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P43573 (BUD27_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bud site selection protein 27
Gene names
Name:BUD27
Synonyms:URI
Ordered Locus Names:YFL023W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length796 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in gene expression controlled by TOR kinase and nutrient signaling. May also be involved in positioning the proximal bud pole signal. Ref.3 Ref.4 Ref.7

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.

Deletion leads to a K1 killer toxin hypersensitivity.

Sequence similarities

Belongs to the prefoldin subunit alpha family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 796796Bud site selection protein 27
PRO_0000153693

Regions

Coiled coil81 – 12141 Potential

Amino acid modifications

Modified residue5801Phosphoserine Ref.8 Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P43573 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7E2EE2CEB4708BC3

FASTA79691,422
        10         20         30         40         50         60 
MDLLAASVES TLKNLQDKRN FLSEQREHYI DIRSRLVRFI NDNDDGEEEG EGQGMVFGDI 

        70         80         90        100        110        120 
IISTSKIYLS LGYEYYVEKT KEEAITFVDD KLKLMEDAIE QFNLKIEEAK KTLDNLNHME 

       130        140        150        160        170        180 
DGNGIEEDEA NNDEDFLPSM EIREELDDEG NVISSSVTPT TKQPSQSNSK KEQTPAVGPK 

       190        200        210        220        230        240 
EKGLAKEKKS KSFEENLKGK LLKRNDEVKK KVQPSKVDTE NVYTFADLVQ QMDQQDELED 

       250        260        270        280        290        300 
GYIETDEINY DYDAFENSNF KVNDNYEEDD EDEDEEEYLN HSIIPGFEAQ SSFLQQIQRL 

       310        320        330        340        350        360 
RAQKQSQDHE REEGDVNKSL KPILKKSSFA ENSDKKQKKK QVGFASSLEI HEVENLKEEN 

       370        380        390        400        410        420 
KRQMQSFAVP MYETQESTGI ANKMTSDEFD GDLFAKMLGV QEADEVHEKY KEELINQERL 

       430        440        450        460        470        480 
EGEASRSNRR TRVSRFRKDR ASKKENTLST FKQETTRSVE NEVVEKEPVV GDIIEKEPVV 

       490        500        510        520        530        540 
GDVIEKEPVV GDVIEKEPAV TDIVEREPAV NDIVERKPVV GDIIEKEPTI NDIVEKEPEI 

       550        560        570        580        590        600 
NSKSEFETPF KKKKLKSLQK PRSSKSMKKK FDPKILENIS DDDYDDDDDG NKKLLSNKSK 

       610        620        630        640        650        660 
NNTDEQDKFP SKIQEVSRSM AKTGATVGSE PVRITNVDYH ALGGNLDDMV KAYSLGLYDD 

       670        680        690        700        710        720 
DLEEDPGTIV EKLEDFKEYN KQVELLRDEI RDFQLENKPV TMEEEENDGN VMNDIIEHEF 

       730        740        750        760        770        780 
PESYTNDEDE VALHPGRLQE EVAIEYRRLK EATASKWQSS SPAAHTEGEL EPIDKFGNPV 

       790 
KTSRFRSQRL HMDSKP 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
Ni L., Snyder M.
Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin."
Page N., Gerard-Vincent M., Menard P., Beaulieu M., Azuma M., Dijkgraaf G.J.P., Li H., Marcoux J., Nguyen T., Dowse T., Sdicu A.-M., Bussey H.
Genetics 163:875-894(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI."
Gstaiger M., Luke B., Hess D., Oakeley E.J., Wirbelauer C., Blondel M., Vigneron M., Peter M., Krek W.
Science 302:1208-1212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50617 Genomic DNA. Translation: BAA09215.1.
BK006940 Genomic DNA. Translation: DAA12417.1.
PIRS56231.
RefSeqNP_683715.1. NM_001179943.1.

3D structure databases

ProteinModelPortalP43573.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31123. 256 interactions.
DIPDIP-4929N.
IntActP43573. 20 interactions.
MINTMINT-474511.
STRING4932.YFL023W.

Proteomic databases

MaxQBP43573.
PaxDbP43573.
PeptideAtlasP43573.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL023W; YFL023W; YFL023W.
GeneID850521.
KEGGsce:YFL023W.

Organism-specific databases

CYGDYFL023w.
SGDS000001871. BUD27.

Phylogenomic databases

eggNOGNOG41103.
GeneTreeENSGT00730000114241.
HOGENOMHOG000095278.
KOK17560.
OMAREPAIND.
OrthoDBEOG7D59ZS.

Enzyme and pathway databases

BioCycYEAST:G3O-30437-MONOMER.

Gene expression databases

GenevestigatorP43573.

Family and domain databases

Gene3D1.10.287.370. 1 hit.
InterProIPR024325. DUF3835.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PfamPF12927. DUF3835. 1 hit.
PF02996. Prefoldin. 1 hit.
[Graphical view]
SUPFAMSSF46579. SSF46579. 1 hit.
ProtoNetSearch...

Other

NextBio966254.

Entry information

Entry nameBUD27_YEAST
AccessionPrimary (citable) accession number: P43573
Secondary accession number(s): D6VTK7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families