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Protein

Glucosamine 6-phosphate N-acetyltransferase

Gene

GNA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 2550 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + D-glucosamine 6-phosphate = CoA + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glucosamine 6-phosphate N-acetyltransferase (GNA1)
  2. Phosphoacetylglucosamine mutase (PCM1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28Substrate1 Publication1
Binding sitei134Substrate; via carbonyl oxygen1 Publication1
Binding sitei158Substrate1 Publication1

GO - Molecular functioni

  • glucosamine 6-phosphate N-acetyltransferase activity Source: SGD

GO - Biological processi

  • UDP-N-acetylglucosamine biosynthetic process Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YFL017C-MONOMER
YEAST:YFL017C-MONOMER
ReactomeiR-SCE-446210 Synthesis of UDP-N-acetyl-glucosamine
SABIO-RKiP43577
UniPathwayiUPA00113; UER00529

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine 6-phosphate N-acetyltransferase (EC:2.3.1.41 Publication)
Alternative name(s):
Phosphoglucosamine acetylase
Phosphoglucosamine transacetylase
Gene namesi
Name:GNA1
Synonyms:PAT1
Ordered Locus Names:YFL017C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL017C
SGDiS000001877 GNA1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000745522 – 159Glucosamine 6-phosphate N-acetyltransferaseAdd BLAST158

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43577
PaxDbiP43577
PRIDEiP43577

PTM databases

iPTMnetiP43577

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31130, 309 interactors
DIPiDIP-1349N
IntActiP43577, 7 interactors
MINTiP43577
STRINGi4932.YFL017C

Structurei

Secondary structure

1159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Beta strandi7 – 11Combined sources5
Helixi14 – 16Combined sources3
Helixi17 – 24Combined sources8
Turni25 – 27Combined sources3
Helixi35 – 47Combined sources13
Beta strandi60 – 62Combined sources3
Beta strandi65 – 69Combined sources5
Turni70 – 73Combined sources4
Beta strandi74 – 85Combined sources12
Helixi88 – 91Combined sources4
Beta strandi93 – 102Combined sources10
Helixi104 – 106Combined sources3
Helixi111 – 125Combined sources15
Beta strandi129 – 135Combined sources7
Helixi137 – 139Combined sources3
Helixi140 – 145Combined sources6
Beta strandi149 – 158Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I12X-ray1.30A/B/C/D1-159[»]
1I1DX-ray1.80A/B/C/D1-159[»]
1I21X-ray2.40A/B/M/N/X/Y1-159[»]
ProteinModelPortaliP43577
SMRiP43577
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43577

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 159N-acetyltransferasePROSITE-ProRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 89Substrate binding1 Publication4
Regioni98 – 100Substrate binding1 Publication3
Regioni100 – 102Acetyl-CoA bindingCombined sources1 Publication3
Regioni108 – 113Acetyl-CoA bindingCombined sources1 Publication6
Regioni129 – 130Substrate binding1 Publication2
Regioni143 – 145Acetyl-CoA bindingCombined sources1 Publication3

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008666
HOGENOMiHOG000106325
InParanoidiP43577
KOiK00621
OMAiVGCYKSI
OrthoDBiEOG092C55DH

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPDGFYIR RMEEGDLEQV TETLKVLTTV GTITPESFSK LIKYWNEATV
60 70 80 90 100
WNDNEDKKIM QYNPMVIVDK RTETVAATGN IIIERKIIHE LGLCGHIEDI
110 120 130 140 150
AVNSKYQGQG LGKLLIDQLV TIGFDYGCYK IILDCDEKNV KFYEKCGFSN

AGVEMQIRK
Length:159
Mass (Da):18,135
Last modified:November 1, 1995 - v1
Checksum:i2D2DD2D43A74C6F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112 – 159GKLLI…MQIRK → ASS in CAA86352 (Ref. 4) CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017626 Genomic DNA Translation: BAA36495.1
D50617 Genomic DNA Translation: BAA09221.1
Z46255 Genomic DNA Translation: CAA86352.1
AY558564 Genomic DNA Translation: AAS56890.1
BK006940 Genomic DNA Translation: DAA12423.1
PIRiS56237
RefSeqiNP_116637.1, NM_001179949.1

Genome annotation databases

EnsemblFungiiBAA09221; BAA09221; BAA09221
YFL017C; YFL017C; YFL017C
GeneIDi850529
KEGGisce:YFL017C

Similar proteinsi

Entry informationi

Entry nameiGNA1_YEAST
AccessioniPrimary (citable) accession number: P43577
Secondary accession number(s): D6VTL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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