60S ribosomal subunit assembly/export protein LOC1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P43586 (LOC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal subunit assembly/export protein LOC1

Alternative name(s):
Localization of ASH1 mRNA protein 1

Gene names

Name:	LOC1
Ordered Locus Names:	YFR001W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for efficient assembly and nuclear export of the 60S ribosomal subunit. Involved in asymmetric localization of ASH1 mRNA. Ref.5 Ref.11
Subunit structure	Component of the 66S pre-ribosomal particle. Interacts with NOP7, RRP1, RRP15 and SHE2. Ref.6 Ref.9 Ref.10 Ref.12
Subcellular location	Nucleus › nucleolus Ref.5 Ref.7.
Miscellaneous	Present with 3650 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the LOC1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transport mRNA transport
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD intracellular mRNA localization Inferred from mutant phenotype Ref.5. Source: SGD mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW ribosomal large subunit biogenesis Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	nucleolus Inferred from direct assay Ref.11. Source: SGD preribosome, large subunit precursor Inferred from direct assay Ref.6. Source: SGD
Molecular function	mRNA binding Inferred from direct assay Ref.5. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRX1	Q08235	3	EBI-22906,EBI-3775
NOC2	P39744	3	EBI-22906,EBI-29259

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

60S ribosomal subunit assembly/export protein LOC1

PRO_0000202680

Regions

Coiled coil

120 – 166

Potential

Compositional bias

39 – 199

161

Lys-rich

Sequences

Sequence

Length

Mass (Da)

Tools

P43586 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 69970294E9C980F9
Show »

FASTA

204

23,590

        10         20         30         40         50         60 
MAPKKPSKRQ NLRREVAPEV FQDSQARNQL ANVPHLTEKS AQRKPSKTKV KKEQSLARLY 

        70         80         90        100        110        120 
GAKKDKKGKY SEKDLNIPTL NRAIVPGVKI RRGKKGKKFI ADNDTLTLNR LITTIGDKYD 

       130        140        150        160        170        180 
DIAESKLEKA RRLEEIRELK RKEIERKEAL KQDKLEEKKD EIKKKSSVAR TIRRKNKRDM 

       190        200 
LKSEAKASES KTEGRKVKKV SFAQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI." Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y. Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast." Long R.M., Gu W., Meng X., Gonsalvez G.B., Singer R.H., Chartrand P. J. Cell Biol. 153:307-318(2001) [PubMed: 11309412] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Composition and functional characterization of yeast 66S ribosome assembly intermediates." Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr. Mol. Cell 8:505-515(2001) [PubMed: 11583614] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH NOP7.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation." Horsey E.W., Jakovljevic J., Miles T.D., Harnpicharnchai P., Woolford J.L. Jr. RNA 10:813-827(2004) [PubMed: 15100437] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH RRP1.
[10]	"Rrp15p, a novel component of pre-ribosomal particles required for 60S ribosome subunit maturation." De Marchis M.L., Giorgi A., Schinina M.E., Bozzoni I., Fatica A. RNA 11:495-502(2005) [PubMed: 15769876] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH RRP15.
[11]	"Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit." Urbinati C.R., Gonsalvez G.B., Aris J.P., Long R.M. Mol. Genet. Genomics 276:369-377(2006) [PubMed: 16871394] [Abstract] Cited for: FUNCTION.
[12]	"Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p." Shen Z., Paquin N., Forget A., Chartrand P. Mol. Biol. Cell 20:2265-2275(2009) [PubMed: 19244342] [Abstract] Cited for: INTERACTION WITH SHE2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09240.1. AY557809 Genomic DNA. Translation: AAS56135.1. BK006940 Genomic DNA. Translation: DAA12441.1.
PIR	S56256.
RefSeq	NP_116656.1. NM_001179966.1.
3D structure databases
ProteinModelPortal	P43586.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6615N.
IntAct	P43586. 53 interactions.
MINT	MINT-620051.
STRING	P43586.
Proteomic databases
PeptideAtlas	P43586.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR001W; YFR001W; YFR001W.
GeneID	850551.
KEGG	sce:YFR001W.
NMPDR	fig\|4932.3.peg.2287.
Organism-specific databases
CYGD	YFR001w.
SGD	S000001897. LOC1.
Phylogenomic databases
eggNOG	fuNOG09797.
GeneTree	EFGT00050000005814.
HOGENOM	HBG396584.
OMA	SINDKYD.
OrthoDB	EOG4BK8DH.
Gene expression databases
ArrayExpress	P43586.
Genevestigator	P43586.
GermOnline	YFR001W. Saccharomyces cerevisiae.
Family and domain databases
KO	K14817.
ProtoNet	Search...
Other
NextBio	966330.

Entry information

Entry name

LOC1_YEAST

Accession

Primary (citable) accession number: P43586
Secondary accession number(s): D6VTN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents