P43587 (YPI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type 1 phosphatases regulator YPI1

Gene names

Name:	YPI1
Ordered Locus Names:	YFR003C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control. Regulates the nuclear localization of type 1 phosphatase GLC7 and SDS22, and is involved in the regulation of mRNA 3'-end processing. May also regulate the activity of type 1 phosphatase PPZ1. Ref.4 Ref.6 Ref.8 Ref.12
Subunit structure	Interacts with GLC7, PPZ1 and SDS22. Ref.4 Ref.8 Ref.12
Subcellular location	Nucleus Ref.8 Ref.12.
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the YPI1 family.
Caution	Was originally (Ref.4) thought to be an inhibitor of type 1 phosphatases using in vitro experiments, but further in vivo experiments (Ref.12) showed that it was rather an activator of these phosphatases.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from mutant phenotype Ref.4. Source: SGD protein dephosphorylation Inferred from direct assay Ref.4. Source: SGD
Cellular component	nucleus Inferred from direct assay Ref.8. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase inhibitor activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GLC7	P32598	6	EBI-22913,EBI-13715
SDS22	P36047	4	EBI-22913,EBI-16783

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	Type 1 phosphatases regulator YPI1		PRO_0000202681
Regions
	Motif	51 – 53	3	GLC7-binding
	Compositional bias	93 – 103	11	Poly-Ser
Amino acid modifications
	Modified residue	20	1	Phosphothreonine Ref.13
	Modified residue	22	1	Phosphoserine Ref.7 Ref.13
	Modified residue	131	1	Phosphoserine Ref.10 Ref.11 Ref.13
	Modified residue	132	1	Phosphotyrosine Ref.13
	Modified residue	133	1	Phosphoserine Ref.7 Ref.9 Ref.10 Ref.11 Ref.13
	Modified residue	137	1	Phosphotyrosine Ref.13
Experimental info
	Mutagenesis	51	1	V → A: Prevents interaction with GLC7. Ref.12
	Mutagenesis	53	1	W → A: Prevents interaction with GLC7 and reduces nuclear levels of GLC7 and SDS22. Ref.4 Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P43587 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E191184DCD5EF8A1	FASTA	155	18,187
	10 20 30 40 50 60 MSGNQMAMGS EQQQTVGSRT VSVEEVPAVL QLRATQDPPR SQEAMPTRHN VRWEENVIDN 70 80 90 100 110 120 ENMNKKKTKI CCIFHPQNED EEECNHHSDD DGSSSSGSSS SESENEKDLD FNERRQRRLE 130 140 150 RRHRKLEKKR SYSPNAYEIQ PDYSEYRRKQ QEKKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Molecular characterization of Ypi1, a novel Saccharomyces cerevisiae type 1 protein phosphatase inhibitor." Garcia-Gimeno M.A., Munoz I., Arino J., Sanz P. J. Biol. Chem. 278:47744-47752(2003) [PubMed: 14506263] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TRP-53, INTERACTION WITH GLC7 AND PPZ1.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Regulation of yeast mRNA 3' end processing by phosphorylation." He X., Moore C. Mol. Cell 19:619-629(2005) [PubMed: 16137619] [Abstract] Cited for: FUNCTION.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-133, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"YPI1 and SDS22 proteins regulate the nuclear localization and function of yeast type 1 phosphatase Glc7." Pedelini L., Marquina M., Arino J., Casamayor A., Sanz L., Bollen M., Sanz P., Garcia-Gimeno M.A. J. Biol. Chem. 282:3282-3292(2007) [PubMed: 17142459] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLC7 AND SDS22.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-133, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-133, MASS SPECTROMETRY.
[12]	"Ypi1, a positive regulator of nuclear protein phosphatase type 1 activity in Saccharomyces cerevisiae." Bharucha J.P., Larson J.R., Gao L., Daves L.K., Tatchell K. Mol. Biol. Cell 19:1032-1045(2008) [PubMed: 18172024] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLC7, MUTAGENESIS OF VAL-51 AND TRP-53.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-22; SER-131; TYR-132; SER-133 AND TYR-137, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09242.1. AY557803 Genomic DNA. Translation: AAS56129.1. BK006940 Genomic DNA. Translation: DAA12443.1.
PIR	S56258.
RefSeq	NP_116658.1. NM_001179968.1.
3D structure databases
ProteinModelPortal	P43587.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5442N.
IntAct	P43587. 14 interactions.
MINT	MINT-495082.
STRING	P43587.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR003C; YFR003C; YFR003C.
GeneID	850553.
KEGG	sce:YFR003C.
NMPDR	fig|4932.3.peg.2289.
Organism-specific databases
CYGD	YFR003c.
SGD	S000001899. YPI1.
Phylogenomic databases
eggNOG	fuNOG12731.
GeneTree	EFGT00050000006627.
HOGENOM	HBG395575.
OMA	VIDNENM.
OrthoDB	EOG49632N.
Gene expression databases
ArrayExpress	P43587.
Genevestigator	P43587.
GermOnline	YFR003C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011107. PPI_Ypi1. [Graphical view]
PANTHER	PTHR20835. PPI_Ypi1. 1 hit.
Pfam	PF07491. PPI_Ypi1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966336.

Entry information

Entry name

YPI1_YEAST

Accession

Primary (citable) accession number: P43587
Secondary accession number(s): D6VTN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program