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P43574

- GAT1_YEAST

UniProt

P43574 - GAT1_YEAST

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Protein
Transcriptional regulatory protein GAT1
Gene
GAT1, YFL021W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Positive regulator of multiple nitrogen catabolic genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri310 – 33425GATA-type
Add
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  2. RNA polymerase II transcription factor binding transcription factor activity Source: SGD
  3. sequence-specific DNA binding Source: SGD
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. nitrogen catabolite activation of transcription Source: SGD
  2. nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: GOC
  4. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30439-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein GAT1
Gene namesi
Name:GAT1
Ordered Locus Names:YFL021W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL021w.
SGDiS000001873. GAT1.

Subcellular locationi

Nucleus Reviewed prediction

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Transcriptional regulatory protein GAT1
PRO_0000083484Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671Phosphoserine1 Publication
Modified residuei262 – 2621Phosphoserine2 Publications
Modified residuei270 – 2701Phosphoserine2 Publications
Modified residuei369 – 3691Phosphothreonine1 Publication
Modified residuei399 – 3991Phosphoserine2 Publications
Modified residuei418 – 4181Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43574.
PaxDbiP43574.

Expressioni

Gene expression databases

GenevestigatoriP43574.

Interactioni

Protein-protein interaction databases

BioGridi31125. 34 interactions.
DIPiDIP-4276N.
IntActiP43574. 7 interactions.
MINTiMINT-525395.
STRINGi4932.YFL021W.

Structurei

3D structure databases

ProteinModelPortaliP43574.
SMRiP43574. Positions 310-349.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi151 – 1588Poly-Asp

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
GeneTreeiENSGT00750000117454.
KOiK09184.
OrthoDBiEOG7TQVCN.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR013860. DUF1752_fun.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF08550. DUF1752. 1 hit.
PF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43574-1 [UniParc]FASTAAdd to Basket

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MHVFFPLLFR PSPVLFIACA YIYIDIYIHC TRCTVVNITM STNRVPNLDP    50
DLNLNKEIWD LYSSAQKILP DSNRILNLSW RLHNRTSFHR INRIMQHSNS 100
IMDFSASPFA SGVNAAGPGN NDLDDTDTDN QQFFLSDMNL NGSSVFENVF 150
DDDDDDDDVE THSIVHSDLL NDMDSASQRA SHNASGFPNF LDTSCSSSFD 200
DHFIFTNNLP FLNNNSINNN HSHNSSHNNN SPSIANNTNA NTNTNTSAST 250
NTNSPLLRRN PSPSIVKPGS RRNSSVRKKK PALKKIKSST SVQSSATPPS 300
NTSSNPDIKC SNCTTSTTPL WRKDPKGLPL CNACGLFLKL HGVTRPLSLK 350
TDIIKKRQRS STKINNNITP PPSSSLNPGA AGKKKNYTAS VAASKRKNSL 400
NIVAPLKSQD IPIPKIASPS IPQYLRSNTR HHLSSSVPIE AETFSSFRPD 450
MNMTMNMNLH NASTSSFNNE AFWKPLDSAI DHHSGDTNPN SNMNTTPNGN 500
LSLDWLNLNL 510
Length:510
Mass (Da):56,328
Last modified:November 1, 1995 - v1
Checksum:i62D805E42695F35F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U27344 Genomic DNA. Translation: AAB03516.1.
D50617 Genomic DNA. Translation: BAA09217.1.
BK006940 Genomic DNA. Translation: DAA12419.1.
PIRiS56233.
RefSeqiNP_116632.1. NM_001179945.1.

Genome annotation databases

EnsemblFungiiYFL021W; YFL021W; YFL021W.
GeneIDi850523.
KEGGisce:YFL021W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U27344 Genomic DNA. Translation: AAB03516.1 .
D50617 Genomic DNA. Translation: BAA09217.1 .
BK006940 Genomic DNA. Translation: DAA12419.1 .
PIRi S56233.
RefSeqi NP_116632.1. NM_001179945.1.

3D structure databases

ProteinModelPortali P43574.
SMRi P43574. Positions 310-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31125. 34 interactions.
DIPi DIP-4276N.
IntActi P43574. 7 interactions.
MINTi MINT-525395.
STRINGi 4932.YFL021W.

Proteomic databases

MaxQBi P43574.
PaxDbi P43574.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL021W ; YFL021W ; YFL021W .
GeneIDi 850523.
KEGGi sce:YFL021W.

Organism-specific databases

CYGDi YFL021w.
SGDi S000001873. GAT1.

Phylogenomic databases

eggNOGi COG5641.
GeneTreei ENSGT00750000117454.
KOi K09184.
OrthoDBi EOG7TQVCN.

Enzyme and pathway databases

BioCyci YEAST:G3O-30439-MONOMER.

Miscellaneous databases

NextBioi 966260.

Gene expression databases

Genevestigatori P43574.

Family and domain databases

Gene3Di 3.30.50.10. 1 hit.
InterProi IPR013860. DUF1752_fun.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF08550. DUF1752. 1 hit.
PF00320. GATA. 1 hit.
[Graphical view ]
PRINTSi PR00619. GATAZNFINGER.
SMARTi SM00401. ZnF_GATA. 1 hit.
[Graphical view ]
PROSITEi PS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae."
    Coffman J.A., Rai R., Cunningham T., Svetlov V., Cooper T.G.
    Mol. Cell. Biol. 16:847-858(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-399 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-262 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262; SER-270; THR-369 AND SER-399, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAT1_YEAST
AccessioniPrimary (citable) accession number: P43574
Secondary accession number(s): D6VTK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1180 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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