P43574 (GAT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein GAT1

Gene names

Name:	GAT1
Ordered Locus Names:	YFL021W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of multiple nitrogen catabolic genes.
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 1180 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 GATA-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen catabolite activation of transcription Inferred from mutant phenotype Ref.1. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	sequence-specific DNA binding Inferred from direct assay. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Transcriptional regulatory protein GAT1		PRO_0000083484
Regions
	Zinc finger	310 – 334	25	GATA-type
	Compositional bias	151 – 158	8	Poly-Asp
Amino acid modifications
	Modified residue	167	1	Phosphoserine Ref.8
	Modified residue	262	1	Phosphoserine Ref.8
	Modified residue	270	1	Phosphoserine Ref.6
	Modified residue	288	1	Phosphoserine Ref.7
	Modified residue	369	1	Phosphothreonine Ref.8
	Modified residue	399	1	Phosphoserine Ref.6
	Modified residue	418	1	Phosphoserine Ref.5 Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P43574 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 62D805E42695F35F	FASTA	510	56,328
	10 20 30 40 50 60 MHVFFPLLFR PSPVLFIACA YIYIDIYIHC TRCTVVNITM STNRVPNLDP DLNLNKEIWD 70 80 90 100 110 120 LYSSAQKILP DSNRILNLSW RLHNRTSFHR INRIMQHSNS IMDFSASPFA SGVNAAGPGN 130 140 150 160 170 180 NDLDDTDTDN QQFFLSDMNL NGSSVFENVF DDDDDDDDVE THSIVHSDLL NDMDSASQRA 190 200 210 220 230 240 SHNASGFPNF LDTSCSSSFD DHFIFTNNLP FLNNNSINNN HSHNSSHNNN SPSIANNTNA 250 260 270 280 290 300 NTNTNTSAST NTNSPLLRRN PSPSIVKPGS RRNSSVRKKK PALKKIKSST SVQSSATPPS 310 320 330 340 350 360 NTSSNPDIKC SNCTTSTTPL WRKDPKGLPL CNACGLFLKL HGVTRPLSLK TDIIKKRQRS 370 380 390 400 410 420 STKINNNITP PPSSSLNPGA AGKKKNYTAS VAASKRKNSL NIVAPLKSQD IPIPKIASPS 430 440 450 460 470 480 IPQYLRSNTR HHLSSSVPIE AETFSSFRPD MNMTMNMNLH NASTSSFNNE AFWKPLDSAI 490 500 510 DHHSGDTNPN SNMNTTPNGN LSLDWLNLNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae." Coffman J.A., Rai R., Cunningham T., Svetlov V., Cooper T.G. Mol. Cell. Biol. 16:847-858(1996) [PubMed: 8622686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-418, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-399 AND SER-418, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-262; THR-369 AND SER-418, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27344 Genomic DNA. Translation: AAB03516.1. D50617 Genomic DNA. Translation: BAA09217.1. BK006940 Genomic DNA. Translation: DAA12419.1.
PIR	S56233.
RefSeq	NP_116632.1. NM_001179945.1.
3D structure databases
ProteinModelPortal	P43574.
SMR	P43574. Positions 310-349.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4276N.
IntAct	P43574. 6 interactions.
MINT	MINT-525395.
STRING	P43574.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL021W; YFL021W; YFL021W.
GeneID	850523.
KEGG	sce:YFL021W.
NMPDR	fig|4932.3.peg.2260.
Organism-specific databases
CYGD	YFL021w.
SGD	S000001873. GAT1.
Phylogenomic databases
eggNOG	fuNOG11971.
GeneTree	EFGT00050000000802.
OrthoDB	EOG4XPTQ0.
Gene expression databases
ArrayExpress	P43574.
Genevestigator	P43574.
GermOnline	YFL021W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013860. DUF1752_fun. IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
KO	K09184.
Pfam	PF08550. DUF1752. 1 hit. PF00320. GATA. 1 hit. [Graphical view]
PRINTS	PR00619. GATAZNFINGER.
SMART	SM00401. ZnF_GATA. 1 hit. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. 1 hit. PS50114. GATA_ZN_FINGER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966260.

Entry information

Entry name

GAT1_YEAST

Accession

Primary (citable) accession number: P43574
Secondary accession number(s): D6VTK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program