Transcriptional regulatory protein GAT1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P43574 (GAT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein GAT1

Gene names

Name:	GAT1
Ordered Locus Names:	YFL021W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of multiple nitrogen catabolic genes.
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 1180 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 GATA-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrogen catabolite activation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 7568152. Source: SGD
Cellular_component	cytosol Inferred from direct assay PubMed 10799523. Source: SGD nucleus Inferred from direct assay PubMed 10799523. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from mutant phenotype PubMed 9106207. Source: SGD RNA polymerase II transcription factor binding transcription factor activity Inferred from mutant phenotype PubMed 19380492. Source: SGD sequence-specific DNA binding Inferred from direct assay PubMed 19111667 PubMed 19158363. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 510

510

Transcriptional regulatory protein GAT1

PRO_0000083484

Regions

Zinc finger

310 – 334

GATA-type

Compositional bias

151 – 158

Poly-Asp

Amino acid modifications

Modified residue

167

Phosphoserine Ref.8

Modified residue

262

Phosphoserine Ref.8

Modified residue

270

Phosphoserine Ref.6

Modified residue

288

Phosphoserine Ref.7

Modified residue

369

Phosphothreonine Ref.8

Modified residue

399

Phosphoserine Ref.6

Modified residue

418

Phosphoserine Ref.5 Ref.6 Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P43574 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 62D805E42695F35F
Show »

FASTA

510

56,328

        10         20         30         40         50         60 
MHVFFPLLFR PSPVLFIACA YIYIDIYIHC TRCTVVNITM STNRVPNLDP DLNLNKEIWD 

        70         80         90        100        110        120 
LYSSAQKILP DSNRILNLSW RLHNRTSFHR INRIMQHSNS IMDFSASPFA SGVNAAGPGN 

       130        140        150        160        170        180 
NDLDDTDTDN QQFFLSDMNL NGSSVFENVF DDDDDDDDVE THSIVHSDLL NDMDSASQRA 

       190        200        210        220        230        240 
SHNASGFPNF LDTSCSSSFD DHFIFTNNLP FLNNNSINNN HSHNSSHNNN SPSIANNTNA 

       250        260        270        280        290        300 
NTNTNTSAST NTNSPLLRRN PSPSIVKPGS RRNSSVRKKK PALKKIKSST SVQSSATPPS 

       310        320        330        340        350        360 
NTSSNPDIKC SNCTTSTTPL WRKDPKGLPL CNACGLFLKL HGVTRPLSLK TDIIKKRQRS 

       370        380        390        400        410        420 
STKINNNITP PPSSSLNPGA AGKKKNYTAS VAASKRKNSL NIVAPLKSQD IPIPKIASPS 

       430        440        450        460        470        480 
IPQYLRSNTR HHLSSSVPIE AETFSSFRPD MNMTMNMNLH NASTSSFNNE AFWKPLDSAI 

       490        500        510 
DHHSGDTNPN SNMNTTPNGN LSLDWLNLNL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae." Coffman J.A., Rai R., Cunningham T., Svetlov V., Cooper T.G. Mol. Cell. Biol. 16:847-858(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-418, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-399 AND SER-418, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-262; THR-369 AND SER-418, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27344 Genomic DNA. Translation: AAB03516.1. D50617 Genomic DNA. Translation: BAA09217.1. BK006940 Genomic DNA. Translation: DAA12419.1.
PIR	S56233.
RefSeq	NP_116632.1. NM_001179945.1.
3D structure databases
ProteinModelPortal	P43574.
SMR	P43574. Positions 245-358.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4276N.
IntAct	P43574. 6 interactions.
MINT	MINT-525395.
STRING	4932.YFL021W.
Proteomic databases
PaxDb	P43574.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL021W; YFL021W; YFL021W.
GeneID	850523.
KEGG	sce:YFL021W.
Organism-specific databases
CYGD	YFL021w.
SGD	S000001873. GAT1.
Phylogenomic databases
eggNOG	COG5641.
GeneTree	ENSGT00550000074470.
KO	K09184.
OrthoDB	EOG4XPTQ0.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30439-MONOMER.
Gene expression databases
Genevestigator	P43574.
GermOnline	YFL021W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.50.10. 1 hit.
InterPro	IPR013860. DUF1752_fun. IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF08550. DUF1752. 1 hit. PF00320. GATA. 1 hit. [Graphical view]
PRINTS	PR00619. GATAZNFINGER.
SMART	SM00401. ZnF_GATA. 1 hit. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. 1 hit. PS50114. GATA_ZN_FINGER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966260.

Entry information

Entry name

GAT1_YEAST

Accession

Primary (citable) accession number: P43574
Secondary accession number(s): D6VTK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents