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P43574

- GAT1_YEAST

UniProt

P43574 - GAT1_YEAST

Protein

Transcriptional regulatory protein GAT1

Gene

GAT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Positive regulator of multiple nitrogen catabolic genes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri310 – 33425GATA-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
    2. RNA polymerase II transcription factor binding transcription factor activity Source: SGD
    3. sequence-specific DNA binding Source: SGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. nitrogen catabolite activation of transcription Source: SGD
    2. nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: GOC
    4. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30439-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein GAT1
    Gene namesi
    Name:GAT1
    Ordered Locus Names:YFL021W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL021w.
    SGDiS000001873. GAT1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 510510Transcriptional regulatory protein GAT1PRO_0000083484Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei167 – 1671Phosphoserine1 Publication
    Modified residuei262 – 2621Phosphoserine2 Publications
    Modified residuei270 – 2701Phosphoserine2 Publications
    Modified residuei369 – 3691Phosphothreonine1 Publication
    Modified residuei399 – 3991Phosphoserine2 Publications
    Modified residuei418 – 4181Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43574.
    PaxDbiP43574.

    Expressioni

    Gene expression databases

    GenevestigatoriP43574.

    Interactioni

    Protein-protein interaction databases

    BioGridi31125. 34 interactions.
    DIPiDIP-4276N.
    IntActiP43574. 7 interactions.
    MINTiMINT-525395.
    STRINGi4932.YFL021W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43574.
    SMRiP43574. Positions 310-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi151 – 1588Poly-Asp

    Sequence similaritiesi

    Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri310 – 33425GATA-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5641.
    GeneTreeiENSGT00750000117454.
    KOiK09184.
    OrthoDBiEOG7TQVCN.

    Family and domain databases

    Gene3Di3.30.50.10. 1 hit.
    InterProiIPR013860. DUF1752_fun.
    IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF08550. DUF1752. 1 hit.
    PF00320. GATA. 1 hit.
    [Graphical view]
    PRINTSiPR00619. GATAZNFINGER.
    SMARTiSM00401. ZnF_GATA. 1 hit.
    [Graphical view]
    PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
    PS50114. GATA_ZN_FINGER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43574-1 [UniParc]FASTAAdd to Basket

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    MHVFFPLLFR PSPVLFIACA YIYIDIYIHC TRCTVVNITM STNRVPNLDP    50
    DLNLNKEIWD LYSSAQKILP DSNRILNLSW RLHNRTSFHR INRIMQHSNS 100
    IMDFSASPFA SGVNAAGPGN NDLDDTDTDN QQFFLSDMNL NGSSVFENVF 150
    DDDDDDDDVE THSIVHSDLL NDMDSASQRA SHNASGFPNF LDTSCSSSFD 200
    DHFIFTNNLP FLNNNSINNN HSHNSSHNNN SPSIANNTNA NTNTNTSAST 250
    NTNSPLLRRN PSPSIVKPGS RRNSSVRKKK PALKKIKSST SVQSSATPPS 300
    NTSSNPDIKC SNCTTSTTPL WRKDPKGLPL CNACGLFLKL HGVTRPLSLK 350
    TDIIKKRQRS STKINNNITP PPSSSLNPGA AGKKKNYTAS VAASKRKNSL 400
    NIVAPLKSQD IPIPKIASPS IPQYLRSNTR HHLSSSVPIE AETFSSFRPD 450
    MNMTMNMNLH NASTSSFNNE AFWKPLDSAI DHHSGDTNPN SNMNTTPNGN 500
    LSLDWLNLNL 510
    Length:510
    Mass (Da):56,328
    Last modified:November 1, 1995 - v1
    Checksum:i62D805E42695F35F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27344 Genomic DNA. Translation: AAB03516.1.
    D50617 Genomic DNA. Translation: BAA09217.1.
    BK006940 Genomic DNA. Translation: DAA12419.1.
    PIRiS56233.
    RefSeqiNP_116632.1. NM_001179945.1.

    Genome annotation databases

    EnsemblFungiiYFL021W; YFL021W; YFL021W.
    GeneIDi850523.
    KEGGisce:YFL021W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27344 Genomic DNA. Translation: AAB03516.1 .
    D50617 Genomic DNA. Translation: BAA09217.1 .
    BK006940 Genomic DNA. Translation: DAA12419.1 .
    PIRi S56233.
    RefSeqi NP_116632.1. NM_001179945.1.

    3D structure databases

    ProteinModelPortali P43574.
    SMRi P43574. Positions 310-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31125. 34 interactions.
    DIPi DIP-4276N.
    IntActi P43574. 7 interactions.
    MINTi MINT-525395.
    STRINGi 4932.YFL021W.

    Proteomic databases

    MaxQBi P43574.
    PaxDbi P43574.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL021W ; YFL021W ; YFL021W .
    GeneIDi 850523.
    KEGGi sce:YFL021W.

    Organism-specific databases

    CYGDi YFL021w.
    SGDi S000001873. GAT1.

    Phylogenomic databases

    eggNOGi COG5641.
    GeneTreei ENSGT00750000117454.
    KOi K09184.
    OrthoDBi EOG7TQVCN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30439-MONOMER.

    Miscellaneous databases

    NextBioi 966260.

    Gene expression databases

    Genevestigatori P43574.

    Family and domain databases

    Gene3Di 3.30.50.10. 1 hit.
    InterProi IPR013860. DUF1752_fun.
    IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF08550. DUF1752. 1 hit.
    PF00320. GATA. 1 hit.
    [Graphical view ]
    PRINTSi PR00619. GATAZNFINGER.
    SMARTi SM00401. ZnF_GATA. 1 hit.
    [Graphical view ]
    PROSITEi PS00344. GATA_ZN_FINGER_1. 1 hit.
    PS50114. GATA_ZN_FINGER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae."
      Coffman J.A., Rai R., Cunningham T., Svetlov V., Cooper T.G.
      Mol. Cell. Biol. 16:847-858(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-399 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-262 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262; SER-270; THR-369 AND SER-399, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGAT1_YEAST
    AccessioniPrimary (citable) accession number: P43574
    Secondary accession number(s): D6VTK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1180 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3