60S ribosomal subunit assembly/export protein LOC1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P43586 (LOC1_YEAST)

Last modified January 19, 2010. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
60S ribosomal subunit assembly/export protein LOC1
Alternative name(s):
Localization of ASH1 mRNA protein 1

Gene names

Name:	LOC1
Ordered Locus Names:	YFR001W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for efficient assembly and nuclear export of the 60S ribosomal subunit. Involved in asymmetric localization of ASH1 mRNA. Ref.4 Ref.10
Subunit structure	Component of the 66S pre-ribosomal particle. Interacts with NOP7, RRP1 and RRP15. Ref.5 Ref.8 Ref.9
Subcellular location	Nucleus › nucleolus Ref.4 Ref.6.
Miscellaneous	Present with 3650 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the LOC1 family.

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Ontologies

Keywords
Biological process	Ribosome biogenesis Transport mRNA transport
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.10 Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.10 Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.10 Inferred from mutant phenotype. Source: SGD intracellular mRNA localization Ref.4 Inferred from mutant phenotype. Source: SGD mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW ribosomal large subunit biogenesis Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	nucleolus Ref.10 Inferred from direct assay. Source: SGD preribosome, large subunit precursor Ref.5 Inferred from direct assay. Source: SGD
Molecular function	mRNA binding Ref.4 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
BRX1	Q08235	1	EBI-22906,EBI-3775
CGR1	P53188	1	EBI-22906,EBI-23731
EBP2	P36049	1	EBI-22906,EBI-6289
FPR4	Q06205	1	EBI-22906,EBI-6956
ISU1	Q03020	1	EBI-22906,EBI-29901
NOC2	P39744	1	EBI-22906,EBI-29259
PWP1	P21304	1	EBI-22906,EBI-14328
UBP10	P53874	1	EBI-22906,EBI-19873

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

60S ribosomal subunit assembly/export protein LOC1

PRO_0000202680

Regions

Coiled coil

120 – 166

Potential

Compositional bias

39 – 199

161

Lys-rich

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Sequences

Sequence

Length

Mass (Da)

Tools

P43586-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 69970294E9C980F9
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FASTA

204

23,590

        10         20         30         40         50         60 
MAPKKPSKRQ NLRREVAPEV FQDSQARNQL ANVPHLTEKS AQRKPSKTKV KKEQSLARLY 

        70         80         90        100        110        120 
GAKKDKKGKY SEKDLNIPTL NRAIVPGVKI RRGKKGKKFI ADNDTLTLNR LITTIGDKYD 

       130        140        150        160        170        180 
DIAESKLEKA RRLEEIRELK RKEIERKEAL KQDKLEEKKD EIKKKSSVAR TIRRKNKRDM 

       190        200 
LKSEAKASES KTEGRKVKKV SFAQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI." Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y. Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast." Long R.M., Gu W., Meng X., Gonsalvez G.B., Singer R.H., Chartrand P. J. Cell Biol. 153:307-318(2001) [PubMed: 11309412] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Composition and functional characterization of yeast 66S ribosome assembly intermediates." Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr. Mol. Cell 8:505-515(2001) [PubMed: 11583614] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH NOP7.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation." Horsey E.W., Jakovljevic J., Miles T.D., Harnpicharnchai P., Woolford J.L. Jr. RNA 10:813-827(2004) [PubMed: 15100437] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH RRP1.
[9]	"Rrp15p, a novel component of pre-ribosomal particles required for 60S ribosome subunit maturation." De Marchis M.L., Giorgi A., Schinina M.E., Bozzoni I., Fatica A. RNA 11:495-502(2005) [PubMed: 15769876] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH RRP15.
[10]	"Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit." Urbinati C.R., Gonsalvez G.B., Aris J.P., Long R.M. Mol. Genet. Genomics 276:369-377(2006) [PubMed: 16871394] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09240.1. AY557809 Genomic DNA. Translation: AAS56135.1.
PIR	S56256.
RefSeq	NP_116656.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6615N.
IntAct	P43586. 56 interactions.
STRING	P43586.
Proteomic databases
PeptideAtlas	P43586.
PRIDE	P43586.
Genome annotation databases
Ensembl	YFR001W; YFR001W; YFR001W; Saccharomyces cerevisiae. [Genome view]
GeneID	850551.
KEGG	sce:YFR001W.
NMPDR	fig\|4932.3.peg.2287.
Organism-specific databases
CYGD	YFR001w.
SGD	S000001897. LOC1.
Phylogenomic databases
eggNOG	fuNOG09797.
HOGENOM	HBG396584.
OMA	VKSINDK.
OrthoDB	EOG9ZPGBK.
PhylomeDB	P43586.
Gene expression databases
ArrayExpress	P43586.
Genevestigator	P43586.
GermOnline	YFR001W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	966330.

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Entry information

Entry name

LOC1_YEAST

Accession

Primary (citable) accession number: P43586

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents