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P43574 (GAT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulatory protein GAT1
Gene names
Name:GAT1
Ordered Locus Names:YFL021W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulator of multiple nitrogen catabolic genes.

Subcellular location

Nucleus Potential.

Miscellaneous

Present with 1180 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 GATA-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510Transcriptional regulatory protein GAT1
PRO_0000083484

Regions

Zinc finger310 – 33425GATA-type
Compositional bias151 – 1588Poly-Asp

Amino acid modifications

Modified residue1671Phosphoserine Ref.6
Modified residue2621Phosphoserine Ref.6 Ref.7
Modified residue2701Phosphoserine Ref.5 Ref.7
Modified residue3691Phosphothreonine Ref.7
Modified residue3991Phosphoserine Ref.5 Ref.7
Modified residue4181Phosphoserine Ref.5 Ref.6

Sequences

Sequence LengthMass (Da)Tools
P43574 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 62D805E42695F35F

FASTA51056,328
        10         20         30         40         50         60 
MHVFFPLLFR PSPVLFIACA YIYIDIYIHC TRCTVVNITM STNRVPNLDP DLNLNKEIWD 

        70         80         90        100        110        120 
LYSSAQKILP DSNRILNLSW RLHNRTSFHR INRIMQHSNS IMDFSASPFA SGVNAAGPGN 

       130        140        150        160        170        180 
NDLDDTDTDN QQFFLSDMNL NGSSVFENVF DDDDDDDDVE THSIVHSDLL NDMDSASQRA 

       190        200        210        220        230        240 
SHNASGFPNF LDTSCSSSFD DHFIFTNNLP FLNNNSINNN HSHNSSHNNN SPSIANNTNA 

       250        260        270        280        290        300 
NTNTNTSAST NTNSPLLRRN PSPSIVKPGS RRNSSVRKKK PALKKIKSST SVQSSATPPS 

       310        320        330        340        350        360 
NTSSNPDIKC SNCTTSTTPL WRKDPKGLPL CNACGLFLKL HGVTRPLSLK TDIIKKRQRS 

       370        380        390        400        410        420 
STKINNNITP PPSSSLNPGA AGKKKNYTAS VAASKRKNSL NIVAPLKSQD IPIPKIASPS 

       430        440        450        460        470        480 
IPQYLRSNTR HHLSSSVPIE AETFSSFRPD MNMTMNMNLH NASTSSFNNE AFWKPLDSAI 

       490        500        510 
DHHSGDTNPN SNMNTTPNGN LSLDWLNLNL 

« Hide

References

« Hide 'large scale' references
[1]"Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae."
Coffman J.A., Rai R., Cunningham T., Svetlov V., Cooper T.G.
Mol. Cell. Biol. 16:847-858(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-399 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-262 AND SER-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262; SER-270; THR-369 AND SER-399, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U27344 Genomic DNA. Translation: AAB03516.1.
D50617 Genomic DNA. Translation: BAA09217.1.
BK006940 Genomic DNA. Translation: DAA12419.1.
PIRS56233.
RefSeqNP_116632.1. NM_001179945.1.

3D structure databases

ProteinModelPortalP43574.
SMRP43574. Positions 310-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31125. 34 interactions.
DIPDIP-4276N.
IntActP43574. 7 interactions.
MINTMINT-525395.
STRING4932.YFL021W.

Proteomic databases

MaxQBP43574.
PaxDbP43574.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL021W; YFL021W; YFL021W.
GeneID850523.
KEGGsce:YFL021W.

Organism-specific databases

CYGDYFL021w.
SGDS000001873. GAT1.

Phylogenomic databases

eggNOGCOG5641.
GeneTreeENSGT00750000117454.
KOK09184.
OrthoDBEOG7TQVCN.

Enzyme and pathway databases

BioCycYEAST:G3O-30439-MONOMER.

Gene expression databases

GenevestigatorP43574.

Family and domain databases

Gene3D3.30.50.10. 1 hit.
InterProIPR013860. DUF1752_fun.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF08550. DUF1752. 1 hit.
PF00320. GATA. 1 hit.
[Graphical view]
PRINTSPR00619. GATAZNFINGER.
SMARTSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966260.

Entry information

Entry nameGAT1_YEAST
AccessionPrimary (citable) accession number: P43574
Secondary accession number(s): D6VTK9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families