Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43573

- BUD27_YEAST

UniProt

P43573 - BUD27_YEAST

Protein

Bud site selection protein 27

Gene

BUD27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in gene expression controlled by TOR kinase and nutrient signaling. May also be involved in positioning the proximal bud pole signal.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. formation of translation preinitiation complex Source: SGD
    2. protein folding Source: InterPro
    3. RNA Polymerase I assembly Source: SGD
    4. RNA Polymerase II core complex assembly Source: SGD
    5. RNA Polymerase III assembly Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30437-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bud site selection protein 27
    Gene namesi
    Name:BUD27
    Synonyms:URI
    Ordered Locus Names:YFL023W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL023w.
    SGDiS000001871. BUD27.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. prefoldin complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 796796Bud site selection protein 27PRO_0000153693Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei580 – 5801Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43573.
    PaxDbiP43573.
    PeptideAtlasiP43573.

    Expressioni

    Gene expression databases

    GenevestigatoriP43573.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FPR1P200812EBI-22787,EBI-6961
    SSB1P114843EBI-22787,EBI-8627
    TEF2P029942EBI-22787,EBI-6314
    TEF4P360083EBI-22787,EBI-6329
    YKE2P525535EBI-22787,EBI-13260

    Protein-protein interaction databases

    BioGridi31123. 256 interactions.
    DIPiDIP-4929N.
    IntActiP43573. 20 interactions.
    MINTiMINT-474511.
    STRINGi4932.YFL023W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili81 – 12141Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prefoldin subunit alpha family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG41103.
    GeneTreeiENSGT00730000114241.
    HOGENOMiHOG000095278.
    KOiK17560.
    OMAiREPAIND.
    OrthoDBiEOG7D59ZS.

    Family and domain databases

    Gene3Di1.10.287.370. 1 hit.
    InterProiIPR024325. DUF3835.
    IPR009053. Prefoldin.
    IPR004127. Prefoldin_subunit_alpha.
    [Graphical view]
    PfamiPF12927. DUF3835. 1 hit.
    PF02996. Prefoldin. 1 hit.
    [Graphical view]
    SUPFAMiSSF46579. SSF46579. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43573-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLLAASVES TLKNLQDKRN FLSEQREHYI DIRSRLVRFI NDNDDGEEEG    50
    EGQGMVFGDI IISTSKIYLS LGYEYYVEKT KEEAITFVDD KLKLMEDAIE 100
    QFNLKIEEAK KTLDNLNHME DGNGIEEDEA NNDEDFLPSM EIREELDDEG 150
    NVISSSVTPT TKQPSQSNSK KEQTPAVGPK EKGLAKEKKS KSFEENLKGK 200
    LLKRNDEVKK KVQPSKVDTE NVYTFADLVQ QMDQQDELED GYIETDEINY 250
    DYDAFENSNF KVNDNYEEDD EDEDEEEYLN HSIIPGFEAQ SSFLQQIQRL 300
    RAQKQSQDHE REEGDVNKSL KPILKKSSFA ENSDKKQKKK QVGFASSLEI 350
    HEVENLKEEN KRQMQSFAVP MYETQESTGI ANKMTSDEFD GDLFAKMLGV 400
    QEADEVHEKY KEELINQERL EGEASRSNRR TRVSRFRKDR ASKKENTLST 450
    FKQETTRSVE NEVVEKEPVV GDIIEKEPVV GDVIEKEPVV GDVIEKEPAV 500
    TDIVEREPAV NDIVERKPVV GDIIEKEPTI NDIVEKEPEI NSKSEFETPF 550
    KKKKLKSLQK PRSSKSMKKK FDPKILENIS DDDYDDDDDG NKKLLSNKSK 600
    NNTDEQDKFP SKIQEVSRSM AKTGATVGSE PVRITNVDYH ALGGNLDDMV 650
    KAYSLGLYDD DLEEDPGTIV EKLEDFKEYN KQVELLRDEI RDFQLENKPV 700
    TMEEEENDGN VMNDIIEHEF PESYTNDEDE VALHPGRLQE EVAIEYRRLK 750
    EATASKWQSS SPAAHTEGEL EPIDKFGNPV KTSRFRSQRL HMDSKP 796
    Length:796
    Mass (Da):91,422
    Last modified:November 1, 1995 - v1
    Checksum:i7E2EE2CEB4708BC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09215.1.
    BK006940 Genomic DNA. Translation: DAA12417.1.
    PIRiS56231.
    RefSeqiNP_683715.1. NM_001179943.1.

    Genome annotation databases

    EnsemblFungiiYFL023W; YFL023W; YFL023W.
    GeneIDi850521.
    KEGGisce:YFL023W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09215.1 .
    BK006940 Genomic DNA. Translation: DAA12417.1 .
    PIRi S56231.
    RefSeqi NP_683715.1. NM_001179943.1.

    3D structure databases

    ProteinModelPortali P43573.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31123. 256 interactions.
    DIPi DIP-4929N.
    IntActi P43573. 20 interactions.
    MINTi MINT-474511.
    STRINGi 4932.YFL023W.

    Proteomic databases

    MaxQBi P43573.
    PaxDbi P43573.
    PeptideAtlasi P43573.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL023W ; YFL023W ; YFL023W .
    GeneIDi 850521.
    KEGGi sce:YFL023W.

    Organism-specific databases

    CYGDi YFL023w.
    SGDi S000001871. BUD27.

    Phylogenomic databases

    eggNOGi NOG41103.
    GeneTreei ENSGT00730000114241.
    HOGENOMi HOG000095278.
    KOi K17560.
    OMAi REPAIND.
    OrthoDBi EOG7D59ZS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30437-MONOMER.

    Miscellaneous databases

    NextBioi 966254.

    Gene expression databases

    Genevestigatori P43573.

    Family and domain databases

    Gene3Di 1.10.287.370. 1 hit.
    InterProi IPR024325. DUF3835.
    IPR009053. Prefoldin.
    IPR004127. Prefoldin_subunit_alpha.
    [Graphical view ]
    Pfami PF12927. DUF3835. 1 hit.
    PF02996. Prefoldin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46579. SSF46579. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
      Ni L., Snyder M.
      Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin."
      Page N., Gerard-Vincent M., Menard P., Beaulieu M., Azuma M., Dijkgraaf G.J.P., Li H., Marcoux J., Nguyen T., Dowse T., Sdicu A.-M., Bussey H.
      Genetics 163:875-894(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI."
      Gstaiger M., Luke B., Hess D., Oakeley E.J., Wirbelauer C., Blondel M., Vigneron M., Peter M., Krek W.
      Science 302:1208-1212(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBUD27_YEAST
    AccessioniPrimary (citable) accession number: P43573
    Secondary accession number(s): D6VTK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1300 molecules/cell in log phase SD medium.1 Publication
    Deletion leads to a K1 killer toxin hypersensitivity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3