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P43573

- BUD27_YEAST

UniProt

P43573 - BUD27_YEAST

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Protein

Bud site selection protein 27

Gene

BUD27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in gene expression controlled by TOR kinase and nutrient signaling. May also be involved in positioning the proximal bud pole signal.3 Publications

GO - Biological processi

  1. formation of translation preinitiation complex Source: SGD
  2. protein folding Source: InterPro
  3. RNA Polymerase I assembly Source: SGD
  4. RNA Polymerase II core complex assembly Source: SGD
  5. RNA Polymerase III assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 27
Gene namesi
Name:BUD27
Synonyms:URI
Ordered Locus Names:YFL023W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL023w.
SGDiS000001871. BUD27.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. prefoldin complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 796796Bud site selection protein 27PRO_0000153693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei580 – 5801Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43573.
PaxDbiP43573.
PeptideAtlasiP43573.

Expressioni

Gene expression databases

GenevestigatoriP43573.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FPR1P200812EBI-22787,EBI-6961
SSB1P114843EBI-22787,EBI-8627
TEF2P029942EBI-22787,EBI-6314
TEF4P360083EBI-22787,EBI-6329
YKE2P525535EBI-22787,EBI-13260

Protein-protein interaction databases

BioGridi31123. 256 interactions.
DIPiDIP-4929N.
IntActiP43573. 20 interactions.
MINTiMINT-474511.
STRINGi4932.YFL023W.

Structurei

3D structure databases

ProteinModelPortaliP43573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili81 – 12141Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the prefoldin subunit alpha family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG41103.
GeneTreeiENSGT00730000114241.
HOGENOMiHOG000095278.
InParanoidiP43573.
KOiK17560.
OMAiREPAIND.
OrthoDBiEOG7D59ZS.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR024325. DUF3835.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PfamiPF12927. DUF3835. 1 hit.
PF02996. Prefoldin. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequencei

Sequence statusi: Complete.

P43573-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLLAASVES TLKNLQDKRN FLSEQREHYI DIRSRLVRFI NDNDDGEEEG
60 70 80 90 100
EGQGMVFGDI IISTSKIYLS LGYEYYVEKT KEEAITFVDD KLKLMEDAIE
110 120 130 140 150
QFNLKIEEAK KTLDNLNHME DGNGIEEDEA NNDEDFLPSM EIREELDDEG
160 170 180 190 200
NVISSSVTPT TKQPSQSNSK KEQTPAVGPK EKGLAKEKKS KSFEENLKGK
210 220 230 240 250
LLKRNDEVKK KVQPSKVDTE NVYTFADLVQ QMDQQDELED GYIETDEINY
260 270 280 290 300
DYDAFENSNF KVNDNYEEDD EDEDEEEYLN HSIIPGFEAQ SSFLQQIQRL
310 320 330 340 350
RAQKQSQDHE REEGDVNKSL KPILKKSSFA ENSDKKQKKK QVGFASSLEI
360 370 380 390 400
HEVENLKEEN KRQMQSFAVP MYETQESTGI ANKMTSDEFD GDLFAKMLGV
410 420 430 440 450
QEADEVHEKY KEELINQERL EGEASRSNRR TRVSRFRKDR ASKKENTLST
460 470 480 490 500
FKQETTRSVE NEVVEKEPVV GDIIEKEPVV GDVIEKEPVV GDVIEKEPAV
510 520 530 540 550
TDIVEREPAV NDIVERKPVV GDIIEKEPTI NDIVEKEPEI NSKSEFETPF
560 570 580 590 600
KKKKLKSLQK PRSSKSMKKK FDPKILENIS DDDYDDDDDG NKKLLSNKSK
610 620 630 640 650
NNTDEQDKFP SKIQEVSRSM AKTGATVGSE PVRITNVDYH ALGGNLDDMV
660 670 680 690 700
KAYSLGLYDD DLEEDPGTIV EKLEDFKEYN KQVELLRDEI RDFQLENKPV
710 720 730 740 750
TMEEEENDGN VMNDIIEHEF PESYTNDEDE VALHPGRLQE EVAIEYRRLK
760 770 780 790
EATASKWQSS SPAAHTEGEL EPIDKFGNPV KTSRFRSQRL HMDSKP
Length:796
Mass (Da):91,422
Last modified:November 1, 1995 - v1
Checksum:i7E2EE2CEB4708BC3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09215.1.
BK006940 Genomic DNA. Translation: DAA12417.1.
PIRiS56231.
RefSeqiNP_683715.1. NM_001179943.1.

Genome annotation databases

EnsemblFungiiYFL023W; YFL023W; YFL023W.
GeneIDi850521.
KEGGisce:YFL023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09215.1 .
BK006940 Genomic DNA. Translation: DAA12417.1 .
PIRi S56231.
RefSeqi NP_683715.1. NM_001179943.1.

3D structure databases

ProteinModelPortali P43573.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31123. 256 interactions.
DIPi DIP-4929N.
IntActi P43573. 20 interactions.
MINTi MINT-474511.
STRINGi 4932.YFL023W.

Proteomic databases

MaxQBi P43573.
PaxDbi P43573.
PeptideAtlasi P43573.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL023W ; YFL023W ; YFL023W .
GeneIDi 850521.
KEGGi sce:YFL023W.

Organism-specific databases

CYGDi YFL023w.
SGDi S000001871. BUD27.

Phylogenomic databases

eggNOGi NOG41103.
GeneTreei ENSGT00730000114241.
HOGENOMi HOG000095278.
InParanoidi P43573.
KOi K17560.
OMAi REPAIND.
OrthoDBi EOG7D59ZS.

Enzyme and pathway databases

BioCyci YEAST:G3O-30437-MONOMER.

Miscellaneous databases

NextBioi 966254.

Gene expression databases

Genevestigatori P43573.

Family and domain databases

Gene3Di 1.10.287.370. 1 hit.
InterProi IPR024325. DUF3835.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view ]
Pfami PF12927. DUF3835. 1 hit.
PF02996. Prefoldin. 1 hit.
[Graphical view ]
SUPFAMi SSF46579. SSF46579. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
    Ni L., Snyder M.
    Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin."
    Page N., Gerard-Vincent M., Menard P., Beaulieu M., Azuma M., Dijkgraaf G.J.P., Li H., Marcoux J., Nguyen T., Dowse T., Sdicu A.-M., Bussey H.
    Genetics 163:875-894(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI."
    Gstaiger M., Luke B., Hess D., Oakeley E.J., Wirbelauer C., Blondel M., Vigneron M., Peter M., Krek W.
    Science 302:1208-1212(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBUD27_YEAST
AccessioniPrimary (citable) accession number: P43573
Secondary accession number(s): D6VTK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication
Deletion leads to a K1 killer toxin hypersensitivity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3