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Protein

GPI inositol-deacylase

Gene

BST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins. Required for the transport of misfolded protein to the Golgi, although dipensable for the transport of many normal proteins.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei236 – 2361By similarity

GO - Molecular functioni

  • phosphatidylinositol deacylase activity Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • ER to Golgi vesicle-mediated transport Source: SGD
  • GPI anchor metabolic process Source: SGD
  • intracellular protein transport Source: InterPro
  • protein retention in ER lumen Source: SGD
  • vesicle organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30435-MONOMER.

Protein family/group databases

ESTHERiyeast-BST1. PGAP1.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI inositol-deacylase (EC:3.1.-.-)
Alternative name(s):
Bypass of SEC30 protein 1
Gene namesi
Name:BST1
Ordered Locus Names:YFL025C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL025C.
SGDiS000001869. BST1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei57 – 7721HelicalSequence analysisAdd
BLAST
Transmembranei708 – 72821HelicalSequence analysisAdd
BLAST
Transmembranei749 – 76921HelicalSequence analysisAdd
BLAST
Transmembranei804 – 82421HelicalSequence analysisAdd
BLAST
Transmembranei872 – 89221HelicalSequence analysisAdd
BLAST
Transmembranei920 – 94021HelicalSequence analysisAdd
BLAST
Transmembranei957 – 97721HelicalSequence analysisAdd
BLAST
Transmembranei985 – 100521HelicalSequence analysisAdd
BLAST
Transmembranei1008 – 102821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029GPI inositol-deacylasePRO_0000202676Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...)Sequence analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence analysis
Glycosylationi447 – 4471N-linked (GlcNAc...)Sequence analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence analysis
Glycosylationi485 – 4851N-linked (GlcNAc...)Sequence analysis
Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence analysis
Glycosylationi651 – 6511N-linked (GlcNAc...)Sequence analysis
Glycosylationi668 – 6681N-linked (GlcNAc...)Sequence analysis
Glycosylationi694 – 6941N-linked (GlcNAc...)Sequence analysis
Glycosylationi918 – 9181N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP43571.

Interactioni

Protein-protein interaction databases

BioGridi31121. 205 interactions.
DIPiDIP-7407N.
IntActiP43571. 4 interactions.
MINTiMINT-1361319.

Structurei

3D structure databases

ProteinModelPortaliP43571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI inositol-deacylase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016484.
HOGENOMiHOG000248564.
InParanoidiP43571.
KOiK05294.
OMAiVPETHGF.
OrthoDBiEOG7Q2NFT.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR012908. PGAP1-like.
[Graphical view]
PfamiPF07819. PGAP1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIRRLVSVI TRPIINKVNS SGQYSRVLAT REDQDKASPK YMNNDKIAKK
60 70 80 90 100
PYTYRLFSIL GILSICSLLL ISLLKPFNGA DAPQCESIYM FPSYARIDGF
110 120 130 140 150
DERYTPLAHK YHLYLYREQS VDREPLNGDE LQLDGIPVLF IPGNAGSFRQ
160 170 180 190 200
CRSIASACSN IYFDSNTRAT LRNENVRNLD FFTADFNEDF TAFHGETMLD
210 220 230 240 250
QAEYLNDAIK YILSLYERTP DYPHPKPQSV IIVGHSMGGI VSRVMLTLKN
260 270 280 290 300
HVPGSISTIL TLSSPHAASP VTFDGDILKL YKNTNEYWRK QLSQNDSFFS
310 320 330 340 350
KNISLVSITG GILDTTLPAD YASVEDLVSL ENGFTSFTTT IPDVWTPIDH
360 370 380 390 400
LAIVWCKQLR EVLARLLLES IDASKPEKVK PLNQRLQIAR KLLLSGFEDY
410 420 430 440 450
SWMNSKLNYP QENLQEFSDN FFSDYATLEM NDVLDFEMFN LEKWHNNYTK
460 470 480 490 500
INIPSNISST EHLHFTLLTS LDMPMIYFCT ESRVNLSCIT AVDSILTVPR
510 520 530 540 550
SSKDTQFAAD SSFGEAKNPF KAVSVGKNIL QKYDYLMISK PTYGEFSEQE
560 570 580 590 600
GMEDNQGFLL ALLRNVSNVQ IVNTTPSQIL LFGEQLHLDG KDIEQVISFS
610 620 630 640 650
NLWDSLLSYK LETKIEASNE GIASEETLFQ PFIRQWVYEP FESKWHLNII
660 670 680 690 700
NKSLDINMHN VAPFIPLNES EPRSLQLSFF IPPGMSLEAK MTINWSLTLK
710 720 730 740 750
MLFIRYRLAL ASFPVAFIAL VLSYQFYWYN KTSEFPSFDS TLGYILRKHG
760 770 780 790 800
ILMFFTLFLA SPVVNNKLVQ RILYLLDPVG LNYPFLLSER NMHANFYYLG
810 820 830 840 850
IRDWFMSTIG ILFGVMTVGL LALVSKIFGS LEILVIFLQR KLSKKNTEDK
860 870 880 890 900
EAFDTIEHKA YGKGRLMASV LLLLLVFFHI PYQMAFVISL VIQIATCIRV
910 920 930 940 950
ALLKLSNNEQ KLNLLNYNMT LLLLLLFVSA INIPIIIVFL HNVAIKWETS
960 970 980 990 1000
FRSHHNILAV APIIFLVGNN SIFKMPNSVP LDTWDGKVTI ILFVYLTVFS
1010 1020
FIYGIRNLYW IHHLVNIICA WLLFFETIH
Length:1,029
Mass (Da):117,755
Last modified:November 1, 1995 - v1
Checksum:i10B77B212E5AD686
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09213.1.
BK006940 Genomic DNA. Translation: DAA12415.1.
PIRiS56229.
RefSeqiNP_116628.1. NM_001179941.1.

Genome annotation databases

EnsemblFungiiBAA09213; BAA09213; BAA09213.
YFL025C; YFL025C; YFL025C.
GeneIDi850519.
KEGGisce:YFL025C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09213.1.
BK006940 Genomic DNA. Translation: DAA12415.1.
PIRiS56229.
RefSeqiNP_116628.1. NM_001179941.1.

3D structure databases

ProteinModelPortaliP43571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31121. 205 interactions.
DIPiDIP-7407N.
IntActiP43571. 4 interactions.
MINTiMINT-1361319.

Protein family/group databases

ESTHERiyeast-BST1. PGAP1.

Proteomic databases

MaxQBiP43571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09213; BAA09213; BAA09213.
YFL025C; YFL025C; YFL025C.
GeneIDi850519.
KEGGisce:YFL025C.

Organism-specific databases

EuPathDBiFungiDB:YFL025C.
SGDiS000001869. BST1.

Phylogenomic databases

GeneTreeiENSGT00390000016484.
HOGENOMiHOG000248564.
InParanoidiP43571.
KOiK05294.
OMAiVPETHGF.
OrthoDBiEOG7Q2NFT.

Enzyme and pathway databases

BioCyciYEAST:G3O-30435-MONOMER.

Miscellaneous databases

NextBioi966248.
PROiP43571.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR012908. PGAP1-like.
[Graphical view]
PfamiPF07819. PGAP1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations."
    Elrod-Erickson M.J., Kaiser C.A.
    Mol. Biol. Cell 7:1043-1058(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION.
  4. "Distinct retrieval and retention mechanisms are required for the quality control of endoplasmic reticulum protein folding."
    Vashist S., Kim W., Belden W.J., Spear E.D., Barlowe C., Ng D.T.W.
    J. Cell Biol. 155:355-368(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p."
    Tanaka S., Maeda Y., Tashima Y., Kinoshita T.
    J. Biol. Chem. 279:14256-14263(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol-anchored proteins."
    Fujita M., Yoko-o T., Jigami Y.
    Mol. Biol. Cell 17:834-850(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiBST1_YEAST
AccessioniPrimary (citable) accession number: P43571
Secondary accession number(s): D6VTK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.