
Mol. Cell. Biol. 19:2008-2020(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Composition and functional characterization of the yeast spliceosomal penta-snRNP."
Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50617 Genomic DNA. Translation: BAA09244.1.
AY692766 Genomic DNA. Translation: AAT92785.1.
BK006940 Genomic DNA. Translation: DAA12445.1.
PIRS56260.
RefSeqNP_116660.1. NM_001179970.1.

3D structure databases

ProteinModelPortalP43589.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31153. 12 interactions.
MINTMINT-4485053.
STRING4932.YFR005C.

Protein family/group databases

MEROPSC19.972.

Proteomic databases

PaxDbP43589.
PeptideAtlasP43589.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR005C; YFR005C; YFR005C.
GeneID850555.
KEGGsce:YFR005C.

Organism-specific databases

CYGDYFR005c.
SGDS000001901. SAD1.

Phylogenomic databases

eggNOGNOG259163.
GeneTreeENSGT00390000007992.
HOGENOMHOG000188754.
KOK12847.
OMAPPYLLFH.
OrthoDBEOG73RBMM.

Enzyme and pathway databases

BioCycYEAST:G3O-30458-MONOMER.

Gene expression databases

GenevestigatorP43589.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEPS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966342.
PROP43589.

Entry information

Entry nameSAD1_YEAST
AccessionPrimary (citable) accession number: P43589
Secondary accession number(s): D6VTN5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families