P43579 (IES1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ino eighty subunit 1

Gene names

Name:	IES1
Ordered Locus Names:	YFL013C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin-remodeling complex.
Subunit structure	Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80.
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 1400 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	nucleosome mobilization Inferred from direct assay Ref.4. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Ino80 complex Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 692	692	Ino eighty subunit 1		PRO_0000084153
Regions
	Coiled coil	340 – 385	46	Potential
Amino acid modifications
	Modified residue	27	1	Phosphoserine Ref.7
	Modified residue	34	1	Phosphoserine Ref.8
	Modified residue	36	1	Phosphoserine Ref.8
	Modified residue	47	1	Phosphoserine Ref.8
	Modified residue	443	1	Phosphoserine Ref.8
	Modified residue	446	1	Phosphothreonine Ref.8
	Modified residue	493	1	Phosphoserine Ref.7
	Modified residue	504	1	Phosphoserine Ref.7
	Modified residue	507	1	Phosphothreonine Ref.7
	Modified residue	516	1	Phosphothreonine Ref.8
	Modified residue	567	1	Phosphothreonine Ref.8
	Modified residue	574	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	219	1	D → G in AAU09719. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P43579 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9C0084A36431B241	FASTA	692	78,753
	10 20 30 40 50 60 MGKRVYDPIH DTFQLREDNS DETKADSPMQ SVKSGSQEEA SPSSIQSETE TVTTKSIPVI 70 80 90 100 110 120 HEIEIDDKND DDSTQSEEEN TNILLNFEPS TVPEATGAST ATGPVTTNTV RRKPKESNAS 130 140 150 160 170 180 KYNRHLKKPD GEPFNRKDIQ FSFMQELLMD KRQIFTNVLK PLYKNSIVPI NIDGDKLSIN 190 200 210 220 230 240 VTDKEYDART FVFNDKLTFA QLYVLTIATS IKCSKILRDK LLLDQQVAFS TCVLALLVNI 250 260 270 280 290 300 GRLNTTINFY LEMTSQLRTF HSVPVLQLHA NDPKLLQDTP RLKSILKNLP WGNEQLSLME 310 320 330 340 350 360 TYKKVDQNDG EVDTVNKFNI INMLFSICDN SGLIDKRFLS KYVEVESKAQ EQDMVDEQNE 370 380 390 400 410 420 VKETEAENEK QESKAAYATT LFDILDYSKY EPKDRSNILI WLLYIHLETN LSQEEVEESV 430 440 450 460 470 480 RFFNGLEDGA PAGKFILRCT ERSYDTDPED ELEFGANQRI KRREFMSKME EGRKRERTNV 490 500 510 520 530 540 TEVKKPSIGG DKSEEDGEGE DDKSEETVEE TRSLLTPTPI LESSSPMTLN RKKVTPQLPK 550 560 570 580 590 600 VTPAAPTETE EEITSAAIID KNDLNLTPLK KYNSSATVNK VDKLISLDLN KHVSENGKTQ 610 620 630 640 650 660 EEFLADLKKS QVPNRLKRRD IGLIKIFNEF EDIPVASVLG IRGKKRKKFK DNLLGFETDF 670 680 690 MKNLGASKKV LLNKIERAEI DDEEATAMFK LE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Involvement of actin-related proteins in ATP-dependent chromatin remodeling." Shen X., Ranallo R., Choi E., Wu C. Mol. Cell 12:147-155(2003) [PubMed: 12887900] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION IN THE INO80 COMPLEX.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27; SER-493; SER-504 AND THR-507, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-36; SER-47; SER-443; THR-446; THR-516; THR-567 AND SER-574, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09225.1. Z46255 Genomic DNA. Translation: CAA86347.1. AY723802 Genomic DNA. Translation: AAU09719.1. BK006940 Genomic DNA. Translation: DAA12426.1.
PIR	S48316.
RefSeq	NP_116641.1. NM_001179953.1.
3D structure databases
ProteinModelPortal	P43579.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3869N.
IntAct	P43579. 18 interactions.
MINT	MINT-534170.
STRING	P43579.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL013C; YFL013C; YFL013C.
GeneID	850534.
KEGG	sce:YFL013C.
NMPDR	fig|4932.3.peg.2271.
Organism-specific databases
CYGD	YFL013c.
SGD	S000001881. IES1.
Phylogenomic databases
eggNOG	fuNOG07177.
HOGENOM	HBG203595.
OMA	MTSQLRT.
OrthoDB	EOG4WM83Q.
Gene expression databases
ArrayExpress	P43579.
Genevestigator	P43579.
GermOnline	YFL013C. Saccharomyces cerevisiae.
Family and domain databases
KO	K11675.
ProtoNet	Search...
Other
NextBio	966283.

Entry information

Entry name

IES1_YEAST

Accession

Primary (citable) accession number: P43579
Secondary accession number(s): D6VTL6, E9P952

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program