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Protein

GPI inositol-deacylase

Gene

BST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins. Required for the transport of misfolded protein to the Golgi, although dipensable for the transport of many normal proteins.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei236By similarity1

GO - Molecular functioni

  • phosphatidylinositol deacylase activity Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • ER to Golgi vesicle-mediated transport Source: SGD
  • GPI anchor metabolic process Source: SGD
  • protein retention in ER lumen Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30435-MONOMER.

Protein family/group databases

ESTHERiyeast-BST1. PGAP1.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI inositol-deacylase (EC:3.1.-.-)
Alternative name(s):
Bypass of SEC30 protein 1
Gene namesi
Name:BST1
Ordered Locus Names:YFL025C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL025C.
SGDiS000001869. BST1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Transmembranei708 – 728HelicalSequence analysisAdd BLAST21
Transmembranei749 – 769HelicalSequence analysisAdd BLAST21
Transmembranei804 – 824HelicalSequence analysisAdd BLAST21
Transmembranei872 – 892HelicalSequence analysisAdd BLAST21
Transmembranei920 – 940HelicalSequence analysisAdd BLAST21
Transmembranei957 – 977HelicalSequence analysisAdd BLAST21
Transmembranei985 – 1005HelicalSequence analysisAdd BLAST21
Transmembranei1008 – 1028HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026761 – 1029GPI inositol-deacylaseAdd BLAST1029

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi19N-linked (GlcNAc...)Sequence analysis1
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi302N-linked (GlcNAc...)Sequence analysis1
Glycosylationi447N-linked (GlcNAc...)Sequence analysis1
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi485N-linked (GlcNAc...)Sequence analysis1
Glycosylationi565N-linked (GlcNAc...)Sequence analysis1
Glycosylationi651N-linked (GlcNAc...)Sequence analysis1
Glycosylationi668N-linked (GlcNAc...)Sequence analysis1
Glycosylationi694N-linked (GlcNAc...)Sequence analysis1
Glycosylationi918N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP43571.
PRIDEiP43571.

Interactioni

Protein-protein interaction databases

BioGridi31121. 205 interactors.
DIPiDIP-7407N.
IntActiP43571. 4 interactors.
MINTiMINT-1361319.

Structurei

3D structure databases

ProteinModelPortaliP43571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI inositol-deacylase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016484.
HOGENOMiHOG000248564.
InParanoidiP43571.
KOiK05294.
OMAiANNNPLW.
OrthoDBiEOG092C2M9W.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR012908. PGAP1-like.
[Graphical view]
PfamiPF07819. PGAP1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIRRLVSVI TRPIINKVNS SGQYSRVLAT REDQDKASPK YMNNDKIAKK
60 70 80 90 100
PYTYRLFSIL GILSICSLLL ISLLKPFNGA DAPQCESIYM FPSYARIDGF
110 120 130 140 150
DERYTPLAHK YHLYLYREQS VDREPLNGDE LQLDGIPVLF IPGNAGSFRQ
160 170 180 190 200
CRSIASACSN IYFDSNTRAT LRNENVRNLD FFTADFNEDF TAFHGETMLD
210 220 230 240 250
QAEYLNDAIK YILSLYERTP DYPHPKPQSV IIVGHSMGGI VSRVMLTLKN
260 270 280 290 300
HVPGSISTIL TLSSPHAASP VTFDGDILKL YKNTNEYWRK QLSQNDSFFS
310 320 330 340 350
KNISLVSITG GILDTTLPAD YASVEDLVSL ENGFTSFTTT IPDVWTPIDH
360 370 380 390 400
LAIVWCKQLR EVLARLLLES IDASKPEKVK PLNQRLQIAR KLLLSGFEDY
410 420 430 440 450
SWMNSKLNYP QENLQEFSDN FFSDYATLEM NDVLDFEMFN LEKWHNNYTK
460 470 480 490 500
INIPSNISST EHLHFTLLTS LDMPMIYFCT ESRVNLSCIT AVDSILTVPR
510 520 530 540 550
SSKDTQFAAD SSFGEAKNPF KAVSVGKNIL QKYDYLMISK PTYGEFSEQE
560 570 580 590 600
GMEDNQGFLL ALLRNVSNVQ IVNTTPSQIL LFGEQLHLDG KDIEQVISFS
610 620 630 640 650
NLWDSLLSYK LETKIEASNE GIASEETLFQ PFIRQWVYEP FESKWHLNII
660 670 680 690 700
NKSLDINMHN VAPFIPLNES EPRSLQLSFF IPPGMSLEAK MTINWSLTLK
710 720 730 740 750
MLFIRYRLAL ASFPVAFIAL VLSYQFYWYN KTSEFPSFDS TLGYILRKHG
760 770 780 790 800
ILMFFTLFLA SPVVNNKLVQ RILYLLDPVG LNYPFLLSER NMHANFYYLG
810 820 830 840 850
IRDWFMSTIG ILFGVMTVGL LALVSKIFGS LEILVIFLQR KLSKKNTEDK
860 870 880 890 900
EAFDTIEHKA YGKGRLMASV LLLLLVFFHI PYQMAFVISL VIQIATCIRV
910 920 930 940 950
ALLKLSNNEQ KLNLLNYNMT LLLLLLFVSA INIPIIIVFL HNVAIKWETS
960 970 980 990 1000
FRSHHNILAV APIIFLVGNN SIFKMPNSVP LDTWDGKVTI ILFVYLTVFS
1010 1020
FIYGIRNLYW IHHLVNIICA WLLFFETIH
Length:1,029
Mass (Da):117,755
Last modified:November 1, 1995 - v1
Checksum:i10B77B212E5AD686
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09213.1.
BK006940 Genomic DNA. Translation: DAA12415.1.
PIRiS56229.
RefSeqiNP_116628.1. NM_001179941.1.

Genome annotation databases

EnsemblFungiiBAA09213; BAA09213; BAA09213.
YFL025C; YFL025C; YFL025C.
GeneIDi850519.
KEGGisce:YFL025C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09213.1.
BK006940 Genomic DNA. Translation: DAA12415.1.
PIRiS56229.
RefSeqiNP_116628.1. NM_001179941.1.

3D structure databases

ProteinModelPortaliP43571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31121. 205 interactors.
DIPiDIP-7407N.
IntActiP43571. 4 interactors.
MINTiMINT-1361319.

Protein family/group databases

ESTHERiyeast-BST1. PGAP1.

Proteomic databases

MaxQBiP43571.
PRIDEiP43571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09213; BAA09213; BAA09213.
YFL025C; YFL025C; YFL025C.
GeneIDi850519.
KEGGisce:YFL025C.

Organism-specific databases

EuPathDBiFungiDB:YFL025C.
SGDiS000001869. BST1.

Phylogenomic databases

GeneTreeiENSGT00390000016484.
HOGENOMiHOG000248564.
InParanoidiP43571.
KOiK05294.
OMAiANNNPLW.
OrthoDBiEOG092C2M9W.

Enzyme and pathway databases

BioCyciYEAST:G3O-30435-MONOMER.

Miscellaneous databases

PROiP43571.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR012908. PGAP1-like.
[Graphical view]
PfamiPF07819. PGAP1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBST1_YEAST
AccessioniPrimary (citable) accession number: P43571
Secondary accession number(s): D6VTK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.