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Protein

Bud site selection protein 27

Gene

BUD27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in gene expression controlled by TOR kinase and nutrient signaling. May also be involved in positioning the proximal bud pole signal.3 Publications

GO - Molecular functioni

GO - Biological processi

  • formation of translation preinitiation complex Source: SGD
  • RNA Polymerase I assembly Source: SGD
  • RNA Polymerase II core complex assembly Source: SGD
  • RNA Polymerase III assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 27
Gene namesi
Name:BUD27
Synonyms:URI
Ordered Locus Names:YFL023W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL023W.
SGDiS000001871. BUD27.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: GO_Central
  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001536931 – 796Bud site selection protein 27Add BLAST796

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei580PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43573.
PRIDEiP43573.

PTM databases

iPTMnetiP43573.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FPR1P200812EBI-22787,EBI-6961
SSB1P114843EBI-22787,EBI-8627
TEF2P029942EBI-22787,EBI-6314
TEF4P360083EBI-22787,EBI-6329
YKE2P525535EBI-22787,EBI-13260

Protein-protein interaction databases

BioGridi31123. 266 interactors.
DIPiDIP-4929N.
IntActiP43573. 21 interactors.
MINTiMINT-474511.

Structurei

3D structure databases

ProteinModelPortaliP43573.
SMRiP43573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili81 – 121Sequence analysisAdd BLAST41

Sequence similaritiesi

Belongs to the prefoldin subunit alpha family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00730000114241.
HOGENOMiHOG000095278.
InParanoidiP43573.
KOiK17560.
OMAiREPAIND.
OrthoDBiEOG092C0ZXG.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR024325. DUF3835.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PfamiPF12927. DUF3835. 1 hit.
PF02996. Prefoldin. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequencei

Sequence statusi: Complete.

P43573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLLAASVES TLKNLQDKRN FLSEQREHYI DIRSRLVRFI NDNDDGEEEG
60 70 80 90 100
EGQGMVFGDI IISTSKIYLS LGYEYYVEKT KEEAITFVDD KLKLMEDAIE
110 120 130 140 150
QFNLKIEEAK KTLDNLNHME DGNGIEEDEA NNDEDFLPSM EIREELDDEG
160 170 180 190 200
NVISSSVTPT TKQPSQSNSK KEQTPAVGPK EKGLAKEKKS KSFEENLKGK
210 220 230 240 250
LLKRNDEVKK KVQPSKVDTE NVYTFADLVQ QMDQQDELED GYIETDEINY
260 270 280 290 300
DYDAFENSNF KVNDNYEEDD EDEDEEEYLN HSIIPGFEAQ SSFLQQIQRL
310 320 330 340 350
RAQKQSQDHE REEGDVNKSL KPILKKSSFA ENSDKKQKKK QVGFASSLEI
360 370 380 390 400
HEVENLKEEN KRQMQSFAVP MYETQESTGI ANKMTSDEFD GDLFAKMLGV
410 420 430 440 450
QEADEVHEKY KEELINQERL EGEASRSNRR TRVSRFRKDR ASKKENTLST
460 470 480 490 500
FKQETTRSVE NEVVEKEPVV GDIIEKEPVV GDVIEKEPVV GDVIEKEPAV
510 520 530 540 550
TDIVEREPAV NDIVERKPVV GDIIEKEPTI NDIVEKEPEI NSKSEFETPF
560 570 580 590 600
KKKKLKSLQK PRSSKSMKKK FDPKILENIS DDDYDDDDDG NKKLLSNKSK
610 620 630 640 650
NNTDEQDKFP SKIQEVSRSM AKTGATVGSE PVRITNVDYH ALGGNLDDMV
660 670 680 690 700
KAYSLGLYDD DLEEDPGTIV EKLEDFKEYN KQVELLRDEI RDFQLENKPV
710 720 730 740 750
TMEEEENDGN VMNDIIEHEF PESYTNDEDE VALHPGRLQE EVAIEYRRLK
760 770 780 790
EATASKWQSS SPAAHTEGEL EPIDKFGNPV KTSRFRSQRL HMDSKP
Length:796
Mass (Da):91,422
Last modified:November 1, 1995 - v1
Checksum:i7E2EE2CEB4708BC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09215.1.
BK006940 Genomic DNA. Translation: DAA12417.1.
PIRiS56231.
RefSeqiNP_683715.1. NM_001179943.1.

Genome annotation databases

EnsemblFungiiBAA09215; BAA09215; BAA09215.
YFL023W; YFL023W; YFL023W.
GeneIDi850521.
KEGGisce:YFL023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09215.1.
BK006940 Genomic DNA. Translation: DAA12417.1.
PIRiS56231.
RefSeqiNP_683715.1. NM_001179943.1.

3D structure databases

ProteinModelPortaliP43573.
SMRiP43573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31123. 266 interactors.
DIPiDIP-4929N.
IntActiP43573. 21 interactors.
MINTiMINT-474511.

PTM databases

iPTMnetiP43573.

Proteomic databases

MaxQBiP43573.
PRIDEiP43573.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09215; BAA09215; BAA09215.
YFL023W; YFL023W; YFL023W.
GeneIDi850521.
KEGGisce:YFL023W.

Organism-specific databases

EuPathDBiFungiDB:YFL023W.
SGDiS000001871. BUD27.

Phylogenomic databases

GeneTreeiENSGT00730000114241.
HOGENOMiHOG000095278.
InParanoidiP43573.
KOiK17560.
OMAiREPAIND.
OrthoDBiEOG092C0ZXG.

Enzyme and pathway databases

BioCyciYEAST:G3O-30437-MONOMER.

Miscellaneous databases

PROiP43573.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR024325. DUF3835.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PfamiPF12927. DUF3835. 1 hit.
PF02996. Prefoldin. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBUD27_YEAST
AccessioniPrimary (citable) accession number: P43573
Secondary accession number(s): D6VTK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication
Deletion leads to a K1 killer toxin hypersensitivity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.